Pyrosequencing: A Simple Method for Accurate Genotyping

Published 1/08/2008
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Biology
 

Summary

Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.

Cite this Article

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King, C., Scott-Horton, T. Pyrosequencing: A Simple Method for Accurate Genotyping. J. Vis. Exp. (11), e630, doi:10.3791/630 (2008).

Abstract

Pharmacogenetic research benefits first-hand from the abundance of information provided by the completion of the Human Genome Project. With such a tremendous amount of data available comes an explosion of genotyping methods. Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. It also has the ability to identify tri-allelic, indels, short-repeat polymorphisms, along with determining allele percentages for methylation or pooled sample assessment. In addition, there is a standardized control sequence that provides internal quality control. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.

Protocol

The complete text protocol for this experimental approach is available in Springer Protocols.

Disclosures

The authors have nothing to disclose.

Comments

7 Comments

  1. thank's for u'r share. this is usefull.

    Reply
    Posted by: Anonymous
    August 18, 2008 - 11:45 PM
  2. hello!your video is great ~ I can not get the complete text protocol for this experimental in Springer Protocols, could you please send it to my e-mail :qqsvery@hotmail.com. I am a postgraduate from JiangXi Agriculture University in China .thanks for your help !

    Reply
    Posted by: Qiu Q.
    March 28, 2010 - 7:16 AM
  3. How can you call the this the Journal of visualized experiments when I cannot for the life of me get the video started????????????



    Reply
    Posted by: Erica L. E.
    June 20, 2011 - 3:10 PM
  4. pyrosequencing is latest method? or it being used or nt?

    Reply
    Posted by: Anonymous
    December 24, 2011 - 12:47 AM
  5. hi so much thanks to your effort to explain pyrosequencing it was great please send it to my account

    Reply
    Posted by: Anonymous
    March 17, 2012 - 4:20 AM
  6. hi so much thanks to your effort to explain pyrosequencing it was great please send it to my account ladan_va@yahoo.com

    Reply
    Posted by: Anonymous
    March 17, 2012 - 4:21 AM
  7. All these videos are useful, Thank you so much

    Reply
    Posted by: Mo A.
    September 4, 2012 - 9:55 AM

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