JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Replication of the 4p16 susceptibility locus in congenital heart disease in Han Chinese populations.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Congenital heart disease (CHD) is the most common form of congenital human birth anomalies and a leading cause of perinatal and infant mortality. Some studies including our published genome-wide association study (GWAS) of CHD have indicated that genetic variants may contribute to the risk of CHD. Recently, Cordell et al. published a GWAS of multiple CHD phenotypes in European Caucasians and identified 3 susceptibility loci (rs870142, rs16835979 and rs6824295) for ostium secundum atrial septal defect (ASD) at chromosome 4p16. However, whether these loci at 4p16 confer the predisposition to CHD in Chinese population is unclear. In the current study, we first analyzed the associations between these 3 single nucleotide polymorphisms (SNPs) at 4p16 and CHD risk by using our existing genome-wide scan data and found all of the 3 SNPs showed significant associations with ASD in the same direction as that observed in Cordell's study, but not with other subtypes- ventricular septal defect (VSD) and ASD combined VSD. As these 3 SNPs were in high linkage disequilibrium (LD) in Chinese population, we selected one SNP with the lowest P value in our GWAS scan (rs16835979) to perform a replication study with additional 1,709 CHD cases with multiple phenotypes and 1,962 controls. The significant association was also observed only within the ASD subgroup, which was heterogeneous from other disease groups. In combined GWAS and replication samples, the minor allele of rs16835979 remained significant association with the risk of ASD (OR = 1.22, 95% CI = 1.08-1.38, P = 0.001). Our findings suggest that susceptibility loci of ASD identified from Cordell's European GWAS are generalizable to Chinese population, and such investigation may provide new insights into the roles of genetic variants in the etiology of different CHD phenotypes.
Related JoVE Video
Highly ordered GaN-based nanowire arrays grown on patterned (100) silicon and their optical properties.
Chem. Commun. (Camb.)
PUBLISHED: 11-27-2013
Show Abstract
Hide Abstract
Arrays of GaN-based nanowires have been synthesized on patterned silicon without a catalyst. The spatial density, length and average radius of the nanowires can be well-controlled. The GaN core contains two semipolar facets and a controllable polar facet. The nanowire heterostructures exhibit excellent laser behavior.
Related JoVE Video
A genome-wide association study identifies two risk loci for congenital heart malformations in Han Chinese populations.
Nat. Genet.
PUBLISHED: 04-12-2013
Show Abstract
Hide Abstract
Congenital heart malformation (CHM) is the most common form of congenital human birth anomaly and is the leading cause of infant mortality. Although some causative genes have been identified, little progress has been made in identifying genes in which low-penetrance susceptibility variants occur in the majority of sporadic CHM cases. To identify common genetic variants associated with sporadic non-syndromic CHM in Han Chinese populations, we performed a multistage genome-wide association study (GWAS) in a total of 4,225 CHM cases and 5,112 non-CHM controls. The GWAS stage included 945 cases and 1,246 controls and was followed by 2-stage validation with 2,160 cases and 3,866 controls. The combined analyses identified significant associations (P < 5.0 × 10??) at 1p12 (rs2474937 near TBX15; odds ratio (OR) = 1.40; P = 8.44 × 10?¹?) and 4q31.1 (rs1531070 in MAML3; OR = 1.40; P = 4.99 × 10?¹²). These results extend current knowledge of genetic contributions to CHM in Han Chinese populations.
Related JoVE Video
Enhanced performance of InGaN/GaN based solar cells with an In(0.05)Ga(0.95)N ultra-thin inserting layer between GaN barrier and In(0.2)Ga(0.8)N well.
Opt Express
PUBLISHED: 04-03-2013
Show Abstract
Hide Abstract
The effect of ultra-thin inserting layer (UIL) on the photovoltaic performances of InGaN/GaN solar cells is investigated. With UIL implemented, the open-circuit voltage was increased from 1.4 V to 1.7 V, short-circuit current density was increased by 65% and external quantum efficiency was increased by 59%, compared to its counterparts at room temperature under 1-sun AM1.5G illumination. The improvements in electrical and photovoltaic properties are mainly attributed to the UIL which can boost the crystal quality and alleviate strain. Moreover, it can act as a transition layer for higher indium incorporation and an effective light sub-absorption layer in multiple quantum wells.
