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Single Cell elektroporation in vivo i den intakta växande hjärnan
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Single Cell elektroporation in vivo i den intakta växande hjärnan

Article DOI: 10.3791/705-v • 13:31 min • July 11th, 2008
July 11th, 2008 •
1,2, 1,2
1Brain Research Centre, University of British Columbia - UBC, 2Department of Cellular and Physiological Sciences, University of British Columbia - UBC

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Encelliga elektroporation (SCE) är en specialiserad teknik som möjliggör leverans av DNA eller andra makromolekyler i enskilda celler i intakta vävnader, inklusive in vivo förberedelser. Här har vi detalj förfarandet för SCE av en fluorescerande färg eller plasmid-DNA till nervceller i den intakta hjärnan i Xenopus laevis grodyngel.

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Neurovetenskap nummer 17 elektroporation gen leverans transfektion fluorescens märkning neuronala bildbehandling mikropipetten
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