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Find video protocols related to scientific articles indexed in Pubmed.
[Optimization of fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 10-28-2014
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To optimize the fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana.
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[Two-micron laser resection of the prostate-tangerine technique for the treatment of large-volume prostate].
Zhonghua Nan Ke Xue
PUBLISHED: 10-14-2014
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To evaluate the clinical efficiency and safety of two-micron laser resection of the prostate-tangerine technique (TmLRP-TT) for the treatment of large-volume ( > 70 ml) prostate in patients with benign prostatic hyperplasia (BPH).
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Acacetin-7-rutinoside from Buddleja lindleyana, a new molluscicidal agent against Oncomelania hupensis.
Z. Naturforsch., C, J. Biosci.
PUBLISHED: 07-30-2014
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Buddleja lindleyana is a medicinally important member of the family Loganiaceae distributed in Eastern China. The plant has been used in different traditional medications for the treatment of various diseases. Acacetin-7-rutinoside was isolated from the n-butanol fraction of fresh B. lindleyana leaves and found to be a new molluscicidal agent against the snail Oncomelania hupensis. The structure of acacetin-7-rutinoside was elucidated based on spectral data, including 1H NMR and 13C NMR.
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[Good agricultural practice (GAP) of Chinese materia medica (CMM) for ten years: achievements, problems and proposals].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 07-12-2014
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This paper aims to summarize the achievements during the implementation process of good agricultural practice (GAP) in Chinese Materia Medica (CMM), and on basis of analyzing the existing problems of GAP, to propose further implementation of GAP in TCM growing. Since the launch of GAP in CMM growing ten years ago, it has acquired great achievements, including: (1) The promulgation of a series of measures for the administration of the GAP approval in the CMM growing; (2) The expanded planting area of CMM; (3) The increased awareness of standardized CMM growing among farmers and enterprises; (4) The establishment of GAP implementation bases for CMM growing; (5) The improvement of theory and methodology for CMM growing; (6) The development of a large group of experts and scholars in GAP approval for CMM production. The problems existing in the production include: (1) A deep understanding of GAP and its certification is still needed; (2) The distribution of the certification base is not reasonable; (3) The geo-economics effect and the backward farming practices are thought to be the bottlenecks in the standardization of CMM growing and the scale production of CMM; (4) Low comparative effectiveness limits the development of the GAP; (5) The base of breeding improved variety is blank; (6) The immature of the cultivation technique lead to the risk of production process; (7) The degradation of soil microbial and the continuous cropping obstacle restrict the sustainable development of the GAP base. To further promote the health and orderly GAP in the CMM growing, the authors propose: (1) To change the mode of production; (2) To establish a sound standard system so as to ensure quality products for fair prices; (3) To fully consider the geo-economic culture and vigorously promote the definite cultivating of traditional Chinese medicinal materials; (4) To strengthen the transformation and generalization of basic researches and achievements, in order to provide technical support for the CMM production; (5) To deepen the understanding of GAP, to vigorously promote ecological planting and precision agriculture, in order to overcome the continuous cropping obstacle. The authors think that despite the fact that we are still facing with a huge array of management and technological problems, the GAP in the CMM growing has already enjoyed widespread support and showed great potential. In the future, with people's deeper understanding of GAP and the great progress of the science and technology, the GAP will constantly be fused with the theory, methodology and technology in the modern agriculture like precision agriculture, eco-agriculture and etc.
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Expression of Wnt5a during development of anorectal malformations in a rat model of prenatal exposure to di(n-butyl) phthalate.
Toxicol. Mech. Methods
PUBLISHED: 06-26-2014
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Mounting evidence has indicated the crucial role of Wnt5a in the embryonic development including guts. However, the Wnt5a involvement in the process of anorectal malformations (ARMs) remains unclear. In this study, we examined the expression of Wnt5a during ARMs development in the offspring of di(n-butyl) phthalate (DBP)-treated pregnant rats. During the neonatal period, Wnt5a expression was evaluated in the terminal rectum of ARM offspring, non-ARM littermates and controls. Using real-time polymerase chain reaction (real-time PCR), western-blot analysis and immunohistochemistry approaches, we found a significant decrease of Wnt5a expression in DBP-induced ARMs rats. Collectively, our results demonstrate the aberrant expression of Wnt5a during anorectal development, which suggests that Wnt5a might be involved in DBP-induced ARMs.