Related JoVE Video
?-Lipoic acid reduces infarct size and preserves cardiac function in rat myocardial ischemia/reperfusion injury through activation of PI3K/Akt/Nrf2 pathway.
PLoS ONE
PUBLISHED: 02-04-2013
Show Abstract
Hide Abstract
The present study investigates the effects and mechanisms of ?-Lipoic acid (LA) on myocardial infarct size, cardiac function and cardiomyocyte apoptosis in rat hearts subjected to in vivo myocardial ischemia/reperfusion (MI/R) injury.
Related JoVE Video
Correction of tetralogy of fallot with combination of operative and interventional methods.
World J Pediatr Congenit Heart Surg
PUBLISHED: 10-01-2011
Show Abstract
Hide Abstract
A one-stage repair was planned for an 11-year-old boy with tetralogy of Fallot. After initial attempts to wean from cardiopulmonary bypass were unsuccessful, an atrial septal defect and a ventricular septal defect were created in order to achieve hemodynamic stability. The boy recovered from the operation but had large volume of chest drainage. Transthoracic echocardiography revealed left to right shunting at both atrial and ventricular levels. Interventional catheter-directed devices were used to repair the residual shunts successfully in the catheterization laboratory.
Related JoVE Video
Totally thoracoscopic surgical resection of cardiac myxoma in 12 patients.
Ann. Thorac. Surg.
PUBLISHED: 07-30-2010
Show Abstract
Hide Abstract
Myxomas are the most common type of primary cardiac tumors. We report our use of totally thoracoscopic surgery in resecting cardiac myxomas in 12 cases with 10 in the left atria and 2 in the right atria. Totally thoracoscopic surgical resection of myxoma was successfully performed in all cases through three minimal incisions, with the largest incision less than 3 cm. The cardiopulmonary bypass time was 96 to 126 minutes, and the aortic cross-clamp time was 46 to 63 minutes. Postoperative ventilation assistance was 3 to 11 hours. We show that the method is safe and achieves complete tumor resection.
Related JoVE Video
Surgical treatment of severe complications caused by transcatheter closure of ventricular septal defects.
World J Pediatr Congenit Heart Surg
PUBLISHED: 07-01-2010
Show Abstract
Hide Abstract
The objective was to report the surgical results following failed transcatheter intervention for closure of ventricular septal defects (VSDs). This study is a retrospective analysis of patients (n = 9) from Xijing Hospital (Xian, China) with failed transcatheter intervention for VSDs who subsequently underwent open heart surgery. Five patients experienced complications during transcatheter intervention, including third-degree atrioventricular block (III° AVB) (n = 2), aortic incompetence (n = 2), or tricuspid incompetence (n = 1). The devices were immediately removed in the catheterization laboratory followed by open heart surgery to repair VSDs. Four patients experienced complications after transcatheter intervention; one patients device was displaced into the right ventricle, and 3 patients had III° AVB. These patients underwent surgery to retrieve the devices and to repair VSDs. All cardiac surgery was performed under general anesthesia and under cardiopulmonary bypass. Postoperatively, all patients recovered uneventfully with no deaths or complications. The patients with III° AVB after device implantation recovered sinus rhythm postoperatively, and tricuspid apparatus injuries were surgically repaired with valvuloplasty. Transcatheter interventional VSD closure is safe and effective, but only under the conditions of strict patient selection, proper technique, and device application. Once severe complications are observed and diagnosed, devices should be retrieved immediately, and open heart surgery should be performed to avoid further injury.
Related JoVE Video
Spontaneous dissection of left anterior descending coronary artery: the diagnostic role of dual-source computed tomography.
J Thorac Imaging
PUBLISHED: 05-14-2010
Show Abstract
Hide Abstract
Spontaneous coronary artery dissection is usually diagnosed by an urgent coronary angiography. We report a case of spontaneous coronary artery dissection of the left anterior descending coronary artery, which was diagnosed by dual-source computed tomography. Subsequent coronary angiography confirmed the result. Computed tomographic coronary angiography can be a complementary diagnostic tool for the assessment of coronary artery dissection.
Related JoVE Video
Enhancing efficacy and mucosa-tropic distribution of an oral HIV-PsV DNA vaccine in animal models.
