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Find video protocols related to scientific articles indexed in Pubmed.
Application of a motion capture data glove for hand and wrist ergonomic analysis during laparoscopy.
Minim Invasive Ther Allied Technol
PUBLISHED: 06-09-2014
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Abstract Objectives: This study aims to analyze the surgeons' hand spatial configuration during the use of two different instrument handles for laparoscopy, by obtaining information from the data glove CyberGlove®, and establishing existing risk levels for wrist disorders.
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Ergonomics problems due to the use and design of dissector and needle holder: a survey in minimally invasive surgery.
Surg Laparosc Endosc Percutan Tech
PUBLISHED: 04-09-2014
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This study analyzes the problems and consequences associated with prolonged use of laparoscopic instruments (dissector and needle holder) and equipments.
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Development and initial assessment of a training program for laparoscopic radical prostatectomy. First module: the urethrovesical anastomosis.
J. Endourol.
PUBLISHED: 04-03-2014
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We present our experience in the design and development of a training program in laparoscopic radical prostatectomy (LRP), and the validation of the first steps of the program by objective measurement of the attendants' skills improvement and subjective evaluation of its contents.
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Comparison of single access devices during cut and suturing tasks on simulator.
J. Surg. Res.
PUBLISHED: 02-26-2014
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Published comparisons of the different available laparoendoscopic single-site surgery (LESS) devices focused on its economic cost and technical aspects. With this study, we aimed to objectively compare the use of three different LESS access devices in controlled experimental tasks.
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Deregulated expression of selected histone methylases and demethylases in prostate carcinoma.
Endocr. Relat. Cancer
PUBLISHED: 11-09-2013
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Prostate cancer (PCa), a leading cause of cancer-related morbidity and mortality, arises through the acquisition of genetic and epigenetic alterations. Deregulation of histone methyltransferases (HMTs) or demethylases (HDMs) has been associated with PCa development and progression. However, the precise influence of altered HMTs or HDMs expression and respective histone marks in PCa onset and progression remains largely unknown. To clarify the role of HMTs and HDMs in prostate carcinogenesis, expression levels of 37 HMTs and 20 HDMs were assessed in normal prostate and PCa tissue samples by RT-qPCR. SMYD3, SUV39H2, PRMT6, KDM5A, and KDM6A were upregulated, whereas KMT2A-E (MLL1-5) and KDM4B were downregulated in PCa, compared with normal prostate tissues. Remarkably, PRMT6 was the histone modifier that best discriminated normal from tumorous tissue samples. Interestingly, EZH2 and SMYD3 expression levels significantly correlated with less differentiated and more aggressive tumors. Remarkably, SMYD3 expression levels were of independent prognostic value for the prediction of disease-specific survival of PCa patients with clinically localized disease submitted to radical prostatectomy. We concluded that expression profiling of HMTs and HDMs, especially SMYD3, might be of clinical usefulness for the assessment of PCa patients and assist in pre-therapeutic decision-making.
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Performance analysis on physical simulator of four different instrument setups in laparo-endoscopic single-site (LESS) surgery.
Surg Endosc
PUBLISHED: 08-14-2013
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Over the past decades, minimally invasive surgery has undergone continuous development due to the demand for scarless results, with laparo-endoscopic single-site (LESS) surgery constituting one of todays most favored alternatives. In this study, we aim to assess the relative technical difficulty and performance benefits of dynamic articulating and pre-bent instruments, either combined with conventional laparoscopic tools or not, during the completion of two basic tasks hands-on simulator.
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Ergonomic analysis of muscle activity in the forearm and back muscles during laparoscopic surgery: influence of previous experience and performed task.
Surg Laparosc Endosc Percutan Tech
PUBLISHED: 04-13-2013
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The first aim of this study is to analyze the muscle activity in back and forearm muscles in surgeons during laparoscopic dissection and suturing maneuvers. The second aim is to determine the influence of the surgeons previous experience in laparoscopic surgery.
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[Training and research in urologic laparoscopic surgery. Design of training programs and experimental models of renal tumor].
Arch. Esp. Urol.
PUBLISHED: 02-15-2013
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We present our experience with surgical training programs development for basic and advanced laparoscopic urological surgery. Both training programs consist of 21 and 28 hours respectively. Basic surgical programs start with general knowledge of ergonomics and instrumentation, there after, attendants acquire basic skills on physical simulator. Posteriorly, techniques on animal model are undertaken, always assisted by an expert. Advanced activities start with surgical tasks on physical simulator. Posteriorly, reconstructive urological surgical techniques are undertaken on animal model, focused on partial nephrectomy, and always assisted by an expert tutor. We present our results on exophytic renal tumour model creation based chromatic Alginate.
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[Ergonomics in laparoscopic surgery and its importance in surgical training].
