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Find video protocols related to scientific articles indexed in Pubmed.
A simple feature of yielding behavior of highly dense suspensions of soft micro-hydrogel particles.
Soft Matter
PUBLISHED: 10-27-2014
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The highly dense suspensions of soft micro-hydrogels with a narrow size distribution (typically ?eff > 0.9 where ?eff is the apparent volume fraction of the particle), which form a regular lattice structure, exhibit a simple feature in the yielding behavior: the yield strain ?c [ca. 2.5% and ca. 4.8% for poly(N-isopropylmethacrylamide) (PNIPMA) and poly(N-isopropylacrylamide) (PNIPA) hydrogel particles, respectively] is nearly insensitive to the cross-link concentration (cx), particle diameter (Dh), and particle concentration (c) in the limited c range examined here, and ?c is almost constant in a wide range of equilibrium shear moduli over two orders of magnitude. In addition, no appreciable difference in ?c is observed in the dense pastes with crystalline and glassy structures which are formed by mono- and bidisperse microgels, respectively. This is in contrast to a finite difference in ?c for the crystal and glass formed by the hard sphere reported by Koumakis et al. [Soft Matter, 4, 2008 (2008)]. Furthermore, the highly dense suspensions of NIPA core-NIPMA shell microgels are similar in ?c to those of NIPMA microgels. These results indicate that ?c for the highly dense suspensions of soft micro-hydrogels depends primarily on the kind of constituent polymer near the particle surface. The yield strain ?c is expected to be governed by short-range interactions such as adhesion and friction.
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[Analysis of XRD spectral characteristics of soil clay mineral in two typical cultivated soils].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-02-2014
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The present paper took black soil and chernozem, the typical cultivated soil in major grain producing area of Northeast, as the study object, and determinated the soil particle composition characteristics of two cultivated soils under the same climate and location. Then XRD was used to study the composition and difference of clay mineral in two kinds of soil and the evolutionary mechanism was explored. The results showed that the two kinds of soil particles were composed mainly of the sand, followed by clay and silt. When the particle accumulation rate reached 50%, the central particle size was in the 15-130 microm interval. Except for black soil profile of Shengli Xiang, the content of clay showed converse sequence to the central particle in two soils. Clay accumulated under upper layer (18.82%) in black soil profile while under caliche layer (17.41%) in chernozem profile. Clay content was the least in parent material horizon except in black profile of Quanyanling. Analysis of clay XRD atlas showed that the difference lied in not only the strength of diffraction peak, but also in the mineral composition. The main contents of black soil and chernozem were both 2 : 1 clay, the composition of black soil was smectite/illite mixed layer-illite-vermiculite and that of chernozem was S/I mixture-illite-montmorillonite, and both of them contained little kaolinite, chlorite, quartz and other primary mineral. This paper used XRD to determine the characteristics of clay minerals comprehensively, and analyzed two kinds of typical cultivated soil comparatively, and it was a new perspective of soil minerals study.
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High Phosphorylation Status of AKT/mTOR Signal in DESI2-Reduced Pancreatic Ductal Adenocarcinoma.
Pathol. Oncol. Res.
PUBLISHED: 06-25-2014
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Desumoylating isopeptidase 2 (DESI2) is a recently identified protein with unclear functions. In this study, a total of 132 tissue samples of pancreatic ductal adenocarcinoma and 73 samples of pancreatic normal tissues were explored to assess DESI2 expression and its implications to AKT/mTOR signal. Immunohistochemistry showed DESI2 expression is significantly decreased in cancer tissues versus normal tissues, presenting lowest level in poorly differentiated cancer. Unlike DESI2, the key factors in AKT/mTOR pathway including p-AKT, mTOR, p-mTOR and p-P70S6K present high expression in pancreatic cancer. It is notable that p-mTOR is significantly increased in DESI2-lower cancer compared with DESI2-higher cancer, although mTOR presents no difference in the two groups. The relative p-mTOR/mTOR ratio is also significantly elevated in DESI2-lower cancer. Moreover, the samples whose p-AKT and p-mTOR scores both exceed the median are obviously increased in DESI2-lower cancer compared with DESI2-higher cancer. As a downstream molecule of AKT/mTOR pathway, p-P70S6K was found to display higher level in DESI2-lower pancreatic cancer. High phosphorylation status of those proteins in DESI2-reduced pancreatic cancer indicates that there is high activity of AKT/mTOR signal in condition of DESI2 reduction, which could provide clues to reveal the implications of DESI2 in carcinogenesis.