J Drug Target
PUBLISHED: 10-30-2009
Show Abstract
Hide Abstract
A strategy combined the oral delivery route and bovine papillomavirus (BPV) pseudovirus (PsV)-based human immunodeficiency virus (HIV) DNA vaccine, which has been proven to enhance the mucosal immunization compared with the systemic immunization and in general does not induce effective mucosal immune responses. In this study, the immune responses against the BPV expressing HIV gp41 epitopes (ELDKWA, NWFDIT) after oral administration in Cynomolgus monkeys (Macaca fascicularis) were assessed, and the biodistribution of plasmid DNA encapsulated in the papillomavirus-like particles (VLPs) were evaluated in murine models. Results showed that oral immunization with the HIV-PsV DNA vaccine in monkey generated p24 and gp41 epitopes-specific serum IgG. Importantly, these induced antibodies had been shown to neutralize HIV-1 primary strain. In addition, the advantage of VLPs as vehicles delivering genes had been first revealed in biodistribution results. Therefore, orally administered HIV-PsV DNA vaccine was well-tolerated, enhanced the mucosa targeting property of the plasmid DNA, and reduced the nontargeting distribution, which indicate that it would reduce stress associated with systemic vaccination.
Related JoVE Video
Carboxy terminus of heat shock protein (HSP) 70-interacting protein (CHIP) inhibits HSP70 in the heart.
J. Physiol. Biochem.
Show Abstract
Hide Abstract
Heat shock protein (HSP) 70 plays a critical role in protecting the heart from various stressor-induced cell injuries; the mechanism remains to be further understood. The present study aims to elucidate the effect of a probiotics-derived protein, LGG-derived protein p75 (LGP), in alleviating the ischemia/reperfusion (I/R)-induced heart injury. We treated rats with the I/R with or without preadministration with LGP. The levels of HSP70 and carboxy terminus of HSP70-interacting protein (CHIP) in the heart tissue were assessed by enzyme-linked immunosorbent assay (ELISA) and Western blotting. The effect of CHIP on suppression of HSP70 and the effect of LGP on suppression of CHIP were investigated with an I/R rat model and a cell culture model. The results showed that I/R-induced infarction in the heart could be alleviated by pretreatment with LGP. HSP70 was detected in naïve rat heart tissue extracts. I/R treatment significantly suppressed the level of HSP70 and increased the levels of CHIP in the heart. A complex of CHIP/HSP70 was detected in heart tissue extracts. The addition of recombinant CHIP to culture inhibited HSP70 in heart cells. LGP was bound CHIP in heart cells and prevented the CHIP from binding HSP70. In summary, I/R can suppress HSP70 and increase CHIP in heart cells. CHIP can suppress HSP70 that can be prevented by pretreatment with LGP. The results imply that CHIP may be a potential target in the prevention of I/R-induced heart cell injury.
Related JoVE Video
Heparanase DNA vaccine delivered by electroporation induces humoral immunity and cytoimmunity in animal models.
Vaccine
Show Abstract
Hide Abstract
Electroporation (EP)-assisted DNA vaccination has been proven effective as an approach to the treatment of cancer. Although heparanase (HPA) is a potential target for patients with advanced tumor diseases, the efficacy of immunotherapeutic strategies targeting HPA has never been evaluated. In this study, humoral immunity was elicited using genetic vaccinations between C57BL/6J mice and Macaca fascicularis. The immunized serum neutralized HPA activity and attenuated the invasion of B16 cells in vitro. In addition, T lymphocytes from the splenic cells of the immunized mice induced HPA-specific cytotoxic lymphocytes (CTLs), which verified cytoimmunity. Prophylactic vaccination significantly suppressed tumor growth and metastasis in vivo and prolonged the survival rate in tumor-bearing murine models. In addition, RT-PCR and Western blot analyses of the primary tumors indicated less proliferation and angiogenesis and more apoptosis in the HPA-immunization immunotherapy groups. Simultaneously, the levels of IL-2, IL-4, and IFN-? were not significantly greater in the HPA-immunized group than in PBS controls. Thus, we conclude that the combination of an anti-HPA antibody and a CTL response in HPA-immunization gene therapy is enough to attenuate tumor growth and metastasis. This is the first time that a DNA vaccine targeting HPA immunization assisted by EP has induced humoral immunity and cytoimmunity in vivo. This provides a basis for the continued development of DNA vaccines targeting HPA and the use of such vaccines in clinical settings.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.