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Find video protocols related to scientific articles indexed in Pubmed.
Improving the carrier balance of light-emitting electrochemical cells based on ionic transition metal complexes.
Dalton Trans
PUBLISHED: 11-20-2014
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Recently, solid-state light-emitting electrochemical cells (LECs) based on ionic transition metal complexes (iTMCs) have attracted much research interest since they have the advantages of a simple device structure, a low operation voltage and compatibility with air-stable electrodes. These properties enable LECs to be cost-effective, versatile and power-efficient organic light-emitting sources. However, it is generally not easy to modify the molecular structure to achieve balanced carrier mobilities without altering the photoluminescence quantum yield of the iTMC. Furthermore, the carrier balance and the consequent device efficiency of single-layered LECs would not be easy to optimize since no carrier injection and transport layers can be used. In this perspective, some reported techniques to improve carrier balance of LECs based on iTMCs are described and reviewed. The importance and impact of these studies are highlighted. The effects on device lifetime and turn-on time because of employing these techniques to improve the carrier balance are also discussed. This perspective concludes that even with electrochemically doped layers, improving the carrier balance of LECs would be required for realizing efficient electroluminescent emission from simple-structure organic light-emitting sources.
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An ATP-responsive smart gate fabricated with a graphene oxide-aptamer-nanochannel architecture.
Chem. Commun. (Camb.)
PUBLISHED: 11-20-2014
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Here, we report a graphene oxide-aptamer-nanochannel architecture for the fabrication of a novel stimuli-responsive gate. The gate is switched OFF in the absence of ATP, and is switched ON when ATP is present. The concept we proposed may contribute to a versatile platform for the development of stimuli-responsive gates.
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Non-small cell lung cancer with EML4-ALK translocation in Chinese male never-smokers is characterized with early-onset.
BMC Cancer
PUBLISHED: 10-30-2014
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The translocations of the anaplastic lymphoma kinase (ALK) gene with the echinoderm microtubule-associated protein-like 4 (EML4) gene on chromosome 2p have been identified in non-small-cell lung cancers (NSCLCs) as oncogenic driver mutations. It has been suggested that EML4-ALK fusion is associated with the resistance in NSCLCs to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs), such as gefitinib and erlotinib. In contrast, ALK tyrosine kinase inhibitor (ALK TKI) crizotinib has shown superior effects in combating NSCLCs with EML4-ALK. Thus, characterization of EML4-ALK fusion genes and clinical features of resulting carcinomas would be a great benefit to disease diagnosis and designing customized treatment plans. Studies have suggested that EML4-ALK translocation occurs more frequently in never-smokers with NSCLC, especially in female patients. However, it is not clear whether this is the case in male patients, too. In this study, we have determined the frequency of EML4-ALK translocation in male never-smokers with NSCLC in a cohort of Chinese patients. The clinical features associated with EML4-ALK translocation were also investigated.
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Doxorubicin encapsulated in micelles enhances radiosensitivity in doxorubicin-resistant tumor cells.
Discov Med
PUBLISHED: 10-23-2014
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To evaluate the efficacy of doxorubicin (DOX) loaded micelles in enhancing DOX radiosensitivity in DOX-resistant K562 tumor cells (K562/DOX cells), DOX loaded polyethylene glycol-polycaprolactone (PEG-PCL) copolymer micelles and pluronic 105 (P105) micelles, and composite micelles composed of PEG-PCL and P105 were prepared. By using MTT assay, soft agar cloning assays, confocal laser scanning microscopy, and flow cytometry analyses to evaluate the radiosensitivity of each compound, DOX loaded micelles were found to increase the radiosensitivity of K562/DOX cells, as revealed by a marked cellular uptake and its sustained, slower release than free DOX. The micelles encapsulating DOX significantly enhanced its cytotoxicity in K562/DOX cells. Combined treatment with the encapsulation of DOX in micelles and radiotherapy therefore warrants investigation in clinical trials as a potential anticancer strategy with increased efficacy and reduced side effects.
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Positional isomeric tunable two Co(II) 6-connected 3-D frameworks with pentanuclear to binuclear units: structures, ion-exchange and magnetic properties.
Dalton Trans
PUBLISHED: 09-06-2014
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Two new Co(II) based metal-organic frameworks, namely {[Co5(?3-OH)2(m-pda)3(bix)4]·2ClO4}n (1) and {[Co2(p-pda)2(bix)2(H2O)]·H2O}n (2), were prepared by hydrothermal reactions of Co(II) salt with two isomeric dicarboxyl tectons 1,3-phenylenediacetic acid (m-pda) and 1,4-phenylenediacetic acid (p-pda), along with 1,3-bis(imidazol-L-ylmethyl)benzene (bix). Both complexes 1 and 2 have been characterized by elemental analysis, IR spectroscopy, single-crystal X-ray diffraction, powder X-ray diffraction (PXRD), and thermogravimetric analysis (TGA). 1 shows a 6-connected 3-D pcu cationic framework with pentanuclear [Co5(?3-OH)2(COO)6(bix)2](2+) units, while 2 exhibits a 6-connected 3-D msw net based on [Co2(?2-H2O)(COO)2](2+) clusters. The results indicate that the different dispositions of the carboxylic groups of dicarboxylates have an important effect on the overall coordination frameworks. Perchlorate anions in 1 can be partly exchanged by thiocyanate and azide anions, however they are unavailable to nitrate anions. Magnetic susceptibility measurements indicate that both 1 and 2 show weak antiferromagnetic interactions between the adjacent Co(II) ions.
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5,7,3',4'-Tetramethoxyflavone exhibits chondroprotective activity by targeting ?-catenin signaling in vivo and in vitro.
Biochem. Biophys. Res. Commun.
PUBLISHED: 09-01-2014
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Osteoarthritis (OA) is a progressive joint disorder, which remains the leading cause of chronic disability in aged people. This study is the first report which demonstrates the cartilage protective effect of 5,7,3',4'-tetramethoxyflavone (TMF) by decreasing the concentration of IL-1?, TNF-? and PGE2 in the knee synovial fluid in OA rat models in vivo. In vitro, after induced by PGE2, the apoptosis rate of chondrocytes was significantly increased. In addition, PGE2 increased the expression of cAMP/PKA signaling pathway in chondrocytes, stabilized and accumulated ?-catenin, and activated the expression of ?-catenin signaling pathway. These activities were counteracted by TMF dose-dependently. Collectively, TMF is a potential compound with chondroprotective activity by inhibiting both EP/cAMP/PKA signaling pathway and ?-catenin signaling pathway.
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Evaluation of PEGylated Exendin-4 Released from Poly (Lactic-co-Glycolic Acid) Microspheres for Antidiabetic Therapy.
