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Find video protocols related to scientific articles indexed in Pubmed.
CTCF controls HOXA cluster silencing and mediates PRC2-repressive higher-order chromatin structure in NT2/D1 cells.
Mol. Cell. Biol.
PUBLISHED: 08-18-2014
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HOX cluster genes are activated sequentially in their positional order along the chromosome during vertebrate development. This phenomenon, known as temporal colinearity, depends on transcriptional silencing of 5' HOX genes. Chromatin looping was recently identified as a conserved feature of silent HOX clusters, with CCCTC-binding factor (CTCF) binding sites located at the loop bases. However, the potential contribution of CTCF to HOX cluster silencing and the underlying mechanism have not been established. Here, we demonstrate that the HOXA locus is organized by CTCF into chromatin loops and that CTCF depletion causes significantly enhanced activation of HOXA3 to -A7, -A9 to -A11, and -A13 in response to retinoic acid, with the highest effect observed for HOXA9. Our subsequent analyses revealed that CTCF facilitates the stabilization of Polycomb repressive complex 2 (PRC2) and trimethylated lysine 27 of histone H3 (H3K27me3) at the human HOXA locus. Our results reveal that CTCF functions as a controller of HOXA cluster silencing and mediates PRC2-repressive higher-order chromatin structure.
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Literature review on clinical decision support system reducing medical error.
J Evid Based Med
PUBLISHED: 08-08-2014
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Quite a number of studies on clinical decision support systems (CDSS) have been published in recent years to assess the characteristics and architecture of CDSS and evaluate the effects of CDSS on clinical work. However, until now there have been no relevant studies to investigate the quantity of these, and their contribution to present day thinking. The aim of this study was to explore the areas of theme, and the study design of research on CDSS in literature published in English and Chinese-language journals.
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Readministration of EGFR tyrosine kinase inhibitor in non-small cell lung cancer patients after initial failure, what affects its efficacy?
Sci Rep
PUBLISHED: 08-08-2014
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Few therapeutic options are available for non-small cell lung cancer (NSCLC) after failure to primary epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs). Since TKI rechallenge is one of the most common salvage approaches in clinical practice, we sought to identify the independent factors that associated with 2(nd) progression progression-free survival (PFS) and overall survival (OS). Seventy-two consecutive EGFR-mutated NSCLC patients with TKI retreatment after initial failure were retrospectively analyzed in this study. Univariate survival analysis and Cox proportional hazards regression model was used to determine if EGFR-TKIs readministration is tolerable as well as efficacious for a certain group of patients.
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[Protective effect of alkaloids from Piper longum in rat dopaminergic neuron injury of 6-OHDA-induced Parkinson's disease].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 08-07-2014
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To discuss the protective effect of alkaloids from Piper longum (PLA) in rat dopaminergic neuron injury of 6-OHDA-induced Parkinson's disease and its possible mechanism.
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Lentivirus vectors construction of SiRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7.
Asian Pac J Trop Med
PUBLISHED: 07-10-2014
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To build GPC3 gene short hairpin interference RNA (shRNA) slow virus vector, observe expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liver cancer cell growth, and provide theoretical basis for gene therapy of liver cancer.
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Single-crystal X-ray diffraction studies on structural transformations of porous coordination polymers.
Chem Soc Rev
PUBLISHED: 06-28-2014
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X-Ray single-crystal diffraction has been the most straightforward and important technique in structural determination of crystalline materials for understanding their structure-property relationships. This powerful tool can be used to directly visualize the precise and detailed structural information of porous coordination polymers or metal-organic frameworks at different states, which are unique for their flexible host frameworks compared with conventional adsorbents. With a series of selected recent examples, this review gives a brief overview of single-crystal X-ray diffraction studies and single-crystal to single-crystal transformations of porous coordination polymers under various chemical and physical stimuli such as solvent and gas sorption/desorption/exchange, chemical reaction and temperature change.
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CsIFIT1, an interferon-induced protein with tetratricopeptide repeat, inhibits viral infection in tongue sole (Cynoglossus semilaevis).
Fish Shellfish Immunol.
PUBLISHED: 06-17-2014
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Interferon-induced protein with tetratricopeptide repeats (IFIT) is involved in antiviral immunity in mammalian cells. In fish, the function of this group of proteins is unknown. In this study, we report the identification of an IFIT homologue (named CsIFIT1) from tongue sole (Cynoglossus semilaevis) and examined its antiviral potential. CsIFIT1 is composed of 436 residues and shares 30.9%-58.7% overall sequence identities with the known IFITs of a number of teleost species. In silico analysis identified two tetratricopeptide repeats in CsIFIT1. Quantitative real time RT-PCR analysis showed that CsIFIT1 expression was most abundant in immune relevant organs and upregulated, in a time-dependent manner, by experimental infection with the fish viral pathogen megalocytivirus. When CsIFIT1 was overexpressed in tongue sole before megalocytivirus infection, viral replication in fish tissues was blocked to significant extents. Consistently, when CsIFIT1 expression in tongue sole was knocked down by siRNA, viral replication in fish tissues was significantly enhanced. Taken together, these results indicate that CsIFIT1 is likely a key factor of antiviral immunity and is required for optimal defense against viral infection.
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A complex mechanism for HDGF-mediated cell growth, migration, invasion, and TMZ chemosensitivity in glioma.
