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Find video protocols related to scientific articles indexed in Pubmed.
Development of a DNA microarray method for detection and identification of all 15 distinct O-antigen forms of Legionella pneumophila.
Appl. Environ. Microbiol.
PUBLISHED: 08-23-2013
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Legionella is ubiquitous in many environments. At least 50 species and 70 serogroups of the Gram-negative bacterium have been identified. Of the 50 species, 20 are pathogenic, and Legionella pneumophila is responsible for the great majority (approximately 90%) of the Legionnaires disease cases that occur. Furthermore, of the 15 L. pneumophila serogroups identified, O1 alone causes more than 84% of the Legionnaires disease cases that occur worldwide. Rapid and reliable assays for the detection and identification of L. pneumophila in water, environmental, and clinical samples are in great demand. L. pneumophila bacteria are traditionally identified by their O antigens by immunological methods. We have recently developed an O serogroup-specific DNA microarray for the detection of all 15 distinct O-antigen forms of L. pneumophila, including serogroups O1 to O15. A total of 35 strains were used to verify the specificity of the microarray, including 15 L. pneumophila O-antigen standard reference strains and seven L. pneumophila clinical isolates as target strains, seven reference strains of other non-pneumophila Legionella species as closely related strains, and six non-Legionella bacterial species as nonrelated strains. The detection sensitivity was 1 ng of genomic DNA or 0.4 CFU/ml in water samples with filter enrichment and plate culturing. This study demonstrated that the microarray allows specific, sensitive, and reproducible detection of L. pneumophila serogroups. To the best of our knowledge, this is the first report of a microarray serotyping method for all 15 distinct O-antigen forms of L. pneumophila.
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Use of a DNA microarray for detection and identification of bacterial pathogens associated with fishery products.
Appl. Environ. Microbiol.
PUBLISHED: 09-30-2011
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We established a microarray for the simultaneous detection and identification of diverse putative pathogens often associated with fishery products by targeting specific genes of Listeria monocytogenes, Salmonella, Shigella, Staphylococcus aureus, Streptococcus pyogenes, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, and Yersinia enterocolitica and the 16S-23S rRNA gene internal transcribed spacer (ITS) region of Proteus mirabilis and Proteus vulgaris. The microarray contained 26 specific probes and was tested against a total of 123 target bacterial strains that included 55 representative strains, 68 clinical isolates, and 45 strains of other bacterial species that belonged to 8 genera and 34 species, and it was shown to be specific and reproducible. A detection sensitivity of 10 ng DNA or 10 CFU/ml for pure cultures of each target organism demonstrated that the assay was highly sensitive and reproducible. Mock and real fishery product samples were tested by the microarray, and the accuracy was 100%. The DNA microarray method described in this communication is specific, sensitive, and reliable and has several advantages over traditional methods of bacterial culture and antiserum agglutination assays.
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[Preventive and therapeutic effects of quercetin on hyperuricemia and renal injury in rats].
Wei Sheng Yan Jiu
PUBLISHED: 05-13-2011
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To study the preventive and therapeutic effects of Quercetin (Que) on hyperuricemia and renal failure in rats.
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Chromatin immunoprecipitation and association study revealed a possible role of Runt-related transcription factor 3 in the ulcerative colitis of Chinese population.
Clin. Immunol.
PUBLISHED: 01-11-2010
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RUNX3 was recently found to be associated with ulcerative colitis. In this study, downstream target genes of RUNX3 were identified by chromatin immunoprecipitation and promoter sequence microarray chips. Polymorphisms of RUNX3 and its 2 putative downstream (OCTN1 and PPAR-gamma) target genes were genotyped by PCR-SSP and sequencing in 144 Chinese UC patients and 151 healthy controls. Expression of RUNX3 in colonic mucosa of UC patients was detected by immunohistochemical staining. Twelve genes involved in IBD were identified as the downstream target genes of RUNX3. The RUNX3 rs2236851 CT genotype was associated significantly with UC susceptibility and risk of early onset in Chinese population. No association of OCTN1 and PPAR-gamma with UC susceptibility or subphenotypes was identified. RUNX3 expression was significantly increased in UC mucosa. Therefore, RUNX3 might be involved in UC pathogenesis by regulating the expression of genes related with immune response.
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Effects of elevated O? concentration on winter wheat and rice yields in the Yangtze River Delta, China.
Environ. Pollut.
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The effects of a continuing rise of ambient ozone on crop yield will seriously threaten food security in China. In the Yangtze River Delta, a rapidly developing and seriously air polluted region in China, innovative open-top chambers have been established to fumigate winter wheat and rice in situ with elevated O(3). Five years of study have shown that the yields of wheat and rice decreased with increasing O(3) concentration. There were significant relationships between the relative yield and AOT40 (accumulated hourly O(3) concentration over 40 ppb) for both winter wheat and rice. Winter wheat was more sensitive to O(3) than rice. O(3)-induced yield declines were attributed primarily to 1000-grain weight and harvest index for winter wheat, and attributed primarily to grain number per panicle and harvest index for rice. Control of ambient O(3) pollution and breeding of O(3) tolerant crops are urgent to guarantee food security in China.
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RUNX3 mediates suppression of tumor growth and metastasis of human CCRCC by regulating cyclin related proteins and TIMP-1.
PLoS ONE
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Here we presented that the expression of RUNX3 was significantly decreased in 75 cases of clear cell renal cell carcinoma (CCRCC) tissues (p<0.05). Enforced RUNX3 expression mediated 786-O cells to exhibit inhibition of growth, G1 cell-cycle arrest and metastasis in vitro, and to lost tumorigenicity in nude mouse model in vivo. RUNX3-induced growth suppression was found partially to regulate various proteins, including inhibition of cyclinD1, cyclinE, cdk2, cdk4 and p-Rb, but increase of p27(Kip1), Rb and TIMP-1. Therefore, RUNX3 had the function of inhibiting the proliferative and metastatic abilities of CCRCC cells by regulating cyclins and TIMP1.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.