Journal Monthly Highlights: November 2019

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1JoVE Content Development

CO2-Lasertonsillotomy Under Local Anesthesia in Adults

Justin E.R.E. Wong Chung1,2, Noud van Helmond3, Rozemarie van Geet1, Peter Paul G. van Benthem2, Henk M. Blom1,2
1Department of Otolaryngology, HagaZiekenhuis, 2Department of Otolaryngology, Leiden University Medical Center, 3Department of Anesthesiology, Cooper Medical School of Rowan University, Cooper University Hospital

CO2-lasertonsillotomy under local anesthesia is an interesting alternative treatment method for tonsillectomy under general anesthesia for tonsil-related complaints in adults. This report presents a step-by-step protocol detailing the execution of CO2-lasertonsillotomy under local anesthesia.

Transcranial Direct Current Stimulation for Online Gamers

Sang Hoon Lee*1, Jooyeon Jamie Im*2, Jin Kyoung Oh2, Eun Kyoung Choi2, Sujung Yoon3, Marom Bikson4, In-Uk Song5, Hyeonseok Jeong2, Yong-An Chung2
1Department of Radiology, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Department of Radiology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 3Department of Brain and Cognitive Sciences, Ewha Womans University, 4Department of Biomedical Engineering, The City College of New York, 5Department of Neurology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea
* These authors contributed equally

We present a protocol and a feasibility study for applying transcranial direct current stimulation (tDCS) and neuroimaging assessment in online gamers.

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Kai Zhang1,2, Nima Davoudzadeh1,2, Guillaume Ducourthial1,2, Bryan Q. Spring1,2,3
1Translational Biophotonics Cluster, Northeastern University, 2Department of Physics, Northeastern University, 3Department of Bioengineering, Northeastern University

A method is presented to build a custom low-cost, mode-locked femtosecond fiber laser for potential applications in multiphoton microscopy, endoscopy, and photomedicine. This laser is built using commercially available parts and basic splicing techniques.

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Christina Rou Hsu1,2, Rain Xiong1,2, Kenji Sugioka1,2
1Department of Zoology, The University of British Columbia, 2Life Sciences Institute, The University of British Columbia

Tissue complexities of multicellular systems confound the identification of causal relationship between extracellular cues and individual cellular behaviors. Here, we present a method to study the direct link between contact-dependent cues and division axes using C. elegans embryo blastomeres and adhesive polystyrene beads.

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