Cir Esp
PUBLISHED: 03-03-2011
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Despite the many advantages that laparoscopic surgery has for patients, it involves a series of risks for the surgeon. These are related to the reduced freedom of movement and forced postures which lead to greater muscle fatigue than with conventional surgery. In laparoscopic surgery there are few references on the introduction of training programs in ergonomics, despite the numerous advantages demonstrated in other disciplines. The application of ergonomic criteria in the surgical field could have great benefits, both for surgeons and patients. In this work we attempt to review the existing literature and our experience to provide the surgeon with some ergonomic guidelines for body stance and positioning of equipment. We also present a training model based on ergonomics which we have introduced into the training activities carried out in our Centre.
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Lessons from the genomes of extremely acidophilic bacteria and archaea with special emphasis on bioleaching microorganisms.
Appl. Microbiol. Biotechnol.
PUBLISHED: 06-30-2010
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This mini-review describes the current status of recent genome sequencing projects of extremely acidophilic microorganisms and highlights the most current scientific advances emerging from their analysis. There are now at least 56 draft or completely sequenced genomes of acidophiles including 30 bacteria and 26 archaea. There are also complete sequences for 38 plasmids, 29 viruses, and additional DNA sequence information of acidic environments is available from eight metagenomic projects. A special focus is provided on the genomics of acidophiles from industrial bioleaching operations. It is shown how this initial information provides a rich intellectual resource for microbiologists that has potential to open innovative and efficient research avenues. Examples presented illustrate the use of genomic information to construct preliminary models of metabolism of individual microorganisms. Most importantly, access to multiple genomes allows the prediction of metabolic and genetic interactions between members of the bioleaching microbial community (ecophysiology) and the investigation of major evolutionary trends that shape genome architecture and evolution. Despite these promising beginnings, a major conclusion is that the genome projects help focus attention on the tremendous effort still required to understand the biological principles that support life in extremely acidic environments, including those that might allow engineers to take appropriate action designed to improve the efficiency and rate of bioleaching and to protect the environment.
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SALMATcor: microagglutination for Salmonella flagella serotyping.
Foodborne Pathog. Dis.
PUBLISHED: 04-07-2010
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Salmonella is a complex bacterial group with more than 2400 serovars widely distributed in nature; they are considered zoonotic because they can infect a variety of animals and be transmitted to humans. Usually, they cause alimentary acquired diseases such as gastroenteritis, typhoid fever, and others that can lead to severe complications and death. Serotyping is useful to differentiate among Salmonella, because it shows an important correlation with their clinical and epidemiological patterns; consequently, it is of high value for public health, animal health, agriculture, and industry. To characterize all known Kauffmann-White Salmonella serovars, over 250 antisera are required. Due to this and to high prices antisera, many laboratories worldwide have limitations in establishing Salmonella surveillance. Therefore, we developed and validated a Salmonella flagella microagglutination test (SALMATcor) that significantly reduces laboratory requirements of antisera. SALMATcor is based on scaling down, by fivefold, the antigen:antiserum volumes actually required for the reference method: flagella standard tube agglutination technique (STAT). Antigen preparation, temperatures, and incubation periods remained as established for STAT. The SALMATcor was validated according to ISO/DIS 16140:1999 protocol, which included 1187 comparisons of flagella determinations conducted by SALMATcor and STAT, on 141 Salmonella isolates of 12 common serotypes and the use of antiserum recommended for STAT. SALMATcor concordance was excellent (Cohens kappa index 0.9982), obtaining relative accuracy >99.9% and relative specificity >99.9%. Additionally, SALMATcor has been used by CNRB-INCIENSA since 2004 to respond to all 40 Salmonella proficiency testing strains, provided by World Health Organization-Global Salmonella Surveillance Network, obtaining 100% concordance on serovar identification. On the basis of the results achieved with SALMATcor and considering that it also significantly reduces antiserum expenses, hand labor, glassware, and bench top and water bath space requirements (microtiter plates and micropipette tips are the only additional supplies), we envision that SALMATcor will contribute to establish a sustainable Salmonella serovar surveillance worldwide.
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[Training design and improvement of technical skills in the transvaginal cholecystectomy (NOTES)].
Cir Esp
PUBLISHED: 02-17-2009
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The current surgical scenario of the surgery through natural orifices or requires acquiring new technical skills by the surgeon. We introduce the initial experience of the Minimally Invasive Surgery Centre Jesús Usón (MISCJU) in the design and setting-up of a surgical training programme using the the natural orifices approach for the acquisition of surgical skills and abilities, based on the preliminary trials in simulators and a pig model.
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Effect of germinated soy protein on the growth of HeLa cervical cancer cells in female athymic mice.
