Proper Care and Cleaning of the Microscope

Biology
 

Summary

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.

Cite this Article

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Centonze Frohlich, V. Proper Care and Cleaning of the Microscope. J. Vis. Exp. (18), e842, doi:10.3791/842 (2008).

Abstract

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.

Protocol

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

Disclosures

The authors have nothing to disclose.

Comments

7 Comments

  1. Thank you so much. I find this explanation very helpful. I work with microscopes but I am nos specialist in them so I stand corrected of some mistakes I may have done in the past cleaning my microscopes. Thank you very much.

    Reply
    Posted by: Anonymous
    August 24, 2008 - 4:47 PM
  2. Brilliant. Clear, concise and useful. If only everyone who used our microscope were made to watch this first. Thank-you

    Reply
    Posted by: Anonymous
    September 25, 2008 - 7:17 AM
  3. hi can u tell me how many days once is it recommened to clean lens when in use

    Reply
    Posted by: Anonymous
    November 7, 2009 - 12:15 AM
  4. I would bear her lesson in my mind. Thanks.

    Reply
    Posted by: Anonymous
    May 26, 2010 - 2:58 AM
  5. Do you have a reference for the presence of silicon-based fillers in Kimwipes? I looked around the KIMTECH web site and couldn't find much on their composition. Thanks.

    Reply
    Posted by: Anonymous
    September 15, 2011 - 1:52 PM
  6. vry nice.. found helpful..

    Reply
    Posted by: Anonymous
    January 21, 2012 - 2:42 AM
  7. I like this way to clean optics but it is not the same that I use.
    1. Actually some types kimwipes can be used for cleaning optics in case of gentle use. Some "emerald" colored boxes are good enough (I simply examine them under stereo microscope for particle presence), grey or black boxes as well as light green (as in the video) never must be used for optics. Only for surface cleaning. And of course it better to use special optic tissues because they are simply much cheaper than objective.
    2. I always use stereo microscope to do all lens examination and cleaning. And we have rule that no procedures can be done with lens surface with naked eyes. I even do not allow to remove immersion oil from lens surface to anybody except myself. Removal of excess oil is allowed only from metal protection ring.
    3. And long ago we had ordered lot of cleaning kits from Zeiss. In addition to soft brush, solvents and rubber bulb it contains bottle with extra clean wadding. They are expensive but when you had to operate confocal with it is again not so big costs for proper image. And this kits was ordered in very nice wooden boxes

    Reply
    Posted by: SHOLPAN K.
    July 10, 2015 - 2:21 PM

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