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Supported Planar Bilayers for the Formation of Study of Immunological Synapses and Kinapse
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Supported Planar Bilayers for the Formation of Study of Immunological Synapses and Kinapse

Article DOI: 10.3791/947-v • 12:24 min • September 15th, 2008
September 15th, 2008 •
1, 1
1Helen and Martin S. Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular, New York University - NYU

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Supported planar bilayers are powerful tools that can be used to model the molecular interactions in an immunological synapse. Here, we show methods for anchoring cell adhesion proteins known to modulate synapse formation to the upper leaflet of the lipid bilyer and visualize synapse formation using TIRF microscopy.

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Supported Planar Bilayers Immunological Synapse Kinapse Molecular Interactions Lymphocytes Antigen Presenting Cells Ni2+-chelating Lipids Recombinant Cell Adhesion Proteins MHC Proteins Poly-histidine Tags Glass Surfaces Specialized Chamber Flow-cell TIRF Microscopy Synapse Formation T Cell Locomotion Receptor Sorting Receptor Degradation
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