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Find video protocols related to scientific articles indexed in Pubmed.
Activity of Pramlintide, Rat and Human Amylin but not A?1-42 at Human Amylin Receptors.
Endocrinology
PUBLISHED: 12-20-2013
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Amylin is a neuroendocrine hormone involved in glucose regulation. An amylin analog, pramlintide, is used to treat insulin-requiring diabetes. Its anorexigenic actions give it potential as an obesity treatment. There are 3 amylin receptors (AMY1, AMY2, AMY3), comprising the calcitonin receptor and receptor activity-modifying proteins 1, 2, and 3, respectively. The pharmacology of pramlintide at each subtype has not been determined whereas the unrelated peptide ?-amyloid 1-42 (A?1-42) has recently been proposed to be a specific agonist of the AMY3 receptor. We investigated the actions of A?1-42 and pramlintide, compared with human and rat amylin at the calcitonin receptor, AMY1, AMY2, and AMY3 receptors, measuring the cAMP response in human embryonic kidney 293S and Cos 7 cells. Pramlintide activated all receptors with a slight preference for AMY1. No cAMP response was detected with A?1-42 at any receptor, suggesting that it may not be a genuine agonist of AMY receptors.
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Optimization of periprosthetic culture for diagnosis of Propionibacterium acnes prosthetic joint infection.
J. Clin. Microbiol.
PUBLISHED: 05-04-2011
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Propionibacterium acnes is increasingly recognized as an important agent of prosthetic joint infection (PJI). However, the optimum culture conditions for recovery of this organism from PJI specimens have not been determined. By applying a prolonged 28-day culture incubation to all periprosthetic specimens received for bacterial culture from 198 revision arthroplasty procedures, we retrospectively determined that a 13-day culture incubation period is necessary for the recovery of P. acnes from patients with PJI. Incubation beyond this period was associated with increasing recovery of nondiagnostic isolates: 21.7% of P. acnes isolates believed to be clinically unimportant were recovered after 13 days of incubation. Importantly, a diagnosis of P. acnes PJI would have been missed in 29.4% of patients had extended culture incubation been applied only to anaerobic culture media. Although specimens from P. acnes PJIs were more commonly associated with the presence of ? 2 culture media positive for growth, acute inflammation (? 5 neutrophils/high-power field) was observed in only 40% of patients with PJIs that had more than one specimen submitted for bacterial culture. These results support the need for a minimum culture incubation period of 13 days to be applied to both aerobic and anaerobic culture media for all periprosthetic specimens. Optimal recovery of infecting organisms from PJI specimens will be an important component in generating a universal definition for PJI due to indolent agents of infection, such as P. acnes.
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Radiolucencies surrounding a smooth-stemmed radial head component may not correlate with forearm pain or poor elbow function.
J Shoulder Elbow Surg
PUBLISHED: 06-06-2009
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Radiolucencies surrounding the stems of smooth-stemmed metal radial head prostheses placed for comminuted radial head fractures are associated with proximal radial forearm pain.
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Healed cuff repairs impart normal shoulder scores in those 65 years of age and older.
Clin. Orthop. Relat. Res.
PUBLISHED: 01-30-2009
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It is unclear whether repaired rotator cuffs heal in older patients and whether the function in those shoulders compares with those of similarly aged patients with untreated tears.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.