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Find video protocols related to scientific articles indexed in Pubmed.
Rhizobium alvei sp. nov., isolated from a freshwater river.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 11-12-2014
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A bacterial strain designated TNR-22T was isolated from freshwater river in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain TNR-22T were faculative anaerobic, Gram-stain-negative, rod-shaped, motile by a single polar flagellum and forming cream colored colonies. Growth occurred at 4-45 °C (optimum, 25-30 °C), with 0-1.0 % NaCl (optimum, 0.5 %) and at pH 7.0-8.0 (optimum, pH 7.0). Strain TNR-22T did not form nodules on a legume plant, Macroptilium atropurpureum. The nifH gene encoding denitrogenase reductase was not detected by PCR. The major fatty acids (?10 %) of strain TNR-22T were C18:1 ?7c and C16:0. The DNA G+C content was 60.3 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an uncharacterized aminoglycolipid and an uncharacterized phospholipid. Comparative analysis of the 16S rRNA gene sequences showed that strain TNR-22T constituted a distinct branch within the genus Rhizobium, showing the highest level of sequence similarity with respect to Rhizobium rosettiformans W3T (96.3 %). Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Rhizobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain TNR-22T represents a novel species in the genus Rhizobium, for which the name Rhizobium alvei sp. nov. is proposed. The type strain is TNR-22T (=BCRC 80408T =LMG 26895T =KCTC 23919T).
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Role of Shh and TGF in cyclosporine-enhanced expression of collagen and ?-SMA by gingival fibroblast.
J. Clin. Periodontol.
PUBLISHED: 11-12-2014
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Cyclosporine-A (CsA)-induced gingival overgrowth may arise from an alteration in stoma matrix homeostasis. Sonic hedgehog (Shh) plays a key role during embryogenic development and fibrotic progression, and may be involved in CsA-altered gingival matrix homeostasis.
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Cyclosporine-A Enhances Gingival ß-catenin Stability via Wnt Signaling.
J. Periodontol.
PUBLISHED: 10-30-2014
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Background: Cyclosporine-A (CsA) increases ß-catenin mRNA and protein expression. The present study demonstrates that Wingless-Int (Wnt)/ß-catenin signaling inhibits ß-catenin degradation in the gingiva. Methods: Forty 5-week-old male Sprague-Dawley rats were assigned to two study groups after healing from right maxillary molars extractions. The rats in the experimental group were fed 30 mg/kg CsA daily, whereas the control rats were fed mineral oil for 4 weeks. At the end of the study, all rats were sacrificed and the gingivae were obtained. The gingival morphology after CsA treatment was evaluated by histology, and the genes related to Wnt/ß-catenin signaling were initially screened by microarray. PCR, western blotting, and immunohistochemistry were used to examine the mRNA and protein expression of proliferating cell nuclear antigen (Pcna), cyclin D1, E-cadherin, ß-catenin, disheveled (Dvl)-1, glycogen synthase kinase 3? (GSK3?), Axin-1, and adenomatous polyposis coli (APC). Phosphoserine and ubiquitinylated ß-catenin were detected after immunoprecipitation. Results: In rats treated with CsA, overgrowth of gingivae were observed and altered expression of genes related to Wnt/?-catenin signaling was detected by the microarray. The gingival mRNA and protein expression profiles for genes associated with Wnt/?-catenin signaling further confirmed the effect of CsA: ?-catenin and Dvl-1 expression increased, but APC and Axin1 expression decreased. Western blotting and immunohistochemistry showed decreases in ß-catenin serine phosphorylation (33/37) and ubiquitinylation in the gingivae of CsA-treated rats. Conclusion: CsA-enhanced gingival ?-catenin stability may be involved in gene up-regulation or ?-catenin degradation via the Wnt/ß-catenin pathway.
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Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, An
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-30-2014
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A Gram-staining-negative, strictly aerobic, yellowish-orange-pigmented, flexirubin-positive, rod-shaped, non-flagellated, non-spore-forming and non-gliding marine bacterium, designated strain CC-PY-50T, was isolated from estuarine water off Pingtung, Taiwan. Strain produced zeaxanthin as a major carotenoid pigment, and showed highest pairwise 16S rRNA gene sequence similarity to Bizionia hallyeonensis T-y7T (93.9 %) followed by Corallibacter vietnamensis KMM 6217T (93.8 %), Geojedonia litorea YCS-16T (93.7 %) and other Flavobacteriaceae members (<93.7 %). Strain CC-PY-50T established a distinct phyletic lineage associated with Mangrovimonas yunxiaonensis LYYY01T (93.1 % sequence similarity) with poor bootstrap support during neighbor-joining and maximum-likelihood phylogenetic analyses (37 % for each). The polar lipid profile of strain CC-PY-50T characterized to accommodate large number of unknown lipids including major amounts of three unidentified aminolipids and two unidentified lipids, and moderate amounts of an unidentified phospholipid, an unidentified glycolipid and an unidentified lipid. In addition, phosphatidylethanolamine was also detected in significant amounts. The major (>5 % of total) fatty acids were iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH, C16:0 and C16:1 ?6c and/or C16:1 ?7c. The DNA G + C content was 37.1 mol% and menaquinone-6 (MK-6) was the sole respiratory quinone. Based on the phylogenetic evidence and several distinguishing phenotypic and chemotaxonomic features, strain CC-PY-50T is proposed to represent a novel genus and species of the family Flavobacteriaceae, for which the name Hanstruepera neustonica gen. nov., sp. nov. is proposed. The type strain of the type species Hanstruepera neustonica is CC-PY-50T (=JCM 19743T =BCRC 80747T). The emended descriptions of the species Sediminibacter furfurosus, Mangrovimonas yunxiaonensis, Antarcticimonas flava and Hoppeia youngheungensis are also proposed.
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Role of TGF-Beta1 on Cyclosporine-Induced Epithelial-to-Mesenchymal Transition in Gingival Epithelium.
J. Periodontol.
PUBLISHED: 10-03-2014
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Background: It has been proposed that cyclosporine (CsA) may induce epithelial-to-mesenchymal transition (EMT) in gingiva. The aims of the present study were to confirm the notion that EMT occurs in human gingival epithelial (hGE) cells after CsA treatment and to investigate the role of TGF-ß1 on this CsA-induced EMT. Methods: The effects of CsA, with and without TGF-?1 inhibitor, on the morphological changes of primary culture of hGE cells were in vitro examined. The changes of protein and mRNA expressions of two EMT markers (i.e. E-cadherin and ?-SMA) in the hGE cells after CsA treatment with and without TGF-?1 inhibitor were evaluated with immunocytochemistry and real time-PCR. Results: The epithelial cells became spindle-like, elongated, disassociated from neighboring cells, and lost their original cobblestone monolayer pattern when CsA was added. However, the epithelial cells stayed with their original cobblestone morphology when there was a treatment of TGF-?1 inhibitor on top of the CsA treatment. When CsA was given, the protein and mRNA expressions of E-cadherin and ?-SMA were significantly altered, and these alterations were significantly reversed when there was a pretreatment of TGF-?1 inhibitor. Conclusions: CsA could induce type 2 EMT in gingiva by changing the morphology of epithelial cells and altering the EMT markers/effectors. The CsA-induced gingival EMT is TGF-?1 dependent or at least partially dependent.
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Cribrihabitans neustonicus sp. nov., isolated from coastal surface seawater, and emended description of the genus Cribrihabitans Chen et al. 2014.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 09-01-2014
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A Gram-stain-negative, strictly aerobic, rod- or oval-shaped, motile, non-spore-forming bacterium, designated strain CC-AMHB-3(T), was isolated from coastal surface seawater off Hualien, Taiwan. The novel strain showed high pairwise 16S rRNA gene sequence similarities to Ruegeria mobilis NBRC 101030(T) (96.5?%), Ruegeria scottomollicae LMG 24367(T) (96.4?%), Phaeobacter aquaemixtae SSK6-1(T) (96.2?%), Phaeobacter daeponensis TF-218(T) (96.2?%), Cribrihabitans marinus CZ-AM5(T) (96.1?%) and other species of the family Rhodobacteraceae (?95.9?%). However, strain CC-AMHB-3(T) formed a distinct phyletic lineage associated with C. marinus CZ-AM5(T) during phylogenetic analyses. The polar lipid profile of strain CC-AMHB-3(T) included major amounts of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine; moderate amounts of phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid; and trace amounts of an unidentified lipid and an unidentified phospholipid, which was qualitatively almost in line with that of C. marinus CZ-AM5(T) but remarkably distinct as compared with the type species of the genera Ruegeria (Ruegeria atlantica JCM 21234(T)) and Phaeobacter (Phaeobacter gallaeciensis JCM 21319(T)). In line with the fatty acid profile of C. marinus CZ-AM5(T), the major (>5?% of total) fatty acids of strain CC-AMHB-3(T) were C18?:?1?7c and/or C18?:?1?6c (summed feature 8), 11-methyl C18?:?1?7c and C16?:?0. The DNA G+C content was 66.7 mol%. Ubiquinone-10 (Q-10) was the sole respiratory quinone. Thus, based on the results of the polyphasic study presented here, strain CC-AMHB-3(T) is considered to represent a novel species of the genus Cribrihabitans, for which the name Cribrihabitans neustonicus sp. nov. is proposed; the type strain is CC-AMHB-3(T) (?=?JCM 19537(T)?=?BCRC 80695(T)). In addition, an emended description of the genus Cribrihabitans is also proposed.
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Parametric laser pulse shortening.
Opt Lett
PUBLISHED: 08-15-2014
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We report simultaneous laser pulse shortening and wavelength conversion based on spectral-temporal correlation in high-gain optical parametric generation (OPG). By spectrally filtering the off-peak signal energy, we shortened a 560 ps pump pulse at 1064 nm to an 80 ps signal pulse at 1.5 ?m from a 45 mm long PPLN optical parametric generator with 60 ?J pump energy from a passively Q-switched Nd:YAG laser. Using the same technique, we further demonstrated a 3.6 time shortened laser pulse at 1072 nm from noncollinearly phase matched OPG in a 44 mm long lithium niobate crystal with 3 mJ amplified pump energy from the same Nd:YAG laser.
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Association of CCL5 and CCR5 Gene Polymorphisms With Periodontitis in Taiwanese.
J. Periodontol.
PUBLISHED: 08-14-2014
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It has been suggested that genetic factors may predispose individuals to periodontal diseases. The present case-control study aims to test whether the -403 single nucleotide polymorphism of chemokine ligand 5 (CCL5-403) and the 32-bp deletion of CCR5 (CCR5?32) polymorphisms are associated with susceptibility to chronic and aggressive periodontitis.
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Gelatinases and Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) are Associated With Cyclosporine-A Induced Attenuation of Periodontal Degradation in Rats.
