拆装时间推移显微镜监测肌动蛋白

Published 11/08/2006
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Biology

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Summary

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Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

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Abstract

Protocol

  1. 构造一个在视频流室。确保封口膜密封紧,用洗净的盖玻片。
  2. 孵育肌动蛋白结合剂5-10“室。
  3. 座具有阻止蛋白质的非特异性结合位点上的玻璃盖玻片。
  4. 肌动蛋白聚合箱内流淌在G -肌动蛋白聚合缓冲区。
  5. 冲蚀unpolymerized肌动蛋白在过剩缓冲区流动。

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Comments

1 Comment

  1. What is the second pair of shorter parafilm strips for? Why do you polymerize in the chamber and how? G-actin in polymerization buffer. Is't that F-actin? What is the actin binding agent and blocking protein and their buffers and their concentrations?

    Reply
    Posted by: Anonymous
    July 28, 2009 - 2:41 PM

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