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Find video protocols related to scientific articles indexed in Pubmed.
Facile microwave-assisted synthesis of Klockmannite CuSe nanosheets and their exceptional electrical properties.
Sci Rep
PUBLISHED: 08-08-2014
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Klockmannite copper selenide nanosheets (CuSe NSs) are synthesized by a facile microwave-assisted method and fully characterized. The nanosheets have smooth surface and hexagonal shape. The lateral size is 200-500 nm × 400-800 nm and the thickness is 55 ± 20 nm. The current-voltage characteristics of CuSe NS films show unique Ohmic and high-conducting behaviors, comparable to the thermally-deposited gold electrode. The high electrical conductivity of CuSe NSs implies their promising applications in printed electronics and nanodevices. Moreover, the local electrical variation is observed, for the first time, within an individual CuSe NS at low bias voltages (0.1 ~ 3 V) by conductive atomic force microscopy (C-AFM). This is ascribed to the quantum size effect of NS and the presence of Schottky barrier. In addition, the influence of the molar ratio of Cu(2+)/SeO2, reaction temperature, and reaction time on the growth of CuSe NSs is explored. The template effect of oleylamine and the intrinsic crystal nature of CuSe NS are proposed to account for the growth of hexagonal CuSe NSs.
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Silicon dioxide particles deposited in vessels and cartilage of the femoral head.
Yonsei Med. J.
PUBLISHED: 07-23-2014
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Silicosis had been considered for decades as an illness with manifestations of lung fibrosis due to inhalation of overconcentrated SiO? dust. To the best of our knowledge, studies have yet to report SiO? deposits in any other tissues and organs. In the present case, while performing bilateral artificial total hip arthroplasty for one patient, we found that the articular cartilage of the bilateral femoral head was black. Therefore, specimens thereof were sent for pathological examination. Pathological examination (immunohistochemistry) and polarized light microscopy revealed the presence of considerable brown, acicular, rhombic, and crumb-like crystals. The crystals were mainly composed of SiO?. SiO? could deposit in vessels and femoral head cartilage via blood circulation.
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Effects of tetraspanin CD151 inhibition on A549 human lung adenocarcinoma cells.
Mol Med Rep
PUBLISHED: 06-26-2014
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Tetraspanin protein CD151 is overexpressed in a wide variety of cancer types, including lung cancer, and is closely associated with metastasis and poor prognosis of carcinoma. To investigate whether knockdown of CD151 expression can inhibit the malignant biological behavior of lung adenocarcinoma (LAC), RNA interference technology (RNAi) was used to silence CD151 expression in the A549 LAC cell line. Specific small interfering RNA (siRNA) for targeting human endogenous CD151 were delivered into A549 cells in order to examine the effects on cell proliferation, survival, migration, invasion and colony formation. The expression levels of CD151 were assayed by western blotting, proliferation was evaluated by MTT method and apoptosis was determined by flow cytometry. The invasive and metastatic ability of A549 cells was investigated by wound healing and Boyden chamber assays. Colony formation analysis was used to determine the A549 cell growth properties. Finally, the expression of phosphorylated FAK, PI3K?AKT, MEK?Erk1/2, MMPs, and VEGF was detected by western blotting. The results demonstrated that CD151?siRNA significantly decreased the expression level of CD151 in A549 cells. Reduced CD151 expression in A549 cells lead to the inhibition of cellular proliferation, migration, invasion and colony formation and an enhancement of apoptosis. Furthermore, the expression of tumor development?related proteins, including FAK, PI3K?AKT, MEK?ERK1/2MAPK as well as the expression of MMP9 and VEGF, were restrained. Taken together, the present study has shown that CD151 expression is essential for LAC progression. Thus, knockdown CD151 expression by targeted siRNA could inhibit the related downstream intercellular signaling pathways, and this may provide a novel gene therapy for patients with LAC.
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Serum uric acid levels are associated with polymorphisms in the SLC2A9, SF1, and GCKR genes in a Chinese population.
Acta Pharmacol. Sin.
PUBLISHED: 05-22-2014
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Genome-wide association studies have identified several novel loci associated with serum uric acid concentrations in individuals of European descent. In the current study, we aimed to evaluate the associations between these loci and serum uric acid concentrations in a Chinese population.
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[Long-term efficacy and safety of telbivudine as monotherapy and as combination therapy with adefovir dipivoxil in HBeAg-positive chronic hepatitis B patients].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 05-15-2014
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To prospectively observe the long-term antiviral efficacy and safety of telbivudine (LDT) administered as a monotherapy and as a combination therapy with adefovir dipivoxil (ADV) in patients diagnosed with chronic hepatitis B (CHB) and positivity for hepatitis B e antigen (HBeAg).
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Schisantherin A suppresses osteoclast formation and wear particle-induced osteolysis via modulating RANKL signaling pathways.
Biochem. Biophys. Res. Commun.
PUBLISHED: 04-20-2014
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Receptor activator of NF-?B ligand (RANKL) plays critical role in osteoclastogenesis. Targeting RANKL signaling pathways has been a promising strategy for treating osteoclast related bone diseases such as osteoporosis and aseptic prosthetic loosening. Schisantherin A (SA), a dibenzocyclooctadiene lignan isolated from the fruit of Schisandra sphenanthera, has been used as an antitussive, tonic, and sedative agent, but its effect on osteoclasts has been hitherto unknown. In the present study, SA was found to inhibit RANKL-induced osteoclast formation and bone resorption. The osteoclastic specific marker genes induced by RANKL including c-Src, SA inhibited OSCAR, cathepsin K and TRAP in a dose dependent manner. Further signal transduction studies revealed that SA down-regulate RANKL-induced nuclear factor-kappaB (NF-?B) signaling activation by suppressing the phosphorylation and degradation of I?B?, and subsequently preventing the NF-?B transcriptional activity. Moreover, SA also decreased the RANKL-induced MAPKs signaling pathway, including JNK and ERK1/2 posphorylation while had no obvious effects on p38 activation. Finally, SA suppressed the NF-?B and MAPKs subsequent gene expression of NFATc1 and c-Fos. In vivo studies, SA inhibited osteoclast function and exhibited bone protection effect in wear-particle-induced bone erosion model. Taken together, SA could attenuate osteoclast formation and wear particle-induced osteolysis by mediating RANKL signaling pathways. These data indicated that SA is a promising therapeutic natural compound for the treatment of osteoclast-related prosthesis loosening.
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Inhibitory effect of rape pollen supercritical CO2 fluid extract against testosterone-induced benign prostatic hyperplasia in rats.