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Implications of PPPDE1 expression in the distribution of plakoglobin and ?-catenin in pancreatic ductal adenocarcinoma.
Oncol Lett
PUBLISHED: 06-24-2014
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Human PPPDE peptidase domain-containing protein 1 (PPPDE1) is a recently identified protein; however, its exact functions remain unclear. In our previous study, the PPPDE1 protein was found to be decreased in certain cancer tissues. In the present study, a total of 96 pancreatic ductal carcinoma tissue samples and 31 normal tissues samples were assessed to investigate the distribution of plakoglobin and ?-catenin under the conditions of various PPPDE1 expression levels by means of immunohistochemistry. Generally, the staining of PPPDE1 was strong in normal tissues, but weak in cancer tissues. Plakoglobin was mainly distributed along the membrane and cytoplasm border in normal cells, but was less evident in the membranes of cancer cells. In particular, a greater percentage of cells exhibited low membrane plakoglobin expression in cancer tissue with low PPPDE1 expression (PPPDE1-low cancer) compared with that in cancer tissue with high PPPDE1 expression (PPPDE1-high cancer). The distribution of ?-catenin in normal tissues was similar to that of plakoglobin. However, ?-catenin was peculiarly prone to invade nucleus in PPPDE1-low cancer compared with PPPDE1-high cancer. Our data suggested potential links between PPPDE1 expression and the distribution of plakoglobin and ?-catenin in pancreatic ductal adenocarcinoma, providing insights into the role of PPPDE1 in the progression of pancreatic cancer.
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WNT16B from ovarian fibroblasts induces differentiation of regulatory T cells through ?-catenin signal in dendritic cells.
Int J Mol Sci
PUBLISHED: 05-16-2014
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Treatment for cancer can induce a series of secreted factors into the tumor microenvironment, which can affect cancer progression. Wingless-type MMTV (mouse mammary tumor virus) integration site 16B (WNT16B) is a new member of the WNT family and has been reported to play growth-related roles in previous studies. In this study, we found WNT16B could be expressed and secreted into the microenvironment by human ovarian fibroblasts after DNA damage-associated treatment, including chemotherapy drugs and radiation. We also demonstrated that fibroblast-derived WNT16B could result in accumulation of ?-catenin in dendritic cells and secretion of interleukin-10 (IL-10) and transforming growth factor beta (TGF-?), which contributed to the differentiation of regulatory T cells in a co-culture environment. These results shed light on the roles of WNT16B in immune regulation, especially in regard to cancer treatment.
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Puerarin inhibits angiotensin II-induced cardiac hypertrophy via the redox-sensitive ERK1/2, p38 and NF-?B pathways.
Acta Pharmacol. Sin.
PUBLISHED: 03-10-2014
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To investigate the effects of puerarin (Pue), an isoflavone derived from Kudzu roots, on angiotensin II (Ang II)-induced hypertrophy of cardiomyocytes in vivo and in vitro.
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Personalized Tacrolimus Dose Requirement by CYP3A5 but Not ABCB1 or ACE Genotyping in Both Recipient and Donor after Pediatric Liver Transplantation.
