Journal Monthly Highlights: October 2019

1JoVE Content Development

Applications for Open Source Microplate-Compatible Illumination Panels

Pierre Baillargeon1, Timothy P. Spicer1, Louis Scampavia1

Microplate Assistive Pipetting Light Emitter (M.A.P.L.E.) is a computer-driven device that systematically illuminates microtiter wells to provide guidance for the manual preparation of microplates.  M.A.P.L.E. improves the accuracy of microplate preparation while automating data recordkeeping.  In addition, it can assist with examining microplate quality or aid in the detection of errors.   

Measuring Neural Mechanisms Underlying Sleep-Dependent Memory Consolidation During Naps in Early Childhood

Tamara Allard*1, Tracy Riggins*1, Arcadia Ewell1, Benjamin Weinberg1, Sanna Lokhandwala2, Rebecca M. C. Spencer2,3
* These authors contributed equally

This protocol describes methods used to examine neural mechanisms underlying sleep-dependent memory consolidation during naps in early childhood. It includes procedures for examining the effect of sleep on behavioral memory performance, as well as the application and recording of both polysomnography and actigraphy.

Training Dogs for Awake, Unrestrained Functional Magnetic Resonance Imaging

Lily R. Strassberg*1, L. Paul Waggoner*2, Gopikrishna Deshpande*1,3,4,5, Jeffrey S. Katz*1,3,4,5
* These authors contributed equally

Magnetic resonance imaging (MRI) on unrestrained awake dogs is a new method with several advantages over imaging with physical or chemical restraint. This protocol introduces a cost-effective training method that minimizes training in the MRI environment, which can be expensive, and maximizes the subject pool available for canine functional MRI.

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Laurel R. Yohe*1,2, Paolo Devanna*3, Kalina T.J. Davies4, Joshua H.T. Potter4, Stephen J. Rossiter4, Emma C. Teeling5, Sonja C. Vernes*3,6, Liliana M. Dávalos*2,7
* These authors contributed equally

This is a protocol for the optimal tissue preparation for genomic, transcriptomic, and proteomic analyses of bats caught in the wild. It includes protocols for bat capture and dissection, tissue preservation, and cell culturing of bat tissue.