Exp Ther Med
PUBLISHED: 03-25-2014
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Benign prostatic hyperplasia (BPH) can lead to lower urinary tract symptoms. Rape pollen is an apicultural product that is composed of nutritionally valuable and biologically active substances. The aim of the present study was to investigate the protective effect of rape pollen supercritical CO2 fluid extract (SFE-CO2) in BPH development using a testosterone-induced BPH rat model. BPH was induced in the experimental groups by daily subcutaneous injections of testosterone for a period of 30 days. Rape pollen SFE-CO2 was administered daily by oral gavage concurrently with the testosterone injections. Animals were sacrificed at the scheduled termination and the prostates were weighed and subjected to histopathological examination. Testosterone, dihydrotestosterone (DHT), 5?-reductase and cyclooxygenase-2 (COX-2) levels were also measured. BPH-induced animals exhibited an increase in prostate weight with increased testosterone, DHT, 5?-reductase and COX-2 expression levels. However, rape pollen SFE-CO2 treatment resulted in significant reductions in the prostate index and testosterone, DHT, 5?-reductase and COX-2 levels compared with those in BPH-induced animals. Histopathological examination also demonstrated that rape pollen SFE-CO2 treatment suppressed testosterone-induced BPH. These observations indicate that rape pollen SFE-CO2 inhibits the development of BPH in rats and these effects are closely associated with reductions in DHT, 5?-reductase and COX-2 levels. Therefore, the results of the present study clearly indicate that rape pollen SFE-CO2 extract may be a useful agent in BPH treatment.
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Expression of Sapium sebiferum (L.) Roxb stearoyl-acyl carrier protein desaturase in Escherichia coli.
Protein Expr. Purif.
PUBLISHED: 03-18-2014
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Sapium sebiferum (L.) Roxb is one of the most important woody oil trees in China. Stearoyl-acyl carrier protein desaturase (SAD) is a dehydrogenase enzyme that plays the principal role in the transformation of saturated fatty acids into unsaturated fatty acids in oil plants. In this study, the full-length cDNA encoding S.sebiferum (L.) Roxb SAD (SsSAD) was amplified by RT-PCR and cloned into the cold-shock-inducible expression vector, pCold TF. The recombinant prokaryotic expression plasmid was then transformed into the BL21 star (DE3) strain of Escherichia coli (E. coli). Expression of the fusion protein was induced at low temperature in the transformed bacteria using isopropylthio-d-galactoside (IPTG). A recombinant protein with a molecular mass of 101kDa was highly expressed in transformed E. coli, and present in both the supernatant and pellet fractions of bacterial lysates. The supernatant was further purified by affinity chromatography and subjected to Western blot analysis. Ninety percent of the recombinant protein was present in the soluble fraction, confirming that the expression and purification strategy was successful in producing high-quality SsSAD protein. An enzyme activity assay was used to verify that the recombinant SsSAD had stearoyl-ACP desaturase activity. These data lay a foundation for further investigation of the structure and function of SsSAD.
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Huge giant cell tumor of the sacrum: A case report.
Oncol Lett
PUBLISHED: 01-17-2014
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The current report describes the case of a 29-year-old female with a sacral giant cell tumor (GCT) during pregnancy. Originally, the patient presented with severe pain in the lumbosacral region, radiating posterolaterally from the lumbar spine into the bilateral thigh and subsequently, into the bilateral crus posterolaterally. Plain X-rays, computed tomography and magnetic resonance imaging showed osteolytic destruction of the sacrococcygeal bones and a huge soft-tissue mass with features of a chordoma. The patient underwent a partial en bloc sacrectomy (partial S1 and completely below) and curettage for tumors located at the sacroiliac joint and underlying left ilium, with bilateral internal iliac arteries ligated to control intraoperative hemorrhage. The patient's bilateral S2 nerve roots were killed. The diagnosis of conventional GCT was determined based on the histopathological examination of the resected specimen. Urinary and bowel functions were recovered by exercising.
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Dysplasia epiphysealis hemimelica with involvement of the distal tibial epiphysis and talus: recurrence of a case and literature review.
J Foot Ankle Surg
PUBLISHED: 01-08-2014
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Dysplasia epiphysealis hemimelica is a rare developmental disorder with unknown etiology affecting epiphysis mostly in childhood. Dysplasia epiphysealis hemimelica is usually considered an intra-articular variant of an osteochondroma owing to its presenting symptoms and histologic findings. Surgical treatment is mandatory when symptoms such as pain, joint impingement, or deformity are present and will yield good results when the mass is juxta-articular or extra-articular. In those cases in which the mass is intra-articular and surgical intervention could affect the growth of the epiphysis, surgical treatment should be carefully evaluated and considered. A case of recurrence of dysplasia epiphysealis hemimelica located in the distal tibial epiphysis and talus that was successfully treated with surgery is presented.
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Differentiation of Th subsets inhibited by nonstructural proteins of respiratory syncytial virus is mediated by ubiquitination.
PLoS ONE
PUBLISHED: 01-01-2014
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Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, constitutes an important risk factor for the development of subsequent asthma. However, the mechanism underlying RSV-induced asthma is poorly understood. Viral non-structural proteins NS1 and NS2 are critically required for RSV virulence; they strongly suppress IFN-mediated innate immunity of the host cells. In order to understand the effects of NS1 and NS2 on differentiation of Th subsets, we constructed lentiviral vectors of NS1 or NS2 to infect 16 HBE and analyzed the expression of HLA-DR, CD80 and CD86 and differentiation of Th1, Th2 and Th17 by Flow Cytometric Analysis and real-time PCR. The results showed that NS1 inhibited expression of HLA-DR, CD80 and CD86 and differentiation of Th1, Th2 and Th17 lymphocytes, which could be reversed by deleting elongin C binding domain. NS2 inhibited the differentiation of Th2 and Th17, which was reversed by proteasome inhibitors of PS-341. Our results indicated that NS1 inhibited the differentiation of T lymphocytes through its mono-ubiquitination to interacted proteins, while NS2 inhibited differentiation of Th2 and Th17 through ubiquitin-proteasome pathway, which may be related with the susceptibility to asthma after RSV infection.
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Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells.
PLoS ONE
PUBLISHED: 01-01-2014
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Endothelin-1 (ET-1)/endothelin A receptor (ETAR) signaling is important for osteosarcoma (OS) progression. Monoclonal antibodies (mAbs) targeting ganglioside GD2 reportedly inhibit tumor cell viability independent of the immune system. A recent study suggests that ganglioside GD2 may play an important role in OS progression. In the present study, we for the first time explored the effects of anti-GD2 mAb alone or in combination with ETAR antagonist on OS cell invasiveness and viability. Human OS cell lines Saos-2, MG-63 and SJSA-1 were treated with control IgG (PK136 mAb, 50 µg/mL), anti-GD2 14G2a mAb (50 µg/mL), selective ETAR antagonist BQ123 (5 µM), or 14G2a (50 µg/mL)+BQ123 (5 µM). Cells with knockdown of ETAR (ETAR-shRNA) with or without 14G2a mAb treatment were also tested. Cells treated with selective phosphatidylinositide 3-kinase (PI3K) inhibitor BKM120 (50 µM) were used as a positive control. Our results showed that BQ123, ETAR-shRNA and 14G2a mAb individually decreased cell invasion and viability, matrix metalloproteinase-2 (MMP-2) expression and activity, PI3k activity, and phosphorylation at serine 473 (ser473) of Akt in OS cells. 14G2a mAb in combination with BQ123 or ETAR-shRNA showed significantly stronger inhibitory effects compared with each individual treatment. In all three cell lines tested, 14G2a mAb in combination with BQ123 showed the strongest inhibitory effects. In conclusion, we provide the first in vitro evidence that anti-ganglioside GD2 14G2a mAb effectively inhibits cell invasiveness, MMP-2 expression and activity, and cell viability in human OS cells. ETAR antagonist BQ123 significantly enhances the inhibitory effects of 14G2a mAb, likely mainly through inhibiting the PI3K/Akt pathway. This study adds novel insights into OS treatment, which will serve as a solid basis for future in vivo studies on the effects of combined treatment of OS with anti-ganglioside GD2 mAbs and ETAR antagonists.