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Redundant prepuce increases the odds of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).
Asian J. Androl.
PUBLISHED: 05-31-2014
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Some published evidence has revealed that the dendritic cells can interact with pathogens that exist in the inner foreskin. This information provides a new vision that pathogens could play a role through the redundant prepuce; numerous studies have failed to find pathogens in prostates of patients who had chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). However, no studies have reported an association between foreskin length and CP/CPPS. Hence, we conducted a retrospective case-control study of clinical data from 322 CP/CPPS patients (case group) and 341 nonCP/CPPS patients (control group). Demographic characteristics, lifestyle factors, and foreskin lengths were collected and analyzed. Multivariate logistic regression was adopted to calculate the odds of foreskin length for CP/CPPS. According to the multivariate logistic regression results, when the foreskin length covered up more than half of the glans penis, the odds for CP/CPPS were higher with an increased foreskin (odds ratio (OR): 1.66, 95% confidence interval (CI): 1.04-2.66). In comparison, when the glans penis was completely covered by the foreskin, the OR value increased to 1.86 (95% CI, 1.2-2.88). The study results showed an association between foreskin length and the odds of CP/CPPS. When the foreskin length covered up more than half of the glans penis, there were greater odds for CP/CPPS. This possible mechanism might result from interaction between pathogens and DCs in the inner foreskin, consequently activating T-cells to mediate allergic inflammation in the prostate and producing the autoimmunizations causing CP/CPPS.
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Two-micrometer thulium laser resection of the prostate-tangerine technique for patients with acute urinary retention.
Lasers Med Sci
PUBLISHED: 09-02-2013
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Two-micrometer thulium laser resection of the prostate-tangerine technique (TmLRP-TT) has been introduced as a minimally invasive treatment for benign prostatic hyperplasia (BPH). Acute urinary retention (AUR) is a common and serious complication of BPH. The study was undertaken to assess the clinical efficacy and safety of TmLRP-TT in the treatment of patients with AUR secondary to BPH. A prospective evaluation of 52 patients undergoing TmLRP-TT from December 2011 to November 2012 was carried out. Preoperative status, surgical details, and perioperative complications were recorded. The follow-up outcome was evaluated with subjective and objective tests at 1 and 6 months. Mean age was 70.3?±?7.8 years old. Mean prostate volume was 69.6?±?31.6 ml, and mean residual volume with retention was 274.5?±?150.7 ml. Mean operative time was 64.1?±?30.4 min. Mean catheterization duration was 5.4?±?1.1 days. The mean International Prostate Symptom Score, quality of life score, and postvoid residual urine volume decreased significantly at 6-month follow-up (21.6?±?6.8 vs. 6.8?±?3.2, 4.4?±?1.2 vs. 0.9?±?0.8, 274.5?±?150.7 vs. 40.6?±?22.5 ml). The mean maximum urinary flow rate was 18.7?±?6.9 ml/s postoperative. Two (3.8 %) of the patients required blood transfusion in operation. Five (9.6 %) of the patients had transient hematuria postoperative, and two (3.8 %) of them received 3 days recatheterization due to clot retention. The early clinical results suggest that the TmLRP-TT is a promising safe, effective, and minimally invasive treatment for patients with AUR secondary to BPH. The incidence of complications was low.
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Androgen receptors expressed by prostatic stromal cells obtained from younger versus older males exhibit opposite roles in prostate cancer progression.
Asian J. Androl.
