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JoVE Journal
Biology

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Aktin Co-Sedimentation Assay; für die Analyse von Protein-Bindung an F-Aktin
 
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Aktin Co-Sedimentation Assay; für die Analyse von Protein-Bindung an F-Aktin

Article DOI: 10.3791/690-v 07:54 min March 28th, 2008
March 28th, 2008

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Summary

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Click here for the English version.

Proteine ​​binden an filamentösen Aktin (F-Aktin) durch verschiedene Aktin-bindende Module. In diesem Video zeigen wir das Verfahren von Aktin co-Sedimentation, die eine in vitro-Assay routinemäßig verwendet, um Proteine ​​oder bestimmte Domänen, die F-Aktin binden zu analysieren.

Tags

Biochemie F-Aktin-Protein in-vitro-Bindung Ultrazentrifugation
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