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[Quality of life in children with short stature: an analysis using PedsQL].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 10-18-2013
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To study the differences in quality of life and psychosocial function between children with short stature and children with normal stature.
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[Respiratory physicians knowledge, attitude and practice of tobacco control and their smoking status in the city of Chongqing].
Zhonghua Jie He He Hu Xi Za Zhi
PUBLISHED: 08-16-2013
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To investigate the smoking status, knowledge of smoking hazards, attitude of tobacco control and skill of assisting smoking cessation in respiratory physicians in the city of Chongqing and therefore to provide references for their further participation in social tobacco control.
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[Preparation and performance investigation of Trichoderma viride-modified corn stalk as sorbent materials for oil spills].
Huan Jing Ke Xue
PUBLISHED: 06-27-2013
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This work aims at preparing oil spill sorbent (TCS, Trichoderma viride-modified corn stalk) through solid-state fermentation of corn stalk by Trichoderma viride. Single-factor experiments, including the effect of modification time, solid-liquid ratio of modification and modification temperature, and adsorption experiments simulating oil spill condition, were carried out. The results indicated that the maximum oil adsorption of TCS, 13.84 g x g(-1), could be obtained under the conditions of 6 days of modification, with a solid-liquid ratio of 1:4 and a modification temperature of 25 degrees C. This oil absorption was 110.33% of that of the raw material (RCS, Raw Corn Stalk). Comparing RCS and TCS by means of Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and X-ray Diffraction (XRD), the results separately showed that TCS had rougher surface, lower cellulose content and higher instability, which explains the increase of oil absorption. Also, the component analysis indicated that bio-modification could reduce the contents of celluloses and hemicelluloses from corn stalk. Besides, sorption kinetics and oil retention performance test showed that, TCS, which could reach adsorption equilibrium after 1 h of 80 r x min(-1) oscillating, had fast oil adsorption rate, and it also had good oil retention performance, which could keep 74. 87% of the initial adsorption rate when trickling 10 min after reaching adsorption equilibrium.
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The role of positive charges on G-quadruplex binding small molecules: learning from bisaryldiketene derivatives.
Biochim. Biophys. Acta
PUBLISHED: 05-27-2013
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G-quadruplexes are promising therapeutic targets for small molecules. In general, the introduction of steady positive charges through the in situ alkylation of nitrogen atoms within potential G-quadruplex ligands can significantly improve their quadruplex binding and stabilization abilities. However, our previous studies on bisaryldiketene derivatives showed that the derivative M4, whose central piperidone moiety is quaternized, exhibits a poor G-quadruplex stabilization ability.
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MicroRNA-376c inhibits cell proliferation and invasion in osteosarcoma by targeting to transforming growth factor-alpha.
DNA Cell Biol.
PUBLISHED: 04-30-2013
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MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-376c expression level was decreased while transforming growth factor-alpha (TGFA) mRNA expression levels were increased in osteosarcoma tissues and cell lines, and we identified TGFA as a novel direct target of miR-376c. Overexpression of miR-376c suppressed TGFA expression and the expression of its downstream signaling molecule such as epidermal growth factor receptor, and attenuated cell proliferation and invasion. Forced expression of TGFA could partly rescue the inhibitory effect of miR-376c in the cells. Taken together, these findings will shed light on the role and mechanism of miR-376c in regulating osteosarcoma cell growth via miR-376c/TGFA axis, and miR-376c may serve as a potential therapeutic target in osteosarcoma in the future.
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Molecular switch for cold acclimation -- anatomy of the cold-inducible promoter in plants.
Biochemistry Mosc.
PUBLISHED: 04-18-2013
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In plants, a promoter is essential to drive the transcription and expression of genes under stress conditions. The cold-regulated promoter is an important molecular switch involved in transcriptional regulation of a dynamic network of genes associated with cold acclimation processes. However, the structure and functions of the cold-regulated promoter are ambiguous. In this review, we first describe the common type and structures of the cold-regulated promoter, such as the core promoter and transcription factor binding sites, and then discuss the synergistic actions of promoter elements and cold-regulated genes. We also describe the transcriptional responses and cross-talk among cold-regulated genes in the ICE-CBF-COR cold-response pathway. Many stress-inducible genes are known to be regulated by endogenous abscisic acid (ABA), which accumulates during osmotic and cold stress. We discuss the regulation of promoters of cold-inducible genes in ABA-dependent and ABA-independent regulatory systems. We also describe the cross-talk among gene networks regulated by different cis-acting regulatory elements. Finally, we propose potential further research on, and practical applications of, the cold-regulated promoter.
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Expression of NF-?B and osteopontin of synovial fluid of patients with knee osteoarthritis.
Asian Pac J Trop Med
PUBLISHED: 03-10-2013
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To explore the significance of osteopontin and nuclear factor ?B (NF-?B) expression in patients with knee osteoarthritis.
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Risk factors for hematological toxicity of chemotherapy for bone and soft tissue sarcoma.
Oncol Lett
PUBLISHED: 02-28-2013
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The aim of this study was to assess chemotherapy treatment characteristics, neutropenic event occurrence and related risk factors in bone and soft tissue sarcoma patients in China. Knowledge of such risk factors aids healthcare providers in focusing resources on those who are at most risk and targeting prophylactic colony-stimulating factors (CSFs) for those patients. The study included 113 children and adults with different types of sarcoma who had been treated with neoadjuvant chemotherapy for bone and soft tissue sarcoma in order to identify risk factors for hematological toxicity of chemotherapy for bone and soft tissue sarcoma. Risk factors were determined using multivariate logistic regression analysis. Factors such as age <20 years, Karnofsky Performance Status Scale (KPS) score <60, malnutrition, number of previous chemotherapies >3 and combination therapy with >3 drugs were significantly associated with occurrence of grade III/IV neutropenia, suggestive of severe bone marrow suppression. Patients with such characteristics are at most risk of severe bone marrow suppression, and preventing discontinuation of treatment would be valuable for treating patients more effectively.