J Pharm Sci
PUBLISHED: 07-07-2014
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Peptide-based therapies have the potential to induce antibody formation if the molecules differ from a native human peptide. Several reports have disclosed the occurrence of antibody generation in a patient treated with exenatide. The immune response can be problematic from a clinical stand point, particularly if the antibodies neutralize the efficacy of the biotherapeutic agent or cause a general immune reaction. To overcome this limit, PEGylated exendin-4 analogs were designed and examined for metabolic stability and biological activity. To develop an extended release delivery system for exendin-4 for the safe and effective delivery of bioactive exendin-4 without peptide acylation and immunogenicity, PEGylated exendin-4 was encapsulated into poly (lactic-co-glycolic acid) (PLGA) microspheres by w/o/w double emulsion solvent evaporation method. Peptide-loaded microspheres were characterized in terms of morphology, particle diameter, and peptide encapsulation efficiency. Then, the release profile of the peptide from PLGA microspheres and the acylated products from PLGA polymer degradation was determined. The results obtained showed that the stability of exendin-4 was greatly improved by PEGylation. Moreover, eliminated acylation during PLGA polymer degradation in vitro and reduced immunogenicity in vivo were observed. The findings demonstrate that PEGylated exendin-4-loaded microspheres may be a safe and biocompatible system for clinical development. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci.
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Complementary sequence-mediated exon circularization.
Cell
PUBLISHED: 07-01-2014
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Exon circularization has been identified from many loci in mammals, but the detailed mechanism of its biogenesis has remained elusive. By using genome-wide approaches and circular RNA recapitulation, we demonstrate that exon circularization is dependent on flanking intronic complementary sequences. Such sequences and their distribution exhibit rapid evolutionary changes, showing that exon circularization is evolutionarily dynamic. Strikingly, exon circularization efficiency can be regulated by competition between RNA pairing across flanking introns or within individual introns. Importantly, alternative formation of inverted repeated Alu pairs and the competition between them can lead to alternative circularization, resulting in multiple circular RNA transcripts produced from a single gene. Collectively, exon circularization mediated by complementary sequences in human introns and the potential to generate alternative circularization products extend the complexity of mammalian posttranscriptional regulation.
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[Transplanted vascular endothelial cells to replace corneal endothelial cells by improved anterior chamber injection].
Zhonghua Yan Ke Za Zhi
PUBLISHED: 06-17-2014
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To observe form and function changes of vascular endothelial cells (VEC) which were transplanted to the posterior surface of rhesus monkey cornea without Descemet's Membrane by anterior chamber injection, explore the feasibility of transplanting VEC to treat corneal endothelial injury, and find new method of corneal endothelial cell transplantation.
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[Inhibition of Hep-2 cell apoptosis after annexin A5 knockdown].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 06-17-2014
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To study the effect of annexin A5 on the apoptosis of laryngeal cancer cells.
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[Spectral characterization for capsanthin molecule with in vitro oxidative defense reaction].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-15-2014
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The changes of UV-Vis and FTIR spectroscopic properties for capsanthin before and after reaction with exogenous superoxide anion (*O2(-)), hydrogen peroxide (H2O2) and hydroxyl radical (*OH), catalase (CAT), peroxidase (POD) and lipoxygenase (LOX) were explored. The results showed that, the UV-Vis spectral absorption of capsanthin treated with reactive oxygen species had a blue-shift. At the same time, the FTIR spectra changed significantly. The number of FTIR spectral peaks reduced and theFTIR strength weakened for capsanthin molecule treated with *O2(-) and *OH. The characteristic and strong peaks moved to shorter wavelengths when treated with H2O2. And LOX caused breakage of capsanthin molecule and reduction of peak number or groups without carbonyl. Exogenous H2O2 + CAT or H2O2 + POD treatment could not affect the UV-Vis and FTIR spectra significantly. So ROS could cause oxidative degradation of capsanthin and destroy chromophoric groups such as carbon-carbon double bond and carbonyl, then grow colorless alcohols. Hence ROS and LOX should transforms the conjugate system of capsanthin molecules, while CAT and POD could protect the capsanthin.
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Do Ni/Cu and Cu/Ni alloys have different catalytic performances towards water-gas shift? A density functional theory investigation.
Chemphyschem
PUBLISHED: 04-30-2014
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Density functional calculations were preformed to investigate whether adding Ni into a Cu surface (denoted as Cu/Ni) or adding Cu into a Ni surface (Ni/Cu) is more efficient for catalyzing the water-gas shift (WGS)? The reactions of water dissociation and monoxide dissociation were selected to assess the activity and selectivity towards WGS, respectively. Our results show that Ni-atom modification of surfaces is thermodynamically favorable for both reactions. Kinetically, compared with pure Cu, water dissociation is greatly facilitated on Ni-modified surfaces, and the activity is insensitive to the Ni concentration; however, monoxide dissociation is not well-promoted on one Ni-atom-modified surfaces, but two Ni-atom modification can notably decrease the dissociation barriers. Overall, on the basis of these results, we conclude that 1) the catalytic performance of bimetallic metals is superior to monometallic ones; 2) at the same Ni concentration on the surface, Cu/Ni and Ni/Cu alloys have almost the same performance towards WGS; and 3) to acquire high WGS performance, the surface Ni atoms should either be low in concentration or highly dispersed.
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Cadmium sulfide quantum dots supported on gallium and indium oxide for visible-light-driven hydrogen evolution from water.
ChemSusChem
PUBLISHED: 04-21-2014
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In this work, CdS quantum dots (QDs) supported on Ga2O3 and In2O3 are applied for visible-light-driven H2 evolution from aqueous solutions that contain lactic acid. With Pt as the cocatalyst, the H2 evolution rates on CdS/Pt/Ga2O3 and CdS/Pt/In2O3 are as high as 995.8 and 1032.2??mol?h(-1), respectively, under visible light (?>420?nm) with apparent quantum efficiencies of 43.6 and 45.3% obtained at 460?nm, respectively. These are much higher than those on Pt/CdS (108.09??mol?h(-1)), Pt/Ga2O3 (0.12??mol?h(-1)), and Pt/In2O3 (0.05??mol?h(-1)). The photocatalysts have been characterized thoroughly and their band structures and photocurrent responses have been measured. The band alignment between the CdS QDs and In2O3 can lead to interfacial charge separation, which cannot occur between the CdS QDs and Ga2O3. Among the various possible factors that contribute to the high H2 evolution rates on CdS/Pt/oxide, the surface properties of the metal oxides play important roles, which include (i)?the anchoring of CdS QDs and Pt nanoparticles for favorable interactions and (ii)?the efficient trapping of photogenerated electrons from the CdS QDs because of surface defects (such as oxygen defects) based on photoluminescence and photocurrent studies.
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Long term outcomes of laparoscopic-assisted anorectoplasty: a comparison study with posterior sagittal anorectoplasty.
J. Pediatr. Surg.
PUBLISHED: 04-15-2014
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The aim of this study is to compare the long term outcomes between laparoscopic-assisted anorectoplasty (LAARP) and posterior sagittal anorectoplasty (PSARP) for children with rectobladderneck and rectoprostatic fistula anorectal malformations (ARM).
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Fuzheng Huayu recipe alleviates hepatic fibrosis via inhibiting TNF-alpha induced hepatocyte apoptosis.
BMC Complement Altern Med
PUBLISHED: 04-08-2014
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What was the relationship of Fuzheng Huayu recipe (FZHY) inhibiting hepatocyte apoptosis and HSC activation at different stage of liver fibrosis? In order to answer this question, the study was carried out to dynamically observe FZHY's effect on hepatocyte apoptosis and HSC activation and further explored underling mechanism of FZHY against hepatocyte apoptosis.
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Racial differences in sensitivity of blood pressure to aldosterone.