PUBLISHED: 01-09-2013
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Aging is a major risk factor for prostate cancer (PCa), and prostatic stromal cells may also promote PCa progression. Accordingly, stromal cells do not equally promote PCa in older males and younger males. Therefore, it is also possible that the expression of androgen receptors (ARs) by prostatic stromal cells in older versus younger males plays different roles in PCa progression. Using a gene knockdown technique and coculture system, we found that the knockdown of the AR in prostatic stromal cells obtained from younger males could promote the invasiveness and metastasis of cocultured PC3/LNCaP cells in vitro. By contrast, the invasiveness and metastasis of LNCaP cells was inhibited when cocultured with prostatic stromal cells from older males that when AR expression was knocked down. Moreover, after targeting AR expression with small hairpin RNA (shRNA), matrix metalloproteinase (MMP) expression in stromal cells was observed to increase in the younger group, but decreased or remained unchanged in the older group. One exception, however, was observed with MMP9. In vivo, after knocking down AR expression in prostatic stromal cells, the incidence of metastatic lymph nodes was observed to increase in the younger age group, but decreased in the older age group. Together, these data suggest that the AR in prostatic stromal cells played opposite roles in PCa metastasis for older versus younger males. Therefore, collectively, the function of the AR in prostatic stromal cells appears to change with age, and this may account for the increased incidence of PCa in older males.
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[Molluscicidal effect of Phytolacca americana linn leaf against Oncomelania hupensis and its acute toxicity].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 12-15-2011
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To study the molluscicidal activity, the influence on glycogen content of Oncomelania hupensis and the acute toxicity to zebra fish of the extract from Phytolacca americana Linn leaf.
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[Relationship between fractional esterification rate of high density lipoprotein cholesterol and coronary artery disease].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 07-26-2011
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To assess the relationship between fractional esterification rate of high density lipoprotein cholesterol (FER(HDL)) and coronary artery disease.
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The differential effects of prostate stromal cells derived from different zones on prostate cancer epithelial cells under the action of sex hormones.
Asian J. Androl.
PUBLISHED: 07-18-2011
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It is well known that prostate cancer (PCa) occurs predominantly in the peripheral zone (PZ), whereas benign prostatic hyperplasia (BPH) typically develops in the transition zone. To identify possible mechanisms underlying zonal differences, we compared the effects of prostate stromal cells derived from the peripheral zone (PZsc) and the transition zone (TZsc) on a PCa epithelial cell line (PC3) in the presence of sex hormones. First, we observed that androgen receptor (AR) mRNA was more highly expressed in PZsc than TZsc when the cells were treated with dihydrotestosterone (DHT) and ?-oestradiol (E2) (P<0.05). By ELISA, we looked for differences in the secretion of peptide growth factors from PZsc and TZsc. We found that keratinocyte growth factor (KGF) secretion increased with increasing concentrations of DHT (P<0.01) and was higher in PZsc than TZsc. Under treatment with DHT plus E2, PZsc secreted more transforming growth factor-?1 (TGF-?1) than TZsc, but this pattern was reversed when the cells were treated with E2 only. With increasing concentrations of DHT, insulin-like growth factor-1 (IGF-1) secretion increased in PZsc but decreased in TZsc. To further characterize the effects of PZsc and TZsc on PC3 cells, we developed a coculture model and performed MTT assays, Western blot analysis and real-time RT-PCR. We found that PZsc promoted PC3 cell proliferation and progression better than TZsc, particularly when treated with 10 nmol l(-1) DHT plus 10 nmol l(-1) E2. In conclusion, our data suggest that PZsc may have a greater capacity to induce PCa development and progression than TZsc via growth factors regulated by sex hormones. These findings provide possible mechanisms underlying zonal differences in prostate diseases, which may aid the search for novel therapeutic targets for PCa.
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Primary stromal cells isolated from human various histological/pathological prostate have different phenotypes and tumor promotion role.
Chin. Med. J.
PUBLISHED: 07-12-2011
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Prostate stromal cells are known to regulate epithelial growth as well as support and maintain epithelial function. However, how stromal cells regulate epithelial cells and what differences among various histological/pathological prostate stromal cells in prostate cancer progression still remain unclear. This study aimed to investigate the different phenotypes of human various histological/pathological prostate stromal cells, and their role in tumor promotion.