J. Neurooncol.
PUBLISHED: 06-17-2014
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HDGF is overexpressed in gliomas as compared to normal brain. We therefore analyzed the molecular mechanisms of HDGF action in gliomas. HDGF was downregulated in normal brain tissue as compared to glioma specimens at both the mRNA and the protein levels. In glioma samples, increased HDGF expression was associated with disease progression. Knocking down HDGF expression not only significantly decreased cellular proliferation, migration, invasion, and tumorigenesis, but also markedly enhanced TMZ-induced cytotoxicity and apoptosis in glioma cells. Mechanistic analyses revealed that CCND1, c-myc, and TGF-? were downregulated after stable HDGF knockdown in the U251 and U87 glioma cells. HDGF knockdown restored E-cadherin expression and suppressed mesenchymal cell markers such as vimentin, ?-catenin, and N-cadherin. The expression of cleaved caspase-3 increased, while Bcl-2 decreased in each cell line following treatment with shHDGF and TMZ, as compared to TMZ alone. Furthermore, RNAi-based knockdown study revealed that HDGF is probably involved in the activation of both the PI3K/Akt and the TGF-? signaling pathways. Together, our data suggested that HDGF regulates glioma cell growth, apoptosis and epithelial-mesenchymal transition (EMT) probably through the Akt and the TGF-? signaling pathways. These results provide evidence that targeting HDGF or its downstream targets may lead to novel therapies for gliomas.
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SIRT1-mediated epigenetic downregulation of plasminogen activator inhibitor-1 prevents vascular endothelial replicative senescence.
Aging Cell
PUBLISHED: 06-15-2014
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The inactivation of plasminogen activator inhibitor-1 (PAI-1) has been shown to exert beneficial effects in age-related vascular diseases. Limited information is available on the molecular mechanisms regarding the negatively regulated expression of PAI-1 in the vascular system. In this study, we observed an inverse correlation between SIRT1, a class III histone deacetylase, and PAI-1 expression in human atherosclerotic plaques and the aortas of old mice, suggesting that internal negative regulation exists between SIRT1 and PAI-1. SIRT1 overexpression reversed the increased PAI-1 expression in senescent human umbilical vein endothelial cells (HUVECs) and aortas of old mice, accompanied by decreased SA-?-gal activity in vitro and improved endothelial function and reduced arterial stiffness in vivo. Moreover, the SIRT1-mediated inhibition of PAI-1 expression exerted an antisenescence effect in HUVECs. Furthermore, we demonstrated that SIRT1 is able to bind to the PAI-1 promoter, resulting in a decrease in the acetylation of histone H4 lysine 16 (H4K16) on the PAI-1 promoter region. Thus, our findings suggest that the SIRT1-mediated epigenetic inhibition of PAI-1 expression exerts a protective effect in vascular endothelial senescence.
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Enhanced electroluminescence using Ta?O?/ZnO/HfO? asymmetric double heterostructure in ZnO/GaN-based light emitting diodes.
Opt Express
PUBLISHED: 06-13-2014
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ZnO/GaN-based light-emitting diodes (LEDs) with improved asymmetric double heterostructure of Ta?O?/ZnO/HfO? have been fabricated. Electroluminescence (EL) performance has been enhanced by the HfO? electron blocking layer and further improved by continuing inserting the Ta?O? hole blocking layer. The origins of the emission have been identified, which indicated that the Ta?O?/ZnO/HfO? asymmetric structure could more effectively confine carriers in the active i-ZnO layer and meanwhile suppresses of radiation from GaN. This device exhibits superior stability in long-time running. It's hoped that the asymmetric double heterostructure may be helpful for the development of the future ZnO-based LEDs.
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Au@MnO2 Core-Shell Nanomesh Electrodes for Transparent Flexible Supercapacitors.
Small
PUBLISHED: 05-07-2014
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A novel Au@MnO2 supercapacitor is presented. The sophisticated core-shell architecture combining an Au nanomesh core with a MnO2 shell on a flexible polymeric substrate is demonstrated as an electrode for high performance transparent flexible supercapacitors (TFSCs). Due to their unique structure, high areal/gravimetric capacitance and rate capability for TFSCs are achieved.
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Unusual electroluminescence from n-ZnO@i-MgO core-shell nanowire color-tunable light-emitting diode at reverse bias.
Phys Chem Chem Phys
PUBLISHED: 04-10-2014
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Light-emitting diodes (LEDs) based on n-ZnO@i-MgO core-shell (CS) nanowires (NWs) are herein demonstrated and characterized. MgO insulating layers were rationally introduced as shells to modify/passivate the surface defects of ZnO NWs. A high-quality ZnO/MgO interface was attained and the optically pumped near-band-edge emission of the bare ZnO NWs was greatly enhanced after cladding i-MgO shells. Electroluminescence (EL) spectra measured in the whole UV-visible range revealed that light emission can only be detected when LEDs were applied with reverse bias. Moreover, the emission color can be tuned from orange to bright white with increasing reverse bias. We explored these interesting results tentatively in terms of the energy-band diagram of the heterojunction and it was found that the interfacial i-MgO shells not only acted as an insulator to prevent a short circuit between the two electrodes, but also offered a potential energy difference so that electron tunneling was energetically possible, both of which were essential to generate the reverse-bias EL. The dipole-forbidden d-d transitions by the Laporte selection rule in the p-NiO might be the reason to why there is no light being detected from the CS NW LED under forward bias. It is hoped that this simple and facile route may provide an effective approach in designing low-cost CS NW LEDs.
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(2-Chloro-pyrimidin-4-yl)ferrocene.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 04-01-2014
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In the title compound, [Fe(C5H5)(C9H6ClN2)], the two cyclo-penta-dienyl rings are almost parallel, subtending a dihedral angle of 3.01?(5)°. The dihedral angle between the substituted cyclo-penta-dienyl ring and the pyrimidinyl ring is 12.02?(1)°. The conformation of the two cyclopentadienyl rings in the ferrocenyl moiety is eclipsed.
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Prognostic factors in patients with recurrence after complete resection of esophageal squamous cell carcinoma.
J Thorac Dis
PUBLISHED: 03-24-2014
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Recurrence following complete resection of esophageal squamous cell carcinoma (SCC) still remains common. The aim of this study was to investigate the prognostic factors in patients with recurrence after complete resection of esophageal SCC.