Hypertension
PUBLISHED: 04-07-2014
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Blacks in comparison with whites are at risk for a more serious form of hypertension with high rates of complications. Greater sodium retention is thought to underlie the blood pressure (BP)-determining physiology of blacks, but specific mechanisms have not been identified. In a prospective observational study of BP, 226 black children and 314 white children (mean age, 10.6 years) were enrolled initially. Assessments were repeated in 85 blacks and 136 whites after reaching adulthood (mean age, 31 years). The relationship of BP to plasma aldosterone concentration in the context of the prevailing level of plasma renin activity was studied in blacks and whites. In a secondary interventional study, 9-? fludrocortisone was administered for 2 weeks to healthy adult blacks and whites to simulate hyperaldosteronism. BP responses in the 2 race groups were then compared. Although black children had lower levels of plasma renin activity and plasma aldosterone, their BP was positively associated with the plasma aldosterone concentration, an effect that increased as plasma renin activity decreased (P=0.004). Data from black adults yielded similar results. No similar relationship was observed in whites. In the interventional study, 9-? fludrocortisone increased BP in blacks but not in whites. In conclusion, aldosterone sensitivity is a significant determinant of BP in young blacks. Although its role in establishing the risk of hypertension is not known, it could be as relevant as the actual level of aldosterone.
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Species-specific alternative splicing leads to unique expression of sno-lncRNAs.
BMC Genomics
PUBLISHED: 04-03-2014
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Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs from other regions of the human genome or from other genomes have not yet been documented.
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GaAs/AlGaAs nanowire photodetector.
Nano Lett.
PUBLISHED: 04-03-2014
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We demonstrate an efficient core-shell GaAs/AlGaAs nanowire photodetector operating at room temperature. The design of this nanoscale detector is based on a type-I heterostructure combined with a metal-semiconductor-metal (MSM) radial architecture, in which built-in electric fields at the semiconductor heterointerface and at the metal/semiconductor Schottky contact promote photogenerated charge separation, enhancing photosensitivity. The spectral photoconductive response shows that the nanowire supports resonant optical modes in the near-infrared region, which lead to large photocurrent density in agreement with the predictions of electromagnetic and transport computational models. The single nanowire photodetector shows a remarkable peak photoresponsivity of 0.57 A/W, comparable to large-area planar GaAs photodetectors on the market, and a high detectivity of 7.2 × 10(10) cm·Hz(1/2)/W at ? = 855 nm. This is promising for the design of a new generation of highly sensitive single nanowire photodetectors by controlling the optical mode confinement, bandgap, density of states, and electrode engineering.
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Electroacupuncture at Zusanli (ST36) promotes gastric emptying and mucosal blood flow during oral resuscitation of scalded rats with a pyruvate-enriched ORS.
Burns
PUBLISHED: 04-02-2014
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The aim of this study was to investigate the effect of electroacupuncture at ST36 (EA ST36) on gastric emptying and mucosal blood flow during intragastric resuscitation with pyruvate-enriched oral rehydration solution (Pyr-ORS) in scalded rats.
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Comparative expression profiles of microRNA in left and right atrial appendages from patients with rheumatic mitral valve disease exhibiting sinus rhythm or atrial fibrillation.
J Transl Med
PUBLISHED: 04-02-2014
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The atrial fibrillation (AF) associated microRNAs (miRNAs) were found in the right atrium (RA) and left atrium (LA) from patients with rheumatic mitral valve disease (RMVD). However, most studies only focus on the RA; and the potential differences of AF-associated miRNAs between the RA and LA are still unknown. The aim of this study was to perform miRNA expression profiles analysis to compare the potential differences of AF-associated miRNAs in the right atrial appendages (RAA) and left atrial appendages (LAA) from RMVD patients.
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A two-in-one Faraday rotator mirror exempt of active optical alignment.
Opt Express
PUBLISHED: 03-26-2014
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A two-in-one Faraday rotator mirror was presented, which functions as two independent Faraday rotation mirrors with a single device. With the introduction of a reflection lens as substitution of the mirror in traditional structure, this device is characterized by exemption of active optical alignment for the designers and manufacturers of Faraday rotator mirrors. A sample was fabricated by passive mechanical assembly. The insertion loss was measured as 0.46 dB/0.50 dB for the two independent ports, respectively.
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Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus.
Cell Res.
PUBLISHED: 03-25-2014
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The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how chromatin looping at the MYC locus is regulated remains poorly understood. Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. Importantly, the CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC. Knockdown of CCAT1-L reduced long-range interactions between the MYC promoter and its enhancers. In addition, CCAT1-L interacts with CTCF and modulates chromatin conformation at these loop regions. These results reveal an important role of a previously unannotated lncRNA in gene regulation at the MYC locus.
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Identification and pharmacokinetics of multiple constituents in rat plasma after oral administration of Yinchenzhufu decoction.
J Ethnopharmacol
PUBLISHED: 03-14-2014
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Yinchenzhufu decoction (YCZFD) is a classical Chinese herbal formula and has been used to treat severe jaundice in chronic liver injuries since the Qing Dynasty (18th century CE). To identify the components absorbed into the blood in YCZFD and explore their pharmacokinetic profile for understanding the effective ingredients of YCZFD.
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Fast-track surgery combined with laparoscopy could improve postoperative recovery of low-risk rectal cancer patients: a randomized controlled clinical trial.
J Dig Dis
PUBLISHED: 03-07-2014
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In this study we aimed to assess the feasibility and safety of fast-track surgery (FTS) combined with laparoscopy for treating patients with rectal cancer and compare the results with those of the conventional perioperative intervention group.
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Effect of whole body vibration therapy on circulating serotonin levels in an ovariectomized rat model of osteoporosis.
Iran J Basic Med Sci
PUBLISHED: 03-05-2014
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Studies have reported that whole body vibration (WBV) played a vital role in bone remodeling. Circulating serotonin is also involved in negative regulating bone mass in rodents and humans. However, both WBV and inhibition of serotonin biosynthesis may suppress receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis in vitro. The purpose of the current study was to investigate the effect of WBV therapy on the levels of serum serotonin in ovariectomized rats.
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Mitochondrial pathology in osteoarthritic chondrocytes.
Curr Drug Targets
PUBLISHED: 03-02-2014
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Osteoarthritis (OA) is a chronic degenerative disease leading to aberrance of cartilage structures with unclear or multifactorial mechanisms. Recently, a great portion of research endeavor to explore the molecular mechanisms of OA in focusing on the mitochondrial pathology. Mitochondrial respiratory chain (MRC) produces reactive oxygen species (ROS), which in turn impair mtDNA integrity and link to cartilage degradation in OA. The fine-tuning between ROS and antioxidant within chondrocytes ensures cartilage homeostasis. With disturbance from pro-inflammatory cytokines, oxidative stress synergistically instigates cellular signaling and exacerbates mitochondrial pathology, which may affect several pathways implicated in OA cartilage degradation, including oxidative stress, increase of cytokine-induced chondrocytes inflammation and matrix catabolism, aging and senescence, obesity-related pathology, and cartilage matrix calcification. Unveiling the molecular mechanisms of mitochondrial function in OA pathogenesis and progression is essential for providing relevant therapeutic targets. These suggest that efficient protection and improvement of mitochondrial activity can be a therapeutic alternative for OA patients.
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The role of metal layers in the formation of metal-silicon hybrid nanoneedle arrays.
