JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Phylogenetic relationships among orthobunyaviruses isolated from mosquitoes captured in Peru.
Vector Borne Zoonotic Dis.
PUBLISHED: 04-21-2009
Show Abstract
Hide Abstract
The Orthobunyavirus genus of the family Bunyaviridae is comprised of over 220 extremely diverse viral species. Members of this genus are often associated with acute febrile illness in animals and humans. As part of a longterm study of the ecology of arboviruses in the Amazon basin of Peru, we have isolated over 60 orthobunyaviruses from mosquitoes. The identification of many of these isolates by fluorescent antibody assay has been confounded by the lack of specificity of many available reagents. Therefore, we initiated genetic characterization, based on the S and M genomic segments, of selected viral isolates. Based on comparisons of the nucleotide sequences of the nucleocapsid gene, Wyeomyia, a virus in the Bunyamwera group, was the most related Orthobunyavirus species. Within the nonstructural S (NSs) open reading frame of the S segment, we found four conserved stop codons for the Peruvian isolates. Detailed comparisons of Bunyamwera, Simbu viruses, Group C viruses, and California viruses revealed all four of these NSs stop codons only appeared in Wyeomyia and the Peruvian isolates, and Guaroa conserved one of these stop codons. Such an apparent obliteration of the native NSs protein has not been described. Analysis of partial M segment amino acid sequence supports the conclusion that the viruses in this study are members of an uncharacterized orthobunyavirus group.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.