PLoS ONE
PUBLISHED: 01-01-2014
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Tacrolimus (TAC) is the backbone of an immunosuppressive drug used in most solid organ transplant recipients. A single nucleotide polymorphism (SNP) at position 6986G>A in CYP3A5 has been notably involved in the pharmacokinetic variability of TAC. It is hypothesized that CYP3A5 genotyping in patients may provide a guideline for TAC therapeutic regimen. To further evaluate the impact of CYP3A5 variants in donors and recipients, ABCB1 and ACE SNPs in recipients on TAC disposition, clinical and laboratory data were retrospectively reviewed from 90 pediatric patients with liver transplantation and their corresponding donors after 1 year of transplantation. The recipients with CYP3A5 *1/*1 or *1/*3 required more time to achieve TAC therapeutic range during the induction phase, and needed more upward dose during the late induction and the maintained phases, with lower C/D ratio, compared with those with CYP3A5 *3/*3. And donor CYP3A5 genotypes were found to impact on TAC trough concentrations after liver transplantation. No association between ABCB1 or ACE genotypes and TAC disposition post-transplantation was found. These results strongly suggest that CYP3A5 genotyping both in recipient and donor, not ABCB1 or ACE is necessary for establishing a personalized TAC dosage regimen in pediatric liver transplant patients.
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Icariin decreases the expression of APP and BACE-1 and reduces the ?-amyloid burden in an APP transgenic mouse model of Alzheimer's disease.
Int. J. Biol. Sci.
PUBLISHED: 01-01-2014
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The purpose of this study was to investigate the effects and pharmacological mechanisms of icariin, which is the main component in the traditional Chinese herb Epimedium, on ?-amyloid (A?) production in an amyloid precursor protein (APP) transgenic (Tg) mouse model of Alzheimer's disease (AD).
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[Determination of lead and isotope ratios in whole blood by inductively coupled plasma mass spectrometry].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 12-28-2013
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To establish a method to determine lead and Isotope Ratios in whole blood by inductively coupled plasma mass spectrometry (ICP-MS).
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The preparation of VEGFR1/CD3 bi-specific antibody and its specific cytotoxicity against VEGFR1-positive breast cancer cells.
Biotechnol. Appl. Biochem.
PUBLISHED: 10-06-2013
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Bi-specific antibody (BsAb) has been proved to be a very effective anti-tumor approach because of its distinctive advantages of immune-mediated cytotoxicity. In order to enhance the ability to recruit and activate T lymphocytes for tumor-specific killing, we constructed and prepared a recombinant human single chain Fv bi-specific antibody (BsAb) targeting VEGFR1 and CD3, named VEGFR1/CD3 BsAb. The VEGFR1/CD3 BsAb was expressed in CHO-K1 cells and purified by Ni-NTA affinity chromatography. The CD3 and VEGFR1 binding activity of VEGFR1/CD3 BsAb was confirmed by flow cytometry. T lymphocyte activation and proliferation induced by VEGFR1/CD3 BsAb were also demonstrated in vitro. Notably, our VEGFR1/CD3 BsAb presented powerful and specific killing effect against VEGFR1-positive human breast cancer cell MDA-MB-231 and MDA-MB-435 through activating T lymphocyte at very low concentration, indicating it will be a valuable antibody drug for treatment of VEGFR1-positive cancers in future. This article is protected by copyright. All rights reserved.
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Potent antitumor actions of the new antibiotic boningmycin through induction of apoptosis and cellular senescence.
Anticancer Drugs
PUBLISHED: 02-09-2011
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Boningmycin, a new antibiotic of the bleomycin family, is isolated from the fermentation broth of Streptomyces verticillus var. pingyangensis n.sp. This study aimed to evaluate its antitumor actions and mechanism. The results showed that boningmycin exhibited potent inhibitory effects on several human solid tumor cells and that it was stronger than bleomycin. The administration of boningmycin inhibited the growth of human hepatoma HepG2 xenografts in nude mice, with more efficacy than that of bleomycin. Boningmycin led to an increase of the reactive oxygen species involving iron and caused G2/M phase accumulation in the HepG2 and human breast cancer MCF-7 cells. Two types of cell death, apoptosis and senescence, were detected after exposure to boningmycin. The accumulation of sub-G1 phase cells, an index of apoptosis, and the activation of caspase apoptotic pathways were detected after treatment with higher concentrations of boningmycin. Low concentrations of boningmycin led to a senescent phenotype with an increase in senescence-associated ?-galactosidase activity and the time-dependent increase of p21, p27, and p53 expressions from 48 to120 h. Taken together, the results showed that boningmycin exhibits potent antitumor actions through the induction of apoptosis and cellular senescence.
