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Journal / Developmental Biology / Isolering av endokardiale og koronar…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering av endokardiale og koronar endotelceller fra Ventricular Free Wall of the Rat Heart
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Isolering av endokardiale og koronar endotelceller fra Ventricular Free Wall of the Rat Heart

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Vi presenterer en protokoll for isolering av endokardiale og koronar endotelceller fra rottehjerter gjennom sekvensiell vevfordøyelse i fordøyelsesbuffer, cellesamling fra tilbakevendende sentrifugesykluser og cellerensing ved hjelp av antirotte CD31 mikroperler.

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Utviklingsbiologi Utgave 158 endokardiet koronar endotel isolasjon hjerte rotte CD31
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