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Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in glioma.
Mol. Cancer
PUBLISHED: 03-13-2014
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The success of using glycolytic inhibitors for cancer treatment relies on better understanding the roles of each frequently deregulated glycolytic genes in cancer. This report analyzed the involvement of a key glycolytic enzyme, alpha-enolase (ENO1), in tumor progression and prognosis of human glioma.
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Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02.
Braz. J. Microbiol.
PUBLISHED: 03-10-2014
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The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.
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Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.
J Proteomics
PUBLISHED: 03-01-2014
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Olfactory sensory information is processed and integrated by circuits within the olfactory bulb (OB) before being sent to the olfactory cortex. In the mammalian OB, neural activity driven by external stimuli can lead to experience-dependent changes in structures and functions. In this study, quantitative proteomics techniques were employed to study proteome-wide changes in the OB under four levels of neural activity (from low to high): devoid of peripheral input (using a transgenic model), wild-type control, and short-term and long-term odor exposures. Our results revealed that proteins related to various processes were altered in the OBs of odor-deprived and odor-stimulated mice compared to the wild-type controls. These changes induced by odor stimulation were quite different from those induced by a deficit in peripheral olfactory inputs. Detailed analysis demonstrated that metabolic process and synaptic transmission were the most commonly altered pathways and that the effects of peripheral deprivation were more profound. Our comparative proteomics analysis indicated that olfactory deprivation and odor exposure lead to different alterations in the OB proteome, which provides new clues about the mechanisms underlying the olfactory deprivation- or odor stimulation-induced plasticity of OB function and organization.
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Graphenal polymers for energy storage.
Small
PUBLISHED: 02-27-2014
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A key to improve the electrochemical performance of energy storage systems (e.g., lithium ion batteries and supercapacitors) is to develop advanced electrode materials. In the last few years, although originating from the unique structure and property of graphene, interest has expanded beyond the originally literally defined graphene into versatile integration of numerous intermediate structures lying between graphene and organic polymer, particularly for the development of new electrode materials for energy storage devices. Notably, diverse designations have shaded common characteristics of the molecular configurations of these newly-emerging materials, severely impeding the design, synthesis, tailoring, functionalization, and control of functional electrode materials in a rational and systematical manner. This concept paper highlights all these intermediate materials, specifically comprising graphene subunits intrinsically interconnected by organic linkers or fractions, following a general concept of graphenal polymers. Combined with recent advances made by our group and others, two representative synthesis approaches (bottom-up and top-down) for graphenal polymers are outlined, as well as the structure-property relationships of these graphenal polymers as energy storage electrode materials are discussed.
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Production and in vivo antioxidant activity of Zn, Ge, Se-enriched mycelia by Cordyceps sinensis SU-01.
Curr. Microbiol.
PUBLISHED: 02-26-2014
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Cordyceps sinensis, a traditionally edible and medicinal fungus in China, cannot be artificially solid-cultured. Zinc (Zn), germanium (Ge), and selenium (Se) are the essential trace elements for human body. In this work, C. sinensis SU-01 was cultivated in liquid medium simultaneously containing Zn, Ge, and Se. The bioactive ingredients and in vivo antioxidant activities of Zn, Ge, Se-enriched mycelia (ZGSM) of C. sinensis SU-01 were investigated. Under the determined conditions, the Zn, Ge, and Se contents of ZGSM were 2543.16 ± 158.92, 1873.85 ± 81.82, and 1260.16 ± 107.12 ?g/g, respectively. The optimal concentrations of Zn, Ge, and Se had a positive effect on biosynthesis of protein, polysaccharide, cordycepic acid, and amino acids. The activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) of mice blood were 3.72 ± 0.15 and 28.74 ± 2.53 % higher than that of control, respectively, and the content of malondialdehyde (MDA) was 41.01 ± 3.66 % lower than that of control.
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Hand-assisted thoracoscopic surgery for pulmonary metastasectomy through sternocostal triangle access: superiority in detection of non-imaged pulmonary nodules.
Sci Rep
PUBLISHED: 01-29-2014
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Hand-Assisted Thoracoscopic Surgery for pulmonary metastasectomy through sternocostal triangle access allows manual palpation of both lungs, thus permitting effective treatment of lung metastases. In our research, 62 patients from November 2001 to January 2012 underwent our Hand-Assisted Thoracoscopic Surgery procedures for pulmonary metastasectomy. Clinical data, including the number of pulmonary metastases determined by Computed Tomography/Positron Emission Tomography-Computed Tomography, surgical findings and survival data of these patients were collected. We found that the median follow-up time was 23.7 months (range 2.4 to 85.6 months). 30 cases of them had post-operative recurrences and the median disease-free survival period was 27.4 months. For Computed Tomography scan, the overall sensitivity for proved metastases was 63% (115/182). 67 non-imaged malignant nodules were palpated and removed in 14 cases. For Positron Emission Tomography-Computed Tomography scan, the overall sensitivity was 66% (79/120). 41 non-imaged malignant nodules were palpated and removed in 12 cases. This study show that the Hand-Assisted Thoracoscopic Surgery provides an easier way for routine bilateral pleural exploration, and thus is critical and effective in detection of non-imaged malignant pulmonary metastases, which might contribute to long-term disease-free survival.
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Topographic variations of the optic chiasm and the pituitary stalk: a morphometric study based on midsagittal T2-weighted MR images.
Surg Radiol Anat
PUBLISHED: 01-28-2014
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This study was conducted to characterize topographic variations of the optic chiasm (OC) and the pituitary stalk (PS) in situ based on MR images.
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A new flavanone from the aerial parts of Penthorum chinense.
Nat. Prod. Res.