J. Periodontol.
PUBLISHED: 08-14-2014
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Background: The present study aimed to exam the inhibitory effect of Cyclosporin-A (CsA) on periodontal destruction and to further explore the correlations of CsA-induced attenuation of periodontal bone loss with the expressions of gelatinases (i.e. matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9)) and their inducer, extracellular matrix metalloproteinase inducer (EMMPRIN). Methods: Forty SD rats were randomly divided into four groups: control, CsA, ligature (Lig), and ligature-plus-CsA (Lig+CsA) groups. CsA group received 10 mg/Kg/day of CsA for 8 days. Lig group received silk ligature on selected molars. Lig+CsA group received silk ligature and CsA treatment. The inhibitory effects of CsA on the ligature-induced periodontal destruction was examined with micro-CT and histometric analysis to analyze the amount of attachment loss, crestal bone loss, connective tissue attachment, and the surface area with inflammatory cell infiltration. The effects of CsA on ligature-induced expressions of gelatinases and EMMPRIN in gingival tissues were examined with Western blotting and zymography, respectively. Results: By micro-CT and histology, Lig+CsA group had significantly more periodontal destruction than control and CsA groups, but less periodontal destruction than Lig group. Consistent results were found for the expressions of gelatinases and EMMPRIN among the groups demonstrating that Lig+CsA group had significantly less gingival protein expression of gelatinases and EMMPRIN than Lig group. Conclusion: CsA inhibited the expressions of gelatinase MMPs and EMMPRIN, and partially prevented the periodontal destruction in ligature-induced experimental periodontitis. The CsA-induced attenuation of periodontal bone loss was strongly correlated positively with the expressions of MMP-2, MMP-9, and EMMPRIN in gingiva.
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Nutrient intake and use of dietary supplements among US adults with disabilities.
Disabil Health J
PUBLISHED: 06-27-2014
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Physical, mental, social, and financial hurdles in adults with disabilities may limit their access to adequate nutrition.
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Description of Algoriphagus taiwanensis sp. nov., a xylanolytic bacterium isolated from surface seawater, and emended descriptions of Algoriphagus mannitolivorans, Algoriphagus olei, Algoriphagus aquatilis and Algoriphagus ratkowskyi.
Antonie Van Leeuwenhoek
PUBLISHED: 06-17-2014
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A novel, Gram-stain negative, strictly aerobic, oval or rod-shaped, red-pigmented, non-spore-forming xylanolytic bacterial strain, designated CC-PR-82(T), was isolated from surface seawater of Pingtung coast, Taiwan, and characterized by polyphasic taxonomy. Strain CC-PR-82(T) shared highest pairwise 16S rRNA gene sequence similarity to Algoriphagus mannitolivorans IMSNU 14012(T) (97.0 %) followed by 'A. boseongensis' BS-R1(T) (96.5 %) and A. olei CC-Hsuan-617(T) (95.6 %), whereas other (n = 26) Algoriphapus species shared 95.6-92.6 % sequence similarities. The novel strain further established a distinct phyletic lineage tightly associated with Algoriphagus species. The DNA-DNA hybridization value obtained between CC-PR-82(T) and A. mannitolivorans DSM 15301(T) was 29.0 % (33.4 % reciprocal using A. mannitolivorans DSM 15301(T) probe). The major (>5 % of total) fatty acids were identified as iso-C15:0, C16:1 ?6c and/or C16:1 ?7c, iso-C16:0, iso-C17:1 ?9c and/or C16:0 10-methyl, anteiso-C15:0 and C17:1 ?6c. Polar lipids included major amounts of an unidentified aminolipid and an unidentified lipid; moderate amounts of phosphatidylethanolamine, phosphatidylserine and an unidentified aminolipid; trace amounts of an unidentified phospholipid, an unidentified lipid and an unidentified glycolipid. The DNA G+C content was determined to be 42.3 mol%. Menaquinone-7 was the sole respiratory quinone. Based on the polyphasic characteristics, that are in line with those of Algoriphagus species, in addition to distinguishing phylogenetic and phenotypic features, strain CC-PR-82(T) appears to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus taiwanensis sp. nov. (type strain CC-PR-82(T) = JCM 19755(T) = BCRC 80746(T)) is proposed. In addition, emended descriptions of the species A. mannitolivorans, A. aquatilis, A. olei and A. ratkowskyi are also proposed.
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The priming effect of military service on creativity performance.
Psychol Rep
PUBLISHED: 06-06-2014
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This study investigated the service priming effect on creativity performance. In three experiments, the service priming was manipulated in three ways (Army priming, Air Force priming, and a Neutral condition). Participants' performances on the Chinese Remote Associates Test (CRAT), insight problems, and critical thinking problems were accordingly measured in each experiment. Results showed that the Air Force priming improved creativity and the Army priming enhanced critical thinking. The results suggest that the constructions and processes of these two manipulations are different. In addition, results also suggested that the branch of military service moderates the relationship between the service priming and the performance of creativity.
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Rhizobium straminoryzae sp. nov., isolated from the surface of rice straw.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 06-04-2014
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An aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain CC-LY845(T), was isolated from the surface of rice straw in Taiwan. Cells were non-motile, and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus Rhizobium, with closest similarity to Rhizobium pseudoryzae KCTC 23294(T) (97.6?%), R. rhizoryzae KCTC 23652(T) (97.0?%) and R. oryzae LMG 24253(T) (96.7?%); other species showed lower levels of similarity (<96.6?%). The DNA-DNA relatedness of strain CC-LY845(T) and R. pseudoryzae KCTC 23294(T) was 34.8±3.1?% (reciprocal value 39.2±2.2?%). Phylogenetic analysis based on the housekeeping atpD and recA genes showed that the novel strain could be distinguished from R. pseudoryzae KCTC 23294(T) (92.7 and 91.5?%, respectively) and other species of the genus Rhizobium. The temperature range for growth was 25-42 °C, the pH range was 5.0-9.0 and NaCl concentrations up to 4.0?% (w/v) were tolerated. Strain CC-LY845(T) did not form nodules on four different legumes, and the nodD and nifH genes were not detected by PCR. The major fatty acids were C16?:?0 and summed feature 8 (C18?:?1?7c/C18?:?1?6c). The polyamine pattern of strain CC-LY845(T) showed spermidine and putrescine as major polyamines. The predominant quinone system was ubiquinone 10 (Q-10). The DNA G+C content was 68.3±2.4 mol%. Base on its phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY845(T) is proposed to represent a novel species within the genus Rhizobium, for which the name Rhizobium straminoryzae sp. nov. is proposed. The type strain is strain CC-LY845(T) (?=?BCRC 80698(T)?=?JCM 19536(T)).
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Dexamethasone reduces brain cell apoptosis and inhibits inflammatory response in rats with intracerebral hemorrhage.
J. Neurosci. Res.
PUBLISHED: 05-30-2014
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Spontaneous intracerebral hemorrhage (ICH) is associated with high rates of mortality and morbidity. Thus, the identification of novel therapeutic agents for preventing strokes and attenuating poststroke brain damage is crucial. Dexamethasone (DEX) is used clinically to reduce edema formation in patients with spinal cord injury and brain tumors. In this study, we sought to elucidate the effects of DEX treatment on apoptosis and inflammation following ICH in rats. A high dose of DEX (15 mg/kg) was administered immediately following ICH induction and again 3 days later. The inflammatory and apoptotic responses in the rat brains were evaluated by using hematoxylin-eosin, terminal deoxynucleotidyl transferase dUTP nick end labeling, Nissl, and neurofilament-H staining. Levels of phosphorylated neurofilaments and apoptosis-related proteins such as B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax), caspase-3, and P53 were analyzed by Western blotting. This study shows that rats without ICH that received DEX treatment had a fourfold higher expression of Bcl-2 than sham-operated rats. ICH causes an increase in Bax, cleaved caspase-3, and P53 proteins from 4 hr to 7 days following ICH induction. In comparison with the ICH rats, the ICH/DEX rats showed significantly decreased apoptotic cell death and increased neuron survival and maintained neurofilament integrity in the perihematomal region. DEX increased the Bcl-2/Bax ratio and lowered the expression of cleaved caspase-3 at 12 hr and 5 days. The ICH rats were accompanied by activation of the inflammatory response, and DEX treatment modulated the expression of a variety of cell types and then decreased ICH-induced apoptosis. © 2014 Wiley Periodicals, Inc.
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Gramella oceani sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from marine sediment.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 05-16-2014
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A Gram-staining-negative, yellow-pigmented, strictly aerobic, zeaxanthin-producing, rod-shaped, non-endospore-forming, appendaged bacterial strain that exhibits gliding motility, designated CC-AMSZ-T(T), was isolated from marine sediment off coastal Kending, Taiwan. Strain CC-AMSZ-T(T) shared 94.9% and 96.7-94.1% 16S rRNA gene sequence similarities with Gramella echinicola KMM 6050(T) and other species of the genus Gramella, respectively, and formed a distinct phyletic lineage in phylogenetic trees. The major (?5% of the total) fatty acids were C(16?:?0), iso-C(15?:?0), anteiso-C(15?:?0), C(16?:?1)?6c and/or C(16?:?1)?7c and iso-C(17?:?1)?9c and/or C(16?:?0) 10-methyl. Phosphatidylethanolamine, six unidentified lipids and three unidentified aminolipids were the polar lipid components. The DNA G+C content was 38.6 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic distinctiveness and distinguishing phenotypic characteristics, strain CC-AMSZ-T(T) represents a novel species of the genus Gramella, for which the name Gramella oceani sp. nov. is proposed. The type strain is CC-AMSZ-T(T) (?=?JCM 18809(T)?=?BCRC 80547(T)).
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Paludibacterium paludis sp. nov., isolated from a marsh.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 05-06-2014
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A bacterial strain, designated KBP-21(T), was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain KBP-21(T) were Gram-stain-negative, facultatively anaerobic, poly-?-hydroxybutyrate-accumulating, motile rods that formed yellow colonies. Growth occurred at 15-40 °C (optimum, 30 °C), at pH 5.0-8.0 (optimum, pH 8.0) and with 0-2% NaCl (optimum, 0%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-21(T) belonged to the genus Paludibacterium within the family Neisseriaceae of the class Betaproteobacteria and the closest related neighbour was Paludibacterium yongneupense 5YN8-15(T) with a 16S rRNA gene sequence similarity value of 96.4%. Strain KBP-21(T) contained summed feature 3 (comprising C16 : 1?7c and/or C16 : 1?6c), C16 : 0 and C18 : 1?7c as the predominant fatty acids. The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 62.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one uncharacterized aminophospholipid and several uncharacterized phospholipids. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain KBP-21(T) represents a novel species in the genus Paludibacterium, for which the name Paludibacterium paludis sp. nov. is proposed. The type strain is KBP-21(T) (?= BCRC 80514(T)? = LMG 27230(T) ?= KCTC 32182(T)).
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Interface engineering for high-performance top-gated MoS2 field-effect transistors.
Adv. Mater. Weinheim
PUBLISHED: 05-05-2014
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Experimental evidence of the optimized interface engineering effects in MoS2 transistors is demonstrated. The MoS2/Y2O3/HfO2 stack offers excellent interface control. Results show that HfO2 layer can be scaled down to 9 nm, yet achieving a near-ideal sub-threshold slope (65 mv/dec) and the highest saturation current (526 ?A/?m) of any MoS2 transistor reported to date.
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Revealing controllable nanowire transformation through cationic exchange for RRAM application.
Nano Lett.
