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Find video protocols related to scientific articles indexed in Pubmed.
[In vitro susceptibilities to levofloxacin and various antibacterial agents of 12,919 clinical isolates obtained from 72 centers in 2007].
Keizo Yamaguchi, Akira Ohno, Yoshikazu Ishii, Kazuhiro Tateda, Morihiro Iwata, Makoto Kanda, Kouji Akizawa, Chikara Shimizu, Shinichirou Kon, Kastushi Nakamura, Keiko Matsuda, Makoto Tominaga, Takuo Nakagawa, Akihiro Sugita, Tatsumi Ito, Jun Kato, Akira Suwabe, Kumiko Yamahata, Chizuko Kawamura, Hiromi Tashiro, Hiroko Horiuchi, Yosei Katayama, Shigemi Kondou, Shigeki Misawa, Misturu Murata, Yoshio Kobayashi, Hideyuki Okamoto, Kenichiro Yamazaki, Motoi Okada, Kosuke Haruki, Harushige Kanno, Masanori Aihara, Shigefumi Maesaki, Giichi Hashikita, Eiji Miyajima, Midori Sumitomo, Takefumi Saito, Nobuo Yamane, Chieko Kawashima, Takahisa Akiyama, Tamio Ieiri, Yoshitaka Yamamoto, Yuki Okamoto, Hidetoshi Okabe, Kunihiko Moro, Masayo Shigeta, Haruyoshi Yoshida, Masanobu Yamashita, Yukio Hida, Takayuki Takubo, Tadashi Kusakabe, Hiroya Masaki, Hitoshi Heijyou, Hideo Nakaya, Kunimitsu Kawahara, Reiko Sano, Syuji Matsuo, Hisashi Kono, Yosuke Yuzuki, Norio Ikeda, Masayo Idomuki, Masayuki Soma, Go Yamamoto, Syohiro Kinoshita, Seiji Kawano, Mikio Oka, Nobuchika Kusano, Dongchon Kang, Junko Ono, Minoru Yasujima, Makoto Miki, Masato Hayashi, Syunji Okubo, Syunkou Toyoshima, Mitsuo Kaku, Imao Sekine, Joji Shiotani, Hajime Horiuchi, Yoko Tazawa, Akiko Yoneyama, Kazunari Kumasaka, Kazuhiko Koike, Nobuyuki Taniguchi, Yukio Ozaki, Takashi Uchida, Masami Murakami, Kazuhisa Inuzuka, Hideo Gonda, Ikuo Yamaguchi, Yoshinori Fujimoto, Junji Iriyama, Yuko Asano, Hitoshi Genma, Masato Maekawa, Hitoshi Yoshimura, Kaname Nakatani, Hisashi Baba, Satoshi Ichiyama, Shinichi Fujita, Masao Kuwabara, Toshiro Okazaki, Hiromitsu Fujiwara, Hiromi Ota, Astushi Nagai, Jun Fujita, Kiyoshi Negayama, Tetsuro Sugiura, Mikio Kamioka, Mitsuharu Murase, Nobuhisa Yamane, Isamu Nakasone, Akihiko Okayama, Yosuke Aoki, Koji Kusaba, Yukari Nakashima, Hiroaki Miyanohara, Kazufumi Hiramatsu, Tetsunori Saikawa, Katsunori Yanagihara, Junichi Matsuda, Shigeru Kohno, Koichi Mashiba.
Jpn J Antibiot
PUBLISHED: 10-29-2009
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We have reported in this journal in vitro susceptibilities of clinical isolates to antibiotics every year since 1992. In this paper, we report the results of an analysis of in vitro susceptibilities of 12,919 clinical isolates from 72 centers in Japan to selected antibiotics in 2007 compared with the results from previous years. The common respiratory pathogens, Streptococcus pyogenes, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae maintained a high susceptibility to fluoroquinolones (FQs). The resistance of S. pyogenes to macrolides has been increasing every year and this was especially clear this year. Most strains of Enterobacteriaceae except for Escherichia coli showed a high susceptibility to FQs. Almost 30% of E. coli strains were resistant to FQs and the resistance increased further this year. FQs resistance of methicillin-resistant Staphylococcus aureus (MRSA) was approximately 95% with the exception of 45% for sitafloxacin (STFX). FQs resistance of methicillin-susceptible S. aureus (MSSA) was low at about 10%. FQs resistance of methicillin-resistant coagulase negative Staphylococci (MRCNS) was higher than that of methicillin-susceptible coagulase negative Staphylococci (MSCNS), but it was lower than that of MRSA. However, FQs resistance of MSCNS was higher than that of MSSA. FQs resistance of Enterococcus faecalis was 22.5% to 29.6%, while that of Enterococcusfaecium was more than 85% except for STFX (58.3%). In clinical isolates of Pseudomonas aeruginosa derived from urinary tract infections, FQs resistance was 21-27%, which was higher than that of P. aeruginosa from respiratory tract infections at 13-21%, which was the same trend as in past years. Multidrug resistant strains accounted for 5.6% in the urinary tract and 1.8% in the respiratory tract. Acinetobacter spp. showed high susceptibility to FQs. The carbapenem resistant strains, which present a problem at present, accounted for 2.7%. Neisseria gonorrhoeae showed high resistance of 86-88% to FQs. The results of the present survey indicated that although methicillin-resistant Staphylococci, Enterococci, E. coli, P. aeruginosa, and N. gonorrhoeae showed resistance tendencies, and other species maintained high susceptibility rates more than 90% against FQs, which have been used clinically for over 15 years.
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Mycobacterium kyorinense sp. nov., a novel, slow-growing species, related to Mycobacterium celatum, isolated from human clinical specimens.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 06-09-2009
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A novel, non-pigmented, slow-growing mycobacterium was identified on the basis of biochemical and nucleic acid analyses, as well as growth characteristics. Three isolates were cultured from clinical samples (two from sputum and one from pus in lymph nodes) obtained from three immunocompetent patients with infections. Bacterial growth occurred at 28-42 degrees C on Middlebrook 7H11-OADC agar. The isolates showed negative results for Tween hydrolysis, nitrate reductase, semiquantitative catalase, urease activity, 3 day arylsulfatase activity, pyrazinamidase, tellurite reduction and niacin accumulation tests, but positive results for 14 day arylsulfatase activity and heat-stable catalase tests. The isolates contained alpha-, keto-, and dicarboxymycolates in their cell walls. Sequence analysis revealed that all isolates had identical, unique 16S rRNA sequences. Phylogenetic analysis of the 16S rRNA, rpoB, hsp65 and sodA gene sequences confirmed that these isolates are unique but closely related to Mycobacterium celatum. DNA-DNA hybridization of the isolates demonstrated less than 50 % reassociation with M. celatum and Mycobacterium branderi. On the basis of these findings, a novel species designated Mycobacterium kyorinense sp. nov. is proposed. The type strain is KUM 060204(T) (=JCM 15038(T)=DSM 45166(T)).
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.