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Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
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Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Article DOI: 10.3791/742-v • 14:43 min • June 24th, 2008
June 24th, 2008 •
1, 1, 1
1Jonsson Comprehensive Cancer Center, University of California, Los Angeles

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We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta.

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Hematopoietic Stem Cells Placenta Isolation Analysis Self-renewal Blood Lineages Embryonic Development Pool Size Anatomical Origin Fetal Hematopoiesis Microenvironmental Niches Dissection Techniques Murine Placenta Developmental Stages HSC Pool Size Enzymatic Dissociation Mechanical Dissociation Flow Cytometry Functional Assays
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