PUBLISHED: 04-21-2014
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One dimensional metal oxide nanostructures have attracted much attention owing to their fascinating functional properties. Among them, piezoelectricity and photocatalysts along with their related materials have stirred significant interests and widespread studies in recent years. In this work, we successfully transformed piezoelectric ZnO into photocatalytic TiO2 and formed TiO2/ZnO axial heterostructure nanowires with flat interfaces by solid to solid cationic exchange reactions in high vacuum (approximately 10(-8) Torr) transmission electron microscope (TEM). Kinetic behavior of the single crystalline TiO2 was systematically analyzed. The nanoscale growth rate of TiO2 has been measured using in situ TEM videos. On the basis of the rate, we can control the dimensions of the axial-nanoheterostructure. In addition, the unique Pt/ ZnO / TiO2/ ZnO /Pt heterostructures with complementary resistive switching (CRS) characteristics were designed to solve the important issue of sneak-peak current. The resistive switching behavior was attributed to the migration of oxygen and TiO2 layer served as reservoir, which was confirmed by energy dispersive spectrometry (EDS) analysis. This study not only supplied a distinct method to explore the transformation mechanisms but also exhibited the potential application of ZnO/TiO2 heterostructure in nanoscale crossbar array resistive random-access memory (RRAM).
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Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 04-17-2014
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Two Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strains that are motile by a monopolar flagellum, designated CC-AMH-11(T) and CC-AMHZ-5, were isolated from droppings of a seashore bird off the coast of Hualien, Taiwan. The strains showed 99.7% mutual pairwise 16S rRNA gene sequence similarity, while exhibiting <96.2% sequence similarity to strains of other species of the genus Pseudomonas (95.7-95.9% similarity with type species, Pseudomonas aeruginosa LMG 1242T), and formed a distinct co-phyletic lineage in the phylogenetic trees. The common major fatty acids (>5% of the total) were C18 : 1?7c and/or C18 : 1?6c (summed feature 8), C16 : 1?6c and/or C16 : 1?7c (summed feature 3), C16 : 0 and C12 : 0. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine, an unidentified lipid and an unidentified phospholipid were detected as common polar lipids. The DNA G+C contents of strains CC-AMH-11(T) and CC-AMHZ-5 were 61.1 and 61.6 mol%, respectively. The common major respiratory quinone was ubiquinone 9 (Q-9), and the predominant polyamine was putrescine. The DNA-DNA hybridization obtained between the two strains was 79.0% (reciprocal value 89.4% using CC-AMHZ-5 DNA as the probe). The very high 16S rRNA gene sequence similarity and DNA-DNA relatedness and the poorly distinguishable phenotypic features witnessed between CC-AMH-11(T) and CC-AMHZ-5 suggested unambiguously that they are two distinct strains of a single genomic species. However, the strains also showed several genotypic and phenotypic characteristics that distinguished them from other closely related species of Pseudomonas. Thus, the strains are proposed to represent a novel species of Pseudomonas, for which the name Pseudomonas hussainii sp. nov. is proposed. The type strain is CC-AMH-11(T) (?= JCM 19513(T)?= BCRC 80696(T)); a second strain of the same species is CC-AMHZ-5 (?= JCM 19512 = BCRC 80697). In addition, emended descriptions of the species Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis are also proposed.
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Description of Niveispirillum fermenti gen. nov., sp. nov., isolated from a fermentor in Taiwan, transfer of Azospirillum irakense (1989) as Niveispirillum irakense comb. nov., and reclassification of Azospirillum amazonense (1983) as Nitrospirillum amazonense gen. nov.
Antonie Van Leeuwenhoek
PUBLISHED: 04-15-2014
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A taxonomic study was carried out on a novel aerobic bacterial strain (designated CC-LY736(T)) isolated from a fermentor in Taiwan. Cells of strain CC-LY736(T) were Gram-stain negative, spiral-shaped and motile by means of a monopolar flagellum. Strain CC-LY736(T) shared the greatest degree of 16S rRNA gene sequence similarity to Azospirillum irakense DSM 11586(T) (97.2 %), Rhodocista centenaria JCM 21060(T) (96.3 %) and Rhodocista pekingensis JCM 11669(T) (96.1 %). The major fatty acids were C16:0, C16:1 ?5c, C19:0 cyclo ?8c, C18:1 ?7c/C18:1 ?6c, C16:0 3-OH and C18:1 2-OH. The predominant polar lipids included phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine and two unidentified glycolipids. The common major respiratory quinone was ubiquinone Q-10 and predominant polyamines were sym-homospermidine and putrescine. The DNA G+C content of strain CC-LY736(T) was 67.6 ± 0.1 mol %. During phylogenetic analysis, strain CC-LY736(T) formed a unique phyletic lineage associated with Rhodocista species. However, the combination of genetic, chemotaxonomic and physiological data clearly indicated that strain CC-LY736(T) was a novel representative of the family Rhodospirillaceae. Based on the polyphasic comparison, the name Niveispirillum fermenti gen. nov., sp. nov. is proposed; the type strain of the type species is CC-LY736(T) (= BCRC 80504(T) = LMG 27263(T)). In addition, the reclassifications of Azospirillum irakense as Niveispirillum irakense comb. nov. (type strain KBC1(T) = ATCC 51182(T) = BCRC 15764(T) = CIP 103311(T)), and Azospirillum amazonense as Nitrospirillum amazonense gen. nov., sp. nov. (type strain Am14(T) = ATCC 35119(T) = BCRC 14279(T) = DSM 3787(T)) are proposed based on the polyphasic taxonomic data obtained in this study.
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Real time observation of the formation of hollow nanostructures through solid state reactions.
Anal. Chem.
PUBLISHED: 04-08-2014
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We demonstrate the formation of hollow nickel germanide nanostructures of Ni-Ge core-shell nanoparticles by solid state reactions. The structural evolutions of nickel germanide hollow nanostructures have been investigated in real-time ultrahigh vacuum transmission electron microscopy (UHV-TEM). Annealed above 450 °C, the nonequilibrium interdiffusion of core and shell species occurred at the interface; thus, Ni germanide hollow nanostructures were formed by solid state reactions involving the Kirkendall effect. In addition, the different hollow nanostructures formed from different core diameters of Ni-Ge core-shell nanoparticles have been studied. Also, we propose the mechanism with effects of the size and annealing duration on the solid state reactions based on the Kirkendall effect.
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High glucose activates ChREBP-mediated HIF-1? and VEGF expression in human RPE cells under normoxia.
Adv. Exp. Med. Biol.
PUBLISHED: 03-26-2014
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Because retina-damaging angiogenesis is controlled by vascular endothelial growth factor (VEGF) and people with higher glucose intakes are more susceptible to retinal complications that may be due to increased VEGF, it is crucial to elucidate relations between glucose exposure and VEGF expression. We aimed to determine if a carbohydrate response element binding protein (ChREBP) plays a role in the transcriptional up-regulation of hypoxia-inducible factor-1? (HIF-1?) and the downstream VEGF expression in retinal pigment epithelial (RPE) cells exposed to high glucose under normoxic conditions.
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Effect of oral appliance on endothelial function in sleep apnea.
Clin Oral Investig
PUBLISHED: 03-17-2014
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This study evaluated the effects of mandibular advancement device (MAD) on serum levels of nitric oxide derivatives and endothelial function by endothelium-dependent flow-mediated dilation (FMD) in obstructive sleep apnea syndrome (OSAS).
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Rivicola pingtungensis gen. nov., sp. nov., a new member of the family Neisseriaceae isolated from a freshwater river.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 03-17-2014
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A bacterial strain, designated Npb-03(T), was isolated from a freshwater river in Taiwan and was characterized using a polyphasic taxonomic approach. The cells were Gram-reaction-negative, straight rod-shaped, non-motile, non-spore-forming and facultatively anaerobic. Growth occurred at 10-37 °C (optimum, 30-35 °C), at pH 6.0-8.0 (optimum, pH 6.0-7.0) and with 0-1.0% NaCl (optimum, 0%). The predominant fatty acids were summed feature 3 (comprising C(16?:?1)?7c and/or C(16?:?1)?6c) and C(16?:?0). The major isoprenoid quinone was Q-8 and the DNA G+C content was 64.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and three uncharacterized phospholipids. The major polyamines were putrescine, 2-hydroxyputrescine, cadaverine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-03(T) forms a distinct lineage with respect to closely related genera within the family Neisseriaceae of the class Betaproteobacteria, most closely related to the genera Aquaspirillum, Laribacter, Leeia and Microvirgula, and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93%. On the basis of the genotypic and phenotypic data, strain Npb-03(T) represents a novel genus and species of the family Neisseriaceae, for which the name Rivicola pingtungensis gen. nov., sp. nov. is proposed. The type strain is Npb-03(T) (?=?BCRC 80376(T)?=?LMG 26668(T)?=?KCTC 23712(T)).
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Effect of uvulopalatopharyngoplasty on leptin and endothelial function in sleep apnea.
Ann. Otol. Rhinol. Laryngol.
PUBLISHED: 02-28-2014
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This study evaluated the effects of uvulopalatopharyngoplasty (UPPP) on serum leptin levels and endothelial function in patients with obstructive sleep apnea syndrome (OSAS).
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N-isopropylacrylamide gel dosimeter to evaluate clinical photon beam characteristics.
Appl Radiat Isot
PUBLISHED: 02-14-2014
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The introduction of beam intensity control concept in current radiotherapy techniques has increased treatment planning complexity. Thus, small-field dose measurement has become increasingly vital. Polymer gel dosimetry method is widely studied. It is the only dose measurement tool that provides 3D dose distribution. This study aims to use an N-isopropylacrylamide (NIPAM) gel dosimeter to conduct beam performance measurements of percentage depth dose (PDD), beam flatness, and symmetry for photon beams with field sizes of 3×3 and 4×4 cm(2). Computed tomography scans were used to readout the gel dosimeters. In the PDD measurement, the NIPAM gel dosimeter and Gafchromic™ EBT3 radiochromic film displayed high consistency in the region deeper than the build-up region. The gel dosimeter dose profile had 3% lower flatness and symmetry measurement at 5 cm depth for different fields compared with that of the Gafchromic™ EBT3 film. During gamma evaluation under 3%/3 mm dose difference/distance-to-agreement standard, the pass rates of the polymer gel dosimeter to the TPS and EBT3 film were both higher than 96%. Given that the gel is tissue equivalent, it did not exhibit the energy dependence problems of radiochromic films. Therefore, the practical use of NIPAM polymer gel dosimeters is enhanced in clinical dose verification.
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The relationship of major American dietary patterns to age-related macular degeneration.
Am. J. Ophthalmol.
PUBLISHED: 02-12-2014
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We hypothesized that major American dietary patterns are associated with risk for age-related macular degeneration (AMD).
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Gramella planctonica sp. nov., a zeaxanthin-producing bacterium isolated from surface seawater, and emended descriptions of Gramella aestuarii and Gramella echinicola.
