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Journal / Developmental Biology / Isolamento das Células Endocárdicas e Coronárias…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Isolamento das Células Endocárdicas e Coronárias da Parede Livre Ventricular do Coração de Rato
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Isolamento das Células Endocárdicas e Coronárias da Parede Livre Ventricular do Coração de Rato

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Apresentamos um protocolo para o isolamento de células endocárdicas e coronárias de corações de ratos através da digestão sequencial de tecidos em um tampão de digestão, coleta celular de ciclos de centrífuga recorrentes e purificação celular usando microesferas CD31 anti-ratos.

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Biologia do Desenvolvimento Edição 158 endocárdio endotélio coronário isolamento coração rato CD31
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