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Biochemistry

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JoVE Journal Biochemistry
Ein Electrochemilumineszenz-basierter Assay für MeCP2-Proteinvarianten
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Ein Electrochemilumineszenz-basierter Assay für MeCP2-Proteinvarianten

Article DOI: 10.3791/61054-v • 07:44 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 2, 3, 1, 1, 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

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Der Elektrochemilumineszenz-Immunoassay (ECLIA) ist ein neuartiger Ansatz für den quantitativen Nachweis endogener und exogen angewendeter MeCP2-Proteinvarianten, der hochquantitative, genaue und reproduzierbare Messungen mit geringem Intra- und Inter-Assay-Fehler über einen weiten Arbeitsbereich erzeugt. Hier wird das Protokoll für die MeCP2-ECLIA im 96-Well-Format beschrieben.

Tags

Biochemie Ausgabe 159 ECLIA Sulfo-TAG MeCP2 TAT Fusionsprotein TAT-MeCP2 Rett-Syndrom Proteinersatztherapie
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