Nanoscale
PUBLISHED: 02-25-2014
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We investigated nanoneedle arrays fabricated on a series of metal-silicon substrates using Ga(+) ion beam patterning. It is shown that the low sputtering rate of the metal is preserved on the tip of each nanoneedle in the form of a gallium alloy nanodot. The generated nanodot was found to greatly alleviate the ion sputtering of the underlying materials. These protective metals are promising materials that act as a shelter for the functional layer, which is vulnerable to ion beam irradiation. In the present work, as an example, we report a bundle of GaAs nanowhiskers that were successfully grown on each gold nanodot protected by an iron-gallium alloy.
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Transition-metal-ion-mediated polymerization of dopamine: mussel-inspired approach for the facile synthesis of robust transition-metal nanoparticle-graphene hybrids.
Chemistry
PUBLISHED: 02-18-2014
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Inspired by the high transition-metal-ion content in mussel glues, and the cross-linking and mechanical reinforcement effects of some transition-metal ions in mussel threads, high concentrations of nickel(II), cobalt(II), and manganese(II) ions have been purposely introduced into the reaction system for dopamine polymerization. Kinetics studies were conducted for the Ni(2+)-dopamine system to investigate the polymerization mechanism. The results show that the Ni(2+) ions could accelerate the assembly of dopamine oligomers in the polymerization process. Spectroscopic and electron microscopic studies reveal that the Ni(2+) ions are chelated with polydopamine (PDA) units, forming homogeneous Ni(2+)-PDA complexes. This facile one-pot approach is utilized to construct transition-metal-ion-PDA complex thin coatings on graphene oxide, which can be carbonized to produce robust hybrid nanosheets with well-dispersed metallic nickel/metallic cobalt/manganese(II) oxide nanoparticles embedded in PDA-derived thin graphitic carbon layers. The nickel-graphene hybrid prepared by using this approach shows good catalytic properties and recyclability for the reduction of p-nitrophenol.
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Posttraumatic stress disorder and posttraumatic growth among adult survivors of Wenchuan earthquake after 1 year: prevalence and correlates.
Arch Psychiatr Nurs
PUBLISHED: 02-11-2014
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This study investigates the prevalence and predictors for posttraumatic stress disorder (PTSD) and posttraumatic growth (PTG) in adult survivors 1year after the 2008 Wenchuan earthquake. Questionnaires were used to collect the data. PTSD was assessed using the PTSD Check List-Civilian (PCL-C), and PTG was assessed using the Post Traumatic Growth Inventory (PTGI). A total of 2,300 individuals were involved in the survey with 2,080 completing the questionnaire, a response rate of 90.4%. The PTSD prevalence estimate in this study was found to be 40.1%, and the prevalence for PTG among the participants was measured at 51.1%. A bivariate correlation analysis indicated that there was a positive association between PTG and PTSD. In the conclusions, possible explanations for the findings and implications for future research are discussed.
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Bayesian analysis of perfusion-weighted imaging to predict infarct volume: comparison with singular value decomposition.
Magn Reson Med Sci
PUBLISHED: 01-31-2014
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We compared the performances of a Bayesian estimation method and oscillation index singular value decomposition (oSVD) deconvolution for predicting final infarction using data previously obtained from 10 cynomolgus monkeys with permanent unilateral middle cerebral artery (MCA) occlusion.
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HDAC inhibitors reverse acquired radio resistance of KYSE-150R esophageal carcinoma cells by modulating Bmi-1 expression.
Toxicol. Lett.
PUBLISHED: 01-25-2014
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Tumors treated with fractionated doses of ionizing radiation (IR) often acquire radioresistance. Although histone deacetylase inhibitors (HDIs) have been demonstrated to sensitize intrinsic radioresistant cancer cell lines to IR, little is known on the impact of HDIs on the effects of IR in acquired radioresistant cancer cells. This study evaluates the mechanisms by which HDIs sensitize acquired radioresistant esophageal squamous cell carcinoma cells to IR. The HDIs trichostatin A and sodium butyrate were tested for the irability to sensitize acquired radioresistant KYSE-150R and radiosensitive KYSE-150 parental cells to IR. Although the HDIs induced similar levels of cytotoxicity in the KYSE-150 and the KYSE-150R cells, HDIs increased the: (i) radiosensitivity, (ii) IR-induced ROS generation, and (iii) IR-induced G2/M arrest and apoptosis of KYSE-150R cells compared with those of KYSE-150 cells. These changes were accompanied by increased p21 expression and decreased mitochondrial membrane potential. When combined with IR, HDIs inhibited Bmi-1 expression in KYSE-150R cells and their ability to repair DNA damage. The results demonstrate the potential utility of HDIs in augmenting the efficacy of fractionated radiotherapy.
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Atrial fibrillation alters the microRNA expression profiles of the left atria of patients with mitral stenosis.
BMC Cardiovasc Disord
PUBLISHED: 01-22-2014
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Structural changes of the left and right atria associated with atrial fibrillation (AF) in mitral stenosis (MS) patients are well known, and alterations in microRNA (miRNA) expression profiles of the right atria have also been investigated. However, miRNA changes in the left atria still require delineation. This study evaluated alterations in miRNA expression profiles of left atrial tissues from MS patients with AF relative to those with normal sinus rhythm (NSR).
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Factors affecting quantity of pollen dispersal of spray cut chrysanthemum (Chrysanthemum morifolium).
BMC Plant Biol.
PUBLISHED: 01-02-2014
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Spray cut chrysanthemum is a vital flower with high ornamental value and popularity in the world. However, the excessive quantity of pollen dispersal of most spray cut chrysanthemum is an adverse factor during its flowering stage, and can significantly reduce its ornamental value and quickly shorten its vase life. More seriously, excessive pollen grains in the air are usually harmful to people, especially for those with pollen allergies. Therefore, in order to obtain some valuable information for developing spray cut chrysanthemum with less-dispersed or non-dispersed pollen in the future breeding programs, we here investigated the factors affecting quantity of pollen dispersal of spray cut chrysanthemum with four cultivars, i.e. 'Qx-097', 'Noa', 'Qx-115', and 'Kingfisher', that have different quantity of pollen dispersal.
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Analysis of transcription factor network underlying 3T3-L1 adipocyte differentiation.
PLoS ONE
PUBLISHED: 01-01-2014
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Lipid accumulation in adipocytes reflects a balance between enzymatic pathways leading to the formation and breakdown of esterified lipids, primarily triglycerides. This balance is extremely important, as both high and low lipid levels in adipocytes can have deleterious consequences. The enzymes responsible for lipid synthesis and breakdown (lipogenesis and lipolysis, respectively) are regulated through the coordinated actions of several transcription factors (TFs). In this study, we examined the dynamics of several key transcription factors (TFs) - PPAR?, C/EBP?, CREB, NFAT, FoxO1, and SREBP-1c - during adipogenic differentiation (week 1) and ensuing lipid accumulation. The activation profiles of these TFs at different times following induction of adipogenic differentiation were quantified using 3T3-L1 reporter cell lines constructed to secrete the Gaussia luciferase enzyme upon binding of a TF to its DNA binding element. The dynamics of the TFs was also modeled using a combination of logical gates and ordinary differential equations, where the logical gates were used to explore different combinations of activating inputs for PPAR?, C/EBP?, and SREBP-1c. Comparisons of the experimental profiles and model simulations suggest that SREBP-1c could be independently activated by either insulin or PPAR?, whereas PPAR? activation required both C/EBP? as well as a putative ligand. Parameter estimation and sensitivity analysis indicate that feedback activation of SREBP-1c by PPAR? is negligible in comparison to activation of SREBP-1c by insulin. On the other hand, the production of an activating ligand could quantitatively contribute to a sustained elevation in PPAR? activity.
