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Imunoprecipitação DNA metilado
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Imunoprecipitação DNA metilado

Article DOI: 10.3791/935-v • 21:25 min • January 2nd, 2009
January 2nd, 2009 •
*1,2, *1,3, 1,3, 5, 5, 1,2,3, 1,3
1Department of Cancer Genetics and Developmental Biology, BC Cancer Research Centre, 2Interdisciplinary Oncology Program, University of British Columbia - UBC, 3Department of Pathology and Laboratory Medicine, University of British Columbia - UBC, 4Photography/Video Production, Multi-Media Services, BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,, University of British Columbia - UBC
* These authors contributed equally

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Este vídeo demonstra o protocolo para imunoprecipitação DNA metilado (MEDIP). MEDIP é um procedimento dois dias que, seletivamente, extratos de fragmentos de DNA metilado partir de uma amostra do DNA genômico utilizando anticorpos com especificidade para 5-metilcitosina (anti-5 mC).

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Biologia Celular Edição 23 a metilação do DNA imunoprecipitação Epigenomics a epigenética metilcitosina MEDIP protocolo 5-metilcitosina de anticorpos anti-5-metilcitosina microarray
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