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Differences in phenotype and gene expression of prostate stromal cells from patients of varying ages and their influence on tumour formation by prostate epithelial cells.
Asian J. Androl.
PUBLISHED: 06-06-2011
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Prostate cancer (PCa) is an age-related disease, and the stromal microenvironment plays an important role in prostatic malignant progression. However, the differences in prostate stromal cells present in young and old tissue are still obscure. We established primary cultured stromal cells from normal prostatic peripheral zone (PZ) of donors of varying ages and found that cultured stromal cells from old donors (PZ-old) were more enlarged and polygonal than those from young donors (PZ-young). Furthermore, based on immunocytochemical and ultrastructural analysis, the components of stromal cells changed from a majority of fibroblasts to a mixture of fibroblasts and myofibroblasts with increasing donor age. Using a three-dimensional in vitro culture system, we found that PZ-old stromal cells could enhance the proliferation, migration and invasion of cocultured benign BPH-1 and PC-3 cells. Using an in vivo tissue recombination system, we also found that PZ-old stromal cells are more effective than PZ-young cells in promoting tumour formation by BPH-1 cells of high passage (>100) and PC-3 cells. To probe the possible mechanism of these effects, we performed cDNA microarray analysis and profiled 509 upregulated genes and 188 downregulated genes in PZ-old cells. Among the changed genes, we found genes coding for a subset of paracrine factors that are capable of influencing adjacent epithelial cells; these include hepatocyte growth factor (HGF), fibroblast growth factor 5 (FGF5), insulin-like growth factor 2 (IGF2), insulin-like growth factor-binding protein 4 (IGFBP4), IGFBP5 and matrix metallopeptidase 1 (MMP1). Changes in the expression of these genes were further confirmed by quantitative real-time polymerase chain reaction (PCR), Western blotting and enzyme-linked immunosorbent assays. Overall, our findings indicate that stromal cells from prostate PZ of old donors are more active than similar cells from young donors in promoting the malignant process of adjacent epithelial cells. This finding hints at a new potential strategy for the prevention of PCa.
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[Characterization of stromal cell cultures from the prostatic peripheral zone of men at different ages].
Zhonghua Nan Ke Xue
PUBLISHED: 04-14-2011
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To characterize age-related cellular phenotype alterations and growth rates of human prostatic stromal cell cultures from the normal prostatic peripheral zone of young donors (PZ-young) and old donors (PZ-old).
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[Expression and its relationship of murine double minute 2 and P53 protein in oral leukoplakia and squamous cell carcinoma].
Hua Xi Kou Qiang Yi Xue Za Zhi
PUBLISHED: 03-25-2011
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To study the expression and its relationship of murine double minute 2 (MDM2), P53 protein in oral leukoplakia and squamous cell carcinoma.
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Rapid detection of Rosa laevigata polysaccharide content by near-infrared spectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 02-05-2011
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A method of rapid detecting Rosa laevigata polysaccharide content on the basis of near infrared spectroscopy (NIRS) was established to achieve the purpose of controlling quality of R. laevigata. A total of 129 batches of R. laevigata samples were randomly divided into calibration set and prediction set, number of which were 65 and 64 respectively. The polysaccharide content was measured according to the method provided by Chinese pharmacopoeia, and was 26.05±5.44(%). The Near-infrared diffuse reflectance spectra of R. laevigata were preprocessed by first-order derivative and autoscaling, and was built model with PLS. When 6 Latent variables (LVs) were used in model, the smallest root mean square error of cross validation (RMSECV) was 1.18%, and root mean square error of prediction (RMSEP) was 1.21%. The uninformative variables in spectrum were eliminated by UVE-PLS, and 383 variables were obtained. The prediction accuracy was improved, and RMSECV and RMSEP were 0.90% and 0.99% respectively. Then, 383 variables were further optimized by genetic algorithm (UVE-GA-PLS), and 179 variables were obtained; under this condition, the RMSECV and RMSEP were 0.93% and 1.07% respectively. In this work, result of UVE-PLS was the best. Analyzing variables VIP score in PLS, variable selected by UVE-PLS and UVE-GA-PLS, spectrum region from 7200 cm(-1) to 6700 cm(-1) of the combinations of first and second overtone of C-H and from 5300 cm(-1) to 5000 cm(-1) related to the first overtone of O-H could play an important role in the detection R. laevigata polysaccharide by NIR. Therefore, it was feasible of rapid detection of R. laevigata polysaccharide content by NIRS.
