Actin Disassembly निगरानी समय चूक माइक्रोस्कोपी के साथ

Biology

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Summary

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Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

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Abstract

Protocol

  1. एक प्रवाह के रूप में वीडियो में दिखाया गया है कक्ष का निर्माण. सुनिश्चित करें कि parafilm मुहर तंग है और धोया coverslips का उपयोग करें.
  2. 5-10 'के लिए कक्ष में सेते actin बाध्यकारी एजेंट.
  3. एक अवरुद्ध प्रोटीन के साथ गैर विशिष्ट कांच coverslip पर बाध्यकारी साइटों को ब्लॉक.
  4. कक्ष के अंदर बफर polymerizing में जी actin में बह polymerize actin.
  5. वार्शआउट अतिरिक्त बफर में बह द्वारा unpolymerized actin.

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Comments

1 Comment

  1. What is the second pair of shorter parafilm strips for? Why do you polymerize in the chamber and how? G-actin in polymerization buffer. Is't that F-actin? What is the actin binding agent and blocking protein and their buffers and their concentrations?

    Reply
    Posted by: Anonymous
    July 28, 2009 - 2:41 PM

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