PUBLISHED: 01-23-2014
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A new flavanone, 5-methoxy-pinocembrin-7-O-?-D-glucoside (1), together with six known compounds, quercetin-3-O-?-D-xyloside (2), quercetin-3'-O-?-L-rhamnoside (3), quercetin (4), pinocembrin-7-O-?-D-glucoside (5), pinocembrin (6), gallic acid (7), were isolated and identified from the aerial parts of Penthorum chinense. Compounds 2 and 3 were isolated from this plant for the first time.
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Antibacterial and antiviral properties of tongue sole (Cynoglossus semilaevis) high mobility group B2 protein are largely independent on the acidic C-terminal domain.
Fish Shellfish Immunol.
PUBLISHED: 01-16-2014
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High mobility group box (HMGB) proteins are known to be involved in diverse functions in mammalian cells. In teleost, very limited studies on HMGB proteins have been documented. In this study, we reported identification of a HMGB homologue (named CsHMGB2) from tongue sole (Cynoglossus semilaevis) and examined its biological property. CsHMGB2 is 245 residues in length and contains two basic HMG boxes and an acidic C-terminal tail composed of 23 Asp/Glu residues. Quantitative real time RT-PCR (qRT-PCR) analysis showed that CsHMGB2 expression occurred in multiple tissues and was upregulated by bacterial and viral infection in a time-dependent manner. In vitro studies showed that when tongue sole peripheral blood leukocytes were treated with recombinant CsHMGB2 (rCsHMGB2) and the mutant rCsHMGB2M, which bears a deletion of the C-terminal acidic region, significant and comparable increases in cellular resistance against bacterial infection were observed. qRT-PCR detected enhanced expression of proinflammatory cytokines and chemokines in rCsHMGB2-treated cells. In vivo studies showed that when tongues sole were administered with rCsHMGB2 or rCsHMGB2M before being subjected to bacterial and viral infection, the pathogen loads in the spleen and kidney of the fish were significantly reduced. Taken together, these results suggest that CsHMGB2 possesses immunoregulatory properties that promote resistance against bacterial and viral infection in a manner that is largely independent on the highly conserved C-terminal acidic domain.
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Above-room-temperature ferroelastic phase transition in a perovskite-like compound [N(CH3)4][Cd(N3)3].
Chem. Commun. (Camb.)
PUBLISHED: 01-14-2014
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A new perovskite-like compound [N(CH3)4][Cd(N3)3] is reported here, which undergoes a series of reversible phase transitions including an above-room-temperature ferroelastic phase transition. An order-disorder mechanism is found in these structural transitions owing to the sway of the rod-like N3(-) bridges as well as the rotation of the tetrahedron-like [N(CH3)4](+) guests.
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Histomorphochemical comparison of microfracture as a first-line and a salvage procedure: is microfracture still a viable option for knee cartilage repair in a salvage situation?
J. Orthop. Res.
PUBLISHED: 01-13-2014
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Microfracture is considered as the first-line procedure for knee cartilage repair, but the results of microfracture seem less predictable and rather controversial in a salvage situation. Thus, the purpose of the study was to histomorphochemically compare microfracture as a salvage procedure with microfracture as a first-line procedure in a rabbit model. We hypothesized that microfracture in a salvage situation would result in histomorphochemically inferior cartilage repair compared to microfracture as a first-line procedure, and the inferiority would be attributed to less migration of reparable marrow cells to the defect due to destruction of microarchitecture of the subchondral bone. Thirty-six New Zealand white rabbits were divided into three groups: (i) untreated full-thickness chondral defect, (ii) single microfracture treatment (first microfracture group), and (iii) repeated microfracture in 8 weeks after the first procedure (second microfracture group). In each group, rabbits were sacrificed at the end of 8 weeks, and osteochondral specimens at the repair sites were obtained for histomorphochemical analysis. Results showed that microfracture as a salvage procedure resulted in overall inferior cartilage repair histomorphochemically compared with microfracture as a first-line procedure, which correlated with deteriorative changes in the quality of underlying subchondral bone rather than intrinsic incapability to recruit the reparative cells in the defect area. In conclusion, although a comparable number of reparable cells and a mechanically weakened subchondral bone are anticipated, more study is necessary to clearly determine when a microfracture should be performed in a situation.
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Synthesis and field emission studies of tower-like GaN nanowires.
Nanoscale Res Lett
PUBLISHED: 01-01-2014
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Tower-like GaN nanowires were successfully fabricated on Au-coated Si substrates by chemical vapor deposition. The tower-like nanowire consisted of a nanowire at the center and microcrystal layers stacked one by one around the nanowire. The tower-like nanowires grew along the [0001] direction, and the exposed surfaces of the microcrystal layers are [Formula: see text] and [Formula: see text] facets. The growth mechanism of the tower-like GaN nanowires was proposed. The field emission property of tower-like GaN nanowires was tested. Due to the sharp tips, nearly vertical alignment and rough surfaces caused by the microcrystal layers, the tower-like GaN nanowires show excellent performance in field emission with a turn-on field of 2.44 V/?m which is lower than those of other GaN one-dimensional (1D) nanomaterials.
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[Expression of thymidylate synthase and dihydropyrimidine dehydrogenase in penile cancer].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 12-12-2013
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To investigate the expression of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) in penile cancer.
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VATS lobectomy facilitates the delivery of adjuvant docetaxel-carboplatin chemotherapy in patients with non-small cell lung cancer.
J Thorac Dis
PUBLISHED: 11-21-2013
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To evaluate the safety and tolerability of docetaxel/carboplatin regimen in the post-operative setting of patients with non-small cell lung cancer (NSCLC).
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PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1.
