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Journal / Developmental Biology / Isolierung von Endokardialen und koronaren…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolierung von Endokardialen und koronaren Endothelzellen aus der ventrikulären freien Wand des Rattenherzes
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Isolierung von Endokardialen und koronaren Endothelzellen aus der ventrikulären freien Wand des Rattenherzes

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Wir präsentieren ein Protokoll zur Isolierung von endokardialen und koronaren Endothelzellen aus Rattenherzen durch sequentielle Gewebeverdauung in einem Verdauungspuffer, Zellsammlung aus rezidivierenden Zentrifugenzyklen und Zellreinigung mit rattenfeindlichen CD31-Mikroperlen.

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Entwicklungsbiologie Ausgabe 158 Endokard koronares Endothel Isolation Herz Ratte CD31
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