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[The expression of human major histocompatibility complex-I in oral leukoplakia].
Hua Xi Kou Qiang Yi Xue Za Zhi
PUBLISHED: 09-21-2010
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The purpose of this study was to examine the expression of human major histocompatibility complex-I at different degrees of dysplasia leukoplakia, and to investigate local immune status and discuss their associations with oral leukoplakia.
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[Analysis odor of Rhizoma Aatractylodis Macrocephalae based on electronic nose].
Zhong Yao Cai
PUBLISHED: 09-18-2010
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To appraise rationally the quality between wild and cultivated Rhizoma Atractylodis Macrocephalae.
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[Molluscicidal effect of endophyte LL3026 from Buddleia lindleyana against Oncomelania hupensis].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 09-02-2010
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To research the molluscicidal effect, active components, thermal stability and light stability of endophyte LL3026 (Colletotrichum sp.) from Buddleia lindleyana
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Regulatory action of charred Gossamer urocteae on the functions of mouse oral fibroblasts.
J Tradit Chin Med
PUBLISHED: 07-27-2010
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To explore the influence of charred Gossamer urocteae (CGU) on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing.
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[Localization diagnosis of carcinoma in situ of bladder cancer by pirarubicin: preliminary clinical experience].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 07-22-2010
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To investigate whether the location of carcinoma in situ (CIS) of bladder cancer could be macroscopically ascertained by instilling pirarubicin (THP) into urinary bladder.
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Effects of pearl powder extract and its fractions on fibroblast function relevant to wound repair.
Pharm Biol
PUBLISHED: 07-22-2010
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The water soluble matrix (WSM) of pearl powder [Hyriopsis cumingii Lea (Unionidae)] was extracted, and the insoluble residue was demineralized, size-fractionated, and named as MR14 (> 14 kDa), MR3-14 (3-14 kDa), and MR3 (< 3 kDa). The effects of WSM, MR14, MR3-14, and MR3 on primary mouse oral fibroblast proliferation, collagen accumulation, matrix metalloproteinase-2, -9 (MMP-2, -9) activities, and tissue inhibitor of metalloproteinase-1 (TIMP-1) production were tested by MTT assay, chloramine T method, gelatin zymography, and enzyme-linked immunosorbent assay (ELISA), respectively. The results showed that the WSM and MR14 could significantly (p < 0.05) promote fibroblast proliferation; all of the fractions could significantly promote collagen accumulation; MR14 significantly (p < 0.05) inhibited MMP-2 activity; and all of the fractions could significantly promote TIMP-1 production. This study has proved that the mechanism by which pearl powder promotes wound healing is partly due to its ability to stimulate fibroblast mitosis, collagen deposition, and TIMP-1 production, and the major active fraction may be MR14.
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Tetrahydroabietic Acid, a Reduced Abietic Acid, Inhibits the Production of Inflammatory Mediators in RAW264.7 Macrophages Activated with Lipopolysaccharide.
J Clin Biochem Nutr
PUBLISHED: 02-27-2010
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Abietic acid (AA), the main component of the rosin fraction of oleoresin synthesized by conifer species, has been reported to have anti-inflammatory effects. AA is a weak contact allergen; however, compounds resulting from its oxidation by air elicit stronger allergic response. Hydrogenation of the conjugated double bonds of AA, as in tetrahydroabietic acid (THAA), decreases its susceptibility to air oxidation and would thus reduce the allergenicity of AA. The aim of this study was to investigate whether THAA could exert anti-inflammatory effects to the same extent as AA in RAW264.7 macrophages activated with the endotoxin lipopolysaccharide (LPS). THAA and AA inhibited the production of nitric oxide (NO) and prostaglandin E(2) by suppressing the expression of inducible NO synthase and cyclooxygenase-2, respectively, in LPS-activated RAW264.7 macrophages. They also inhibited the LPS-induced production of interleukin (IL)-1beta, IL-6, and tumor necrosis factor-alpha. Both THAA and AA prevented the LPS-induced nuclear translocation of the nuclear factor-kappaB/p65 subunit, suggesting that THAA may inhibit the production of pro-inflammatory mediators through the same mechanism as AA. In comparison, the anti-inflammatory effects of THAA and AA were almost identical, indicating that THAA retains the anti-inflammatory activity of AA at least in LPS-activated RAW264.7 macrophages.