Blood
PUBLISHED: 11-19-2013
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Promyelocytic leukemia protein (PML) has been implicated as a participant in multiple cellular processes including senescence, apoptosis, proliferation and differentiation. Studies of PML function in hematopoietic differentiation have previously focused principally on its myeloid activities, and also indicated that PML is involved in erythroid colony formation. However, the exact role that PML plays in erythropoiesis is essentially unknown. In this report, we found that PML4, a specific PML isoform expressed in erythroid cells, promotes endogenous erythroid genes expression in K562 as well as in primary human erythroid cells. We show that the PML4 effect is GATA-1-dependent using GATA-1 knockout/rescued G1E/G1E-ER4 cells. PML4, but not other detected PML isoforms, directly interact with GATA-1 and can recruit it into PML nuclear bodies. Furthermore, PML4 facilitates GATA-1 trans-activation activity in an interaction-dependent manner. Finally, we present evidence that PML4 enhances GATA-1 occupancy within the globin gene cluster and stimulates cooperation between GATA-1 and its co-activator, p300. These results demonstrate that PML4 is an important regulator of GATA-1 and participates in erythroid differention by enhancing GATA-1 trans-activation activity.
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[Clinical research on children mental retardation treated with acupuncture].
Zhongguo Zhen Jiu
PUBLISHED: 11-08-2013
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To observe the impacts of acupuncture on intelligent structure, social adaptability and fMRI brain function in children mental retardation (MR).
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Mutation abundance affects the efficacy of EGFR tyrosine kinase inhibitor readministration in non-small-cell lung cancer with acquired resistance.
Med. Oncol.
PUBLISHED: 11-03-2013
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There is no consensus in the salvage treatment for non-small-cell lung cancer (NSCLC) with acquired resistance to primary epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs). Fifty-one consecutive EGFR-mutated NSCLC patients with TKI retreatment after acquired resistance were enrolled in this study. The quantitation of mutation abundance was performed by real-time fluorescent quantitative PCR. The correlation between mutation abundance and outcomes of readministrated TKI was analyzed by survival analysis. Patients with high (H) mutation abundance (24/51) had a significantly (log-rank, P < 0.05) longer (5.27-2.53 months) median progression-free survival (PFS), compared with the low (L) abundance group (27/51), whereas the median overall survival showed no difference (21.00-18.20 months, log-rank P = .403) between the two groups. Objective response and disease control rates in group H and group L regarding the second round TKI treatment were 8.3, 70.8 and 0, 48.1 %, respectively. Groupings with different mutation abundances were significantly associated with PFS under multivariate Cox proportional hazards regression model [hazard ratio (HR) for group H vs. L, 0.527; P = .036]. Mutation abundance affects the efficacy of EGFR-TKIs readministration in NSCLC with acquired resistance. The quantitative mutation abundance of EGFR may be a potential predictor for selecting optimal patients to readministrate EGFR-TKIs after acquired resistance to primary TKI.
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Marrow stromal stem cell autologous transplantation in denervated fracture healing: an experimental study in rats.
Orthop Surg
PUBLISHED: 10-25-2013
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To investigate the influence of bone marrow stromal stem cell (BMSCs) transplantation on healing of fractures combined with central nerve injuries in rats.
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A flexible porous Cu(ii) bis-imidazolate framework with ultrahigh concentration of active sites for efficient and recyclable CO2 capture.
Chem. Commun. (Camb.)
PUBLISHED: 10-18-2013
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By virtue of planar Cu2(?-OH)2(2+) cluster units, a flexible porous metal azolate framework not only adsorbs large amounts of CO2 (19.6 wt% or 266 g L(-1) at 298 K and 1 atm) with a high zero-coverage adsorption enthalpy (-47 kJ mol(-1)), but also desorbs quickly at very low temperatures.
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Overexpression of SIRT1 in vascular smooth muscle cells attenuates angiotensin II-induced vascular remodeling and hypertension in mice.
J. Mol. Med.
PUBLISHED: 07-19-2013
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Angiotensin II (AngII) induces the development of vascular hypertrophy and hypertension. We have shown previously that overexpression of class III deacetylase SIRT1 inhibits AngII-induced hypertrophy in vascular smooth muscle cells (VSMCs). However, the direct role of SIRT1 in VSMCs in response to AngII infusion in vivo remains unclear. Here, we found that the expression and activity of SIRT1 in mouse aortas was decreased significantly by AngII infusion. VSMC-specific SIRT1 transgene (SV-Tg) prevented the increase in systolic blood pressure (SBP) caused by AngII infusion without affecting heart function in mice. SIRT1 overexpression alleviated vascular remodeling in mouse thoracic and renal aortas induced by AngII infusion, and significantly inhibited reactive oxygen species (ROS) generation, vascular inflammation, and collagen synthesis in arterial walls. Reduced expression of transforming growth factor-? 1 (TGF-?1) was also observed in the aortas of AngII-infused SV-Tg mice. Moreover, SIRT1 overexpression decreased AngII-increased binding of nuclear factor-?B on its specific binding sites on TGF-?1 promoter. Taken together, these data demonstrate that SIRT1 overexpression in VSMCs reduces SBP and inhibits AngII-induced vascular remodeling in mice. The inhibition of vascular remodeling contributes, at least in part, to the antihypertensive effect of SIRT1.
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[The study of the protective effect and its mechanism of Edaravone to neurons with hydrogen peroxide stimulated].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 07-18-2013
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To prove the protective effect of Edaravone to neurons and to study the particular mechanism.
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Seedless synthesis of layered ZnO nanowall networks on Al substrate for white light electroluminescence.
Nanotechnology
PUBLISHED: 07-15-2013
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In this paper, layered ZnO nanowall networks were directly grown on Al substrates using a hydrothermal method without predepositing seed layers. The individual ZnO nanowalls with a thickness of several nanometers and a size of several hundred nanometers were (002) surface dominated, in which the preferential growth direction of ZnO was suppressed. White electroluminescence devices were fabricated based on Au/polymethylmethacrylate/ZnO-nanowall (metal-insulator-semiconductor) structures. The chromaticity coordinate of the electroluminescence spectrum for the optimal device was calculated as (0.27, 0.34), which is close to (0.33, 0.33) of standard white light.