Antonie Van Leeuwenhoek
PUBLISHED: 01-30-2014
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A Gram-stain negative, strictly aerobic, zeaxanthin-producing, rod-shaped, non-spore-forming bacterial strain which is motile by gliding, designated CC-AMWZ-3(T), was isolated from surface seawater off coastal Kending, Taiwan. Strain CC-AMWZ-3(T) was found to share 93.3 % and 96.0-92.4 % pairwise 16S rRNA gene sequence similarity to Gramella echinicola KMM 6050(T) and other Gramella species, respectively, and formed distinct phyletic lineage during phylogenetic analysis. The major fatty acids were identified as C16:0, iso-C15:0, anteiso-C15:0, C16:1 ?6c and/or C16:1 ?7c and iso-C17:1 ?9c and/or C16:0 10-methyl. Polar lipids were found to include phosphatidylethanolamine, six unidentified lipids and three unidentified aminolipids. The DNA G+C content was determined to be 40.6 mol%. Menaquinone-6 was the sole respiratory quinone identified and triamine-sym-homospermidine was the predominant polyamine. Based on the polyphasic characteristics that are in line with those of Gramella species, in addition to distinguishing phylogenetic and phenotypic features, strain CC-AMWZ-3(T) appears to represent a novel species of the genus Gramella, for which the name Gramella planctonica sp. nov. (type strain CC-AMWZ-3(T) = JCM 18807(T) = BCRC 80553(T)) is proposed. In addition, emended descriptions of the species Gramella aestuarii and Gramella echinicola are also proposed.
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Effects of enamel matrix derivative on the proliferation and osteogenic differentiation of human gingival mesenchymal stem cells.
Stem Cell Res Ther
PUBLISHED: 01-28-2014
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Gingiva-derived mesenchymal stem cells (GMSCs) have recently been harvested and applied for rebuilding lost periodontal tissue. Enamel matrix derivative (EMD) has been used for periodontal regeneration and the formation of new cementum with inserting collagen fibers; however, alveolar bone formation is minimal. Recently, EMD has been shown to enhance the proliferation and mineralization of human bone marrow mesenchymal stem cells. Because the gingival flap is the major component to cover the surgical wound, the effects of EMD on the proliferation and mineralization of GMSCs were evaluated in the present study.
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Prognosis of patients with acute respiratory distress syndrome on extracorporeal membrane oxygenation: the impact of urine output on mortality.
Ann. Thorac. Surg.
PUBLISHED: 01-27-2014
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Extracorporeal membrane oxygenation (ECMO) has been utilized for patients in critical condition, including life-threatening respiratory failure and postcardiotomy cardiogenic shock. This study analyzed the outcomes of patients with acute respiratory distress syndrome (ARDS) treated by ECMO and identified the relationship between prognosis and urine output (UO) obtained on the first day of ECMO support.
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A risk score for the prediction of advanced age-related macular degeneration: development and validation in 2 prospective cohorts.
Ophthalmology
PUBLISHED: 01-13-2014
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To develop a clinical eye-specific prediction model for advanced age-related macular degeneration (AMD).
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Impacts of industrial waste resources on maize (Zea mays L.) growth, yield, nutrients uptake and soil properties.
Waste Manag
PUBLISHED: 01-09-2014
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Discharging untreated highly acidic (pH<4.0), organic and nutrients rich monosodium glutamate wastewater (MW), and highly alkaline (pH>10.0) paper-mill wastewater (PW) causes environmental pollution. When acidity of MW neutralized (pH 6.5±0.1) with PW and lime (treatments represented as MW+PW and MW+Lime), then MW may be utilized as a potential source of nutrients and organic carbon for sustainable food production. Objectives of this study were to compare the effects of PW and lime neutralized MW and chemical fertilizers on maize (Zea mays L. cv. Snow Jean) plant growth, yield, nutrients uptake, soil organic matter and humic substances. The field experiment was carried out on maize using MW at 6000 L ha(-1). Impacts of the MW application on maize crop and soil properties were evaluated at different stages. At harvest, plant height, and plant N and K uptake were higher in MW treatment. Leaf area index at 60 days after sowing, plant dry matter accumulation at harvest, and kernels ear(-1) and 100-kernel weight were higher in MW+Lime treatment. Kernel N, P, K, Mn, Fe and Zn, and plant Zn uptake were highest in MW+Lime. Plant Fe uptake, and soil organic matter and humic substances were highest in MW+PW. The MW+PW and MW+Lime treatments exhibited comparable results with chemically fertilized treatment. The MW acidity neutralized with lime showed positive impacts on growth, yield and nutrients uptake; nevertheless, when MW pH neutralized with PW has an additional benefit on increase in soil organic matter and humic substances.
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Novosphingobium arabidopsis sp. nov., a novel DDT-resistant bacterium isolated from the Arabidopsis thaliana rhizosphere.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-24-2013
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An aerobic, Gram-stained-negative, rod-shaped, DDT-resistant bacterium (defined strain CC-ALB-2T) was isolated from the Arabidopsis thaliana rhizosphere. Strain CC-ALB-2T is able to grow at 25-37°C, pH 5.0-8.0, NaCl 1.0% (w/v) and tolerate DDT up to 200 mg l-1. The 16S rRNA gene sequence analysis of strain CC-ALB-2T showed highest sequence similarity to Novosphingobium stygium KCTC 2891T (97.1%) and Novosphingobium soli DSM 22821T (96.8%), and showed lower levels of similarity (<97.0%) to other Novosphingobium species. The major fatty acid profile consists of C14:0 2OH (13.1%), C16:0 (10.0%), C15:0 iso 3OH (5.8%), C16:1 ?7c / C16:1 ?6c (24.7%) and C18:1 ?7c / C18:1 ?6c (42.4%). The polar lipid profile constitutes sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylglycerol and phosphatidyl choline. The polyamine pattern showed predominance of spermidine as major polyamine. The predominant quinone system is ubiquinone (Q-10). The DNA G+C content is 68.9±0.1 mol%. Base on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-ALB-2T is proposed to represent a novel species within the genus Novosphingobium for which the name Novosphingobium arabidopsis sp. nov. (=BCRC 80571T =JCM 18896T) is proposed.
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Robertkochia marina gen. nov., sp. nov., of the family Flavobacteriaceae isolated from surface seawater, and emended descriptions of the genera Joostella and Galbibacter.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-14-2013
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A Gram-staining-negative, orange-pigmented, strictly aerobic, carotenoid-producing, rod-shaped, non-flagellated, non-spore-forming bacterium motile by gliding designated strain CC-AMO-30DT was isolated from surface seawater collected near Taichung harbor, Taiwan. Strain CC-AMO-30DT shared pairwise 16S rRNA gene sequence similarities of 94.8, 93.7 and 92.5 % to the type species of the genera Joostella, Pustulibacterium and Galbibacter, respectively, and formed a distinct monophyletic lineage in phylogenetic trees. The major fatty acids (? 5 % of total fatty acids) were iso-C 15:1 G, iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH, iso-C16:0 and anteiso-C15:0. Polar lipid profile consisted of phosphatidylethanolamine, four unidentified lipids, two unidentified aminolipids and an unidentified phospholipid. The major polyamine was triamine sym-homospermidine. The DNA G + C content was 47.1 mol% and the predominant respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic distinctiveness, and distinguishing phenotypic and chemotaxonomic characteristics, strain CC-AMO-30DT represents a novel genus and species of the family Flavobacteriaceae, for which the name Robertkochia marina gen. nov., sp. nov. is proposed; the type strain of the type species is CC-AMO-30DT (= JCM 18552T = BCRC 80469T). Emended descriptions of the genera Joostella and Galbibacter are also proposed.
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Chitinophaga taiwanensis sp. nov., a novel bacterium isolated from the Arabidopsis thaliana rhizosphere.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-04-2013
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An aerobic, Gram-negative, rod-shaped bacterium (strain CC-ALB-1T) was isolated from the Arabidopsis thaliana rhizosphere. Strain CC-ALB-1T is able to grow at 20-30 °C, pH 5.0-8.0 and NaCl 1.0% (w/v). The 16S rRNA gene sequence analysis of strain CC-ALB-1T showed highest sequence similarity to Chitinophaga ginsengisegetis KCTC 12654T (96.9%) and Chitinophaga niastensis JCM 15441T (96.7%), and other species showed lower levels of similarity (<97.0%) to all other Chitinophaga species. The fatty acid profile consists of C15:0 iso, C17:0 iso 3OH, C15:1 ?5c, C16:1 ?5c and C16:1 ?7c/C16:1 ?6c. The polar lipid profile constitutes phosphatidylethanolamine (PE), unidentified aminolipids (AL1-2) and unidentified lipids (L1-5). The predominant quinone system is menaquinone (MK-7). The DNA G+C content is 53.4±0.4 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-ALB-1T is proposed to represent a novel species within the genus Chitinophaga for which the name Chitinophaga taiwanensis sp. nov. (=BCRC 80570T =JCM 18895T) is proposed.
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Aquibacter zeaxanthinifaciens gen. nov., sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from surface seawater, and emended descriptions of the genera Aestuariibaculum and Gaetbulibacter.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 09-18-2013
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A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-304T was isolated from coastal surface seawater near Taichung harbor, Taiwan. Strain CC-AMZ-304T predominantly synthesized zeaxanthin and thus formed yellow-colored colonies on marine agar. The novel strain showed unstable phylogenetic position, although sharing high pairwise 16S rRNA gene sequence similarities of 95.9-94.9, 95.7 and 95.1-93.9 % with Gaetbulibacter species (n=4), Aestuariibaculum suncheonense SC17T and Bizionia species (n=7), respectively. The polar lipid profile of strain CC-AMZ-304T consisted of phosphatidylethanolamine, five unidentified lipids, one unidentified phospholipid, two unidentified aminolipids and one unidentified glycolipid. The major (> 5 % of the total) fatty acids were C15:0 iso, C15:1 iso G, C17:0 iso 3-OH, C15:0 iso 3-OH and C 15:1 ?5c. The DNA G + C content was 36.0 mol%. Menaquinone-6 (MK-6) was the sole respiratory quinone and the major polyamine was triamine sym-homospermidine. Phylogenetic distinctiveness, unique polar lipid composition, presence of significant amounts of branched hydroxyl fatty acids (C17:0 iso 3-OH and C15:0 iso 3-OH ) and of low amount of C15:0 anteiso, and several additional distinguishing biochemical features clearly discriminated strain CC-AMZ-304T from the type species of the genera Aestuariibaculum and Gaetbulibacter. Thus, based on the present polyphasic evidences, strain CC-AMZ-304T is proposed to represent a novel genus and species within the family Flavobacteriaceae, for which the name Aquibacter zeaxanthinifaciens gen. nov., sp. nov. proposed; the type strain is of the type species is CC-AMZ-304T (= JCM 18557T = BCRC 80463T). Emended descriptions of the genera Aestuariibaculum and Gaetbulibacter are also proposed.
