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Find video protocols related to scientific articles indexed in Pubmed.
De Novo Chemoattractants Form Supramolecular Hydrogels for Immunomodulating Neutrophils In Vivo.
Bioconjug. Chem.
PUBLISHED: 11-15-2014
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Most of immunomodulatory materials (e.g., vaccine adjuvants such as alum) modulate adap-tive immunity, and yet little effort has focused on developing materials to regulate innate im-munity, which get mentioned only when inflammation affects the biocompatibility of bio-materials. Traditionally considered as short-lived effector cells from innate immunity primarily for the clearance of invading microorganisms without specificity, neutrophils exhibit key role in launching and shaping the immune response. Here we show that the incorporation of un-natural amino acids into a well-known chemoattractant-N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLF)-offers a facile approach to create a de novo, multifunctional chemoat-tractant that self-assembles to form supramolecular nanofibrils and hydrogels. This de novo chemoattractant not only exhibits preserved cross-species chemoattractant activity to human and murine neutrophils, but also effectively resists proteolysis. Thus, its hydrogel, in vivo, re-leases the chemoattractant and attracts neutrophils to the desired location in a sustainable manner. As a novel and general approach to generate a new class of biomaterials for modu-lating innate immunity, this work offers a prolonged acute inflammation model for developing various new applications.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.