예 VIVO 바이러스성 전달을 사용하여 헤어 대과에 GFP 타겟팅 표현

Biology
 

ERRATUM NOTICE

Summary

머리 뿌리는 세포 종류의 다중성을 포함하는 피부의 매우 복잡한 부위요입니다. 뿌리의 성장과 특정 줄기 세포에 의존 성장과 재흡수를 포함하여 유기체의 생명을 통해 지속적인 자전거 타기를 겪습. 속성을 변경하는 유전자와 줄기 세포에 의해 뿌리의 대상으로, 특히 머리 샤프트​​의 특성은, 논의했다. 헤어 대과 전달 시스템은 이러한 liposomes 및 유전자 치료에 대한 바이러스 벡터로 설명되어 있습니다. 머리 뿌리의 줄기 세포의 자연, 특히 그 pluripotency을 논의합니다.

Cite this Article

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Hoffman, R. M., Li, L. Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction. J. Vis. Exp. (13), e708, doi:10.3791/708 (2008).

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Abstract

머리 뿌리는 세포 종류의 다중성을 포함하는 피부의 매우 복잡한 부위요입니다. 뿌리의 성장과 특정 줄기 세포에 의존 성장과 재흡수를 포함하여 유기체의 생명을 통해 지속적인 자전거 타기를 겪습. 속성을 변경하는 유전자와 줄기 세포에 의해 뿌리의 대상으로, 특히 머리 샤프트​​의 특성은, 논의했다. 헤어 대과 전달 시스템은 이러한 liposomes 및 유전자 치료에 대한 바이러스 벡터로 설명되어 있습니다. 머리 뿌리의 줄기 세포의 자연, 특히 그 pluripotency을 논의합니다.

Protocol

예 생체내 바이러스 transductionplease를 사용하여 머리 뿌리에서 GFP의 대상 표현에 대한 자세한 내용을 보려면 다음 사이트를 방문 스프링거 프로토콜을 .

Erratum

Formal Correction: Erratum: Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
Posted by JoVE Editors on 03/07/2012. Citeable Link.

A correction was made to: Targeted expression of GFP in the hair follicle using ex vivo viral transduction. A revised abstract was republished due to a publisher error. The correct abstract is:

There are many cell types in the hair follicle, including hair matrix cells which form the hair shaft and stem cells which can initiate the hair shaft during early anagen, the growth phase of the hair cycle, as well as pluripotent stem cells that play a role in hair follicle growth but have the potential to differentiate to non-follicle cells such as neurons. These properties of the hair follicle are discussed. The various cell types of the hair follicle are potential targets for gene therapy. Gene delivery system for the hair follicle using viral vectors or liposomes for gene targeting to the various cell types in the hair follicle and the results obtained are also discussed.

This replaced:

The hair follicle is a highly complex appendage of the skin containing a multiplicity of cell types. The follicle undergoes constant cycling through the life of the organism including growth and resorption with growth dependent on specific stem cells. The targeting of the follicle by genes and stem cells to change its properties, in particular, the nature of the hair shaft is, discussed. Hair follicle delivery systems are described, such as liposomes and viral vectors for gene therapy. The nature of the hair follicle stem cells is discussed, in particular, its pluripotency.

Comments

4 Comments

  1. Has any progress been made regarding the production  of a product for the cosmetic market returning grey hair to original colouring as reported in the press some time ago?

    Reply
    Posted by: Anonymous
    October 5, 2008 - 2:05 AM
  2. Hair follicle is a part of the skin from where the old hairs can regrow. Electrolysis and Laser hair restoration are techniques to restore the hair on balding areas. The average growth rate of healthy hair follicles on the scalp is 400 micrometer per day.

    www.findhairhelp.com/electrolysis.html

    Reply
    Posted by: james h.
    June 20, 2009 - 3:46 AM
  3. Here is my question- Once we do this technique in human beings, say for suppose to modify the skin colour, how long it is going to stay with the patient? Another question- won't be the viral harmful for the patient. ?

    --Many thanks,
    Bipasha

    Reply
    Posted by: Anonymous
    December 3, 2009 - 2:41 AM
  4. can we use same method for human

    Reply
    Posted by: Anonymous
    May 10, 2010 - 5:54 AM

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