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Development of a Streptococcus gordonii vaccine strain expressing Schistosoma japonicum Sj-F1 and evaluation of using this strain for intranasal immunization in mice.
Parasitol. Res.
PUBLISHED: 01-28-2013
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Schistosomiasis is a worldwide parasitic disease. Currently, chemotherapy is the main effective method to treat schistosomiasis; however, it does not prevent reinfection. No effective vaccine is currently available to prevent schistosomiasis. Sj-F1 (GenBank accession number AY261995) is a novel gene that was discovered through screening adult Schistosoma japonicum worm cDNA library with female S. japonicum antigen-immunized sera. Streptococcus gordonii, a normal inhabitant of the human oral cavity, has been a prime candidate in recent investigations toward developing a live oral vaccine vector. One of the approaches for the surface expression of heterologous antigens in S. gordonii is to surface-localize them with the M6 protein from Streptococcus pyogenes. Here, we develop a recombinant S. gordonii strain that expresses the M6-Sj-F1 fusion protein on the bacterial surface. Intranasal immunization in mice with such M6-Sj-F1-expressing S. gordonii bacteria induced strong serum IgG, serum IgA, and saliva IgA against Sj-F1. The results of protective immunity against a challenge with cercariae of S. japonicum showed statistically significant protection following this treatment, with a worm reduction rate of 21.45% and an egg reduction rate of 34.77%. Our data indicate that the described M6-Sj-F1-expressing S. gordonii is highly immunogenic and can partially protect mice from challenge infection with S. japonicum. Intranasal immunization with recombinant S. gordonii may be an alternative to developing a novel S. japonicum vaccine in a safe, effective, and feasible way.
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Application of cellulase for the modification of corn stalk: leading to oil sorption.
Bioresour. Technol.
PUBLISHED: 01-21-2013
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In this work, a new biotechnological procedure was developed using cellulase as a modifier to produce oil sorbent from corn stalk (CMCS). Cellulase treatment of raw corn stalk (RCS) with enzyme loading of 100 U/g at 45°C for 6h resulted in high oil sorption capacity. The sorption capacities of vegetable oil, diesel and crude oil by CMCS were 18.47, 16.15 and 27.23 g/g, respectively, which were found to be much higher than RCS. XRD, BET and SEM were applied to characterize RCS and CMCS. The effects of sorbent dose (0.1-0.5 g), initial oil amount (5-30 g), and the sorption kinetics were also studied. This work demonstrated that corn stalk modified by cellulase is an efficient and environment-friendly biosorbent for the removal of spilled oil.
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Management of combined bone defect and limb-length discrepancy after tibial chronic osteomyelitis.
Orthopedics
PUBLISHED: 08-06-2011
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Bone defects and leg shortening due to chronic osteomyelitis are rare yet challenging problems to treat. The aim of this study was to summarize our experience with a distraction osteogenesis technique performed with an external fixator for these conditions. Twenty-three consecutive patients with tibial bone defects and limb-length discrepancy caused by osteomyelitis were treated from January 1994 to January 2009. Thirteen boys and 10 girls had a mean age of 12.2 years (range, 8-16 years). Twenty of the 23 patients (87.0%) had undergone an unsuccessful bone grafting procedure. Mean amount of bone defect was 3.6 cm (range, 1.5-6.2 cm) as measured on plain radiographs. Mean leg-length discrepancy was 4.0 cm (range, 0-8.0 cm). All patients were followed for a mean of 116 months (range, 31-182 months). Mean external fixation index was 48.0 days/cm (range, 40.7-66.5 days/cm). Mean lengthening was 9.3 cm (range, 5.8-12.1 cm). Based on the criteria recommended by Paley et al, 16 bone results were excellent, 6 good, and 1 fair. Fifteen functional results were excellent, 7 good, 1 fair, and 0 poor. This study shows that distraction osteogenesis with an external fixator is an effective treatment for massive postosteomyelitis bone defects and leg shortening.
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[Responsiveness evaluation of mental intervention services system in Wenchuan earthquake area].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 03-24-2011
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To understand the mental intervention service system responsiveness.
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Adeno-associated viral vector mediated and cardiac-specific delivery of CD151 gene in ischemic rat hearts.
J. Huazhong Univ. Sci. Technol. Med. Sci.
PUBLISHED: 02-19-2011
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Our previous studies demonstrated that CD151 gene promoted neovascularization in ischemic heart model. To improve the delivery efficacy and target specificity of CD151 gene to ischemic heart, we generated an adeno-associated virus (AAV) vector in which CD151 expression was controlled by the myosin light chain (MLC-2v) promoter to achieve the cardiac-specific expression of CD151 gene in ischemic myocardium and to limit unwanted CD151 expression in extracardiac organs. The function of this vector was examined in rat ischemic myocardium model. The protein expression of CD151 in the ischemic myocardium areas, liver and kidney was confirmed by using Western blot, while the microvessels within ischemic myocardium areas were detected by using immunohistochemistry. The results showed that MLC-2v significantly enhanced the expression of CD151 in ischemic myocardium, but attenuated its expression in other organs. The forced CD151 expression could increase the number of microvessels in the ischemic myocardium. This study demonstrates the AAV-mediated and MLC-2v regulated CD151 gene is highly expressed in the ischemic myocardium and cardiac-specific delivery that is more efficiently targets CD151 to the ischemia myocardium after myocardial infarction.
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Role of tetraspanin CD151-?3/?6 integrin complex: Implication in angiogenesis CD151-integrin complex in angiogenesis.
Int. J. Biochem. Cell Biol.
PUBLISHED: 01-10-2011
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Tetraspanin CD151 mainly associates with laminin-binding integrins and forms CD151-integrin complex. We previously reported that CD151 could be a potential target for angiogenesis, but the mechanisms involved are still unclear. This study investigated the role of CD151-integrin complex in angiogenesis and the signaling mechanisms involved. Here we showed that CD151 and CD151-AAA mutant were both well expressed at the protein level. CD151 gene transfer promoted angiogenesis and improved skin temperature of the lateral ischemic hindlimb, whereas CD151-AAA mutant abrogated the increase in capillary density and skin temperature. Further, CD151-AAA mutant failed to activate the FAK, ERK, PI3K/Akt/eNOS, and Rac1/Cdc42 signaling pathways. Moreover, CD151-AAA mutant was unavailable to promote bovine aortic endothelial cells (BAECs) proliferation and migration, in contrast to the effects of CD151. The results suggested that formation of CD151-integrin complex was likely to be a prerequisite for CD151-induced angiogenesis and signaling pathways.
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On the phylogeny of Mustelidae subfamilies: analysis of seventeen nuclear non-coding loci and mitochondrial complete genomes.
BMC Evol. Biol.