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Clinical characteristics of patients with solitary pulmonary mass after radical treatment for primary cancers: pulmonary metastasis or second primary lung cancer?
Cancer Invest.
PUBLISHED: 06-11-2013
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We identified clinical characteristics of 30 pulmonary metastasis (PM) patients and 29 second primary lung cancer (SPLC) patients with feature of solitary pulmonary mass (SPM) after radical treatment of prior cancers. 6.7% and 44.8% patients presented with centrally located SPM and the median event-free durations were 33 and 72 months in PM and SPLC groups, respectively. PM was more likely to be found in prior cancers with stage III. In conclusion, the location of SPM, the event-free duration and the prior tumor staging were important features for differentiating SPLC from PM among patients with SPM after prior cancers.
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Carbonaceous electrode materials for supercapacitors.
Adv. Mater. Weinheim
PUBLISHED: 05-31-2013
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Supercapacitors have been widely studied around the world in recent years, due to their excellent power density and long cycle life. As the most frequently used electrode materials for supercapacitors, carbonaceous materials attract more and more attention. However, their relatively low energy density still holds back the widespread application. Up to now, various strategies have been developed to figure out this problem. This research news summarizes the recent advances in improving the supercapacitor performance of carbonaceous materials, including the incorporation of heteroatoms and the pore size effect (subnanopores contribution). In addition, a new class of carbonaceous materials, porous organic networks (PONs) has been managed into the supercapacitor field, which promises great potential in not only improving the supercapacitor performances, but also unraveling the related mechanisms.
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Hydrogen-induced effects on the CVD growth of high-quality graphene structures.
Nanoscale
PUBLISHED: 05-30-2013
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In this work, the hydrogen-induced effects on the CVD growth of high-quality graphene have been systematically studied by regulating the growth parameters mainly related to hydrogen. Experimental results demonstrate that under a high hydrogen flow rate, the competitive etching effect during the growth process is more prominent and even shows macroscopic selectivity. Based on these understandings, the hexagonal graphene domains with diverse edge modalities are controllably synthesized on a large scale by elaborately managing the competitive etching effect of hydrogen that existed during the formation of graphene. This study not only contributes to the understanding of the mechanism of CVD growth, especially the effects of hydrogen used in the system, but also provides a facile method to synthesize high-quality graphene structures with trimmed edge morphologies.
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Serum carbohydrate antigen 19-9 as an indicator of liver metastasis in colorectal carcinoma cases.
Asian Pac. J. Cancer Prev.
PUBLISHED: 04-30-2013
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The liver is the organ to which colorectal carcinomas (CRCs) most commonly metastasize, and surgical resection has been established as the most effective and potentially curative treatment for CRC with liver metastasis (LM). Therefore, surveillance of LM is vital for improvement of prognosis of CRC patients. In this study, we aimed to explore the potential value of carbohydrate antigen 19-9 (CA 19-9), carcinoembryonic antigen (CEA), and marker enzymes in indicating LM with CRC.
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Genome analysis and signature discovery for diving and sensory properties of the endangered Chinese alligator.
Cell Res.
PUBLISHED: 04-15-2013
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Crocodilians are diving reptiles that can hold their breath under water for long periods of time and are crepuscular animals with excellent sensory abilities. They comprise a sister lineage of birds and have no sex chromosome. Here we report the genome sequence of the endangered Chinese alligator (Alligator sinensis) and describe its unique features. The next-generation sequencing generated 314 Gb of raw sequence, yielding a genome size of 2.3 Gb. A total of 22 200 genes were predicted in Alligator sinensis using a de novo, homology- and RNA-based combined model. The genetic basis of long-diving behavior includes duplication of the bicarbonate-binding hemoglobin gene, co-functioning of routine phosphate-binding and special bicarbonate-binding oxygen transport, and positively selected energy metabolism, ammonium bicarbonate excretion and cardiac muscle contraction. Further, we elucidated the robust Alligator sinensis sensory system, including a significantly expanded olfactory receptor repertoire, rapidly evolving nerve-related cellular components and visual perception, and positive selection of the night vision-related opsin and sound detection-associated otopetrin. We also discovered a well-developed immune system with a considerable number of lineage-specific antigen-presentation genes for adaptive immunity as well as expansion of the tripartite motif-containing C-type lectin and butyrophilin genes for innate immunity and expression of antibacterial peptides. Multifluorescence in situ hybridization showed that alligator chromosome 3, which encodes DMRT1, exhibits significant synteny with chicken chromosome Z. Finally, population history analysis indicated population admixture 0.60-1.05 million years ago, when the Qinghai-Tibetan Plateau was uplifted.
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Loss of RASSF1A expression in colorectal cancer and its association with K-ras status.
Biomed Res Int
PUBLISHED: 04-06-2013
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The RAS-association domain family 1 A (RASSF1A) is a classical member of RAS effectors regulating cell proliferation and apoptosis. Loss of RASSF1A expression may shift the balance towards a growth-promoting effect without the necessity of activating K-ras mutations. Its potential association with K-ras mutations in colorectal cancer (CRC) is unclear.
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Effect of different bone marrow stimulation techniques (BSTs) on MSCs mobilization.
