JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
The O-glycomap of lubricin, a novel mucin responsible for joint lubrication, identified by site-specific glycopeptide analysis.
Mol. Cell Proteomics
PUBLISHED: 09-03-2014
Show Abstract
Hide Abstract
The lubricative, heavily glycosylated mucin-like synovial glycoprotein, lubricin, has previously been observed to contain glycosylation changes related to rheumatoid and osteoarthritis. Thus, a site-specific investigation of the glycosylation of lubricin was undertaken, in order to further understand the pathological mechanisms involved in these diseases. Lubricin contains an STP rich domain composed of imperfect tandem repeats (EPAPTTPK), the target for O-glycosylation. In this study, a liquid chromatography tandem mass spectrometry approach, using both collision induced and electron transfer dissociation fragmentation methods, identified 185 O-glycopeptides within the STP rich domain of human synovial lubricin. This showed that adjacent threonine residues within the central STP rich region could be simultaneously and/or individually glycosylated. In addition to core 1 structures responsible for biolubrication, core 2 O-glycopeptides were also identified, indicating that lubricin glycosylation may have other roles. Investigation of the expression of polypeptide N-acetylgalactosaminyltransferase genes was carried out using cultured primary fibroblast-like synoviocytes, a cell type that expresses lubricin in vivo. This analysis showed high mRNA expression level of uncharacterized polypeptide N-acetylgalactosaminyltransferase 15 and 5 in addition to the ubiquitously expressed polypeptide N-acetylgalactosaminyltransferase 1 and 2 genes. This suggests that there is a unique combination of transferase genes important for the O-glycosylation of lubricin. The site-specific glycopeptide analysis covered 82% of the protein sequence and has showed that lubricin glycosylation displays both micro- and macroheterogeneity. The density of glycosylation was shown to be high: 168 sites of O-glycosylation, predominately sialylated, were identified. These glycosylation sites were focused in the central STP rich region giving the domain a negative charge. The more positively charged lysine and arginine residues in the N- and C-terminal suggest that synovial lubricin exists as an amphoteric molecule. The identification of these unique properties of lubricin may provide insight into the important low friction lubricating functions of lubricin during natural joint movement.
Related JoVE Video
A room of one's own - Being cared for in a hospital with a single-bed room design.
Scand J Caring Sci
PUBLISHED: 03-23-2014
Show Abstract
Hide Abstract
To illuminate patients' experiences of being hospitalised in a hospital with a single-bed room design.
Related JoVE Video
Fms-like tyrosine kinase 3 ligand controls formation of regulatory T cells in autoimmune arthritis.
PLoS ONE
PUBLISHED: 01-21-2013
Show Abstract
Hide Abstract
Fms-like tyrosine kinase 3 ligand (Flt3L) is known as the primary differentiation and survival factor for dendritic cells (DCs). Furthermore, Flt3L is involved in the homeostatic feedback loop between DCs and regulatory T cell (Treg). We have previously shown that Flt3L accumulates in the synovial fluid in rheumatoid arthritis (RA) and that local exposure to Flt3L aggravates arthritis in mice, suggesting a possible involvement in RA pathogenesis. In the present study we investigated the role of Flt3L on DC populations, Tregs as well as inflammatory responses in experimental antigen-induced arthritis. Arthritis was induced in mBSA-immunized mice by local knee injection of mBSA and Flt3L was provided by daily intraperitoneal injections. Flow cytometry analysis of spleen and lymph nodes revealed an increased formation of DCs and subsequently Tregs in mice treated with Flt3L. Flt3L-treatment was also associated with a reduced production of mBSA specific antibodies and reduced levels of the pro-inflammatory cytokines IL-6 and TNF-?. Morphological evaluation of mBSA injected joints revealed reduced joint destruction in Flt3L treated mice. The role of DCs in mBSA arthritis was further challenged in an adoptive transfer experiment. Transfer of DCs in combination with T-cells from mBSA immunized mice, predisposed naïve recipients for arthritis and production of mBSA specific antibodies. We provide experimental evidence that Flt3L has potent immunoregulatory properties. Flt3L facilitates formation of Treg cells and by this mechanism reduces severity of antigen-induced arthritis in mice. We suggest that high systemic levels of Flt3L have potential to modulate autoreactivity and autoimmunity.