PUBLISHED: 01-02-2011
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Mustelidae, as the largest and most-diverse family of order Carnivora, comprises eight subfamilies. Phylogenetic relationships among these Mustelidae subfamilies remain argumentative subjects in recent years. One of the main reasons is that the mustelids represent a typical example of rapid evolutionary radiation and recent speciation event. Prior investigation has been concentrated on the application of different mitochondrial (mt) sequence and nuclear protein-coding data, herein we employ 17 nuclear non-coding loci (>15 kb), in conjunction with mt complete genome data (>16 kb), to clarify these enigmatic problems.
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[Relationship between the colonization of Group B Streptococci, Mycoplasma, and Chlamydia trachomatis infections and spontaneous abortion due to early embryonic death].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 11-06-2010
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To explore the relationship between the colonization of group B streptococci (GBS), mycoplasma,and Chlamydia trachomatis (CT) infections and spontaneous abortion due to early embryonic death.
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The involvement of occipital and inferior frontal cortex in the phonological learning of Chinese characters.
J Cogn Neurosci
PUBLISHED: 08-31-2010
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Neural changes related to the learning of the pronunciation of Chinese characters in English speakers were examined using fMRI. We examined the item-specific learning effects for trained characters and the generalization of phonetic knowledge to novel transfer characters that shared a phonetic radical (part of a character that gives a clue to the whole characters pronunciation) with trained characters. Behavioral results showed that shared phonetic information improved performance for transfer characters. Neuroimaging results for trained characters over learning found increased activation in the right lingual gyrus, and greater activation enhancement in the left inferior frontal gyrus (Brodmanns area 44) was correlated with higher accuracy improvement. Moreover, greater activation for transfer characters in these two regions at the late stage of training was correlated with better knowledge of the phonetic radical in a delayed recall test. The current study suggests that the right lingual gyrus and the left inferior frontal gyrus are crucial for the learning of Chinese characters and the generalization of that knowledge to novel characters. Left inferior frontal gyrus is likely involved in phonological segmentation, whereas right lingual gyrus may subserve processing visual-orthographic information.
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Detecting resting-state functional connectivity in the language system using functional near-infrared spectroscopy.
J Biomed Opt
PUBLISHED: 08-31-2010
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Functional connectivity has become one of the important approaches to understanding the functional organization of the human brain. Recently, functional near-infrared spectroscopy (fNIRS) was demonstrated as a feasible method to study resting-state functional connectivity (RSFC) in the sensory and motor systems. However, whether such fNIRS-based RSFC can be revealed in high-level and complex functional systems remains unknown. In the present study, the feasibility of such an approach is tested on the language system, of which the neural substrates have been well documented in the literature. After determination of a seed channel by a language localizer task, the correlation strength between the low frequency fluctuations of the fNIRS signal at the seed channel and those at all other channels is used to evaluate the language system RSFC. Our results show a significant RSFC between the left inferior frontal cortex and superior temporal cortex, components both associated with dominant language regions. Moreover, the RSFC map demonstrates left lateralization of the language system. In conclusion, the present study successfully utilized fNIRS-based RSFC to study a complex and high-level neural system, and provides further evidence for the validity of the fNIRS-based RSFC approach.
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A combined proteomics and metabolomics profiling of gastric cardia cancer reveals characteristic dysregulations in glucose metabolism.
Mol. Cell Proteomics
PUBLISHED: 08-10-2010
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Gastric cardia cancer (GCC), which occurs at the gastric-esophageal boundary, is one of the most malignant tumors. Despite its high mortality and morbidity, the molecular mechanism of initiation and progression of this disease is largely unknown. In this study, using proteomics and metabolomics approaches, we found that the level of several enzymes and their related metabolic intermediates involved in glucose metabolism were deregulated in GCC. Among these enzymes, two subunits controlling pyruvic acid efflux, lactate dehydrogenase A (LDHA) and pyruvate dehydrogenase B (PDHB), were further analyzed in vitro. Either down-regulation of LDH subunit LDHA or overexpression of PDH subunit PDHB could force pyruvic acid into the Krebs cycle rather than the glycolysis process in AGS gastric cancer cells, which inhibited cell growth and cell migration. Our results reflect an important glucose metabolic signature, especially the dysregulation of pyruvic acid efflux in the development of GCC. Forced transition from glycolysis to the Krebs cycle had an inhibitory effect on GCC progression, providing potential therapeutic targets for this disease.
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Bisaryldiketene derivatives: A new class of selective ligands for c-myc G-quadruplex DNA.
Bioorg. Med. Chem.
PUBLISHED: 08-07-2010
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A series of bisaryldiketene derivatives were designed and synthesized as a new class of specific G-quadruplex ligands. The ligand-quadruplex interactions were further evaluated by FRET, ITC, and PCR stop assay. In contrast to most of the G-quadruplex ligands reported so far, which comprise an extended aromatic ring, these compounds are neither polycyclic nor macrocyclic, but have a non-aromatic and relative flexible linker between two quinoline moieties enabling the conformation of compounds to be flexible. Our results showed that these bisaryldiketene derivatives could selectively recognize G-quadruplex DNA rather than binding to duplex DNA. Moreover, they showed promising discrimination between different G-quadruplex DNA. The primary binding affinity of ligand M2 for c-myc G-quadruplex DNA was over 200 times larger than that for telomere G-quadruplex DNA.
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[Role of abdominal obesity in asymptomatic intracranial arterial stenosis: a community-based study].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 07-15-2010
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To investigate the relationship between abdominal obesity and intracranial artery stenosis and the underlying mechanism.
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Curcumin protects intestinal mucosal barrier function of rat enteritis via activation of MKP-1 and attenuation of p38 and NF-?B activation.
PLoS ONE
PUBLISHED: 07-08-2010
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Intestinal mucosa barrier (IMB) dysfunction results in many notorious diseases for which there are currently few effective treatments. We studied curcumins protective effect on IMB and examined its mechanism by using methotrexate (MTX) induced rat enteritis model and lipopolysaccharide (LPS) treated cell death model.
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Over-expression of the AtGA2ox8 gene decreases the biomass accumulation and lignification in rapeseed (Brassica napus L.).
J Zhejiang Univ Sci B
PUBLISHED: 07-02-2010
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Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. The transgenic plants, identified by resistant selection, polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) analyses, and green fluorescence examination, showed growth retardation, flowering delay, and dwarf stature. The fresh weight and dry weight in transgenic lines were about 21% and 29% lower than those in wild type (WT), respectively, and the fresh to dry weight ratios were higher than that of WT. Quantitative measurements demonstrated that the lignin content in transgenic lines decreased by 10%-20%, and histochemical staining results also showed reduced lignification in transgenic lines. Quantitative real-time PCR analysis indicated that the transcript levels of lignin biosynthetic genes in transgenic lines were markedly decreased and were consistent with the reduced lignification. These results suggest that the reduced biomass accumulation and lignification in the AtGA2ox8 over-expression rapeseed might be due to altered lignin biosynthetic gene expression.
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The SH3 domain, but not the catalytic domain, is required for phospholipase C-gamma1 to mediate epidermal growth factor-induced mitogenesis.