J. Orthop. Res.
PUBLISHED: 04-01-2013
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The therapeutic effect of bone marrow stimulation techniques (BSTs) is mainly attributed to the role of mesenchymal stem cells (MSCs) from the bone marrow. However, no studies have directly shown the amount of MSCs drained by BSTs. This study hypothesized that differences in the opening of subchondral bone affect the number of MSCs drained from the bone marrow. We purposed that as the exposed area and hole size of BSTs vary, the number of MSCs drained out was measured. Three groups of different BSTs were designed that have variations in the sizes of total exposed area and individual holes. Three different BSTs using a curette, 1.5- and 0.8-mm awls were carried out on the full-thickness femoral cartilage defect of young rabbits. After BST, the number of MSCs in the blood clot was measured by CFU-Fs assay. As the size of the total exposed area increased, so did the number of MSCs obtained. The number of MSCs drained from bone marrow may vary depending on different BSTs and this could affect therapeutic efficacy of cartilage defect. As current microfracture (MF) method cannot drain the most MSCs clinically, more improved surgery technique is needed.
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Experience with a novel retrograde wiring technique for coronary chronic total occlusion.
J Interv Cardiol
PUBLISHED: 03-31-2013
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We report our evaluation of a novel retrograde wiring technique known as the Rendezvous method.
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Inhibition of CK2? down-regulates Notch1 signalling in lung cancer cells.
J. Cell. Mol. Med.
PUBLISHED: 03-24-2013
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Protein kinase CK2 is frequently elevated in a variety of human cancers. The Notch1 signalling pathway has been implicated in stem cell maintenance and its aberrant activation has been shown in several types of cancer including lung cancer. Here, we show, for the first time, that CK2? is a positive regulator of Notch1 signalling in lung cancer cell lines A549 and H1299. We found that Notch1 protein level was reduced after CK2? silencing. Down-regulation of Notch1 transcriptional activity was demonstrated after the silencing of CK2? in lung cancer cells. Furthermore, small-molecule CK2? inhibitor CX-4945 led to a dose-dependent inhibition of Notch1 transcriptional activity. Conversely, forced overexpression of CK2? resulted in an increase in Notch1 transcriptional activity. Finally, the inhibition of CK2? led to a reduced proportion of stem-like CD44 + /CD24- cell population. Thus, we report that the inhibition of CK2? down-regulates Notch1 signalling and subsequently reduces a cancer stem-like cell population in human lung cancer cells. Our data suggest that CK2? inhibitors may be beneficial to the lung cancer patients with activated Notch1 signalling.
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Direct visualization of a guest-triggered crystal deformation based on a flexible ultramicroporous framework.
Nat Commun
PUBLISHED: 03-18-2013
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Host-guest composites may exhibit abnormal and/or controllable physical properties that are unavailable for traditional solids. However, it is still very difficult to control or visualize the occupancy and motion of the guest. Here we report a flexible ultramicroporous coordination polymer showing exceptional guest-responsive thermal-expansion properties. The vacant crystal exhibits constant and huge thermal expansion over a wide temperature range not only in vacuum but also in air, as its ultramicroporous channel excludes air adsorption even at 77?K. More interestingly, as demonstrated by single-crystal X-ray crystallography, molecular dynamic simulations and solid-state nuclear magnetic resonance, it selectively responds to the molecular rearrangement of N,N-dimethylformamide, leading to conformation reversion of the flexible ligand, which transfers these actions to deform the whole crystal lattice. These results illustrate that combination of ultramicroporous channel and flexible pore surface could be an effective strategy for the utilization of external physical and chemical stimuli.
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Impact of the number of resected lymph nodes on postoperative survival of patients with node-negative oesophageal squamous cell carcinoma.
Eur J Cardiothorac Surg
PUBLISHED: 03-10-2013
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Research on the number of resected lymph nodes (LNs) in the cases of node-negative oesophageal squamous cell carcinoma (ESCC) is inadequate. This study was designed to analyse the prognostic impact of the number of resected LNs on node-negative ESCC.
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A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens.
Dev. Comp. Immunol.
PUBLISHED: 03-06-2013
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NK-lysin is an effector protein of cytotoxic T lymphocytes and natural killer cells. Mammalian NK-lysin is known to possess antibacterial property and antitumor activity. Homologues of NK-lysin have been identified in several teleost species, but the natural function of fish NK-lysin remains essentially unknown. In this study, we identified a NK-lysin, CsNKL1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its expression, genetic organization, and biological effect on pathogen infection. CsNKL1 is composed of 135 residues and shares 33.1-56.5% overall sequence identities with other teleost NK-lysin. CsNKL1 possesses a Saposin B domain and six conserved cysteine residues that in mammals are known to form three intrachain disulfide bonds essential to antimicrobial activity. The genomic sequence of the ORF region of CsNKL1 is 1240bp in length and, like human NK-lysin, contains five exons and four introns. Expression of CsNKL1 occurred in multiple tissues and was upregulated by bacterial and viral infection in a time dependent manner. When CsNKL1 was overexpressed in tongue sole, significant upregulation of interleukin-1 and chemokines was observed in spleen and head kidney. Following bacterial and viral infection, the pathogen loads in the tissues of CsNKL1-overexpressing fish were significantly lower than those in control fish. These results indicate that CsNKL1 possesses the novel capacities of immunomodulation and enhancing antimicrobial defense against pathogens of both bacterial and viral nature.
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Association between asymptomatic carotid stenosis and cognitive function: a systematic review.
Neurosci Biobehav Rev
PUBLISHED: 02-20-2013
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Asymptomatic carotid stenosis (CS), traditionally considered clinically silent, may be an independent risk factor for a cognitive impairment.
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Stereotactic Neurosurgical Technique and Electrophysiological Study in Ablating the Ventromedial Shell of the Nucleus Accumbens.
