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Find video protocols related to scientific articles indexed in Pubmed.
CCR7 expression and intratumoral FOXP3+ regulatory T cells are correlated with overall survival and lymph node metastasis in gastric cancer.
PLoS ONE
PUBLISHED: 01-01-2013
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The aim of this study was to investigate the prognostic value of chemokine receptor CCR7 expression and intratumoral FOXP3(+) regulatory T cells (Tregs) in gastric cancer. CCR7(+) tumor cells and FOXP3(+) Tregs were assessed by immunohistochemistry in tissue microarrays containing gastric cancer from 133 patients. Prognostic effects of low or high CCR7 and FOXP3 expression were evaluated by Cox regression and Kaplan-Meier analysis, as well as the correlation between CCR7 positive score and intratumoral FOXP3(+) cell number in a longitudinal assessment. The analysis showed that the high expression levels of CCR7 and FOXP3 were detected in 69.9% and 65.4% of cases, respectively. High CCR7 expression in gastric cancer cells was significantly associated with poor overall survival (OS) (P = 0.010) and lymph node metastasis (P = 0.009), and was an independent factor for worse OS (P = 0.023) by multivariate analysis. High numbers of intratumoral FOXP3(+) Tregs significantly correlated with shorter OS (P = 0.021) and lymph node metastasis (P = 0.024), and was also an independent factor for adverse OS (P = 0.035). Furthermore, there was a significantly positive correlation between CCR7 positive score and intratumoral FOXP3(+) cell number (r = 0.949, P<0.001). These results revealed that CCR7 expression in gastric cancer cells and intratumoral FOXP3(+) Tregs could be considered as a co-indicator of clinical prognosis of gastric cancer.
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Depletion of CD4+ CD25+ regulatory T cells promotes CCL21-mediated antitumor immunity.
PLoS ONE
PUBLISHED: 01-01-2013
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CCL21 is known to attract dendritic cells (DCs) and T cells that may reverse tumor-mediated immune suppression. The massive infiltration of tumors by regulatory T cells (Tregs) prevents the development of a successful helper immune response. In this study, we investigated whether elimination of CD4(+) CD25(+) Tregs in the tumor microenvironment using anti-CD25 monoclonal antibodies (mAbs) was capable of enhancing CCL21-mediated antitumor immunity in a mouse hepatocellular carcinoma (HCC) model. We found that CCL21 in combination with anti-CD25 mAbs (PC61) resulted in improved antitumor efficacy and prolonged survival, not only inhibited tumor angiogenesis and cell proliferation, but also led to significant increases in the frequency of CD4(+), CD8(+) T cells and CD11c(+) DCs within the tumor, coincident with marked induction of tumor-specific CD8(+) cytotoxic T lymphocytes (CTLs) at the local tumor site. The intratumoral immune responses were accompanied by the enhanced elaboration of IL-12 and IFN-?, but reduced release of the immunosuppressive mediators IL-10 and TGF-?1. The results indicated that depletion of Tregs in the tumor microenvironment could enhance CCL21-mediated antitumor immunity, and CCL21 combined with anti-CD25 mAbs may be a more effective immunotherapy to promote tumor rejection.
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Timing of gene expression from different genetic systems in shaping leucine and isoleucine contents of rapeseed (Brassica napus L.) meal.
J. Genet.
PUBLISHED: 10-13-2011
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Experiments were conducted on rapeseed (Brassica napus L.) using a diallel design with nine parents: Youcai 601, Double 20-4, Huashuang 3, Gaoyou 605, Zhongyou 821, Eyouchangjia, Zhong R-888, Tower and Zheshuang 72. The seed developmental process was divided into five stages, namely initial (days 1-15 after flowering), early (days 16-22 after flowering), middle (days 23-29), late (days 30-36), and maturing (days 37-43) developmental stages. The variation of dynamic genetic effects for leucine and isoleucine contents of rapeseed meal was analysed at five developmental stages, across different environments using the genetic models with time-dependent measures. The results from unconditional and conditional analyses indicated that the expression of diploid embryo, cytoplasmic and diploid maternal plant genes were important for leucine and isoleucine contents at different developmental stages of rapeseed, particularly at the initial and early developmental stages. Among different genetic systems, nutrition quality traits were mainly controlled by the accumulative or net maternal main effects and their GE interaction effects, except at maturity when the net diploid embryo effects were larger. The expression of genes was affected by the environmental conditions on 15, 22, 29 or 36 days after flowering, but was more stable at mature stage. For the isoleucine content the narrow-sense heritabilities on 15, 22, 29, 36, and 43 days after flowering were 43.0, 65.7, 60.1, 65.5 and 78.2%, respectively, while for the leucine content the corresponding narrow-sense heritabilities were relatively smaller. The interaction heritabilities were more important than the general heritabilities at the first three developmental times. The improvement for isoleucine content could be achieved by selection based on the higher narrow-sense heritabilities. Various genetic systems exhibited genetic correlations among the developmental times or leucine and isoleucine contents. A simultaneous improvement of leucine and isoleucine contents seems possible because of the significant positive genetic correlation components from different genetic systems at different developmental times.
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[Effect of the formulae for calming the liver and suppressing YANG on lymphocyte proteome in migraine rats with syndrome of hyperactivity of liver-YANG].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 02-05-2010
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To explore the molecular mechanism of the formulae for calming the liver and suppressing YANG in migraine rat model with syndrome of hyperactivity of the liver-YANG.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.