Monitoraggio Actina Smontaggio con Time-lapse Microscopia


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Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

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  1. Costruire una camera di flusso, come mostrato nel video. Assicurarsi che la guarnizione parafilm è stretto e l'utilizzo coprioggetto lavato.
  2. Incubare actina-legante nella camera per '5-10.
  3. Blocco siti di legame non specifici sul vetrino di vetro con una proteina di blocco.
  4. Actina polimerizzano all'interno della camera di flusso in G-actina in polimerizzazione buffer.
  5. Washout actina non polimerizzato da che scorre in tampone in eccesso.

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1 Comment

  1. What is the second pair of shorter parafilm strips for? Why do you polymerize in the chamber and how? G-actin in polymerization buffer. Is't that F-actin? What is the actin binding agent and blocking protein and their buffers and their concentrations?

    Posted by: Anonymous
    July 28, 2009 - 2:41 PM

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