Journal Monthly Highlights: May 2019

1, 1
1JoVE Content Production

Manipulation of Color Patterns in Jumping Spiders for Use in Behavioral Experiments

Malika Ihle1, Lisa Anne Taylor1,2
1Department of Entomology and Nematology, University of Florida, 2Florida Museum of Natural History, University of Florida

The goal of this protocol is to manipulate the color patterns of jumping spiders and other very small arthropods with paint in order to study questions related to sexual selection, sexual cannibalism, predation, aposematism, or any other field of animal coloration.

Spotlighting Customers' Visual Attention at the Stock, Shelf and Store Levels with the 3S Model

Tobias Otterbring1,2, Erik Wästlund2, Poja Shams3
1Department of Management/MAPP, Aarhus University, 2Department of Psychology & CTF, Service Research Center, Karlstad University, 3Department of Business Administration & CTF, Karlstad University

This article presents a new conceptualization of the in-store search process, the 3S Model, which captures customers’ visual attention at three distinct levels of analysis: Stock, Shelf, and Store. We illustrate the usefulness of our conceptualization through three eye-tracking studies, one from each level of analysis in the 3S Model.

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Carlos Flores1,2,3, Paula Tamagnini1,2,4
1Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude, Universidade do Porto, 2Instituto de Biologia Celular e Molecular, Universidade do Porto, 3Instituto de Ciências Biomédicas Abel Salazar, 4Departamento de Biologia, Faculdade de Ciências, Universidade do Porto

Here, protocols for the isolation of cyanobacterial released carbohydrate polymers and isolation of their exoproteomes are described. Both procedures embody key steps to obtain polymers or proteins with high purity degrees that can be used for further analysis or applications. They can also be easily adapted according to specific user needs.

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Tiago Franco de Oliveira*1,2, Antonio Anax Falcão de Oliveira*1, Miriam Lemos3, Mariana Veras3, Paulo Hilário Nascimento Saldiva3,4, Marisa Helena Gennari de Medeiros5, Paolo Di Mascio5, Ana Paula de Melo Loureiro1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
* These authors contributed equally

We describe here methods for sensitive and accurate quantification of the lesions 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 1,N6-etheno-2'-deoxyadenosine (1,N6-dAdo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-dGuo) in DNA. The methods were applied to the assessment of the effects of ambient fine particulate matter (PM2.5) in tissues (lung, liver and kidney) of exposed A/J mice.

PUBLISH WITH JoVE RECOMMEND JoVE