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[Dynamic analysis of the lymphocyte subsets in HCV children with different genotypes during treatment].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
PUBLISHED: 12-11-2013
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To discuss the changes of lymphocyte subsets in HCV children with different genotypes during treatment with pegylated interferon alfa-2b and ribavirin.
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Robust destriping method with unidirectional total variation and framelet regularization.
Opt Express
PUBLISHED: 10-10-2013
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Multidetector imaging systems often suffer from the problem of stripe noise and random noise, which greatly degrade the imaging quality. In this paper, we propose a variational destriping method that combines unidirectional total variation and framelet regularization. Total-variation-based regularizations are considered effective in removing different kinds of stripe noise, and framelet regularization can efficiently preserve the detail information. In essence, these two regularizations are complementary to each other. Moreover, the proposed method can also efficiently suppress random noise. The split Bregman iteration method is employed to solve the resulting minimization problem. Comparative results demonstrate that the proposed method significantly outperforms state-of-the-art destriping methods on both qualitative and quantitative assessments.
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Survey of the total fatty acid and triacylglycerol composition and content of 30 duckweed species and cloning of a ?6-desaturase responsible for the production of ?-linolenic and stearidonic acids in Lemna gibba.
BMC Plant Biol.
PUBLISHED: 10-03-2013
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Duckweeds, i.e., members of the Lemnoideae family, are amongst the smallest aquatic flowering plants. Their high growth rate, aquatic habit and suitability for bio-remediation make them strong candidates for biomass production. Duckweeds have been studied for their potential as feedstocks for bioethanol production; however, less is known about their ability to accumulate reduced carbon as fatty acids (FA) and oil.
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[Effects of cordyceps acid and cordycepin on the inflammatory and fibrogenic response of hepatic stellate cells].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 09-12-2013
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To investigate the effects of cordyceps acid and cordycepin on the inflammatory phenotype and fibrogenic property of hepatic stellate cells (HSCs).
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Salvianolic acid B inhibits hepatic stellate cell activation through transforming growth factor beta-1 signal transduction pathway in vivo and in vitro.
Exp. Biol. Med. (Maywood)
PUBLISHED: 09-04-2013
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Salvianolic acid B (Sal B) is a major water soluble component extracted from Radix Salviae miltiorrhizae, a traditional Chinese herb widely used for treating cardiovascular and hepatic diseases. Sal B has been reported to inhibit transforming growth factor (TGF)-?1-stimulated hepatic stellate cells (HSCs) activation and collagen type I expression. In this study, we further investigated the mechanisms of Sal B on liver fibrosis relating to TGF-?/Smads signalling pathway, especially to TGF-?1 receptors. Liver fibrosis model was induced by intraperitoneal injection of dimethylnitrosamine (DMN) for four weeks. Rats were randomly divided into three groups: normal, model, and Sal B groups. Rats in Sal B group were treated by oral administration of Sal B for four weeks from the first day of DMN exposure. Hydroxyproline (Hyp) content in liver tissue was assayed using Jamalls method and collagen deposition was visualized using Sirius red staining. HSCs were isolated from normal rats, and were cultured primarily in uncoated plastics. At day 4 after isolation, cells were stimulated with 2.5?ng/mL TGF-?1, and treated with 1 and 10?µmol/L Sal B and 10?µmol/L SB-431542 (T?R-I inhibitor) for 24?h, respectively. Cell proliferation was examined with 5-ethynyl-2-deoxyuridine assay. The expressions of alpha smooth muscle actin (?-SMA) and Smad3 were assayed by immunofluorescent stain and Western blotting. The expression of T?R-I was analysed by Western blotting and real-time polymerase chain reaction. The activity of T?R-I kinase was measured by ADP-Glo kinase assay. The results showed that Sal B could inhibit collagen deposition and reduce Hyp content significantly, and decrease expressions of TGF-?1 and T?R-I in fibrotic liver in vivo. Also, Sal B decreased the expressions of ?-SMA and T?R-I, inhibited Smad3 nuclear translocation and down-regulated T?R-I kinase activity in vitro. These findings suggested that Sal B could prevent HSCs activation through TGF-? signalling pathway, i.e. inhibiting TGF-?1 expression, activity of T?R-I kinase and Smads phosphorylation.
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[Protective effect of Tanreqing injection on acute hepatic injury induced by CCl4 in rats].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 08-16-2013
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To observe the protective effect of Tanreqing injection(TRQ) on carbon tetrachloride-induced acute hepatic injury in rats.
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Preparation and photoluminescence properties of europium ions doped TiO2 nanocrystals.
J Nanosci Nanotechnol
PUBLISHED: 08-02-2013
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In this paper, pure and Eu3+ doped TiO2 nanocrystals (NCs) have been fabricated successfully by a two steps of sol-gel and hydrothermal methods. The microstructures, morphologies and photoluminescent properties of Eu(3+)-TiO2 were investigated by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and photoluminescence spectroscopy (PL). XRD and PL results show that the existent of rare earth can inhibit the transformation of TiO2 NCs from anatase to rutile phase and can improve the luminescence intensity of the prepared samples. The room-temperature PL emission of the as-grown samples is dominated by the 5D(0)-7F(j) transitions of Eu3+ ions. But the luminescence intensity drops dramatically when the annealing temperature reaches a relatively high degree because of the formation of the rutile phase of TiO2 NCs hosts.
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Preparation, photoluminescence and photocatalytic properties of ZnO:Eu3+ nanocrystals.
J Nanosci Nanotechnol
PUBLISHED: 08-02-2013
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The ZnO short nanorods and flowerlike nanostructures doped with Eu3+ ions are prepared by a hydrothermal method through adjusting the hydrothermal temperature. The XRD, FESEM, FT-IR, luminescent and photocatalytic properties are characterized. The results indicate that the hydrothermal temperature affects the morphology of ZnO:Eu3+ nanocrystals, short nanorods at 120 degrees C, flowerlike nanocrystals at 150 degrees C and nanoparticles at 180 degrees C. The structures of ZnO:Eu3+ nanocrystals prepared at different hydrothermal temperatures are assigned to hexagonal phase. The photoluminescent properties including excitation and emission spectra are studied. The charge transfer band and f-f transition absorption are observed in excitation spectra. And, the 5D(0)-7F(J) (J = 0-4) transition emissions appear in emission spectra. In addition, the photocatalytic properties are also studied. The degradation rate of ZnO:Eu3+ nanocrystals is a little lower than pure ZnO nanocrystals.
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The siRNA cocktail targeting VEGF and HER2 inhibition on the proliferation and induced apoptosis of gastric cancer cell.
Mol. Cell. Biochem.