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Lacibacterium aquatile gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a freshwater lake.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 09-03-2013
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A bacterial strain designated LTC-2(T) was isolated from a freshwater lake in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain LTC-2(T) were Gram-reaction-negative, facultatively anaerobic, poly-?-hydroxybutyrate-accumulating, motile by means of a monopolar flagellum, non-spore-forming, slightly curved rods surrounded by a thick capsule and formed creamy white colonies. Growth occurred at 10-37 °C (optimum, 20-30 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and with 0-1.0?% NaCl (optimum, 0?%). The predominant fatty acids were C18?:?1?7c, summed feature 3 (comprising C16?:?1?7c and/or C16?:?1?6c) and C16?:?0. The major isoprenoid quinone was Q-10 and the DNA G+C content was 58.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, two uncharacterized phospholipids and two uncharacterized aminophospholipids. The major polyamines were putrescine, homospermidine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain LTC-2(T) forms a distinct lineage with respect to closely related genera in the family Rhodospirillaceae, most closely related to the genera Elstera and Dongia, and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera were less than 94?%. On the basis of the genotypic and phenotypic data, strain LTC-2(T) represents a novel genus and species of the family Rhodospirillaceae, for which the name Lacibacterium aquatile gen. nov., sp. nov. is proposed. The type strain is LTC-2(T) (?=?BCRC 80445(T)?=?LMG 26999(T)?=?KCTC 32017(T)).
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Kordia aquimaris sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from surface seawater, and emended description of the genus Kordia.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-30-2013
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A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-301(T) was isolated from coastal surface seawater near Taichung harbour, Taiwan. Strain CC-AMZ-301(T) predominantly synthesized zeaxanthin and thus formed yellow colonies on marine agar. The novel strain showed high pairwise 16S rRNA gene sequence similarity to Kordia periserrulae IMCC1412(T) (95.0?%), K. algicida KCTC 8814P(T) (94.4?%) and K. antarctica IMCC3317(T) (94.2?%), and formed a phylogenetic lineage tightly associated with species of the genus Kordia. The polar lipid profile of strain CC-AMZ-301(T) consisted of three unidentified lipids, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids (>5?% of total) were iso-C17?:?0 3-OH, iso-C15?:?0, summed feature 3 (C16?:?1?7c and/or C16?:?1?6c) and summed feature 9 (iso-C17?:?1?9c and/or 10-methyl C16?:?0). The DNA G+C content was 36.4 mol%. Menaquinone 6 (MK-6) was the predominant respiratory quinone. The major polyamine was the triamine sym-homospermidine. Based on evidence from the present polyphasic study, strain CC-AMZ-301(T) is proposed to represent a novel species of the genus Kordia, for which the name Kordia aquimaris sp. nov. is proposed; the type strain is CC-AMZ-301(T) (?=?JCM 18556(T)?=?BCRC 80464(T)). An emended description of the genus Kordia is also proposed.
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Luteibaculum oceani gen. nov., sp. nov., a carotenoid-producing, lipolytic bacterium isolated from surface seawater, and emended description of the genus Owenweeksia Lau et al. 2005.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-29-2013
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A yellow-pigmented, Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming, lipolytic and gliding marine bacterium designated strain CC-AMWY-103B(T) was isolated from surface seawater collected at Kending, Taiwan. The strain shared the highest 16S rRNA gene sequence similarity of 89.4?% with Owenweeksia hongkongensis JCM 12287(T) and Brumimicrobium mesophilum YH207(T), and established a distinct phyletic lineage associated with the members of the family Cryomorphaceae. The polar lipid profile of strain CC-AMWY-103B(T) consisted of phosphatidylethanolamine, ten unidentified lipids and four unidentified aminolipids. The major fatty acids (>5?% of the total) were iso-C15?:?0, iso-C15?:?1 G, C15?:?1?5c, iso-C17?:?0 3-OH and C15?:?1?8c. The DNA G+C content was 44.2±0.3 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6) and the major polyamine was spermidine. Based on its genetic, phylogenetic, phenotypic and chemotaxonomic distinctiveness, strain CC-AMWY-103B(T) is proposed to represent a distinct member of the family Cryomorphaceae, for which the name Luteibaculum oceani gen. nov., sp. nov. is proposed; the type strain of Luteibaculum oceani is CC-AMWY-103B(T) (?=?JCM 18817(T)?=?BCRC 80551(T)). An emended description of the genus Owenweeksia is also proposed.
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Prospective randomized trial of hybrid NOTES colectomy versus conventional laparoscopic colectomy for left-sided colonic tumors.
World J Surg
PUBLISHED: 08-15-2013
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We conducted a randomized study of a laparoscopic technique for removing left-sided colon tumors that can reduce postoperative pain and other wound-related complications compared to the conventional technique. It is a novel technique of hybrid natural orifice translumenal endoscopic surgery (NOTES) colectomy (HNC) whereby laparoscopic colonic mobilization, transection, and anastomosis are performed intracorporeally. The specimen is then delivered through the anus using the transanal endoscopic operation (TEO) device, precluding the need for mini-laparotomy. We compared the short-term outcomes of patients who underwent HNC with those who underwent conventional laparoscopic colectomy (CL).
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Ultrafast time-resolved broadband fluorescence studies of the benzene-tetracyanoethylene complex: solvation, vibrational relaxation, and charge recombination dynamics.
J Phys Chem B
PUBLISHED: 08-07-2013
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The charge-transfer (CT) state relaxation dynamics of the benzene-tetracyanoethylene (BZ-TCNE) complex was studied with broadband ultrafast time-resolved fluorescence spectroscopy implemented by optical Kerr gating in three solvents of different polarities. The CT state of the BZ-TCNE complex is reached via femtosecond laser excitation, and the subsequent temporal evolutions of the fluorescence spectra were measured. Analyses of various time-dependent spectral properties revealed rapid relaxations along solvent and vibrational coordinates in competition with charge recombination (CR). By comparing the results in solvents of different polarities, we partially separated solvation and vibrational relaxation dynamics and explored the solvent-dependent CR dynamics. Time-dependent dynamic fluorescence Stokes shift (TDFSS) measurements unveiled the solvation and vibrational relaxation contributions to the observed spectral relaxation. The biphasic and slow time scales of the vibrational contributions identified in TDFSS suggested nonstatistical and hindered intramolecular vibrational-energy redistribution that can be attributed to the unique structural properties of EDA complexes. The slowest spectral relaxation of 10-15 ps identified in TDFSS was ascribed to relaxation of the BZ(+)-TCNE(-) intermolecular vibrations, which is equivalent to a structural relaxation from the initial Franck-Condon configuration to the equilibrium CT-state structure. The time scales of vibrational relaxation indicate that a fraction of the CT-state population undergoes CR reactions before complete vibrational/structural equilibrium is achieved. In carbon tetrachloride, a nonexponential temporal profile was observed and attributed to vibrational nonequilibrium CR. In dichloromethane, polar solvation greatly accelerates CR reactions, and a slower reaction-field-induced structural relaxation gives rise to a pronounced biexponential decay. The equilibrium CR time constants of the BZ-TCNE CT state are 29 ps, 150 ps, and 68 ps in dichloromethane, carbon tetrachloride, and cyclohexane, respectively.
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Pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-05-2013
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An aerobic, Gram-stain-negative, rod-shaped bacterium (designated strain CC-G9A(T)), motile by a polar-flagellum, was isolated from a hot spring water sample in Taiwan. Strain CC-G9A(T) could grow at 20-42 °C, pH 6.0-10.0 and tolerate up to 7?% (w/v) NaCl. The 16S rRNA gene sequence analysis of strain CC-G9A(T) showed pairwise sequence similarity to Pseudomonas mendocina LMG 1223(T) (97.7?%), Pseudomonas alcaligenes ATCC 14909(T) (97.8?%), Pseudomonas alcaliphila DSM 17744(T) (97.8?%), Pseudomonas toyotomiensis JCM 15604(T) (97.6?%), Pseudomonas oleovorans subsp. lubricantis DSM 21016(T) (97.6?%) and Pseudomonas argentinensis BCRC 17807(T) (97.5?%), and lower sequence similarity to other species of the genus Pseudomonas. According to DNA-DNA association analysis, the relatedness of strain CC-G9A(T) to P. mendocina BCRC 10458(T), P. alcaliphila DSM 17744(T), P. alcaligenes BCRC 11893(T), P. oleovorans subsp. lubricantis DSM 21016(T), P. argentinensis BCRC 17807(T) and P. oleovorans subsp. oleovorans BCRC 11902 was 55.1±3.1, 13.7±1.5, 14.1±1.8, 58.5±1.1, 28.9±2.0 and 28.6±1.8?%, respectively. The evolutionary trees reconstructed based on 16S rRNA, gyrB and rpoB gene sequences revealed varying phylogenetic neighbourhoods of strain CC-G9A(T) with regard to the most closely related type strains. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 64.3±1.3 mol%. The major fatty acids were C10?:?0 3-OH, C12?:?0, C12?:?0 3-OH, C16?:?0 and summed features 3 and 8 consisting of C16?:?1?7c/C16?:?1?6c and C18?:?1?7c/C18?:?1?6c, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-G9A(T) is proposed to represent a novel species within the genus Pseudomonas for which the name Pseudomonas guguanensis sp. nov. is proposed. The type strain is CC-G9A(T) (?=?BCRC 80438(T)?=?JCM 18416(T)).
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Shimia biformata sp. nov., isolated from surface seawater, and emended description of the genus Shimia Choi and Cho 2006.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-01-2013
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A novel, Gram-staining-negative, non-flagellated, oval or short-rod-shaped, strictly aerobic and non-spore-forming marine bacterium, designated strain CC-AMW-C(T), was isolated from coastal surface seawater in Kending County, Taiwan. Cells of strain CC-AMW-C(T) displayed unusual morphology and formed colourless or beige colonies on marine agar. The isolate shared pairwise 16S rRNA gene sequence similarities of 97.2 and 97.1?% with Shimia marina BCRC 80068(T) and Shimia isoporae BCRC 80085(T), respectively, and established a discrete phyletic lineage closely associated with the members of the genus Shimia. DNA-DNA hybridization values indicated <18.2?% genomic relatedness with species of the genus Shimia. The polar lipid profile of strain CC-AMW-C(T) comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, four unidentified lipids and an unidentified phospholipid. The predominant fatty acids were C18?:?1?7c and/or C18?:?1?6c (summed feature 8; 75.5?%). The DNA G+C content was 61.2 mol%. The predominant respiratory quinone was ubiquinone Q-10 and the major polyamine was cadaverine. The chemotaxonomic evidence, including extraordinary amounts of C18?:?1?7c and/or C18?:?1?6c, major polar lipids, polyamine, quinone and DNA G+C contents of CC-AMW-C(T), was in line with that of the members of the genus Shimia. Thus, strain CC-AMW-C(T) should be classified as a novel species of the genus Shimia, for which the name Shimia biformata sp. nov. is proposed. The type strain is CC-AMW-C(T) (?=?JCM 18818(T)?=?BCRC 80548(T)). An emended description of the genus Shimia is also proposed.
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Dynamic evolution of conducting nanofilament in resistive switching memories.
Nano Lett.
