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Biochemistry

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JoVE Journal Biochemistry
Bedömning av cellulär oxidation med hjälp av ett subcellulärt fackspecifikt Redox-sensitive green fluorescerande protein
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Bedömning av cellulär oxidation med hjälp av ett subcellulärt fackspecifikt Redox-sensitive green fluorescerande protein

Article DOI: 10.3791/61229-v • 06:10 min • June 18th, 2020
June 18th, 2020 •
1,2, 1, 1, 1
1Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

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Detta protokoll beskriver bedömningen av subcellulära fack-specifika redox status inom cellen. En redox-känslig fluorescerande sond möjliggör bekväm ratiometrisk analys i intakta celler.

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Biokemi Utgåva 160 redox känsliga gröna fluorescerande protein (roGFP) cytosolic roGFP cystein/cystin ratio levande cell imaging flödescytometri oxidation/reduktion
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