PUBLISHED: 07-18-2013
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The aim of this study was to investigate the inhibitory effect of a siRNA cocktail targeting Vascular endothelial growth factor (VEGF) and Human epidermal growth factor receptor 2 (HER2) on cell proliferation, induced apoptosis and the expression of VEGF and HER2 in human gastric carcinoma cell. The silencing rate of pre-designed siRNAs that targeted VEGF and HER2 was detected by Real-time Quantitative PCR (RT-QPCR) analysis. Furthermore, the best silencing siRNA that targeted VEGF and HER2 was prepared as a cocktail to co-knockdown VEGF and HER2 expression at both mRNA and protein levels which were detected by RT-QPCR and Western blot analysis. Cell proliferation inhibition rates were determined by CCK8 assay. The effect of siRNA cocktail on cell apoptosis was determined by flow cytometry. The migration inhibition of siRNA cocktail was analyzed by wound-healing assay. The ability of VEGF to induce endothelial cells to proliferate was examined in HUVECs by the method of tube formation assay. The pre-designed siRNAs could inhibit VEGF and HER2 mRNA level. siRNA cocktail, and co-downregulation of VEGF and HER2 result in significant inhibition of gastric cancer growth and migration in vitro. The inhibition of VEGF and HER2 expressions can induce apoptosis of SGC-7901 cells.
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Site-specific PEGylation of a mutated-cysteine residue and its effect on tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL).
Biomaterials
PUBLISHED: 07-17-2013
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Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a promising antitumor agent that specifically induces apoptosis in broad-spectrum tumor cell lines, meanwhile leaving normal cells unaffected. Unfortunately, the clinical development of TRAIL was hampered, and could be attributed to its instability, bioavailability or poor delivery. Although N-terminal specific PEGylation provides a means to improve the pharmacokinetic and stability of TRAIL, it took a bit longer time to accomplish the PEGylation process than expected. We therefore designed another PEGylation approach, mutated Cys-SH site-specific PEGylation, to conjugate methoxypoly(ethylene glycol) maleimide (mPEG-MAL) with TRAIL (95-281) mutant N109C. Asn-109 was chosen as the PEGylated site for it is a potential N-linked glycosylation site. It was shown that ~90% TRAIL mutant N109C could be PEGylated by mPEG-MAL within 40 min. And mPEG(MAL)-N109C was revealed to possess superior in vitro stability and antitumor activity than N-terminal specifically PEGylated TRAIL (114-281) (mPEG(ALD)-TRAIL(114-281)). Whats more, mPEG(MAL)-N109C exhibited more therapeutic potentials than mPEG(ALD)-TRAIL(114-281) in tumor xenograft model, benefitting from better drug delivery and bioavailability. These results have demonstrated mutated Cys-SH specific PEGylation is an alternative to site-specifically PEGylate TRAIL efficiently and effectively other than N-terminal specific PEGylation.
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STAT3-mediated attenuation of CCl4-induced mouse liver fibrosis by the protein kinase inhibitor sorafenib.
J. Autoimmun.
PUBLISHED: 07-12-2013
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There have been major advances in defining the immunological events associated with fibrosis in various chronic liver diseases. We have taken advantage of this data to focus on the mechanisms of action of a unique multi-kinase inhibitor, coined sorafenib, on CCl4-induced murine liver fibrosis, including the effects of this agent in models of both acute and chronic CCl4-mediated pathology. Importantly, sorafenib significantly attenuated chronic liver injury and fibrosis, including reduction in liver inflammation and histopathology as well as decreased expression of liver fibrosis-related genes, including ?-smooth muscle actin, collagen, matrix metalloproteinases and the tissue inhibitor of metalloproteinase-1. Furthermore, sorafenib treatment resulted in translocation of cytoplasmic STAT3 to the nucleus in its active form. Based on this observation, we used hepatocyte-specific STAT3 knockout (STAT3(Hep-/-)) mice to demonstrate that hepatic STAT3 was critical for sorafenib-mediated protection against liver fibrosis, and that the upregulation of STAT3 phosphorylation was dependent on Kupffer cell-derived IL-6. In conclusion, these data reflect the clinical potential of the multi-kinase inhibitor sorafenib for the prevention of fibrosis as well as the treatment of established liver fibrosis and illustrate the immunological mechanisms that underlie the protective effects of sorafenib.
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Curcumin inhibits lung cancer progression and metastasis through induction of FOXO1.
Tumour Biol.
PUBLISHED: 06-17-2013
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Recent population studies provide clues that the use of curcumin may be associated with reduced incidence and improved prognosis of certain cancers. In the present study, we demonstrated that curcumin acted as a growth inhibitor for lung cancer cells. Our results found that curcumin inhibited cell proliferation, which was associated with upregulation of the cyclin-dependent kinase inhibitors, p27 and p21, and downregulation of cyclin D1. In addition, we showed that curcumin induced the expression of forkhead box protein O1 (FOXO1) through activation of extracellular signal-regulated kinase 1/2 signaling. These findings provide evidence for a mechanism that may contribute to the antineoplastic effects of curcumin and justify further work to explore potential roles for activators of FOXO1 in the prevention and treatment of lung cancer.
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Towards Perfectly Ordered Novel ZnO/Si Nano-Heterojunction Arrays.
Small
PUBLISHED: 05-30-2013
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The fabrication of a highly ordered novel ZnO/Si nano-heterojuntion array is introduced. ZnO seed layer is first deposited on the Si (P<111>) surface. The nucleation sites are then defined by patterning the surface through focused ion beam (FIB) system. The ZnO nanorods are grown on the nucleation sites through hydrothermal process. The whole fabrication process is simple, facile and offers direct control of the space, length and aspect ratio of the array. It is found that ZnO/Si nanojunctions show an improved interface when subjected to heat treatment. The recrystallization of ZnO and the tensile lattice strain of Si developed during the heating process contribute the enhancement of their photoresponses to white light. The photoluminescence (PL) measurement result of nano-heterojunction arrays with different parameters is discussed.
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Receptor for advanced glycation end products involved in lung ischemia reperfusion injury in cardiopulmonary bypass attenuated by controlled oxygen reperfusion in a canine model.
ASAIO J.
PUBLISHED: 05-07-2013
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Controlled oxygen reperfusion could protect the lung against ischemia-reperfusion injury in cardiopulmonary bypass (CPB) by downregulating high mobility group box 1 (HMGB1), a high affinity receptor of HMGB1. This study investigated the effect of controlled oxygen reperfusion on receptor for advanced glycation end products (RAGE) expression and its downstream effects on lung ischemia-reperfusion injury. Fourteen canines received CPB with 60 minutes of aortic clamping and cardioplegic arrest followed by 90 minutes of reperfusion. Animals were randomized to receive 80% FiO2 during the entire procedure (control group) or to a test group receiving a controlled oxygen reperfusion protocol. Pathologic changes in lung tissues, RAGE expression, serum interleukin-6 (IL-6), and tumor necrosis factor-? (TNF-?) were evaluated. The lung pathologic scores after 25 and 90 minutes of reperfusion were significantly lower in the test group compared with the control group (p < 0.001). RAGE expression, TNF-?, and IL-6 were downregulated by controlled oxygen treatment (p < 0.001). RAGE might be involved in the lung ischemia-reperfusion injury in canine model of CPB, which was downregulated by controlled oxygen reperfusion.
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Optimal blood pressure for cognitive function: findings from an elderly African-American cohort study.
J Am Geriatr Soc
PUBLISHED: 05-06-2013
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To report the results from a prospective cohort study on the association between blood pressure (BP) and cognitive function in elderly African Americans.