Biochem. Biophys. Res. Commun.
PUBLISHED: 06-25-2010
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Phospholipase C-gamma1 (PLC-gamma1) is a multiple-domain protein and plays an important role in epidermal growth factor (EGF)-induced cell mitogenesis, but the underlying mechanism is unclear. We have previously demonstrated that PLC-gamma1 is required for EGF-induced mitogenesis of squamous cell carcinoma (SCC) cells, but the mitogenic function of PLC-gamma1 is independent of its lipase activity. Earlier studies suggest that the Src homology 3 (SH3) domain of PLC-gamma1 possesses mitogenic activity. In the present study, we sought to determine the role of the SH3 domain of PLC-gamma1 in EGF-induced SCC cell mitogenesis. We examined the effect of overexpression of PLC-gamma1, a catalytically active PLC-gamma1 mutant lacking the SH3 domain or a catalytically inactive PLC-gamma1 mutant lacking the X domain on EGF-induced SCC4 (tongue squamous cell carcinoma) cell mitogenesis. We found that overexpression of PLC-gamma1 enhanced EGF-induced SCC4 cell mitogenesis. This enhancement was abolished by deletion of the SH3 domain but not by deletion of the X catalytic domain. These data suggest that the SH3 domain, but not the catalytic domain, is required for PLC-gamma1 to mediate EGF-induced SCC4 cell mitogenesis.
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Insulin receptor substrate 1 regulates the cellular differentiation and the matrix metallopeptidase expression of preosteoblastic cells.
J. Endocrinol.
PUBLISHED: 06-04-2010
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Insulin receptor substrate 1 (IRS1) is an essential molecule for the intracellular signaling of IGF1 and insulin, which are potent anabolic regulators of bone metabolism. Osteoblastic IRS1 is essential for maintaining bone turnover; however, the mechanism underlying this regulation remains unclear. To clarify the role of IRS1 in bone metabolism, we employed RNA interference to inhibit IRS1 gene expression and observed the effects of silencing this gene on the proliferation and differentiation of and the expression of matrix metallopeptidase (MMP) and tumor necrosis factor receptor superfamily, member 11b (TNFRSF11B) in MC3T3-E1 cells. Our results showed that IRS1 short hairpin RNAs can effectively suppress the expression of IRS1, and inhibit the phosphorylation of AKT in IRS1 pathway; reduce the expression of MMP2, MMP3, MMP13, and MMP14, decrease the expression of TNFRSF11B and RANKL (also known as tumor necrosis factor (ligand) superfamily, member 11), however increase the RANKL/TNFRSF11B ratio; decrease cell survival, proliferation, and mineralization, and impair the differentiation of MC3T3-E1 cells. The downregulation of IRS1 had no effect on the expression of MMP1. Our findings suggest that IRS1 not only promotes bone formation and mineralization but also might play roles in bone resorption partly via the regulation of MMPs and RANKL/TNFRSF11B ratio, thus regulates the bone turnover.
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Synthesis and evaluation of graveoline and graveolinine derivatives with potent anti-angiogenesis activities.
Eur J Med Chem
PUBLISHED: 05-18-2010
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A series of graveoline and graveolinine derivatives were synthesized. The biological results showed that most of graveoline derivatives possessed higher cytotoxicity and better inhibitive effect against the adhesion and migration of human umbilical vein endothelial cell (HUVEC) than graveolinine derivatives. Among these compounds, 8d was the most potent agents that also showed significant anti-angiogenesis activities in chick embryo chorioallantoic membrane (CAM) assay.
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Mesenchymal stem cells cultured on tantalum used in early-stage avascular necrosis of the femoral head.
Med. Hypotheses
PUBLISHED: 05-12-2010
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Avascular necrosis of the femoral head (ANFH) is a common ailment characterized by necrosis of bone trabecular and bone marrow. Its mainly caused by the destruction of the blood supply in the femoral head and high rate of morbidity and disability is involved. Many methods have been tried including conservative treatment as drugs, electric stimulus, shock wave and electromagnetic field therapy and surgeries as core compression, rotary osteotomy and vascularized bone graft, but neither of them has definitive effect. Recently mesenchymal stem cells (MSCs) and tantalum robust have been widely used in ANFH. They have definite cure effects but advantages and disadvantages should not be neglected. In this paper, we hypothesize that mesenchymal stem cells cultured on tantalum can be used to prevent collapse of the articular surface in patients with ANFH. Unlike current treatment modalities, it would promote the regeneration of femoral head, provide structural support and allow for bony substitution over time.
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[Clinical characteristics and treatment assessments of severe enterovirus 71 infected children].
Zhonghua Er Ke Za Zhi
PUBLISHED: 05-06-2010
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To facilitate early recognition of ominous clinical manifestations, to understand pathophysiology and assess treatment effects in patients with severe enterovirus 71 (EV71) associated hand, foot and mouth disease (HFMD).
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[Research on the application of response variable transform method in high scattering medium with near-infrared spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 04-14-2010
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In the present paper, a new method named response variable transform (RVT) is proposed to reduce the effect of scattering in the NIR spectroscopy measurement. In the RVT method, the rates of the change in light intensity at different spatial positions with the concentration of the investigated composition are firstly sampled. Then, based on the sampled changing rates of light intensity, the absorption coefficients, which are used as new response variable, are calculated using the inverse adding-double (IAD) algorithm. Finally, through the partial least squares (PLS) regression, the calibration models for the investigated compositions are established using these new response variables. To validate the feasibility and effectiveness of the proposed method, the RVT method is applied to reduce the effect of scattering during the process of composition concentration measurement in milk. First, the relationships between the absorption coefficient and the concentrations of major compositions including fat and protein are analyzed. Then, with Monte Carlo simulations for different concentration levels of fat and protein, the influence of scattering on the relationship between the absorbency and the concentration is investigated and verified. Finally, for comparison, both the RVT method and the traditional preprocessing techniques are used to reduce the effect of scattering during the process of prediction model establishment. Experimental results showed that the prediction precision for fat and protein with the RVT method is increased by up to 33.9% and 46.5% than that with multiplicative signal correction (MSC) algorithm, and the prediction precision for fat and protein with the RVT method is increased by up to 55.9% and 33.7% than that with standard normal variate (SNV) algorithm, which also indicates that the RVT method is an effective method for eliminating the interference of scattering in the NIR spectroscopy measurement.
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[Cyclic strain promotes migration of human periodontal ligament cell via extracellular signal-regulated kinase (ERK) signaling pathway].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 04-07-2010
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To study the effect of cyclic strain on migration of human periodontal ligament cell(hPDLC) and underlying mechanism.
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[Soft tissue repairing and functional reconstruction in limb salvage operation on extremity bone malignant tumors].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 04-03-2010
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To investigate the effect and the significance of soft tissue repairing and functional reconstruction in limb salvage operation on extremity malignant bone tumors by individualized prosthesis replacement after malignant tumor resection with the help of Neo-adjuvant chemo-therapy.
