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Enregistrement Patch Clamp des canaux ioniques exprimés dans les oocytes de Xenopus
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Enregistrement Patch Clamp des canaux ioniques exprimés dans les oocytes de Xenopus

Article DOI: 10.3791/936-v • 08:01 min • October 16th, 2008
October 16th, 2008 •
1, 2, 2
1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Molecular and Cellular Physiology, Stanford University School of Medicine

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Ceci est conçu comme une introduction à l'enregistrement de patch clamp à partir d'ovocytes de Xenopus laevis. Il couvre l'enlèvement membrane vitelline, la formation d'un joint gigaohm (gigaseal), et la conversion en option du patch à la topologie en dehors-out.

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Biologie cellulaire Numéro 20 électrophysiologie Patch Clamp Clamp Tension les ovocytes biophysique Gigaseal canaux ioniques
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