Journal Monthly Highlights: May 2019

1JoVE Content Production

Manipulation of Color Patterns in Jumping Spiders for Use in Behavioral Experiments

Malika Ihle1, Lisa Anne Taylor1,2

The goal of this protocol is to manipulate the color patterns of jumping spiders and other very small arthropods with paint in order to study questions related to sexual selection, sexual cannibalism, predation, aposematism, or any other field of animal coloration.

Spotlighting Customers' Visual Attention at the Stock, Shelf and Store Levels with the 3S Model

Tobias Otterbring1,2, Erik Wästlund2, Poja Shams3

This article presents a new conceptualization of the in-store search process, the 3S Model, which captures customers’ visual attention at three distinct levels of analysis: Stock, Shelf, and Store. We illustrate the usefulness of our conceptualization through three eye-tracking studies, one from each level of analysis in the 3S Model.

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Carlos Flores1,2,3, Paula Tamagnini1,2,4

Here, protocols for the isolation of cyanobacterial released carbohydrate polymers and isolation of their exoproteomes are described. Both procedures embody key steps to obtain polymers or proteins with high purity degrees that can be used for further analysis or applications. They can also be easily adapted according to specific user needs.

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Tiago Franco de Oliveira*1,2, Antonio Anax Falcão de Oliveira*1, Miriam Lemos3, Mariana Veras3, Paulo Hilário Nascimento Saldiva3,4, Marisa Helena Gennari de Medeiros5, Paolo Di Mascio5, Ana Paula de Melo Loureiro1
* These authors contributed equally

We describe here methods for sensitive and accurate quantification of the lesions 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 1,N6-etheno-2'-deoxyadenosine (1,N6-dAdo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-dGuo) in DNA. The methods were applied to the assessment of the effects of ambient fine particulate matter (PM2.5) in tissues (lung, liver and kidney) of exposed A/J mice.