Separazione elettroforetica delle proteine


Your institution must subscribe to JoVE's Biology section to access this content.

Fill out the form below to receive a free trial or learn more about access:


Enter your email below to get your free 10 minute trial to JoVE!

We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.

If you want more info regarding data storage, please contact



In questo video, si dimostra un metodo per la separazione elettroforetica di proteine ​​mediante poli-acrylimide elettroforesi su gel (PAG).

Cite this Article

Copy Citation | Download Citations

Chakavarti, B., Chakavarti, D. Electrophoretic Separation of Proteins. J. Vis. Exp. (16), e758, doi:10.3791/758 (2008).

Please note that all translations are automatically generated.

Click here for the english version. For other languages click here.


Elettroforesi viene utilizzata per separare miscele complesse di proteine ​​(per esempio, dalle cellule, frazioni subcellulari, frazioni colonna o immunoprecipitati), per indagare composizioni subunità, e per verificare l'omogeneità dei campioni di proteine. Può anche servire a purificare le proteine ​​per l'uso in altre applicazioni. Elettroforesi in gel di poliacrilamide, proteine ​​migrano in risposta ad un campo elettrico attraverso i pori in una matrice di gel di poliacrilammide; dimensione dei pori diminuisce con l'aumentare la concentrazione di acrilamide. La combinazione di dimensione dei pori e la carica di proteine, le dimensioni e la forma determina il tasso di migrazione della proteina. In questa unità, il metodo standard Laemmli è descritto per elettroforesi su gel discontinuo in condizioni di denaturazione, cioè, in presenza di sodio dodecil solfato (SDS).


Il protocollo testo completo di questo approccio sperimentale è disponibile in protocolli di corrente in Biologia Molecolare .

Subscription Required. Please recommend JoVE to your librarian.



  1. Hi nice demo but I did not understand why the presenters kept on showing an agarose gel electrophoresed DNA gel in between as a representation instead of a protein gel.

    Posted by: Anonymous
    June 15, 2008 - 1:11 PM
  2. SYPROorange and SYPROruby staining - two fluorescent-based protein detection methods - can appear like ethidium bromide stained DNA gels.  So those gels you are speaking of may actually be protein gels. 

    Posted by: Anonymous
    June 16, 2008 - 9:34 AM
  3. Yeah possible,  but then why do we see only a single band.I guess SYPRO detection methods would detect the entire protein panel and not specific bands. unless otherwise they are detecting some specific enzyme activity using flourescent detection methods.

    Posted by: Anonymous
    June 16, 2008 - 9:52 AM
  4. We loaded BSA.


    Posted by: Anonymous
    June 17, 2008 - 2:12 AM
  5. Please not that we showed polyacrylamide gel electrophoresis and not agarose gel electrophoresis.


    Bulbul Chakravarti

    Posted by: Anonymous
    June 17, 2008 - 2:08 AM
  6. why cannot i see the video...? pls help me..

    Posted by: Anonymous
    October 10, 2008 - 3:35 AM
  7. i can see it now..thanks...

    Posted by: Anonymous
    October 10, 2008 - 4:50 AM
  8. my network is poor, I can not enjoy the video

    Posted by: Huang C.
    March 15, 2009 - 6:30 AM
  9. Hello, Please shoot me an email at and we'll figure something out for you. Cheers, Nikita

    Posted by: Anonymous
    March 22, 2009 - 10:26 PM
  10. Hi, thanks what can I download your video ? please help me

    Posted by: violet s.
    January 14, 2012 - 10:35 AM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Usage Statistics