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Investigation of the absorbed and metabolized components of Danshen from Fuzheng Huayu recipe and study on the anti-hepatic fibrosis effects of these components.
J Ethnopharmacol
PUBLISHED: 05-04-2013
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Fuzheng Huayu recipe (FZHY) was formulated on the basis of Chinese medicine theory in treating liver fibrosis. It has a significant efficacy against liver fibrosis caused by chronic hepatitis B, with the action mechanisms of inhibition of hepatic stellate cell activation, protection of hepatocyte oxidative injury and regulations of hepatic matrix remodeling etc.
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VEGF165 modulates proliferation, adhesion, migration and differentiation of cultured human outer root sheath cells from central hair follicle epithelium through VEGFR-2 activation in vitro.
J. Dermatol. Sci.
PUBLISHED: 04-17-2013
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The functional state of vasculature is tightly controlled by vascular endothelial growth factor receptor-2 (VEGFR-2). Recent studies revealed that VEGFR-2 is expressed on hair follicle keratinocytes.
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A hybrid nanostructure array for gas sensing with ultralow field ionization voltage.
Nanotechnology
PUBLISHED: 04-03-2013
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We fabricate a unique hybrid nanostructure array for gas sensing based on the polarization mechanism at the nanoscale. It is shown that with platinum nanocrystallites on the top of each nanoneedle, this array can work at ultralow voltages (less than 10 V) as a field ionization gas sensor. We believe that the polarized platinum brings about a local enhanced electrical field, leading to the direct field ionization of gas molecules, which is confirmed by calculations of the charge accumulation and electrical field distribution.
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The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury.
J Transl Med
PUBLISHED: 03-31-2013
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MicroRNAs were enrolled in various cardiovascular disease especially ischemic heart diseases, but the microRNA changes during myocardial ischemia reperfusion injury underwent cardiopulmonary bypass are still unknown. This study screens the microRNA differences in CPB canines and evaluates the relationship of microRNAs with myocardial ischemia reperfusion injury.
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Novel conjugation of tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL) with monomethyl auristatin E for efficient antitumor drug delivery.
Adv. Mater. Weinheim
PUBLISHED: 03-27-2013
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Monomethyl auristatin E (MMAE) is conjugated with TNF-related apoptosis-inducing ligand (TRAIL) via a linker that is stable in extracellular fluid, while it is cleaved by cathepsin once the conjugate has entered a tumor cell, thus activating the antimitotic mechanism of MMAE. The TRAIL-MMAE conjugate is a conceptually viable therapeutic strategy with improved in vitro antitumor activity, cell circle arrest and specific accumulation in tumor to treat TRAIL-resistant tumors.
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Fast-track surgery could improve postoperative recovery in radical total gastrectomy patients.
World J. Gastroenterol.
PUBLISHED: 03-20-2013
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To assess the impact of fast-track surgery (FTS) on hospital stay, cost of hospitalization and complications after radical total gastrectomy.
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Adiposity has unique influence on the renin-aldosterone axis and blood pressure in black children.
J. Pediatr.
PUBLISHED: 03-19-2013
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To comparatively examine the effects of adiposity on the levels of plasma renin activity (PRA), plasma aldosterone concentration (PAC), and aldosterone-renin ratio (ARR) in young black and white children.
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Spatial-spectral method for classification of hyperspectral images.
Opt Lett
PUBLISHED: 03-19-2013
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Spatial-spectral approach with spatially adaptive classification of hyperspectral images is proposed. The rotation-invariant spatial texture information for each object is exploited and incorporated into the classifier by using the modified local Gabor binary pattern to distinguish different types of classes of interest. The proposed method can effectively suppress anisotropic texture in spatially separate classes as well as improve the discrimination among classes. Moreover, it becomes more robust with the within-class variation. Experimental results on the classification of three real hyperspectral remote sensing images demonstrate the effectiveness of the proposed approach.
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Nonadherence to clinic appointments among HIV-infected children in an ambulatory care program in western Kenya.
J. Acquir. Immune Defic. Syndr.
PUBLISHED: 03-08-2013
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Nonadherence to clinic appointments is associated with poor outcomes in HIV-infected adults. We describe the effect of cumulative clinic adherence (CCA) to clinic appointments on mortality and loss to follow-up (LTFU) among HIV-infected children in Kenya.
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Blind Poissonian images deconvolution with framelet regularization.
Opt Lett
PUBLISHED: 03-05-2013
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We propose a maximum a posteriori blind Poissonian images deconvolution approach with framelet regularization for the image and total variation (TV) regularization for the point spread function. Compared with the TV based methods, our algorithm not only suppresses noise effectively but also recovers edges and detailed information. Moreover, the split Bregman method is exploited to solve the resulting minimization problem. Comparative results on both simulated and real images are reported.
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Dual differentiation-exogenous mesenchymal stem cell therapy for traumatic spinal cord injury repair in a murine hemisection model.
Stem Cells Int
PUBLISHED: 02-20-2013
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Mesenchymal stem cell (MSC) transplantation has shown tremendous promise as a therapy for repair of various tissues of the musculoskeletal, vascular, and central nervous systems. Based on this success, recent research in this field has focused on complex tissue damage, such as that which occurs from traumatic spinal cord injury (TSCI). As the critical event for successful exogenous, MSC therapy is their migration to the injury site, which allows for their anti-inflammatory and morphogenic effects on fracture healing, neuronal regeneration, and functional recover. Thus, there is a need for a cost-effective in vivo model that can faithfully recapitulate the salient features of the injury, therapy, and recovery. To address this, we review the recent advances in exogenous MSC therapy for TSCI and traumatic vertebral fracture repair and the existing challenges regarding their translational applications. We also describe a novel murine model designed to take advantage of multidisciplinary collaborations between musculoskeletal and neuroscience researchers, which is needed to establish an efficacious MSC therapy for TSCI.
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Expression of MSP58 in hepatocellular carcinoma.
Med. Oncol.
PUBLISHED: 02-09-2013
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We have investigated the expression and role of the 58-kDa micro-spherule protein (MSP58) in hepatocellular carcinoma (HCC). Immunohistochemistry was performed in 252 samples from patients with HCC to detect the expression level of MSP58. Results indicated that the expression level of MSP58 in the cancer samples was significantly higher than that in adjacent normal tissues. The Wilcoxon-Mann-Whitney test showed significant difference in the expression of MSP58 in patients with serum AFP, tumor size, histological differentiation, and universal integrated circuit card (UICC) stage (P < 0.001, P = 0.004, P < 0.001, P < 0.001, respectively). A total of 252 HCC patients were followed up for five consecutive years, and Kaplan-Meier survival analysis demonstrated that the survival time of HCC patients with low expression of MSP58 was longer than those with high expression during the 5-year follow-up period (P < 0.001). Cox regression analysis indicated that high expression of MSP58 (++ or +++), serum AFP (?25 ?g/L), tumor size (?3 cm), and UICC stage (III or IV) were the independent poor prognostic factors of HCC (P = 0.008, 0.0290, 0.001, 0.047, respectively). Furthermore, down-regulation of MSP58 was introduced to HCC cell lines (HepG2 and Huh7) by plasmid transfection. In vivo and in vitro studies indicated that MSP58si markedly reduced proliferation and promoted the apoptosis of HepG2 and Huh7 cells. In summary, our results demonstrated that MSP58 played an important role in the proliferation and apoptosis of HCC cells and the expression of MSP58 in HCC patients was closely related to the prognosis.
