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Biology

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Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection
 

Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection

Article DOI: 10.3791/700-v 06:01 min May 14th, 2008
May 14th, 2008

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Summary

Aphids are effective transmitters of plant viruses. Aphid microinjection of virus, the procedure we will show you today, is a technique allowing researchers to inject virus directly into the hemocoel of the aphid, bypassing the gut, one of the 2 major barriers for virus transmission in a circulative manner. The same technique is also used to inject dsRNA for RNAi.

Tags

Physiological Barriers Viral Transmission Aphids Microinjection Potato Loafroll Virus (PLRV) Luteoviridae Green Peach Aphid Plant Phloem Hemolymph Salivary Gland Physalis Plants PLRV Transmission Stunting Interveinal Chlorosis Yellowing Of Leaves Viral Transmission Prevention
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