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Rato Epidérmico Neural Stem Cell Crest (EPI-NCSC) Culturas

Article DOI: 10.3791/772-v • 08:30 min • May 9th, 2008
May 9th, 2008 •
1,2, 2
1Institute of Human Genetics and Northeast England Stem Cell Institute, Newcastle University, 2Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin

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Aqui nós mostramos o nosso método para isolar do mouse epidérmicas células da crista neural haste (EPI-NCSC). Técnica envolve a micro-dissecando folículos bigode, isolando o bojo e placeing-lo em cultura de tecidos. EPI-NCSC começam a emigrar a partir de explantes saliência para o substrato dentro de 3-4 dias.

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Neurociência Edição 15 epidérmica de células-tronco da crista neural EPI-NCSC mouse explante primário cultura de células,
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