Related JoVE Video
Health status and functional profile at admission of nursing home residents in Iceland over 11-year period.
Int J Older People Nurs
PUBLISHED: 08-01-2011
Show Abstract
Hide Abstract
The knowledge over time of the health status and changes in care needs of newly admitted nursing home residents is limited.
Related JoVE Video
Resistin and insulin/insulin-like growth factor signaling in rheumatoid arthritis.
Arthritis Rheum.
PUBLISHED: 07-09-2011
Show Abstract
Hide Abstract
Human resistin has proinflammatory properties that activate NF-?B-dependent pathways, whereas its murine counterpart is associated with insulin resistance. The aim of this study was to examine potential cross-talk between resistin and insulin/insulin-like growth factor (IGF) signaling in rheumatoid arthritis (RA).
Related JoVE Video
Predicting mortality of residents at admission to nursing home: a longitudinal cohort study.
BMC Health Serv Res
PUBLISHED: 04-20-2011
Show Abstract
Hide Abstract
An increasing numbers of deaths occur in nursing homes. Knowledge of the course of development over the years in death rates and predictors of mortality is important for officials responsible for organizing care to be able to ensure that staff is knowledgeable in the areas of care needed. The aim of this study was to investigate the time from residents admission to Icelandic nursing homes to death and the predictive power of demographic variables, health status (health stability, pain, depression and cognitive performance) and functional profile (ADL and social engagement) for 3-year mortality in yearly cohorts from 1996-2006.
Related JoVE Video
Acuity and anxiety from the patients perspective in the emergency department.
J Emerg Nurs
PUBLISHED: 02-22-2011
Show Abstract
Hide Abstract
Knowledge of a patients perception of her medical needs and urgency may make it possible for emergency nurses to communicate the triage decision and make the patient understand the assessment and triage category. The aim of this study was to compare the patients own assessments of their acuity to the triage nurses assessment, as well as describe patient satisfaction and levels of anxiety.
Related JoVE Video
The tumour-associated glycoprotein podoplanin is expressed in fibroblast-like synoviocytes of the hyperplastic synovial lining layer in rheumatoid arthritis.
Arthritis Res. Ther.
PUBLISHED: 01-11-2011
Show Abstract
Hide Abstract
Activated fibroblast-like synoviocytes (FLSs) in rheumatoid arthritis (RA) share many characteristics with tumour cells and are key mediators of synovial tissue transformation and joint destruction. The glycoprotein podoplanin is upregulated in the invasive front of several human cancers and has been associated with epithelial-mesenchymal transition, increased cell migration and tissue invasion. The aim of this study was to investigate whether podoplanin is expressed in areas of synovial transformation in RA and especially in promigratory RA-FLS.
Related JoVE Video
Testing a Swedish version of the Consumer Emergency Care Satisfaction Scale in an emergency department and 2 observation wards.
J Nurs Care Qual
PUBLISHED: 06-03-2010
Show Abstract
Hide Abstract
The aim was to investigate the construct validity and internal consistency of the Swedish version of the Consumer Emergency Care Satisfaction Scale. The sample comprised 157 persons from an emergency department and an observation ward. The results showed satisfactory reliability, and an explorative factor analysis revealed 3 factors: Caring, Teaching, and Clinical Competence. The Swedish version of the scale seems reliable and may be a useful tool for measuring satisfaction in different acute care settings.
Related JoVE Video
Case managers for frail older people: a randomised controlled pilot study.
Scand J Caring Sci
PUBLISHED: 04-23-2010
Show Abstract
Hide Abstract
The aim was to test sampling and explore sample characteristics in a pilot study using a case management intervention for older people with functional dependency and repeated contact with the healthcare services as well as to investigate the effects of the intervention on perceived health and depressed mood after 3 months. The aim was also to explore internal consistency in the life satisfaction index Z, activities of daily living-staircase and Geriatric Depression Scale-20.