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Induction of programmed cell death in Arabidopsis and rice by single-wall carbon nanotubes.
Am. J. Bot.
PUBLISHED: 09-10-2010
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• Premise of the study: Single-walled carbon nanotubes (SWCNTs) have many unique structural and mechanical properties. Their potential applications, especially in biomedical engineering and medical chemistry, have been increasing in recent years, but the toxicological impact of nanoparticles has rarely been studied in plants. • Methods: We exposed Arabidopsis and rice leaf protoplasts to SWCNTs and examined cell viability, DNA damage, reactive oxygen species generation, and related gene expression. We also tested the effects of nanoparticles on Arabidopsis leaves after injecting a SWCNT solution. EM-TUNEL (electron-microscopic terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) and a cerium chloride staining method were used. • Key results: SWCNTs caused adverse cellular responses including cell aggregation, chromatin condensation along with a TUNEL-positive reaction, plasma membrane deposition, and H(2)O(2) accumulation. The effect of SWCNTs on the survival of cells was dose dependent, with 25 ?g/mL inducing 25% cell death in 6 h. In contrast, activated carbon, which is not a nano-sized carbon particle, did not induce cell death even 24 h after treatments. The data indicated that the nano-size of the particle is a critical factor for toxicity. Moreover, endocytosis-like structures with cerium chloride deposits formed after SWCNT treatment, suggesting a possible pathway for nanoparticles to traverse the cell membrane. • Conclusions: Consequently, SWCNTs have an adverse effect on protoplasts and leaves through oxidative stress, leading to a certain amount of programmed cell death. Although nanomaterials have great advantages in many respects, the benefits and side effects still need to be assessed carefully.
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[Enhanced thymidine kinase gene vector and its killing effect on nasopharyngeal carcinoma in vitro and in vivo].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 07-27-2010
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To construct a modified and enhanced thymidine kinase (TK) vector regulated by human telomerase catalytic subunit promoter (hTERT) promoter and cytomegalovirus (CMV) enhancer and its killing effect on nasopharyngeal carcinoma in vitro and in vivo and its safety in vivo.
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Targeted gene therapy of nasopharyngeal cancer in vitro and in vivo by enhanced thymidine kinase expression driven by human TERT promoter and CMV enhancer.
J. Exp. Clin. Cancer Res.
PUBLISHED: 04-14-2010
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To explore the therapeutic effects of thymidine kinase (TK) expressed by enhanced vector pGL3-basic- hTERTp-TK-EGFP-CMV driven by human telomerase reverse transcriptase promoter (hTERTp) as well as cytomegalovirus immediate early promoter enhancer (CMV).
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The role of entecavir in preventing hepatitis B recurrence after liver transplantation.
J Dig Dis
PUBLISHED: 11-13-2009
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Although hepatitis B recurrence after liver transplantation has been reduced to 0%-10% since the application of the combination therapy of hepatitis B immunoglobulin (HBIG) and lamivudine, the viral mutation resistance of lamivudine is still an obstacle to the outcome of liver transplantation. Here we evaluate the role of entecavir in preventing hepatitis B recurrence after liver transplantation.
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An insight into the protection of rat liver against ischemia/reperfusion injury by 2-selenium-bridged beta-cyclodextrin.
Hepatol. Res.