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Anatomic study of selective neurectomy of gastrocnemius muscle for calf reduction in Chinese.
J Plast Reconstr Aesthet Surg
PUBLISHED: 01-28-2013
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Selective neurectomy of the innervating nerves of the gastrocnemius muscle is a popular method employed for calf reduction. However, accidental injury to the untargeted adjacent nerves could happen. This study aims to provide detailed morphometry of the motor branches from the tibial nerve innervating the gastrocnemius muscle, the soleus and the medial sural cutaneous nerve in the popliteal fossa.
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Frequency and factors associated with adherence to and completion of combination antiretroviral therapy for prevention of mother to child transmission in western Kenya.
J Int AIDS Soc
PUBLISHED: 01-23-2013
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The objective of this analysis was to identify points of disruption within the prevention of mother-to-child transmission (PMTCT) continuum from combination antiretroviral therapy (CART) initiation until delivery.
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Content determination of the major constituents of Yinchenzhufu decoction via ultra high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry.
J Pharm Biomed Anal
PUBLISHED: 01-10-2013
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In this study, we developed a method using ultra high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry for determining the contents of chlorogenic acid, atractylenolide I, atractylenolide III, benzoylaconine, benzoylmesaconine, benzoylhypaconine, glycyrrhizic acid, glycyrrhetic acid, liquiritigenin, and cinnamic acid in Yinchenzhufu decoction, a classic traditional Chinese medicine prescription. Separation was performed on a C18 column (4.6mm i.d.×250mm, 5?m) and achieved with good linearity (r(2)>0.9984) within 35min. Gradient elution was applied using a mobile phase of 0.05% acetic acid/acetonitrile. The analytes were quantified on an LCQ ion trap mass spectrometer in electrospray ionisation full-scan mode. Variations in the intra- and inter-day precision of all analytes were below 4.57%, and the accuracy was evaluated by a recovery test within the range of 97.88-102.25%. The method successfully quantified the 10 compounds in five sample batches of Yinchenzhufu decoction, and the results show that the method is accurate, sensitive, and reliable.
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Expression of NDRG2 in human lung cancer and its correlation with prognosis.
Med. Oncol.
PUBLISHED: 01-10-2013
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We had reported that N-myc downstream-regulated gene (NDRG2) regulates colorectal cancer, breast cancer, clear cell renal cell carcinoma, pancreatic cancer, thyroid cancer and esophageal squamous cell proliferation, development, and apoptosis. The goal of this study was to determine the expression pattern of NDRG2 in human lung cancer and its correlation with prognosis. Immunohistochemistry, RT-PCR and western blot were used to explore the expression of NDRG2 in 185 human lung cancer patients. The correlation of NDRG2 expression with patients survival rate was assessed by Kaplan-Meier and Cox regression. Results showed that the expression level of NDRG2 was decreased in human lung cancer tissues, and NDRG2 was positively correlated with depth of invasion (P = 0.038), vascular invasion (P = 0.036), tumor grade (P = 0.039), and tumor size (P = 0.026). Both RT-PCR and Western blots demonstrated that NDRG2 mRNA and protein levels were lower in lung cancer compared to the adjacent normal tissue from the same individual, and NDRG2 level was negatively correlated with UICC stage. Additionally, survival time of lung cancer patients with high expression of NDRG2 was longer than those with low expression during the 5-year follow-up period (P = 0.001). Meanwhile, COX regression analysis indicated that low expression of NDRG2, ?pT(3), pM(1), ?pN(1) and vascular invasion were independent, poor prognostic factors of lung cancer patients. These data showed that NDRG2 may play an important role in human lung cancer tumourigenesis, and NDRG2 might serve as a novel prognostic marker in human lung cancer.
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Simultaneous determination of eleven major components in Fugan Fang using high-performance liquid chromatography coupled with mass spectrometry.
Biomed. Chromatogr.
PUBLISHED: 01-06-2013
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Fugan fang (FGF) is a traditional Chinese medicine (TCM) prescription that has been used in treating hepatic illnesses for many years. In this study, an analytical method is developed for the quantitative analysis of the major components of FGF. This method is based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS) on a reverse-phase C18 column. Results show that 0.01% acetic acid and acetonitrile is the optimum mobile phase in gradient elution. All compounds showed good linearity (r(2) ? 0.9948). Recoveries measured at three concentration levels varied from 83.5 to 104.8%. The method was validated with respect to precision, repeatability and accuracy and was successfully applied in the quantification of the 11 components of FGF products. The validated HPLC-MS method provides a basis for assessing the quality of TCM prescriptions containing many bioactive components.
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Rapid and efficient ultrasound-assisted method for the combinatorial synthesis of spiro[indoline-3,4-pyrano[2,3-c]pyrazole] derivatives.
ACS Comb Sci
PUBLISHED: 12-19-2011
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An efficient one-pot synthesis of spiro[indoline-3,4-pyrano[2,3-c]pyrazole] derivatives by four-component reaction of hydrazine, ?-keto ester, isatin, and malononitrile or ethyl cyanoacetate catalyzed by piperidine under ultrasound irradiation is described. This synthesis was confirmed to follow the group-assistant-purification chemistry (GAP) chemistry process, which can avoid traditional chromatography and recrystallization purifications.
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Improving the balance of carrier mobilities of host-guest solid-state light-emitting electrochemical cells.
Phys Chem Chem Phys
PUBLISHED: 12-01-2011
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We report efficient host-guest solid-state light-emitting electrochemical cells (LECs) utilizing a cationic terfluorene derivative as the host and a red-emitting cationic transition metal complex as the guest. Carrier trapping induced by the energy offset in the lowest unoccupied molecular orbital (LUMO) levels between the host and the guest impedes electron transport in the host-guest films and thus improves the balance of carrier mobilities of the host films intrinsically exhibiting electron preferred transporting characteristics. Photoluminescence measurements show efficient energy transfer in this host-guest system and thus ensure predominant guest emission at low guest concentrations, rendering significantly reduced self-quenching of guest molecules. EL measurements show that the peak EQE (power efficiency) of the host-guest LECs reaches 3.62% (7.36 lm W(-1)), which approaches the upper limit that one would expect from the photoluminescence quantum yield of the emissive layer (?0.2) and an optical out-coupling efficiency of ?20% and consequently indicates superior balance of carrier mobilities in such a host-guest emissive layer. These results are among the highest reported for red-emitting LECs and thus confirm that in addition to reducing self-quenching of guest molecules, the strategy of utilizing a carrier transporting host doped with a proper carrier trapping guest would improve balance of carrier mobilities in the host-guest emissive layer, offering an effective approach for optimizing device efficiencies of LECs.
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Phenolic compounds from the rhizomes of Dioscorea bulbifera.
Chem. Biodivers.
PUBLISHED: 11-16-2011
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One new bibenzyl, 7, and one new diarylheptanone, diobulbinone A (18), together with sixteen known compounds, 1-6 and 8-17, have been isolated form the rhizomes of Dioscorea bulbifera. Their structures were elucidated by NMR and MS analyses. Compound 7 showed high antioxidant capacity in FRAP assay and DPPH radical-scavenging activity.
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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.