Related JoVE Video
Frail older adults experiences of receiving health care and social services.
J Gerontol Nurs
PUBLISHED: 04-22-2010
Show Abstract
Hide Abstract
This study explored frail older adults overall experience of receiving health care and/or social services. Frail older adults with unstable health are dependent on the health care system because of the frequency of their contacts with it. More knowledge is needed about how they experience the health care and social services they receive. Interviews with 14 older adults (mean age = 81) revealed one main category, Having Power or Being Powerless, that incorporated three categories: Autonomous or Without Control in Relation to the Health Care and/or Social Services System, Being Confirmed or Violated in Relation to Caregivers, and Paradoxes in Health Care or Social Services. Each category comprised subcategories reflecting aspects of having power or being powerless. The findings indicate it is important for health care and social services to ensure a high level of interpersonal continuity to empower frail older adults. This may contribute to more relationship-based care and help older adults remain in control of their life situations.
Related JoVE Video
Patient satisfaction with ambulance care services: survey from two districts in southern Sweden.
Int Emerg Nurs
PUBLISHED: 03-15-2010
Show Abstract
Hide Abstract
One of several quality indicators that are relevant to study is patients satisfaction with health care in the ambulance services. Regular measurements of patient satisfaction in this field have not been carried out previously and a validated method of measurement is lacking.
Related JoVE Video
The experience of being next of kin to an older person in the last phase of life.
Palliat Support Care
PUBLISHED: 02-18-2010
Show Abstract
Hide Abstract
The aim of the study was to investigate the experience of being next of kin to an older person in the last phase of life as narrated after the older persons death.
Related JoVE Video
Anxiety as a factor influencing satisfaction with emergency department care: perspectives of accompanying persons.
J Clin Nurs
PUBLISHED: 09-11-2009
Show Abstract
Hide Abstract
To measure levels of anxiety among people accompanying consumers to the emergency department and to explore how anxiety influences satisfaction with care.
Related JoVE Video
Dizzy - why not take a walk? Low level physical activity improves quality of life among elderly with dizziness.
Gerontology
PUBLISHED: 08-25-2009
Show Abstract
Hide Abstract
Dizziness is a very common complaint. The frequency of dizziness increases with age. It affects quality of life negatively for older persons in several ways.
Related JoVE Video
Frail elderly patients in primary care--their medication knowledge and beliefs about prescribed medicines.
Eur. J. Clin. Pharmacol.
PUBLISHED: 04-22-2009
Show Abstract
Hide Abstract
The aim of this study was to describe elderly patients knowledge about and attitudes towards their medicines in Swedish primary care.
Related JoVE Video
Survivin is an essential mediator of arthritis interacting with urokinase signalling.
J. Cell. Mol. Med.
PUBLISHED: 02-27-2009
Show Abstract
Hide Abstract
Proto-oncogene survivin has recently been identified as a prognostic marker distinguishing patients with destructive rheumatoid arthritis (RA). In the present material of 132 RA patients and 82 controls, the levels of survivin correlated to urokinase (uPA) (r= 0.46), a plasminogen activator over-expressed in inflamed joints and known to exhibit potent arthritogenic properties. Here we evaluate the functional relationship between these proteins using primary synovial fibroblasts and leucocytes of RA patients, human monocytic (THP-1) and fibroblast (MRC-5) cell lines. Using inhibitors of intracellular signalling, we show that uPA and survivin share common transduction pathways in synovial fibroblasts being dependent on the activity of tyrosine kinases, phosphatidylinositide 3 kinase and mitogen effector kinase. Moreover, uPA production is significantly reduced in fibroblasts if survivin synthesis has been silenced by siRNA. Importantly, silencing of survivin in fibroblasts prevented their invasive growth in knee joints of severe combined immune deficient mice. Interaction of uPA with receptor up-regulates survivin expression in leucocytes. In turn, survivin is required for the up-regulation of uPA receptor on the cell surface. These findings indicate that survivin is an essential mediator of arthritogenic properties of uPA regulating its synthesis in synovial fibroblasts and uPAR expression in leucocytes. Close correlation between survivin and uPA levels in patients with RA supports the importance of this connection for the pathogenesis of arthritis.
