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Journal / Biochemistry / MeCP2タンパク質変異体に対するエレクトロケミセンスベースアッセイ…
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Biochemistry

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JoVE Journal Biochemistry
MeCP2タンパク質変異体に対するエレクトロケミセンスベースアッセイ
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
Journal (Biochemistry)
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Journal (Biochemistry)
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
 
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MeCP2タンパク質変異体に対するエレクトロケミセンスベースアッセイ

Article DOI: 10.3791/61054-v • 07:44 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 2, 3, 1, 1, 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

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エレクトロケミ発光免疫測定法(ECLIA)は、内因性および外因的に適用されたMeCP2タンパク質変異体の定量的検出のための新しいアプローチであり、幅広い作業範囲での低いイントラおよびインターアッセイ誤差で定量的、正確、再現性の高い測定を行います。ここでは、96 ウェル形式での MeCP2-ECLIA のプロトコルについて説明します。

Tags

生化学、問題159、ECLIA、スルフォタグ、MeCP2、TAT融合タンパク質、TAT-MeCP2、レット症候群、タンパク質置換療法
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