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Androgen receptor is a potential therapeutic target for bladder cancer.
Urology
PUBLISHED: 01-18-2010
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To investigate whether androgen receptor (AR) could serve as a potential molecular target for the treatment of bladder cancer.
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[Targeted degradation of androgen receptors in androgen-independent prostate cancer cells: an experimental study].
Zhonghua Nan Ke Xue
PUBLISHED: 12-09-2009
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To investigate targeted degradation of the androgen receptor (AR) by chimeric molecules (DHT-PROTAC) via the ubiquitin-proteasome pathway in androgen-independent prostate cancer CA-2B cells, and explore the proliferation, secretion and apoptosis of the treated cells.
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Androgen receptor functioned as a suppressor in the prostate cancer cell line PC3 in vitro and in vivo.
Chin. Med. J.
PUBLISHED: 12-03-2009
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Prostate cancer is one of the most common urogenital tumors in the world with an increasing incidence in China. Androgen deprivation therapy is the major therapeutic option for advanced prostate cancer. However, the role of androgen receptor (AR) in hormone-refractory prostate cancer still remains unclear. This work aimed to investigate the role of AR in an androgen independent prostate cancer cell line by in vitro and in vivo studies.
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Small incision combined with laparoscopy for ureteropelvic junction obstruction: comparison with retroperitoneal laparoscopic pyeloplasty.
Chin. Med. J.
PUBLISHED: 12-03-2009
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Laparoscopic dismembered pyeloplasty is technically feasible for ureteropelvic junction (UPJ) obstruction although it is still challenged by its technical difficulty and time-consuming. In this study, we compared the initial results of retroperitoneal laparoscopic pyeloplasty versus a combined laparoscopic dissection and open reconstruction through a small incision in the treatment of UPJ obstruction.
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[Molluscicidal activity of endophyte JJ18 from Pseudolarix kaempferi inoculated to beach soil].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 11-27-2009
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Endophyte JJ18 from Pseudolarix kaempferi was inoculated on solid medium (A), sterile soil (B), and non-sterile soil (C), respectively. The molluscicidal efficacy of fermented products of soil extract was detected according to the immersion test method recommended by WHO, the survival time of JJ18 was observed. Control groups included non-sterile soil (D), ionic water without chloride ion (E) and niclosamide (F). The acute toxicity of A-D group against Artemia salina was determined with artificial seawater as control. After immersing for 24 h, 48 h and 72 h, the snail mortality of group B [(36.67+/-7.56)%, (63.33+/-4.65)%, and (90+/-0)%] was significantly higher than those of group D [(30+/-6.87)%, (33.33+/-5.68)%, and (56.67+/-8.55)%] (P<0.05, P<0.01). Endophyte JJ18 survived in soil for at least 30 days. The mortality of Artemia salina in group B [(25.24+/-3.74)%] was significantly lower than that of group A[(57.15+/-8.90)%] (P<0.01).
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Chimeric molecules facilitate the degradation of androgen receptors and repress the growth of LNCaP cells.
Asian J. Androl.