Related JoVE Video
Opposite effects of PDGF-BB and prostaglandin E1 on cell-motility related processes are paralleled by modifications of distinct actin-binding proteins.
Exp. Cell Res.
PUBLISHED: 01-30-2009
Show Abstract
Hide Abstract
Prostaglandin E(1) (PGE(1)) lowers dermal interstitial fluid pressure (IFP) in vivo and inhibits fibroblast-mediated collagen gel contraction in vitro. PDGF-BB, in contrast, stimulates contraction and normalizes IFP lowered as a result of anaphylaxis. Human diploid AG1518 fibroblasts expressed EP2, EP3 and IP prostaglandin receptors. The inhibitory effect of PGE(1) on contraction depended on cAMP. Short-term stimulation with PDGF-BB transiently induced formation of actin-containing membrane and circular ruffles and breakdown of stress fibers. PGE(1) had no effect on stress fibers nor did it modulate the effects of PDGF-BB. PGE(1) alone or in combination with PDGF-BB inhibited initial adhesion and spreading to collagen. PDGF-BB had no effect on adhesion but stimulated cell spreading. Two-dimensional gel electrophoresis and MALDI TOF analyses of SDS/Triton X-100-soluble proteins revealed changes in migration pattern of actin-binding proteins. Interestingly, PDGF-BB and PGE(1) affected both similar and different sets of actin-binding proteins. PDGF-BB and PGE(1) did not trans-modulate their respective effects on actin-binding proteins, cytoskeletal organization or initial adhesion. Our data show that PDGF-BB stimulates actin cytoskeleton dynamics, whereas PGE(1) inhibits processes dependent on cytoskeletal motor functions. We suggest that these different activities may partly explain the contrasting effects of PGE(1) and PDGF-BB on contraction and IFP.
Related JoVE Video
Human synovial lubricin expresses sialyl Lewis x determinant and has L-selectin ligand activity.
J. Biol. Chem.
Show Abstract
Hide Abstract
Lubricin (or proteoglycan 4 (PRG4)) is an abundant mucin-like glycoprotein in synovial fluid (SF) and a major component responsible for joint lubrication. In this study, it was shown that O-linked core 2 oligosaccharides (Gal?1-3(GlcNAc?1-6)GalNAc?1-Thr/Ser) on lubricin isolated from rheumatoid arthritis SF contained both sulfate and fucose residues, and SF lubricin was capable of binding to recombinant L-selectin in a glycosylation-dependent manner. Using resting human polymorphonuclear granulocytes (PMN) from peripheral blood, confocal microscopy showed that lubricin coated circulating PMN and that it partly co-localized with L-selectin expressed by these cells. In agreement with this, activation-induced shedding of L-selectin also mediated decreased lubricin binding to PMN. It was also found that PMN recruited to inflamed synovial area and fluid in rheumatoid arthritis patients kept a coat of lubricin. These observations suggest that lubricin is able to bind to PMN via an L-selectin-dependent and -independent manner and may play a role in PMN-mediated inflammation.
Related JoVE Video
Quality of care in Icelandic nursing homes measured with Minimum Data Set quality indicators: retrospective analysis of nursing home data over 7 years.
Int J Nurs Stud
Show Abstract
Hide Abstract
The increasing need for long-term care as well as diminished financial resources may compromise the quality of care of older people. Thus the need for clinically based quality of care monitoring to guide development of long-term services has been pointed out.
Related JoVE Video
Thresholds for minimum data set quality indicators developed and applied in icelandic nursing homes.
J Nurs Care Qual
Show Abstract
Hide Abstract
A modified Delphi method was used to determine thresholds for Minimum Data Set quality indicators for Icelandic nursing homes. The thresholds were then applied to quality outcomes in Icelandic nursing homes for the year 2009. The thresholds indicate areas of good or poor care and can be used for planning services. Icelandic nursing homes seem to be doing best in incontinence and nutritional care. However, improvement is needed in care practices for depression, medication, and activity.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.