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Find video protocols related to scientific articles indexed in Pubmed.
H-CRRETAWAC-OH, a Lead Structure for the Development of Radiotracer Targeting Integrin ? 5 ? 1 ?
Biomed Res Int
PUBLISHED: 04-25-2014
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Imaging of angiogenic processes is of great interest in preclinical research as well as in clinical settings. The most commonly addressed target structure for imaging angiogenesis is the integrin ? v ? 3. Here we describe the synthesis and evaluation of [(18)F]FProp-Cys(*)-Arg-Arg-Glu-Thr-Ala-Trp-Ala-Cys(*)-OH, a radiolabelled peptide designed to selectively target the integrin ? 5 ? 1. Conjugation of 4-nitrophenyl-(RS)-2-[(18)F]fluoropropionate provided [(18)F]FProp-Cys(*)-Arg-Arg-Glu-Thr-Ala-Trp-Ala-Cys(*)-OH in high radiochemical purity (>95%) and a radiochemical yield of approx. 55%. In vitro evaluation showed ? 5 ? 1 binding affinity in the nanomolar range, whereas affinity to ? v ? 3 and ? IIb ? 3 was >50??M. Cell uptake studies using human melanoma M21 (? v ? 3-positive and? 5 ? 1-negative), human melanoma M21-L (? v ? 3-negative and ? 5 ? 1-negative), and human prostate carcinoma DU145 (? v ? 3-negative and ? 5 ? 1-positive) confirmed receptor-specific binding. The radiotracer was stable in human serum and showed low protein binding. Biodistribution studies showed tumour uptake ranging from 2.5 to 3.5% ID/g between 30 and 120?min post-injection. However, blocking studies and studies using mice bearing ? 5 ? 1-negative M21 tumours did not confirm receptor-specific uptake of [(18)F]FProp-Cys(*)-Arg-Arg-Glu-Thr-Ala-Trp-Ala-Cys(*)-OH, although this radiopeptide revealed high affinity and substantial selectivity to ? 5 ? 1 in vitro. Further experiments are needed to study the in vivo metabolism of this peptide and to develop improved radiopeptide candidates suitable for PET imaging of ? 5 ? 1 expression in vivo.
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Design and evaluation of novel radiolabelled VIP derivatives for tumour targeting.
Anticancer Res.
PUBLISHED: 04-09-2013
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Vasoactive intestinal peptide (VIP) receptors are overexpressed in a broad variety of tumours. For the detection of these tumours, novel chemically modified and shortened VIP derivatives were designed.
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Development of ??Ga-labelled DTPA galactosyl human serum albumin for liver function imaging.
Eur. J. Nucl. Med. Mol. Imaging
PUBLISHED: 01-24-2013
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The hepatic asialoglycoprotein receptor is responsible for degradation of desialylated glycoproteins through receptor-mediated endocytosis. It has been shown that imaging of the receptor density using [(99m)Tc]diethylenetriamine pentaacetic acid (DTPA) galactosyl human serum albumin ([(99m)Tc]GSA) allows non-invasive determination of functional hepatocellular mass. Here we present the synthesis and evaluation of [(68)Ga]GSA for the potential use with positron emission tomography (PET).
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Tumor targeting and imaging with dual-peptide conjugated multifunctional liposomal nanoparticles.
Int J Nanomedicine
PUBLISHED: 01-01-2013
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The significant progress in nanotechnology provides a wide spectrum of nanosized material for various applications, including tumor targeting and molecular imaging. The aim of this study was to evaluate multifunctional liposomal nanoparticles for targeting approaches and detection of tumors using different imaging modalities. The concept of dual-targeting was tested in vitro and in vivo using liposomes derivatized with an arginine-glycine-aspartic acid (RGD) peptide binding to ?v?3 integrin receptors and a substance P peptide binding to neurokinin-1 receptors.
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Comparison of biological stability and metabolism of CCK2 receptor targeting peptides, a collaborative project under COST BM0607.
Eur. J. Nucl. Med. Mol. Imaging
PUBLISHED: 04-06-2011
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Stability of radiolabelled cholecystokinin 2 (CCK2) receptor targeting peptides has been a major limitation in the use of such radiopharmaceuticals especially for targeted radionuclide therapy applications, e.g. for treatment of medullary thyroid carcinoma (MTC). The purpose of this study was to compare the in vitro stability of a series of peptides binding to the CCK2 receptor [selected as part of the COST Action on Targeted Radionuclide Therapy (BM0607)] and to identify major cleavage sites.
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Targeting properties of peptide-modified radiolabeled liposomal nanoparticles.
Nanomedicine
PUBLISHED: 02-25-2011
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Radiolabeled PEGylated liposomal nanoparticles (NPs) open new possibilities for a variety of applications including diagnosis, drug delivery, targeted therapy, and monitoring treatment effects. Here we describe the characterization of liposomal NPs (liposomes and micelles) derivatized with the somatostatin analogue tyrosine-3-octreotide as a proof of concept for tumor targeting. NPs were radiolabeled with indium-111, and targeting properties were evaluated in vitro on rat pancreatic tumor cells (AR42J), demonstrating specific binding and IC(50) values in the low nanomolar range. Biodistribution studies were performed in Lewis rats and compared to single-photon emission computed tomography images. Moderate tumor uptake was found in xenografted nude mice (<2.5% ID/g tissue) as compared to control. Micelles and liposomes revealed comparable pharmacokinetics and targeting properties. This study provides insight into tumor-targeting characteristics of peptide-derivatized liposomal NPs and can serve as a basis for further improvement of these constructs.
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[68Ga]NODAGA-RGD for imaging ?v?3 integrin expression.
Eur. J. Nucl. Med. Mol. Imaging
PUBLISHED: 02-11-2011
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A molecular target involved in the angiogenic process is the ?(v)?(3) integrin. It has been demonstrated in preclinical as well as in clinical studies that radiolabelled RGD peptides and positron emission tomography (PET) allow noninvasive monitoring of ?(v)?(3) expression. Here we introduce a (68)Ga-labelled NOTA-conjugated RGD peptide ([(68)Ga]NODAGA-RGD) and compare its imaging properties with [(68)Ga]DOTA-RGD using small animal PET.
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Influence of PEGylation and RGD loading on the targeting properties of radiolabeled liposomal nanoparticles.
Int J Nanomedicine
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Liposomes have been proposed to be a means of selectively targeting cancer sites for diagnostic and therapeutic applications. The focus of this work was the evaluation of radiolabeled PEGylated liposomes derivatized with varying amounts of a cyclic arginyl-glycyl-aspartic acid (RGD) peptide. RGD peptides are known to bind to ?(v)?(3) integrin receptors overexpressed during tumor-induced angiogenesis.
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[??Ga]NS?-RGD and [??Ga] Oxo-DO3A-RGD for imaging ?(v)?? integrin expression: synthesis, evaluation, and comparison.
Nucl. Med. Biol.
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??Ga-labeled RGD peptides in combination with PET allow non-invasive determination of ?(v)?? integrin expression which is highly increased during tumor-induced angiogenesis. The aim of this study was to synthesize and evaluate two RGD peptides containing alternative chelating systems, namely [??Ga]NS?-RGD-RGD and [??Ga]Oxo-DO3A-RGD and to compare their in vitro and in vivo properties with [??Ga]DOTA- and [??Ga]NODAGA-RGD.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.