PUBLISHED: 07-19-2013
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Resistive random access memory (ReRAM) has been considered the most promising next-generation nonvolatile memory. In recent years, the switching behavior has been widely reported, and understanding the switching mechanism can improve the stability and scalability of devices. We designed an innovative sample structure for in situ transmission electron microscopy (TEM) to observe the formation of conductive filaments in the Pt/ZnO/Pt structure in real time. The corresponding current-voltage measurements help us to understand the switching mechanism of ZnO film. In addition, high-resolution transmission electron microscopy (HRTEM) and electron energy loss spectroscopy (EELS) have been used to identify the atomic structure and components of the filament/disrupted region, determining that the conducting paths are caused by the conglomeration of zinc atoms. The behavior of resistive switching is due to the migration of oxygen ions, leading to transformation between Zn-dominated ZnO(1-x) and ZnO.
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Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., isolated from surface seawater, and emended description of the genus Sphingomicrobium.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 07-16-2013
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Two Gram-staining-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated and non-spore-forming amylolytic marine bacterial strains, designated CC-AMZ-30M(T) and CC-AMZ-30N(T), were isolated from coastal surface seawater in Taiwan. Strain CC-AMZ-30M(T) shared pairwise 16S rRNA gene sequence similarities of 95.8, 95.0 and <94.0?% to Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. Strain CC-AMZ-30N(T) shared 97.0, 96.7, 95.0 and <95.1?% similarities to strain CC-AMZ-30M(T), Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. The common polar lipids of the two strains include a signature glycolipid (GL2), diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingoglycolipid in major amounts besides moderate-to-trace amounts of an unidentified aminolipid and several unidentified glycolipids. Both strains contained C18?:?1?7c/C18?:?1?6c, C16?:?1?7c/C16?:?1?6c, C16?:?0 and C18?:?1 2-OH as major (>5?% of the total) fatty acids. Strains CC-AMZ-30M(T) and CC-AMZ-30N(T) had DNA G+C contents of 64.2 and 65.2 mol%, respectively. The major polyamine was spermidine in strain CC-AMZ-30M(T) and triamine sym-homospermidine in strain CC-AMZ-30N(T). Both strains contained ubiquinone Q-10 as the predominant respiratory quinone. Differential phylogenetic and chemotaxonomic evidence including the presence of characteristic GL2, C18?:?1 2-OH and several other phenotypic features supported the classification of strains CC-AMZ-30M(T) and CC-AMZ-30N(T) as two novel species of the genus Sphingomicrobium, for which we propose the names Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., respectively; corresponding type strains are Sphingomicrobium marinum CC-AMZ-30M(T) (?=?JCM 18554(T)?=?BCRC 80466(T)) and Sphingomicrobium flavum CC-AMZ-30N(T) (?=?JCM 18555(T)?=?BCRC 80467(T)). An emended description of the genus Sphingomicrobium is also proposed.
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Zeaxanthin production by novel marine isolates from coastal sand of India and its antioxidant properties.
Appl. Biochem. Biotechnol.
PUBLISHED: 07-08-2013
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Zeaxanthin carotenoids are class of commercially important natural products and diverse biomolecules produced by plants and many microorganisms. Bacteria often produce a cocktail of polar and nonpolar carotenoids limiting their industrial applications. Marine members of the family Flavobacteriaceae are known to produce potential carotenoids such as astaxanthin and zeaxanthin. A few bacterial species have been reported for the predominant production zeaxanthin. Here, we report the molecular identification of the zeaxanthin as a major carotenoid produced by two novel bacteria (YUAB-SO-11 and YUAB-SO-45) isolated from sandy beaches of South West Coast of India and the effect of carbon sources on the production of zeaxanthin. The strains were identified based on the 16S rRNA gene sequencing as a member of genus Muricauda. The closest relatives of YUAB-SO-11 and YUAB-SO-45 were Muricauda aquimarina (JCM 11811(T)) (98.9 %) and Muricauda olearia (JCM 15563(T)) (99.2 %), respectively, indicating that both of these strains might represent a novel species. The highest level of zeaxanthin production was achieved (YUAB-SO-11, 1.20 ± 0.11 mg g(-1)) and (YUAB-SO-45, 1.02 ± 0.13 mg g(-1)) when cultivated in marine broth supplemented with 2 % NaCl (pH 7) and incubated at 30 °C. Addition of 0.1 M glutamic acid, an intermediate of citric acid cycle, enhanced the zeaxanthin production as 18 and 14 % by the strains YUAB-SO-11 and YUAB-SO-45 respectively. The zeaxanthin showed in vitro nitric oxide scavenging, inhibition of lipid peroxidation, and 2,2-diphenyl-1-picryl hydrazyl scavenging activities higher than the commercial zeaxanthin. The results of this study suggest that two novel strains YUAB-SO-11 and YUAB-SO-45 belonging to genus Muricauda produce zeaxanthin as a predominant carotenoid, and higher production of zeaxanthin was achieved on glutamic acid supplementation. The pigment showed good in vitro antioxidant activity, which can be exploited further for commercial applications.
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Polybutylcyanoacrylate nanoparticles for delivering hormone response element-conjugated neurotrophin-3 to the brain of intracerebral hemorrhagic rats.
Biomaterials
PUBLISHED: 06-29-2013
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Hypertensive intracerebral hemorrhage (ICH) is a rapidly evolutional pathology, inducing necrotic cell death followed by apoptosis, and alters gene expression levels in surrounding tissue of an injured brain. For ICH therapy by controlled gene release, the development of intravenously administrable delivery vectors to promote the penetration across the blood-brain barrier (BBB) is a critical challenge. To enhance transfer efficiency of genetic materials under hypoxic conditions, polybutylcyanoacrylate (PBCA) nanoparticles (NPs) were used to mediate the intracellular transport of plasmid neurotrophin-3 (NT-3) containing hormone response element (HRE) with a cytomegalovirus (cmv) promoter and to differentiate induced pluripotent stem cells (iPSCs). The differentiation ability of iPSCs to neurons was justified by various immunological stains for protein fluorescence. The effect of PBCA NP/cmvNT-3-HRE complexes on treating ICH rats was studied by immunostaining, western blotting and Nissl staining. We found that the treatments with PBCA NP/cmvNT-3-HRE complexes increased the capability of differentiating iPSCs to express NT-3, TrkC and MAP-2. Moreover, PBCA NPs could protect cmvNT-3-HRE against degradation with EcoRI/PstI and DNase I in vitro and raise the delivery across the BBB in vivo. The administration of PBCA NP/cmvNT-3-HRE complexes increased the expression of NT-3, inhibited the expression of apoptosis-inducing factor, cleaved caspase-3 and DNA fragmentation, and reduced the cell death rate after ICH in vivo. PBCA NPs are demonstrated as an appropriate delivery system for carrying cmvNT-3-HRE to the brain for ICH therapy.
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Description of Noviherbaspirillum malthae gen. nov., sp. nov., isolated from an oil-contaminated soil, and proposal to reclassify Herbaspirillum soli, Herbaspirillum aurantiacum, Herbaspirillum canariense and Herbaspirillum psychrotolerans as Noviherbaspi
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 06-07-2013
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An aerobic, Gram-negative, rod-shaped bacterium with polar flagella, strain CC-AFH3(T), was isolated from an oil-contaminated site located in Kaohsiung county, Taiwan. Strain CC-AFH3(T) grew at 20-40 °C, pH 5.0-10.0 and <2?% (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain CC-AFH3(T) showed the greatest degree of similarity to Herbaspirillum soli SUEMI10(T) (96.5?%), H. aurantiacum SUEMI08(T) (96.3?%), H. canariense SUEMI03(T) (96.0?%), H. psychrotolerans PB1(T) (95.4?%) and members of other Herbaspirillum species (94.1-95.2?%), and lower similarity to members of other genera (<94?%). Phylogenetic analyses also positioned the novel strain in the genus Herbaspirillum as an independent lineage. The major fatty acids in strain CC-AFH3(T) were C10?:?0 3-OH, C12?:?0, C14?:?0 2-OH, C16?:?0, iso-C15?:?0 3-OH, C17?:?0 cyclo, C16?:?1?7c/C16?:?1?6c and C18?:?1?7c/C18?:?1?6c. The major polar lipids of strain CC-AFH3(T) were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The predominant quinone was ubiquinone 8 (Q-8) and the DNA G+C content was 63.4 mol%. On the basis of 16S rRNA gene sequence analysis in combination with physiological and chemotaxonomic data, strain CC-AFH3(T) represents a novel species in a new genus, for which we propose the name Noviherbaspirillum malthae gen. nov., sp. nov.; the type strain of Noviherbaspirillum malthae is CC-AFH3(T) (?=?BCRC 80516(T)?=?JCM 18414(T)). We also propose the reclassification of Herbaspirillum soli, Herbaspirillum aurantiacum, Herbaspirillum canariense and Herbaspirillum psychrotolerans as Noviherbaspirillum soli comb. nov. (type strain SUEMI10(T)?=?LMG 26149(T)?=?CECT 7840(T)), Noviherbaspirillum aurantiacum comb. nov. (type strain SUEMI08(T)?=?LMG 26150(T)?=?CECT 7839(T)), Noviherbaspirillum canariense comb. nov. (type strain SUEMI03(T)?=?LMG 26151(T)?=?CECT 7838(T)) and Noviherbaspirillum psychrotolerans comb. nov. (type strain PB1(T)?=?DSM 26001(T)?=?LMG 27282(T)), respectively. An emended description of Herbaspirillum seropedicae is also presented.
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Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer.
Biomaterials
PUBLISHED: 05-20-2013
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This paper describes the fabrication of a highly efficient, non-cytotoxic drug delivery platform designed for photodynamic therapy (PDT): phospholipid-capped, protoporphyrin IX-loaded and FITC-sensitized mesoporous silica nanocarriers (Lipo-FMSNs/PpIX). After derivatization with folate on the phospholipid-capped FMSNs (denoted fa-Lipo-FMSNs/PpIX, the so-called nanoPDT system), we confirmed the nanoPDT systems selective targeting of and entry into the folic acid receptor-overexpressed HeLa cells by means of cell viability assessment and confocal microscopic analysis. The decrease in the unfavorable dark toxicity of fa-Lipo-FMSNs/PpIX enabled the delivery of high concentrations of PpIX into cells. Moreover, the cellular uptake of the nanoPDT systems was greater than that of free PpIX. Upon irradiation with visible light, the nanoPDT system generated singlet oxygen efficaciously in aqueous environments-a decisive factor affecting its therapeutic applicability in PDT, demonstrating enhanced in vitro photocytotoxicity. Furthermore, an in vivo study of subcutaneous melanoma in nude mice inoculated with B16F10 cells revealed the capability for the nanoPDT system to mitigate nearly 65% of tumor growth.
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Single-crystalline ?-Ni2Si nanowires with excellent physical properties.
Nanoscale Res Lett
PUBLISHED: 05-10-2013
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In this article, we report the synthesis of single-crystalline nickel silicide nanowires (NWs) via chemical vapor deposition method using NiCl2·6H2O as a single-source precursor. Various morphologies of ?-Ni2Si NWs were successfully acquired by controlling the growth conditions. The growth mechanism of the ?-Ni2Si NWs was thoroughly discussed and identified with microscopy studies. Field emission measurements show a low turn-on field (4.12 V/?m), and magnetic property measurements show a classic ferromagnetic characteristic, which demonstrates promising potential applications for field emitters, magnetic storage, and biological cell separation.
