In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Published 9/02/2008
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Biology
 

Summary

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

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Cross, M., Powers, M. In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts. J. Vis. Exp. (19), e908, doi:10.3791/908 (2008).

Abstract

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

Protocol

The complete text protocol for this experimental approach is available in Current Protocols in Cellular Biology.

Disclosures

The authors have nothing to disclose.

Comments

2 Comments

  1. is good congratulations for you job, i am mexico

    Reply
    Posted by: Anonymous
    September 3, 2008 - 12:26 AM
  2. How to see references???

    Reply
    Posted by: Anonymous
    September 7, 2008 - 10:08 PM

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