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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry
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Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Article DOI: 10.3791/61436-v • 08:07 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Biological Sciences, University of North Carolina at Charlotte

Summary
Automatic Translation

This protocol describes a method for culturing primary hippocampal neurons from embryonic mouse brain. The expression of major histocompatibility complex class I on the extracellular surface of cultured neurons is then assessed by flow cytometric analysis.

Tags

Flow Cytometry MHC Class I Expression Primary Neurons Murine Hippocampal Neurons NC2 Immunostaining Protein Structure Denaturing Conditions Immune Responses Synaptic Connections Embryonic Brain Dissection Culturing Process Isolation Of Hippocampus Meninges Removal
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