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Azoarcus olearius sp. nov., a nitrogen-fixing bacterium isolated from oil-contaminated soil.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 05-03-2013
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A novel nitrogen-fixing strain, designated DQS-4(T), was isolated from oil-contaminated soil in Taiwan and was characterized using a polyphasic taxonomic approach. Cells of strain DQS-4(T) stained Gram-negative, contained poly-?-hydroxybutyrate granules and were motile rods, surrounded by a thin capsule. Cells displayed a strictly aerobic type of metabolism and fixed nitrogen microaerobically. Growth occurred at 10-45 °C (optimum, 35-40 °C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-2?% NaCl (optimum, 0.5-1?%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DQS-4(T) belonged to the genus Azoarcus, and its closest neighbours were Azoarcus indigens VB32(T) and Azoarcus communis SWub3(T), with sequence similarities of 97.4 and 96.4?%, respectively. The major cellular fatty acids of strain DQS-4(T) were summed feature 3 (comprising C16?:?1?7c and/or C16?:?1?6c), C16?:?0 and C18?:?1?7c. The major cellular hydroxy fatty acid was C10?:?0 3-OH. The DNA G+C content was 64.5 mol%. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The mean level of DNA-DNA relatedness between strain DQS-4(T) and A. indigens LMG 9092(T) was 27.4?%. On the basis of the genotypic and phenotypic data, strain DQS-4(T) represents a novel species in the genus Azoarcus, for which the name Azoarcus olearius sp. nov. is proposed. The type strain is DQS-4(T) (?=?BCRC 80407(T)?=?KCTC 23918(T)?=?LMG 26893(T)).
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Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 05-03-2013
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An aerobic, Gram-stain-negative, spiral or rod-shaped, non-spore-forming, diazotrophic bacterium (strain CC-LY743(T)) was isolated from a fermentative tank in Taiwan. Strain CC-LY743(T) was able to grow at 20-37 °C and pH 6.0-8.0 and tolerated up to 3.0?% (w/v) NaCl. It was positive for nitrogen fixation, with activity of 10.6 nmol ethylene h(-1). 16S rRNA gene sequence analysis of strain CC-LY743(T) showed highest similarity to Azospirillum picis DSM 19922(T) (96.1?%), Azospirillum oryzae JCM 21588(T) (96.0?%) and Azospirillum rugosum DSM 19657(T) (96.0?%) and lower similarity (<96.0?%) to all other Azospirillum species. Highest nifH gene sequence similarities were obtained with Azospirillum brasilense BCRC 12270(T) (92.0?%), Azospirillum formosense BCRC 80273(T) (92.3?%) and A. rugosum DSM 19657(T) (91.8?%). It was positive in the rapid identification by a genus-specific primer set. The predominant quinone system was ubiquinone 10 (Q-10) and the DNA G+C content was 69.6±0.1 mol%. The major fatty acids found in strain CC-LY743(T) were n-C16?:?0, C19?:?0 cyclo ?8c, C14?:?0 3-OH/C16?:?1 iso I, C16?:?1?7c/C16?:?1?6c and C18?:?1?7c/C18?:?1?6c. Based on its phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY743(T) is considered to represent a novel species within the genus Azospirillum for which the name Azospirillum fermentarium sp. nov. is proposed. The type strain is CC-LY743(T) (?=?BCRC 80505(T)?=?JCM 18688(T)?=?LMG 27264(T)).
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Copper silicide/silicon nanowire heterostructures: in situ TEM observation of growth behaviors and electron transport properties.
Nanoscale
PUBLISHED: 05-03-2013
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Copper silicide has been studied in the applications of electronic devices and catalysts. In this study, Cu3Si/Si nanowire heterostructures were fabricated through solid state reaction in an in situ transmission electron microscope (TEM). The dynamic diffusion of the copper atoms in the growth process and the formation mechanism are characterized. We found that two dimensional stacking faults (SF) may retard the growth of Cu3Si. Due to the evidence of the block of edge-nucleation (heterogeneous) by the surface oxide, center-nucleation (homogeneous) is suggested to dominate the silicidation. Furthermore, the electrical transport properties of various silicon channel length with Cu3Si/Si heterostructure interfaces and metallic Cu3Si NWs have been investigated. The observations not only provided an alternative pathway to explore the formation mechanisms and interface properties of Cu3Si/Si, but also suggested the potential application of Cu3Si at nanoscale for future processing in nanotechnology.
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Sphingomicrobium astaxanthinifaciens sp. nov., an astaxanthin-producing glycolipid-rich bacterium isolated from surface seawater and emended description of the genus Sphingomicrobium.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 03-22-2013
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A Gram-stain-negative, rod-shaped, strictly aerobic, flagellated and non-spore-forming marine bacterium designated strain CC-AMO-30B(T) was isolated from coastal surface seawater, Taiwan. Strain CC-AMO-30B(T) synthesized astaxanthin [40 µg (g dry weight)(-1)] and formed reddish-orange-coloured colonies on marine agar (Difco 2216). The strain showed highest pairwise 16S rRNA gene sequence similarity to Sphingomicrobium lutaoense CC-TBT-3(T) (96.4%) followed by other members of the family Sphingomonadaceae (<94%) and established a discrete phyletic lineage associated with the former. The polar lipid profile constituted a remarkable number of unidentified glycolipids (GL1-8), in addition to diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and two unidentified lipids (L1-2). The major fatty acids (>5% of total fatty acids) were C(18:1)?7c/C(18:1)?6c (summed feature 8), C(16:1)?7c/C(16:1)?6c (summed feature 3), C(18:1) 2-OH, methyl C(18:1)?7c, C(17:1)?6c and C(16?:?0). DNA G+C content was 70.6%; major respiratory quinone was ubiquinone Q-10; predominant polyamine was the triamine sym-homospermidine. Chemotaxonomic evidence including characteristic glycolipid profile, presence of significant amounts of C(18:1) 2-OH and absence of typical hydroxylated fatty acids such as C(14:0) 2-OH, C(15:0) 2-OH and C(16:0) 2-OH in considerable amounts, accompanied by phylogenetic distinctiveness and several other phenotypic features support the classification of strain CC-AMO-30B(T) as a representative of a novel species within the genus Sphingomicrobium for which the name Sphingomicrobium astaxanthinifaciens sp. nov. is proposed; the type strain is CC-AMO-30B(T) (?=JCM 18551(T)?=BCRC 80465(T)).
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State vocational rehabilitation services and employment in multiple sclerosis.
Mult. Scler.
PUBLISHED: 03-21-2013
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Obtaining and maintaining suitable employment can be a significant challenge for people with multiple sclerosis (MS).
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Chiayiivirga flava gen. nov., sp. nov., a novel bacterium of the family Xanthomonadaceae isolated from an agricultural soil, and emended description of the genus Dokdonella.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 03-08-2013
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A novel Gram-reaction-negative, yellow-pigmented, aerobic, non-motile and rod-shaped bacterium designated strain CC-YHH031(T) was isolated from an agricultural soil collected at Chiayi County, Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CC-YHH031(T) formed a discrete monophyletic lineage in the family Xanthomonadaceae, sharing high pairwise sequence similarity of 93.5-95.2 and 94.8% with species of the genus Dokdonella (94.9% similarity to the type strain of the type species) and Aquimonas voraii GPTSA 20(T), respectively. The genomic DNA G+C content of strain CC-YHH031(T) was 68.6 ± 0.7 mol% and the predominant respiratory quinone was ubiquinone Q-8. Spermidine was the principal polyamine, with minor amounts of putrescine. Major fatty acids (>5% of total fatty acids) were iso-C(16:00, iso-C(15:0), C(16:1)?7c and/or C(16:1)?6c (summed feature 3), iso-C(17:1)?9c, iso-C(14:0), iso-C(11:0) and iso-C(11:0) 3-OH. The polar lipid profile of strain CC-YHH031(T) included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids (APL1-2) and four unidentified phospholipids (PL1-4). Strain CC-YHH031(T) was distinguished particularly from the type species of the genus Dokdonella (Dokdonella koreensis) by the presence of major amounts of iso-C(14:0) and summed feature 3 and minor amounts of iso-C(17:0) and by the complete absence of anteiso-C(17:0), the presence of PL1-3 and APL1-2, the absence of APL3 and the presence of putrescine in the former. On the basis of distinguishing genotypic and phenotypic evidence, strain CC-YHH031(T) is proposed to represent a novel genus and species within the family Xanthomonadaceae, for which the name Chiayiivirga flava gen. nov., sp. nov. is proposed. The type strain of Chiayiivirga flava is CC-YHH031(T) (?=BCRC 80273(T)?=DSM 24163(T)).
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Low density supercritical fluids precipitation of 9-cis and all trans-?-carotenes enriched particulates from Dunaliella salina.
J Chromatogr A
PUBLISHED: 03-06-2013
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In this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-?-carotenes from Dunaliella salina. Total concentration of 9-cis (134.7mg/g) and trans-?-carotene (204.2mg/g) was increased from 338.9mg/g of the ultrasonic extract to 859.7mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1mg/g (355.6 for 9-cis and 576.5 for trans) of total ?-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total ?-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-?-carotenes by low-density SAS was proved to be feasible and environmental benign.
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Polybutylcyanoacrylate nanoparticle-mediated neurotrophin-3 gene delivery for differentiating iPS cells into neurons.
Biomaterials
PUBLISHED: 02-27-2013
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Guided neuronal differentiation of induced pluripotent stem cells (iPSCs) with genetic regulation is an important issue in biomedical research and in clinical practice for nervous regeneration and repair. To enhance the intracellular delivery of plasmid DNA (pDNA), polybutylcyanoacrylate (PBCA) nanoparticles (NPs) were employed to mediate the transport of neurotrophin-3 (NT-3) into iPSCs. The ability of iPSCs to differentiate into neuronal lineages was shown by immunofluorescent staining, western blotting, and flow cytometry. By transmission electron microscopy, we found that PBCA NPs could efficiently grasp pDNA, thereby increasing the particle size and conferring a negative surface charge. In addition, the treatments with PBCA NP/NT-3 complexes enhanced the expression of NT-3, TrkC, NH-H, NSE, and PSD95 by differentiating iPSCs. Neurons produced from iPSCs were incapable of returning to pluripotency, demonstrating with a series of differentiation scheme for adipogenesis and osteogenesis. The pretreatment with PBCA NP/NT-3 complexes can be one of critical biotechnologies and effective delivery systems in gene transfection to accelerate the differentiation of iPSCs into neurons.