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[Study on quantitative model for suspended sediment concentration in Taihu Lake].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 03-23-2010
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The complicated compositions of Case II waters result in the complex properties of spectral curves. The present paper analyzed the in situ measurements data of spectral curves, and further realized the relationships between the properties of spectral curves and suspended sediment concentration. The study found that the max peak of spectral curves was moving to the direction of shortwavelength as increasing suspended sediment concentration, namely the blue shift of wavelength; the area enclosed by spectral curve and coordinate axis in the range of sensitive bands had preferably linear relationship with the suspended sediment concentration (curve area model); the trapezoidal area model which was an approximation of curve area model could also excellently reflect those relationships, and be greatly suitable for multi-spectral satellite imagery retrieval such as LandSat/TM, MODIS and so on. The inversion results of trapezoidal area model for LandSat/TM imagery on October 27, 2003 in Taihu Lake showed that the suspended sediment concentration ranged from 30 to 80 mg x L(-1), the distribution pattern was higher in the west, south and central lake and lower in the east lake; compared with the in situ measurements in the regions, and the relative error of retrieval model was 6.035%.
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[Survivin antisense oligodeoxynucleotides inhibits the proliferation of hepatocellular carcinoma cells and enhances 5-FU sensitivity].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 02-18-2010
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To investigate the inhibitory effect of survivin antisense oligodeoxynuleotides (ASODN) mediated by polyethylenimine (PEI) on hepatocelluar carcinoma SMMC-7721 cell proliferation and its effect on chemosensitivity to 5-FU in tumor-bearing mice.
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[Survivin antisense oligodeoxynucleotides inhibits the proliferation of hepatocellular carcinoma cells and enhances the cell chemosensitivity to 5-Fu].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 11-20-2009
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To investigate the inhibitory effect of survivin antisense oligodeoxynucleotides (ASODN) mediated by polyethylenimine(PEI) on the proliferation of hepatocellular carcinoma SMMC-7721 cells, and assess its detect on the chemosensitivity of the cells to 5-FU.
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[Application of the Childrens Impact of Event Scale (Chinese Version) on a rapid assessment of posttraumatic stress disorder among children from the Wenchuan earthquake area].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 10-19-2009
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To explore the reliability and validity of the Childrens Impact of Event Scale (Chinese version, CRIES-13) and to determine the value and the optimal cutoff point of the score of CRIES-13 in screening posttraumatic stress disorder (PTSD), so as to provide evidence for PTSD prevention and identify children at risk in Wenchuan earthquake areas.
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[Intra-individual allele heterozygotes in phylogenetic analysis].
Yi Chuan
PUBLISHED: 10-13-2009
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The study of evolutionary relationships among organisms is vital in evolutionary biology. To reconstruct a reliable species phylogeny, one of the most important issues is to choose proper molecular markers and take full advantage of phylogenetic information contained in these markers. Intra-individual allele heterozygotes (IIAHs) have been commonly detected in intron phylogenetic studies. How to incorporate IIAHs into phylogenetic framework has been a focus in current studies. In this review, the conception, isolation, and analytic methods of IIAHs in phylogeny were summarized.
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Intrathecal injection of GluR6 antisense oligodeoxynucleotides alleviates acute inflammatory pain of rectum in rats.
Neurosci Bull
PUBLISHED: 09-29-2009
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To investigate whether the kainate (KA) receptor subunit GluR6 is involved in the acute inflammatory pain.
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[The crown inclination of the teeth and its clinical value].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 09-21-2009
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The crown inclination, representing an angle formed by the clinical crown axis and a line which bears 90 degrees to the occlusal plane, reflects the labiolingual or buccolingual inclination of the long axis of the crown. The crown inclination is related to the races. And it is compensated by different vertical or sagittal jaw relationships. It plays an important role in fine occlusal relationship and achieving functional, artistic and stable treatment goal. The torque design of the straight wire bracket is based on the normal crown inclination. There is much discrepancy in the torque design of the different straight wire bracket because of the different race and philosophy. This review gave a brief introduction of the influential factors, the clinical meaning of the crown inclination and the torque design of the different bracket system.
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Use of fNIRS to assess resting state functional connectivity.
J. Neurosci. Methods
PUBLISHED: 08-01-2009
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Recently, resting state functional connectivity (RSFC) studies based on fMRI and EEG/MEG have provided valuable insight into the intrinsic functional architecture of the human brain. However, whether functional near infrared spectroscopy (fNIRS), a suitable imaging method for infant and patient populations, can be used to examine RSFC remains elusive. Using an ETG-4000 Optical Topography System, the present study measured 29 adult subjects (14 females) over the sensorimotor and auditory cortexes during a resting session and a motor-localizer task session. The RSFC maps were computed by seed-based correlation analysis and data-driven cluster analysis. The results from both analyses showed robust RSFC maps, which were not only consistent with the localizer task-related activation results, but also those of previous fMRI findings. Moreover, the strong consistency between the seed-based correlation analysis and the data-driven cluster analysis further validated the use of fNIRS to assess RSFC. The potential influence of a specific low-frequency filtering range (0.04-0.15 Hz and 0.01-0.08 Hz) and three fNIRS parameters (oxy-Hb, deoxy-Hb, and total-Hb) on RSFC results were also examined.
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[Research on the robustness improvement of calibration model for measuring the contents of components in milk by multidimensional calibration in near-infrared spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 07-25-2009
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A new hybrid algorithm (NOSC-NPLS), the combination of multi-way orthogonal signal correction (N-OSC) algorithm and multi-way partial least squares (N-PLS) algorithm, is proposed. In NOSC-NPLS algorithm, the 3-D spectral matrix was firstly constructed, which is composed of the temperature information and the NIR spectrum. Secondly, the N-OSC algorithm was used as a pretreatment algorithm to remove the interference information irrelevant to analyte in 3-D spectral matrix. Finally, the N-PLS algorithm was applied to develop the calibration model based on the pretreated 3-D transmission spectral matrix and content matrix of major components in milk. In order to evaluate the performances of conventional algorithms and multidimensional calibration algorithms on suppressing the effects of temperature variation, a batch of milk samples at temperature of 25, 30, 35 and 40 degrees C were measured in the wavelength range from 1,100 to 1,700 nm and the experimental results were investigated. It was found that the conventional algorithms, which could not suppress the effects caused by temperature variation, failed to obtain satisfactory results. However, compared with these algorithms, the experimental results showed that the NOSC-NPLS algorithm can effectively eliminate the effects of temperature variation and also can help achieve the analytic models with better prediction ability and robustness.
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Multipathogen infections in hospitalized children with acute respiratory infections.
Virol. J.