PUBLISHED: 09-02-2009
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Post-translational degradation of protein plays an important role in cell life. We employed chimeric molecules (dihydrotestosterone-based proteolysis-targeting chimeric molecule [DHT-PROTAC]) to facilitate androgen receptor (AR) degradation via the ubiquitin-proteasome pathway (UPP) and to investigate the role of AR in cell proliferation and viability in androgen-sensitive prostate cancer cells. Western blot analysis and immunohistochemistry were applied to analyse AR levels in LNCaP cells after DHT-PROTAC treatment. Cell counting and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cell viability assay were used to evaluate cell proliferation and viability after AR elimination in both LNCaP and PC-3 cells. AR was tagged for elimination via the UPP by DHT-PROTAC, and this could be blocked by proteasome inhibitors. Degradation of AR depended on DHT-PROTAC concentration, and either DHT or an ALAPYIP-(arg)(8) peptide could compete with DHT-PROTAC. Inhibition of cell proliferation and decreased viability were observed in LNCaP cells, but not in PC-3 or 786-O cells after DHT-PROTAC treatment. These data indicate that AR elimination is facilitated via the UPP by DHT-PROTAC, and that the growth of LNCaP cells is repressed after AR degradation.
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Influence of borneol on primary mice oral fibroblasts: a penetration enhancer may be used in oral submucous fibrosis.
J. Oral Pathol. Med.
PUBLISHED: 03-10-2009
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Borneol, a widely used food and cosmetics additive, has analgesic, anti-inflammatory, antibacterial and penetration enhancing effects. We try to use it as a penetration enhancer for a formula which we have used to treat oral submucous fibrosis (OSF). To assess its safety, we investigate its effects on primary mice oral fibroblasts.
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Clinical relevance of heparanase mRNA expression in bladder cancer and its usefulness as a detection marker in voided urine.
Mol Med Rep
PUBLISHED: 03-01-2009
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Heparanase is an endo-?-glucuronidase that can selectively degrade heparan sulfate glycosaminoglycans and that has been shown to play a role in tumor progression, metastasis and tumor angiogenesis. Previous studies have demonstrated that the overexpression of heparanase in human tumors facilitates their invasive activity, thereby enhancing tumor metastatic potential. We detected the simultaneous and significantly correlated overexpression of heparanase in the tissues and urine of patients with bladder cancer. Expression of heparanase mRNA in the tissues was significantly correlated with various clinicopathological factors, such as stage, histological grade, size, number, recurrence and lymph node metastasis. This was not the case in the urine. In addition, the relapse rate was significantly higher in the patients exhibiting heparanase mRNA expression than in the patients lacking it. Heparanase at the mRNA level had greater sensitivity in the detection of bladder cancer than cytology, and specificity for the marker was relatively high. These findings suggest that the overexpression of heparanase may influence various malignant behaviors in bladder cancer, and that the RT-PCR assay for heparanase mRNA is a promising non-invasive test with high sensitivity and specificity for the diagnosis of the disease.
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Tumor formation of prostate cancer cells influenced by stromal cells from the transitional or peripheral zones of the normal prostate.
Asian J. Androl.
PUBLISHED: 01-05-2009
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This study was designed to investigate the different involvements of prostatic stromal cells from the normal transitional zone (TZ) or peripheral zone (PZ) in the carcinogenesis of prostate cancer (PCa) epithelial cells (PC-3) in vitro and in vivo co-culture models. Ultra-structures and gene expression profiles of primary cultures of human prostatic stromal cells from the normal TZ or PZ were analyzed by electron microscopy and microarray analysis. In vitro and in vivo co-culture models composed of normal TZ or PZ stromal cells and human PCa PC-3 cells were established. We assessed tumor growth and weight in the in vivo nude mice model. There are morphological and ultra-structural differences in stromal cells from TZ and PZ of the normal prostate. In all, 514 differentially expressed genes were selected by microarray analysis; 483 genes were more highly expressed in stromal cells from TZ and 31 were more highly expressed in those from PZ. Co-culture with PZ stromal cells and transforming growth factor-beta1 (TGF-beta1) increased the tumor growth of PC-3 cells in vitro and in vivo, as well as Bcl-2 expression. On the other hand, stromal cells of TZ suppressed PC-3 cell tumor growth in the mouse model. We conclude that ultra-structures and gene expression differ between the stromal cells from TZ or PZ of the normal prostate, and stroma-epithelium interactions from TZ or PZ might be responsible for the distinct zonal localization of prostate tumor formation.
