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JoVE Journal Biochemistry
En elektrochemiluminescence-baserad analys för MeCP2 Protein Varianter
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En elektrochemiluminescence-baserad analys för MeCP2 Protein Varianter

Article DOI: 10.3791/61054-v • 07:44 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 2, 3, 1, 1, 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

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Electrochemiluminescence immunoassay (ECLIA) är en ny metod för kvantitativ detektion av endogena och exogent tillämpas MeCP2 protein varianter, som producerar mycket kvantitativa, exakta och reproducerbara mätningar med låg inom- och inter-assay fel över ett brett arbetsområde. Här beskrivs protokollet för MeCP2-ECLIA i ett 96-brunnsformat.

Tags

Biokemi ECLIA Sulfo-TAG MeCP2 TAT fusion protein TAT-MeCP2 Rett syndrom proteinersättningsterapi
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