PUBLISHED: 07-13-2009
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Aim: The reperfusion following liver ischemia results in the damage and apoptosis of hepatocytes. The aim of this study was to investigate the possible effects and mechanism of a new synthesized glutathione peroxidase (GPX) mimic, 2-selenium-bridged beta-cyclodextrin (2-SeCD), on rat liver ischemia-reperfusion (I/R) injury. Methods: Male Wistar rats (n = 32) were randomly divided into four groups: I. sham-operated group, II. I/R group, III. I/R +2-SeCD group, IV. I/R + Ebselen group. Hepatic I/R was administered by 90 min of ischemia and 12 h of reperfusion. Liver tissues were collected at the end of reperfusion period for measurement of various biochemical parameters. Results: The serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) activity and tissue malondialdehyde, myeloperoxidase levels were increased in I/R group, while the increase was significantly reduced by 2-SeCD treatment. The glutathione level, depressed by I/R, was elevated back to normal levels by treatment with 2-SeCD. Severe hepatic damage were observed by light and transmission electron microscopy whilst pretreatment with 2-SeCD resulted in tissue and cellular preservation. Furthermore, 2-SeCD reduced cytochrome c release from mitochondria and subsequent DNA fragmentation by regulating Bcl-2/Bax expression ratio. Results suggested that 2-SeCD was more effective than ebselen in the reversal of the alteration in tissue structural and biochemical parameters caused by I/R injury. Conclusion: 2-selenium-bridged beta-cyclodextrin playes an important role in the protection of liver against I/R injury and this treatment may be a novel pharmacological agent for liver surgery.
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[Evaluation of the capacity of personal protection on poison emergency items in Chinese disease control and prevention institutes].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 06-19-2009
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To analyze the capacity of personal protection on poison emergency items in Chinese disease control and prevention institutes.
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The effect of a cholesterol liquid crystalline structure on osteoblast cell behavior.
Biomed Mater
PUBLISHED: 03-04-2009
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To investigate the effect of a liquid crystalline structure on cell behavior, polymethylsiloxane-graft-(10-cholesteryloxydecanol) was specially designed to get a thermotropic liquid crystalline polymer. Results of Fourier transform infrared (FT-IR) spectroscopy, proton nuclear magnetic resonance (1H-NMR) spectroscopy and gel permeation chromatography (GPC) indicated that cholesterol was successfully covalently grafted onto polymethylhydrosiloxane via decamethylene flexible spacer. Differential scanning calorimetry (DSC) and polarized optical microscopy (POM) investigations revealed that the copolymer with 44.9% mesogenic unit showed obvious thermotropic liquid crystalline transition at about 124.9 degrees C. Polymer films were prepared by spin coating on clean glass plates from 5 mg ml(-1) toluene solutions of the copolymers. The POM investigation indicated that while the unannealed films (SC15, SC45) showed no liquid crystalline structure, the films which were annealed in vacuo at 140 degrees C for 9 h and then quenched to room temperature (SC15C, SC45C) formed discrete island-like liquid crystalline and continuous liquid crystalline structures, respectively. Osteoblast cells (MC3T3) were chosen to test the cell behavior of annealed and unannealed films. In comparison to unannealed films, the annealed films with a cholesterol liquid crystalline structure could promote osteoblast cell attachment and growth significantly.
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Diagnostic value of endometrial thickness determined by transvaginal sonography in infertile women with endometrial polyps.
Chin. Med. J.
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Endometrial polyps (EPs) occur in approximately 34.9% of infertile women. Transvaginal sonography (TVS) is a routine, non-invasive component of fertility evaluation. Most ultrasonographic studies of EPs have focused on abnormal uterine bleeding; few have assessed EPs in infertile women. Furthermore, no studies have explored endometrial thickness and its correlation with EPs in infertile women. This study aimed to assess transvaginal sonographic assessment of endometrial thickness and its value in diagnosis and prediction of EPs in infertile women.
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[Risk factors for excessive bleeding in patients undergoing open heart surgery with cardiopulmonary bypass].
Zhonghua Yi Xue Za Zhi
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To evaluate the pre- and intraoperative risk factors associated with excessive bleeding during the perioperative period in adult patients undergoing open heart surgery with cardiopulmonary bypass (CPB).
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PinX1 regulation of telomerase activity and apoptosis in nasopharyngeal carcinoma cells.
J. Exp. Clin. Cancer Res.
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Human interacting protein X1 (PinX1) has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, however, its function in inhibiting telomerase activity of tumor cells is not well documented. Here we show that PinX1 is essential for down-regulation telomerase activity of nasopharyngeal carcinoma.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.