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Biology

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Principles of Site-Specific Recombinase (SSR) Technology
 

Principles of Site-Specific Recombinase (SSR) Technology

Article DOI: 10.3791/718-v 07:06 min May 29th, 2008
May 29th, 2008

Chapters

Summary

The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular biology, and has proven itself as a valuable tool for assessing gene function in transgenic animals. This interview discusses the mechanism of site specific recombination by Cyclization recombinase (Cre) and how the use of this enzyme has led to the development of conditional mutagenesis, which has significant advantages over traditional knock out strategies.

Tags

Site-specific Recombinase (SSR) Technology Gene Structure Manipulation Gene Function Exploration Molecular Biology Tool Cre/lox System Cre Recombinase LoxP Sites Recombination Event DNA Rearrangement Gene Deletion Tissue-specific Promoter
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