焦磷酸测序:一个准确的基因分型的简单方法

Biology
 

Summary

焦磷酸测序(R)是其中一个最彻底而又简单的方法用于分析基因多态性。此方法具有快速,高效的单核苷酸多态性的评价,包括很多临床相关基因多态性。焦磷酸测序的技术和方法进行了解释。

Cite this Article

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King, C., Scott-Horton, T. Pyrosequencing: A Simple Method for Accurate Genotyping. J. Vis. Exp. (11), e630, doi:10.3791/630 (2008).

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Abstract

完成人类基因组计划所提供的信息丰富的第一手的药理学研究的好处。有了这样一个大量的可用数据,基因分型方法的爆炸。焦磷酸测序(R)是其中一个最彻底而又简单的方法用于分析基因多态性。它还具有识别能力,三等位基因,INDELS,短重复序列多态性,同时与甲基化或样本评估确定的等位基因百分比。此外,还有一个标准化的控制序列,提供了内部质量控制。此方法具有快速,高效的单核苷酸多态性的评价,包括很多临床相关基因多态性。焦磷酸测序的技术和方法进行了解释。

Protocol

这个实验方法的完整的文本协议,在斯普林格协议。

Comments

7 Comments

  1. thank's for u'r share. this is usefull.

    Reply
    Posted by: Anonymous
    August 18, 2008 - 11:45 PM
  2. hello!your video is great ~ I can not get the complete text protocol for this experimental in Springer Protocols, could you please send it to my e-mail :qqsvery@hotmail.com. I am a postgraduate from JiangXi Agriculture University in China .thanks for your help !

    Reply
    Posted by: Qiu Q.
    March 28, 2010 - 7:16 AM
  3. How can you call the this the Journal of visualized experiments when I cannot for the life of me get the video started????????????



    Reply
    Posted by: Erica L. E.
    June 20, 2011 - 3:10 PM
  4. pyrosequencing is latest method? or it being used or nt?

    Reply
    Posted by: Anonymous
    December 24, 2011 - 12:47 AM
  5. hi so much thanks to your effort to explain pyrosequencing it was great please send it to my account

    Reply
    Posted by: Anonymous
    March 17, 2012 - 4:20 AM
  6. hi so much thanks to your effort to explain pyrosequencing it was great please send it to my account ladan_va@yahoo.com

    Reply
    Posted by: Anonymous
    March 17, 2012 - 4:21 AM
  7. All these videos are useful, Thank you so much

    Reply
    Posted by: Mo A.
    September 4, 2012 - 9:55 AM

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