Nutr Cancer
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Previous studies showed that germination could improve the antiproliferative effect of soy protein on cervical cancer cells and that a peptide fraction (MAPF) from germinated soybeans decreases the expression of PTTG1 and TOP2A (2 genes considered as therapeutic targets) causing apoptosis of cancer cells. The aim of this work was to study the effect of feeding germinated soybean protein diets on the tumor growth in nude mice inoculated with cervical cancer cells and identify the bioactive component. Mice were randomly assigned to 1 of the 6 dietary groups based in AIN-93G formulation with 6 protein sources: casein, ungerminated soy protein (SP), and SP from 2 and 6 days of germination, with and without ethanol-soluble phytochemicals (ESPC). Compared with casein-fed controls, the tumor volumes after 5 wk were reduced by 44.6% by ungerminated SP, 98.9% by 2-day-germinated SP, 97.7% by 2-day-germinated SP without ESPC, 94.7% by 6-day-germinated SP, and 92.7% by 6-day-germinated SP without ESPC (P < 0.05). Liquid chromatography coupled with tandem mass spectrometry analysis of MAPF showed that the bioactive peptide might be the leginsulin, a peptide involved in signal transduction of soybean cells. Germination is a simple procedure that could help to increase the anticancer activity of soy protein probably through generation of biologically active peptides.
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Thermal comfort and personal protective equipment (PPE).
Work
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Personal Protective Equipment (PPE) is considered the last preferred solution to an Occupational Safety Health (OSH) system. Brazilian OSH legislation assumes and requires PPE usage to neutralize the possibility of pesticide contamination. This study examines the inadequacy of the PPE used in a tomato crop, particularly from the standpoint of thermal comfort. This study made observations regarding heart rate and body temperature in a controlled environment using a treadmill (with and without PPE) of 12 volunteers; and during the process of rural labor (at rest and in normal work routine) of 2 actual rural workers. Comparing the results of the treadmill test (with and without PPE) which tried to reproduce the same level of effort and environmental conditions of the actual rural work, this study demonstrated that PPE makes thermoregulation harder and tympanic temperature reaches higher values with the same level of effort. Moreover, body temperature increases quickly: with PPE it took 15 minutes to raise 1°C in body temperature, compared to 40 minutes without PPE. The results of this study indicated that the use inadequate PPE might pose thermoregulation risk to rural workers.
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The start up as a phase of architectural design process.
Work
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Alterations made in the architectural design can be considered as a continuous process, from its conception to the moment a built environment is already in use. This article focuses on the "moving phase", which is the initial moment of the environment occupation and the start-up of services. It aims to show that the continuity of ergonomics interventions during the "moving phase" or start up may reveal the built environment inadequacies; clearly showing needs not met by the design and allowing making instant decisions to solve non-foreseen problems. The results have revealed some lessons experienced by users during a critical stage not usually included in the design process.
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Intermediary objects in the workspace design process: means of experience transfer in the offshore sector.
Work
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The aim of this paper is to discuss the use of intermediary objects in the workspace design process of offshore accommodations module. The integration of ergonomics in the design process can lead to better work conditions, more effectiveness in the work process and less health and safety issues. Moreover, it is more efficient in terms of cost if ergonomics is considered from the initial phases of the project, as the potential costs of the redesign, the possible losses and the down-time in the operation of the platform would be more increased. The goal, then, is to discuss the integration of ergonomics and users involvement in the design process of accommodations modules, focusing on the transfer of information from reference situations by the use of intermediary objects during the process. In this paper we will present two tools developed to be used as intermediary object(s) aiming at transferring the experience from the use to the design in the specific field of offshore accommodations module.
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Ergonomics in projects of oil platforms in a change context.
Work
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The context of oil platform design is changing in order to increase competitiveness and be prepared for difficult operations, mainly in fields more distant from the coast, like pre-salt. The currently preceding context is marked by projects guidelines designed to reduce projects and operation costs, including an important reducing in the number of people on board. The main objective of this research is to verify and discuss if the experience of use in platforms designed in a previous context, in which the people on board is practically twice, can contribute and/or can be transferred to new projects. From the ergonomic intervention in the design of two oil platform, with the work of team on board investigated on previous projects as reference, it was possible analyze if the previous use is still applicable to new projects. As a result, about 90% of the recommendations based on use are applicable to the current context. The restrictions on the transfer or operational experience are mainly related to the time of entry of ergonomics in the design process, the subsequent transformations costs and the advance of the execution phase started with the detailed design.
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Efficiency of personal protective equipment used in agriculture.
Work
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Despite the practical importance of Personal Protective Equipment (PPE), both in production and in consumption, it is observed a main problem regarding the quality of many PPE?s used in Brazil. This study found a gap between the state of the art and the state of prevailing practices in the design of PPE?s, which main consequences affect the health of workers who use PPE in agriculture activities. The results of this study identified legal constraints of this occurrence, involving the validation of PPE designed for isolate agents, without the analysis of relevant activities and without prototypes experiments in more frequent use situation.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.