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From MEFs to Matrigel 2
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From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

Article DOI: 10.3791/831-v • 06:41 min • June 9th, 2008
June 9th, 2008 •
1, 1, 1
1David Geffen School of Medicine, University of California, Los Angeles

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This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.

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MEFs Matrigel HESCs Feeder Cells Passage Grow Human Embryonic Stem Cells
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