Pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring.
An aerobic, Gram-stain-negative, rod-shaped bacterium (designated strain CC-G9A(T)), motile by a polar-flagellum, was isolated from a hot spring water sample in Taiwan. Strain CC-G9A(T) could grow at 20-42 °C, pH 6.0-10.0 and tolerate up to 7?% (w/v) NaCl. The 16S rRNA gene sequence analysis of strain CC-G9A(T) showed pairwise sequence similarity to Pseudomonas mendocina LMG 1223(T) (97.7?%), Pseudomonas alcaligenes ATCC 14909(T) (97.8?%), Pseudomonas alcaliphila DSM 17744(T) (97.8?%), Pseudomonas toyotomiensis JCM 15604(T) (97.6?%), Pseudomonas oleovorans subsp. lubricantis DSM 21016(T) (97.6?%) and Pseudomonas argentinensis BCRC 17807(T) (97.5?%), and lower sequence similarity to other species of the genus Pseudomonas. According to DNA-DNA association analysis, the relatedness of strain CC-G9A(T) to P. mendocina BCRC 10458(T), P. alcaliphila DSM 17744(T), P. alcaligenes BCRC 11893(T), P. oleovorans subsp. lubricantis DSM 21016(T), P. argentinensis BCRC 17807(T) and P. oleovorans subsp. oleovorans BCRC 11902 was 55.1±3.1, 13.7±1.5, 14.1±1.8, 58.5±1.1, 28.9±2.0 and 28.6±1.8?%, respectively. The evolutionary trees reconstructed based on 16S rRNA, gyrB and rpoB gene sequences revealed varying phylogenetic neighbourhoods of strain CC-G9A(T) with regard to the most closely related type strains. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 64.3±1.3 mol%. The major fatty acids were C10?:?0 3-OH, C12?:?0, C12?:?0 3-OH, C16?:?0 and summed features 3 and 8 consisting of C16?:?1?7c/C16?:?1?6c and C18?:?1?7c/C18?:?1?6c, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-G9A(T) is proposed to represent a novel species within the genus Pseudomonas for which the name Pseudomonas guguanensis sp. nov. is proposed. The type strain is CC-G9A(T) (?=?BCRC 80438(T)?=?JCM 18416(T)).