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Pseudomonas formosensis sp. nov., a gamma-proteobacteria isolated from food-waste compost in Taiwan.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 02-22-2013
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A taxonomic study was carried out on a novel aerobic bacterial strain, designated CC-CY503(T), isolated from food-waste compost in Taiwan. Cells were Gram-stain-negative short rods, motile by means of a monopolar flagellum. Strain CC-CY503(T) was able to grow at 20-50 °C and pH 6.0-10.0 and to tolerate <6% NaCl (w/v). Phylogenetic analysis of 16S rRNA gene sequences showed that this bacterium belonged to the genus Pseudomonas, with Pseudomonas pertucinogena ATCC 190(T) as the closest neighbour, sharing a sequence similarity of 97.9%. The DNA-DNA relatedness value of strain CC-CY503(T) with P. pertucinogena ATCC 190(T) was 37.8 ± 2.3%. The phylogenetic trees reconstructed based on gyrB and rpoB gene sequences supported the classification of strain CC-CY503(T) as a novel member of the genus Pseudomonas. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 63.1 ± 0.4 mol%. The major fatty acids were C(12:0), C(16:0), C(17:0) cyclo, C(19:0) cyclo ?8c and summed features 3 and 8 consisting of C(16:1)?7c/C(16:1)?6c and C(18:1)?7c/C(18:1)?6c, respectively. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of its distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-CY503(T) (?=BCRC 80437(T)?=JCM 18415(T)) is proposed to represent a novel species within the genus Pseudomonas, for which the name Pseudomonas formosensis sp. nov. is proposed.
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Endo-laparoscopic approach versus conventional open surgery in the treatment of obstructing left-sided colon cancer: long-term follow-up of a randomized trial.
Asian J Endosc Surg
PUBLISHED: 02-17-2013
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We previously conducted a randomized trial comparing the endo-laparoscopic approach (i.e. placing self-expanding metallic stents followed by laparoscopic resection) and conventional open surgery in the treatment of obstructing left-sided colon cancer. This study is a follow-up of the previous randomized trial and aims to report the long-term outcomes of the two groups.
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Vogesella fluminis sp. nov., isolated from a freshwater river, and emended description of the genus Vogesella.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 02-08-2013
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A bacterial strain designated Npb-07(T) was isolated from a freshwater river in Taiwan and characterized using a polyphasic taxonomic approach. Strain Npb-07(T) was Gram-negative, aerobic, rod-shaped and motile by means of a single polar flagellum. Growth occurred at 10-37 °C (optimum, 20-30 °C), at pH 6.0-9.0 (optimum, pH 6.0-7.0) and with 0-1 % NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-07(T) belonged to the genus Vogesella and its most closely related neighbour was Vogesella indigofera ATCC 19706(T) with sequence similarity of 98.4 %. The major fatty acids were summed feature 3 (comprising C16 : 1?7c and/or C16 : 1?6c, 44.4 %) and C16 : 0 (31.9 %). The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 65.3 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two uncharacterized aminophospholipids and an uncharacterized phospholipid. The DNA-DNA relatedness of strain Npb-07(T) with respect to V. indigofera ATCC 19706(T) was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Npb-07(T) represents a novel species in the genus Vogesella, for which the name Vogesella fluminis sp. nov. is proposed. The type strain is Npb-07(T) ( = LMG 26669(T) = BCRC 80377(T) = KCTC 23713(T)).
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Family history of chronic disease and meeting public health guidelines for physical activity: the cooper center longitudinal study.
Mayo Clin. Proc.
PUBLISHED: 01-29-2013
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We aimed to assess whether a family history of coronary heart disease, diabetes, or cancer is linked to meeting public health guidelines for health-promoting physical activity. To achieve this objective, we analyzed data on 29,513 adults who came to the Cooper Clinic (Dallas, Texas) between January 1, 1990, and December 31, 2010, for a preventive medicine visit. Patients completed a comprehensive medical survey including information on family medical history, physical activity, and other lifestyle behaviors. Bivariate and multivariate logistic regression were used to examine the relationship between having a family history of chronic disease and meeting physical activity guidelines. The results indicated that individuals with a family history of disease had reduced odds for meeting or exceeding physical activity guidelines. For example, participants with a family history of 3 diseases were 36% less likely to meet or exceed physical activity guidelines than their counterparts without a family history of disease (odds ratio, 0.64; 95% CI, 0.58-0.72), while controlling for covariates. Among this large sample of adults, those with a family history of chronic disease were less inclined to regularly engage in physical activity. Thus, targeted programs encouraging adoption and maintenance of health-promoting physical activity might be warranted, specifically targeting individuals with familial history of disease.
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Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 01-11-2013
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A Gram-positive, spore-forming, aerobic, rod-shaped, xylanolytic bacterium designated strain CC-Alfalfa-35(T) was isolated from the rhizosphere of Medicago sativa L. in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain CC-Alfalfa-35(T) was affiliated to the genus Cohnella. Strain CC-Alfalfa-35(T) shared 95.3 % pairwise 16S rRNA gene sequence similarity to the type strain of the type species of the genus Cohnella (Cohnella thermotolerans DSM 17683(T)) besides showing a similarity of 97.4-93.6 % with other recognized species of the genus Cohnella. The DNA-DNA hybridization value between CC-Alfalfa-35(T) and Cohnella thailandensis KCTC 22296(T) was 37.7 % ± 1.7 % (reciprocal value, 55.7 % ± 3.0 %). Predominant cellular fatty acids were iso-C16 : 0 and anteiso-C15 : 0. The polar lipid profile constituted diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lysyl-phosphatidylglycerol, three unidentified phospholipids and three unidentified aminophospholipids. The major respiratory quinone was MK-7 and the DNA G+C content was 58.3 mol%. Strain CC-Alfalfa-35(T) contained meso-diaminopimelic acid as the major diamino acid in the cell-wall peptidoglycan. Based on the polar lipid and fatty acid profiles, which were in line with those of C. thermotolerans DSM 17683(T), coupled with additional distinguishing genotypic, phenotypic and chemotaxonomic features, strain CC-Alfalfa-35(T) is proposed to represent a novel species within the genus Cohnella, for which the name Cohnella formosensis sp. nov. is proposed. The type strain is CC-Alfalfa-35(T) ( = JCM 18405(T) = BCRC 80428(T)).
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Effects of bone morphogenetic protein-6 on periodontal wound healing/regeneration in supraalveolar periodontal defects in dogs.
J. Clin. Periodontol.
PUBLISHED: 01-09-2013
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Application of a synthetic BMP-6 polypeptide in a rat periodontal fenestration defect model enhanced periodontal wound healing/regeneration including new bone and cementum formation. The purpose of this study was to translate the relevance of these initial observations into a discriminating large animal model.
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Periodontal repair in dogs: space-provision supports alveolar bone and cementum formation.
J. Clin. Periodontol.
PUBLISHED: 01-09-2013
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Clinical criteria for periodontal wound healing/regeneration include wound stability, space-provision and conditions for primary intention healing. However, wound stability/space-provision may be difficult to obtain in non-contained periodontal defects. The objective of this study was to; using a limited, space-providing device as a conduit, evaluate a concept of space-provision sans tissue occlusion for periodontal wound healing/regeneration.
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Microbulbifer taiwanensis sp. nov., isolated from coastal soil.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 12-09-2011
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A Gram-negative, non-spore-forming rod (CC-LN1-12(T)) was isolated from coastal soil samples of Lutao Island (Green Island), Taiwan, and its taxonomic position was studied. 16S rRNA gene sequence analysis showed that isolate CC-LN1-12(T) was grouped into the Microbulbifer cluster, with the highest similarities to Microbulbifer okinawensis ABABA23(T) (97.9?%), Microbulbifer maritimus TF-17(T) (97.7?%) and Microbulbifer donghaiensis CN85(T) (97.7?%), similarities to all other species of the genus Microbulbifer were lower than 96.8?%. The polyamine pattern contained the major compounds spermidine and cadaverine. The fatty acid profile, comprising the major fatty acids iso-C(15?:?0), iso-C(17?:?1)?9c, C(18?:?1)?7c and iso-C(11?:?0) 3-OH as the major hydroxylated fatty acid, supported the affiliation of strain CC-LN1-12(T) to the genus Microbulbifer. DNA-DNA hybridizations between strain CC-LN1-12(T) and Microbulbifer okinawensis ABABA23(T), M. donghaiensis CN85(T) and M. maritimus JCM 12187(T) resulted in relatedness values of 21.5?% (14.3?%, reciprocal analysis), 35.9?% (48.5?%, reciprocal analysis) and 48.1?% (52.1?%, reciprocal analysis), respectively. From these data, as well as from physiological and biochemical tests, strain CC-LN1-12(T) could be clearly differentiated from the most closely related species of the genus Microbulbifer. It is concluded that strain CC-LN1-12(T) represents a novel species, for which the name Microbulbifer taiwanensis sp. nov. is proposed. The type strain is CC-LN1-12(T) (?=?LMG 26125(T)?=?CCM 7856(T)).
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High-sensitivity in vivo THz transmission imaging of early human breast cancer in a subcutaneous xenograft mouse model.
Opt Express
PUBLISHED: 11-24-2011
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We performed in vivo THz transmission imaging study on a subcutaneous xenograft mouse model for early human breast cancer detection. With a THz-fiber-scanning transmission imaging system, we continuously monitored the growth of human breast cancer in mice. Our in vivo study not only indicates that THz transmission imaging can distinguish cancer from the surrounding fatty tissue, but also with a high sensitivity. Our in vivo study on the subcutaneous xenograft mouse model will encourage broad and further investigations for future early cancer screening by using THz imaging system.
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Chondrogenesis in scaffolds with surface modification of elastin and poly-L-lysine.
Colloids Surf B Biointerfaces
PUBLISHED: 11-11-2011
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A tissue engineering cartilage is of great importance in the current diarthrodial surgery. This study presents the formation of neocartilage by cultivating chondrocytes in elastin- and poly-L-lysine-modified scaffolds. The hybrid bulk biomaterials used contained polyethylene oxide, chitin, and chitosan and were fabricated by crosslinking, pre-freezing, and lyophilization. Bovine knee chondrocytes were seeded in the scaffolds and cultured in a spinner-flask bioreactor over 4 weeks. Surface elastin showed a better efficiency in the adhesion and proliferation of bovine knee chondrocytes in the scaffolds than surface poly-L-lysine. In addition, elastin-modified constructs yielded higher quantities of secreted glycosaminoglycans and produced collagen than poly-L-lysine-modified constructs. The surface morphology demonstrated a thriving chondrogenesis in the two kinds of constructs. The staining images revealed that elastin induced larger amounts of regenerated bovine knee chondrocytes, glycosaminoglycans, and type II collagen in the constructs than poly-L-lysine. Elastin- and poly-L-lysine-grafted polyethylene oxide/chitin/chitosan scaffolds are effective in producing cartilaginous components.
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A new horsehair worm, Chordodes formosanus sp. n. (Nematomorpha, Gordiida) from Hierodula mantids of Taiwan and Japan with redescription of a closely related species, Chordodes japonensis.
Zookeys
PUBLISHED: 10-27-2011
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A new species of horsehair worm, Chordodes formosanussp. n., is described and compared to a closely related species, Chordodes japonensis. Although both species possess the same six cuticular structures of areoles on the surface, the significantly longer filaments on the female crowned areoles can be used as diagnostic characters for the new species. The different taxonomic status of these two species was also confirmed after analyzing the partial cytochrome oxidase subunit I sequence, and the mantid hosts, which are respectively limited to the genus Tenodera for Chordodes japonensis and Hierodula for Chordodes formosanussp. n. In addition, the immature stages of eggs and larvae of the new species are also described and discussed in detail.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.