专访:HIV - 1前病毒DNA切除使用一个演进的重组酶

Biology

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Summary

现有的HIV - 1战略采取行动来抑制病毒的生命周期,但不能有效地消除感染。在这里,我们证明了一个精心设计的重组可以有效地消费综合HIV - 1前病毒从感染细胞的基因组DNA。

Cite this Article

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Hauber, J. Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase. J. Vis. Exp. (16), e793, doi:10.3791/793 (2008).

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Abstract

HIV - 1感染细胞整合到宿主染色体和一个由两侧的长末端重复序列的前病毒仍然存在。目前的治疗策略主要目标没有消除感染病毒酶或病毒细胞融合,抑制病毒的生命周期。由于整合前病毒是不通过这些方法,新的耐药菌株的HIV - 1的目标,可能会出现。在这里,我们报告,工程重组TRE(TRE重组,巴克霍尔兹,楼,细胞生物学和遗传学的马克斯普朗克研究所,德累斯顿的分子进化)有效地切除感染细胞的基因组整合的HIV - 1前病毒DNA。 TRE重组是否可以消费的前病毒HIV - 1感染人体细胞的基因组,我们生产的loxLTR含有伪病毒感染的HeLa细胞研究。病毒粒子释放细胞株克隆转染与表达质粒TRE或家长控制向量。重组酶的活性和病毒的生产进行了监测。所有实验证明,无可见的细胞毒作用的前病毒从被感染的细胞的有效删除。这些结果作为证据原则,有可能进化出专门针对HIV - 1 LTR的重组,这种重组是能够切除的HIV - 1前病毒HIV - 1感染人体细胞的基因组。

工程重组之前可以进入的治疗上,然而,重大的障碍需要克服。其中最关键的问题,是我们面临的,是一种高效,安全的运送到靶细胞,并没有副作用。

Protocol

请查看相关影片题为“网站具体的重组酶(SSR)技术的原理和TRE重组酶的分子进化位点特异性重组和工程站点特异性重组酶的有关信息。

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Comments

10 Comments

  1. Please this must be in clinical trials asap..  

    Reply
    Posted by: Anonymous
    June 24, 2008 - 7:26 PM
  2. Moving faster with recombinase. To tell the truth i don' t trust completely in the results of the research, any kind of research, humans trial included. Kp-1461 has been halted almost at the end of phase ² humans trial.. and now seems that kp-1461 can not work in vitro too.. The koronispharma managers have to pay for this.. A LOT OF MONEY.

    Reply
    Posted by: Anonymous
    June 25, 2008 - 8:56 AM
  3. how can I download a video protocol in my flash disc?  

    Reply
    Posted by: Anonymous
    September 20, 2008 - 4:36 PM
  4. Dear Anonymous, please feel free to contact us at support@jove.com and we could discuss your problem.

    Reply
    Posted by: Anonymous
    October 6, 2008 - 6:34 PM
  5. How can I get in touch with doctor Joachim Hauber? I am HIV positive, there isn´t a single day ever since I was diagnosed this disease that I don´t feel like my life just ended. I would do anything to get cured.

    Reply
    Posted by: Anonymous
    October 20, 2008 - 1:37 PM
  6. Reply
    Posted by: Anonymous
    November 3, 2008 - 8:41 AM
  7. Interesting presentation on remarkable research. Worthy of further discussion

    Reply
    Posted by: Anonymous
    February 23, 2009 - 1:48 PM
  8. Have you sequenced the host cell genome in the wild type control vs. the HIV-1 infected vs Tre recombinase cells to
    definitively show where the HIV-1 genome is inserting, and that it is removed?

    Reply
    Posted by: Douglas C.
    October 21, 2009 - 9:41 AM
  9. why can't i get an update on this work?? i have looked everywhere. it has gotten so little publicity..i dont get it

    Reply
    Posted by: Anonymous
    June 25, 2010 - 1:04 AM
  10. hi
    i need full text this article
    plz
    tanx

    Reply
    Posted by: Anonymous
    October 10, 2010 - 4:23 PM

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