Multifunctional nanoparticles (NPs) have been designed for a variety of cell imaging and therapeutic applications, and the study of their cellular interactions is crucial to the development of more efficient biomedical applications. Among current nanomaterials, concave core-shell NPs with complex angled geometries are attractive owing to their unique shape-dependent optical and physical properties as well as different tendency for cell interaction. In this study, we investigated the morphology effect of spiky gold-coated iron oxide supraparticles (Fe3O4@Au SPs) on cytotoxicity and global gene expression in sarcoma 180 cells. Cells treated for 7 days with spiky supraparticles (SPs) at concentrations up to 50 ?g/mL showed >90% viability, indicating that these NPs were nontoxic. To shed light on the differences in cytotoxicity, we monitored the expression of 33?315 genes using microarray analysis of SP-treated cells. The 171 up-regulated genes and 181 down-regulated genes in spiky SP-treated cells included Il1b, Spp1, Il18, Rbp4, and Il11ra1, where these genes are mainly involved in cell proliferation, differentiation, and apoptosis. These results suggested that the spiky Fe3O4@Au SPs can induce noncytotoxicity and gene expression in tumor cells, which may be a promising cornerstone on which to base related research such as cyto-/genotoxicology of nanomaterials or the design of nanoscale drug carriers.
JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.
How does it work?
We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.
Video X seems to be unrelated to Abstract Y...
In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.