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Journal / Developmental Biology / Isolering af endokardie- og…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering af endokardie- og koronarenendotelceller fra den ventrikelfrie frie væg af rottehjertet
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Isolering af endokardie- og koronarenendotelceller fra den ventrikelfrie frie væg af rottehjertet

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Vi præsenterer en protokol for isolering af endokardie- og koronarenendotelceller fra rottehjerter gennem sekventiel vævsfordøjelse i en fordøjelsesbuffer, cellesamling fra tilbagevendende centrifugecyklusser og cellerensning ved hjælp af anti-rotte CD31 mikroperler.

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Udviklingsbiologi Udgave 158 endokardiet koronar endotel isolation hjerte rotte CD31
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