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Journal / Developmental Biology / Aislamiento de células endoteliales endocardiales …
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Developmental Biology

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JoVE Journal Developmental Biology
Aislamiento de células endoteliales endocardiales y coronarias de la pared libre ventricular del corazón de rata
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Aislamiento de células endoteliales endocardiales y coronarias de la pared libre ventricular del corazón de rata

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Presentamos un protocolo para el aislamiento de células endoteliales endocardiales y coronarias de corazones de rata a través de la digestión secuencial del tejido en un tampón de digestión, la recolección celular de ciclos de centrífuga recurrentes y la purificación celular mediante microperlas CD31 anti-rata.

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Biología del desarrollo Número 158 endocardio endotelio coronario aislamiento corazón rata CD31
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