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[Differential gene expressions in the stromal cells of the human prostate peripheral zone in males of different ages].
Zhonghua Nan Ke Xue
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To study the gene expressions in the stromal cells of the human prostate peripheral zone (PZ) in men of different ages.
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Effects of AIBL on Oncomelania hupensis, the intermediate snail host of Schistosoma japonicum: an enzyme histochemical study.
Asian Pac J Trop Med
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To explore the effect of AIBL on Oncomelania hupensis, the intermediate snail host of Schistosoma japonicum.
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NADPH oxidase 2 plays a critical role in dysfunction and apoptosis of pancreatic ?-cells induced by very low-density lipoprotein.
Mol. Cell. Biochem.
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In type 2 diabetes, pancreatic ?-cells cannot secret enough insulin compensate for insulin resistance, which are often accompanied by abnormality in lipid metabolism such as hypertriglyceridemia. It is reported that oxidative stress is involved in pancreatic ?-cell dysfunction. However, molecular mechanisms linking between excessive generations of reactive oxygen species (ROS) and ?-cell dysfunction and apoptosis induced by high levels of very low-density lipoprotein (VLDL) are poorly understood. In this study, we test the hypothesis that NADPH oxidase 2 (NOX2)-derived ROS may play a key role in dysfunction and apoptosis of pancreatic ?-cell induced by VLDL. Our results show that the ApoCIII transgenic mice displayed increased serum TG levels, enhanced generation of ROS and impaired insulin content in pancreatic ?-cells. In vitro, the treatment of pancreatic NIT-1 cells with 1 mg/ml VLDL for 12 h stimulated NOX2-derived ROS generation, decreased expression and secretion of insulin. Furthermore, we found that VLDL induced dysfunction and apoptosis of pancreatic ?-cells through JNK and p53 pathways, which were rescued by siRNA-mediated NOX2 reduction. In conclusion, our data demonstrate a critical role of NOX2-derived ROS in dysfunction and apoptosis through JNK and p53 pathways in pancreatic ?-cells induced by VLDL.
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NADPH oxidase 2-derived reactive oxygen species are involved in dysfunction and apoptosis of pancreatic ?-cells induced by low density lipoprotein.
Cell. Physiol. Biochem.
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Increased levels of plasma cholesterol are a common feature of patient of type 2 diabetes. However, the links between elevated levels of low-density lipoprotein (LDL) and dysfunction of ?-cells are still unclear.
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Fermentation enhances the in vitro antioxidative effect of onion (Allium cepa) via an increase in quercetin content.
Food Chem. Toxicol.
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Yellow onion (Allium cepa) extract showed enhanced antioxidative effects in 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC) and 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate and acetyl ester (CM-H(2)DCFDA) assay after being treated with a crude enzyme extract from soybean paste fungi, Aspergillus kawachii. HPLC analysis showed two increased and two decreased peaks after enzyme treatment. The decreased peaks were identified as quercetin-3,4-di-O-?-d-glucoside (1) and quercetin-4-O-?-d-glucoside (2), and peaks that increased were quercetin-3-O-?-d-glucoside (3) and quercetin (4), respectively. It was expected that 3 and 4 were originated from the glucosidic cleavage of their glucosides, 1 and 2. Among the increased compounds, only quercetin (4) showed strong antioxidative activity in the DPPH assay. In addition, the protective effect against glutamate-induced neurotoxicity in HT22 cells was increased when treated with 25 ?g/ml of fermented onion. The enhanced neuroprotective effect was also originated from the increased quercetin content. As a consequence, fermentation raised the quercetin content in onion, and subsequently increased the antioxidative and neuroprotective activities.
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[Analysis of N-E cadherin switch as an independent predictive parameter of bladder cancer survival outcomes].
Zhonghua Yi Xue Za Zhi
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To explore the expression patterns of cadherins (N-cadherin and E-cadherin) in urothelial carcinomas and understand the values of N-E cadherin switch in the predictions of postoperative recurrence and prognosis.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.