PUBLISHED: 07-15-2009
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To explore the epidemiologic and clinical features of, and interactions among, multipathogen infections in hospitalized children with acute respiratory tract infection (ARTI). A prospective study of children admitted with ARTI was conducted. Peripheral blood samples were analyzed by indirect immunofluorescence to detect respiratory agents including respiratory syncytial virus; adenovirus; influenza virus (Flu) types A and B; parainfluenza virus (PIV) types 1, 2, and 3; chlamydia pneumonia; and mycoplasma pneumonia. A medical history of each child was taken.
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[A study on apoptosis and apoptotic mechanisms of HL-7702 cell line induced by methylmercury].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 06-19-2009
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To study the apoptotic effect and mechanisms of methylmercury (MeHg) on HL-7702 cell line in vitro.
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Magnetically driven solid sample preparation for centrifugal microfluidic devices.
Anal. Chem.
PUBLISHED: 05-09-2009
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A prototype for solid sample preparation on centrifugal microfluidic devices has been designed and characterized. The system uses NdFeB magnets in both the centrifugal device and a fixed base. As the centrifugal device rotates, the magnets move and spin in their chambers creating a pulverizing mechanical motion. This technique was successfully applied to the dissolution of potassium ferricyanide (K(3)[Fe(CN)(6)]), a hard colored crystal. A 0.10 g sample was completely dissolved in 3 s in 1.0 mL of water while rotating at 1000 rpm. This is a 300-fold improvement over static dissolution.
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[Expressing borna disease virus phosphoprotein in PC-12 cell and its influence on the cell proliferation].
Wei Sheng Wu Xue Bao
PUBLISHED: 04-25-2009
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To establish stable expressing system of Borna disease virus (BDV) phosphoprotein in PC-12 cells, and then study its influence on cell proliferation of PC-12 cells.
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Astrocyte elevated gene-1 regulates osteosarcoma cell invasion and chemoresistance via endothelin-1/endothelin A receptor signaling.
Oncol Lett
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Astrocyte elevated gene-1 (AEG-1) and endothelin-1 (ET-1)/endothelin A receptor (ETAR) signaling have been demonstrated to be important in osteosarcoma (OS) progression. In the present study, we explored the interaction between AEG-1 and ET-1/ETAR signaling in OS cells, and investigated the mechanism(s) through which the functional interaction may impact OS cell invasion and chemoresistance. Overexpression and knockdown of AEG-1 were performed in Saos-2 and MG-63 OS cells, respectively. Overexpression of AEG-1 in Saos-2 cells significantly increased ET-1 expression (at both the mRNA and protein levels), cell invasion, MMP-2 expression and cell survival against cisplatin. These effects were eradicated using a selective phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002, or a selective ETAR inhibitor, BQ123. Knockdown of AEG-1 in MG-63 cells significantly decreased ET-1 expression (at both the mRNA and protein levels), cell invasion, MMP-2 expression and cell survival against cisplatin. Exogenous ET-1 restored cell invasion and MMP-2 expression levels in MG-63 cells, in which AEG-1 had been knocked down, in the presence of LY294002, but not in the presence of BQ123. However, exogenous ET-1 only partially rescued cell survival against cisplatin-induced apoptosis in the presence of LY294002, in cells in which AEG-1 had been knocked down. In conclusion, we have demonstrated that AEG-1 regulates ET-1 expression at the transcriptional level in a PI3K-dependent manner in OS cells. Downstream of PI3K, ET-1/ETAR signaling primarily mediates the promoting effect of AEG-1 on OS cell invasion, likely through the upregulation of MMP-2 expression, thus, ET-1/ETAR signaling partially, but significantly, mediates the AEG-1-induced chemoresistance in OS cells. To the best of our knowledge, this study has provided the first evidence of a functional association between AEG-1 and ET-1/ETAR signaling in OS cells, which adds novel insights into the molecular mechanism of OS metastasis and chemoresistance.
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The tetraspanin CD151-ARSA mutant inhibits angiogenesis via the YRSL sequence.
Mol Med Rep
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Previous studies have shown that the tetraspanin CD151 is essential for pathological or physiological angiogenesis. However, the cellular signaling mechanism and the role of the CD151 YRSL sorting motif in in vitro vasculogenesis remains unknown. In this study, the results showed that both CD151 and CD151-ARSA gene delivery were capable of increasing the expression of CD151 at the protein level in human umbilical vein endothelial cells (HUVECs). Moreover, there was no significant difference in CD151 protein expression between the CD151 group and the CD151-ARSA group. Overexpression of CD151 promoted HUVEC cell proliferation, migration and capillary network formation in vitro. However, in the CD151-ARSA group, the abilities of cell proliferation, migration and capillary network formation were all decreased, compared with the CD151 group. Furthermore, the activation of PI3K, Akt and ERK signaling pathways was attenuated in the CD151-ARSA mutant group compared with the CD151 group. This study suggests that the YRSL motif of CD151 plays a key role in CD151-induced angiogenesis. Our observations provide insights into a new mechanism of CD151 regulating angiogenesis via vesicle trafficking.
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Nonstructural protein-1 of respiratory syncytial virus regulates HOX gene expression through interacting with histone.
Mol. Biol. Rep.
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Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, constitutes an important risk factor for the development of subsequent asthma. In searching for its mechanism, the present study was designed to screen the interacting proteins of two important nonstructural (NS) proteins in human BECs. The subcellular localization and the effects of NS on HOX gene expression were also examined. The results showed that NS1 was distributed throughout the nucleus and cytoplasm, while NS2 was mainly distributed in cytoplasm of BECs. NS1 interacted specifically with host histone H2BD, inducing histone ubiquitination and subsequent HOX gene expression. In conclusion, the results of the present study indicated that RSV NS-1 induces HOX gene expression, through histone ubiquitination in a BEC cell line, which may provide a novel conception for understanding the relationship between severe RSV bronchiolitis during early life and the development of subsequent asthma.
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[hMLH1 gene promoter methylation in joint cartilage in patients with osteoarthritis].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
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To investigate the role of Human MutL homologue 1 (hMLH1) gene promoter methylation in the occurrence and development of osteoarthritis (OA).
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Association of ACE I/D gene polymorphism with vesicoureteral reflux susceptibility in children: a meta-analysis.
J Renin Angiotensin Aldosterone Syst
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Many studies have been conducted to investigate the association between angiotensin-converting enzyme (ACE) insertion (I)/deletion (D) gene polymorphism and vesicoureteral reflux (VUR) susceptibility. However, the results from those studies are still conflicting. We performed a meta-analysis of studies relating the ACE I/D gene polymorphism to the risk of VUR.
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Relationship between angiotensin-converting enzyme insertion/deletion gene polymorphism and systemic lupus erythematosus/lupus nephritis: a systematic review and metaanalysis.
J. Rheumatol.
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Results from studies of the association between angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism and systemic lupus erythematosus (SLE)/lupus nephritis (LN) are controversial. We performed this metaanalysis to evaluate the relationship between ACE I/D gene polymorphism and SLE/LN and to explore whether the ACE D allele or DD genotype could become a predictive marker for risk of SLE/LN.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.