Stereotact Funct Neurosurg
PUBLISHED: 02-19-2013
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Objective: To describe in as much detail as possible the method for ablating the ventromedial shell of the nucleus accumbens (NAc) and investigate the efficacy and safety of the ablation treatment. Methods: Sixty-five patients with drug addictions received operations within the time frame from 2004 to 2009. The ablation targets were located in the bilateral medial posterior inferior shell of the NAc. Intraoperative electrophysiological monitoring was performed. Results: Tissue impedance in the shell of the NAc varied from 185 to 355 ?. When stimulated with a low frequency (2 Hz) and a voltage above 3 V, 57 out of 65 (87.7%) patients experienced slight throbbing sensations. During the lesion procedure, fever was detected on the head and face of 59 patients (90.8%), the heart rate decreased in 19 cases (29.2%), and restlessness, irritability and hyperalgia were noted for all patients. Among the 65 patients, 52 (80%) no longer experienced a psychological craving for the drug. Conclusions: The shell of the NAc may be a promising surgical target for psychosurgery. Electrophysiological recordings revealed that the shell is indeed an appropriate structure. © 2013 S. Karger AG, Basel.
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Assembly of cerium(III)-stabilized polyoxotungstate nanoclusters with SeO3(2-)/TeO3(2-) templates: from single polyoxoanions to inorganic hollow spheres in dilute solution.
Chemistry
PUBLISHED: 02-17-2013
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A versatile one-pot strategy was employed to synthesize three cerium(III)-stabilized polyoxotungstates nanoclusters by combining cerium linkers and SeO3(2-)/TeO3(2-) heteroanion templates: K32Na16[{(XO3)W10O34}8{Ce8(H2O)20}(WO2)4(W4O12)]·nH2O [X=Se, n=81 (1); X=Te, n=114 (2)] and K12Na22[{(SeO3)W10O34}8{Ce8(H2O)20}(WO2)4{(W4O6)Ce4(H2O)14(SeO3)4(NO3)2}]· 79H2O (3), which are the first lanthanide-containing polyoxotungstates with selenium or tellurium heteroatoms. The three clusters were characterized by single-crystal X-ray structure analysis, IR spectroscopy, thermogravimetric/differential thermal analysis, UV/Vis spectroscopy, ESI-MS, and X-ray photoelectron spectroscopy. Their electrochemical, photoluminescence, and magnetic properties were investigated. Their behavior in solution was studied by transmission electron microscopy, which showed that their single polyoxoanions assemble into intact, uniform-sized, purely inorganic hollow spheres in dilute water/acetone solution.
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Genome of the Chinese tree shrew.
Nat Commun
PUBLISHED: 02-07-2013
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Chinese tree shrews (Tupaia belangeri chinensis) possess many features valuable in animals used as experimental models in biomedical research. Currently, there are numerous attempts to employ tree shrews as models for a variety of human disorders: depression, myopia, hepatitis B and C virus infections, and hepatocellular carcinoma, to name a few. Here we present a publicly available annotated genome sequence for the Chinese tree shrew. Phylogenomic analysis of the tree shrew and other mammalians highly support its close affinity to primates. By characterizing key factors and signalling pathways in nervous and immune systems, we demonstrate that tree shrews possess both shared common and unique features, and provide a genetic basis for the use of this animal as a potential model for biomedical research.
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iNOS participates in apoptosis of spinal cord neurons via p-BAD dephosphorylation following ischemia/reperfusion (I/R) injury in rat spinal cord.
Neurosci. Lett.
PUBLISHED: 02-03-2013
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The pro-apoptotic effect of nitric oxide (NO) has been reported both in vivo and in vitro. Previous studies have revealed that NO, especially which produced by inducible nitric oxide synthase (iNOS), has an important effect on apoptosis of neurons in spinal cord ischemia/reperfusion (I/R) injury. To investigate the role of iNOS in this process, a randomized, controlled study was designed using a classical rat model of ischemic spinal cord injury. Fifty-four male Sprague-Dawley rats were randomly divided into three different groups: a sham-operated group (n=6), a vehicle group (I/R, n=24), and an iNOS inhibitor (aminoguanidine: AG) group (I/R+AG, n=24). Rats were sacrificed 6, 12, 24 and 72 h after reperfusion. We examined neurological motor function evaluated by Tarlovs score, assessed alterations in the morphology of spinal cord neurons by transmission electron microscopy (TEM), analyzed expression of iNOS at the levels of mRNA and protein, evaluated local concentrations and cellular locations of other key regulatory proteins, and investigated protein-protein interactions. In the vehicle group, iNOS expression, dephosphorylation of p-BAD (Ser 136), disassociation of BAD from p-BAD/14-3-3 dimers, and release of cytochrome c were all increased compared with the sham group. But in the AG group, all the performances above were decreased compared with the vehicle group. Similarly, rats in the sham group got a maximum score of 5 by Tarlovs motor scores evaluation. While the scores were higher in the AG group compared to the vehicle group because iNOS was inhibited. These results indicate that the activity of iNOS plays a critical role in the apoptosis of spinal cord neurons by influencing the dephosphorylation of p-BAD (Ser 136) and the interaction between BAD and 14-3-3.
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Emergency caudate lobectomy for ruptured hepatocellular carcinoma with multiple primary cancers.
World J. Gastroenterol.
PUBLISHED: 02-02-2013
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We report a case of metachronous multiple primary malignancies involving both rectum and liver with colonic metastasis from hepatocellular carcinoma (HCC) through hematogenous pathway. A 72-year-old woman was admitted to the emergency department with right upper abdominal pain for 4 h. Considering her surgical history of Miles procedure plus liver resection for rectal cancer with liver metastasis three years ago and the finding of urgent computed tomography scan on admission, the preoperative diagnosis was spontaneous rupture of rectal liver metastasis located in caudate lobe and colonic metastasis from rectal cancer. The patient underwent an emergency isolated caudate lobectomy at a hemorrhagic shock status. Pathology reported a primary HCC in the caudate lobe and colonic metastasis of HCC with tumor embolus in the surrounding vessels of the intestine. No regional lymph node involvement was found. It is hypothesized that HCC may disseminate hematogenously to the ascending colon, thus making it a rare case.
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Fish oil-supplemented parenteral nutrition in patients following esophageal cancer surgery: effect on inflammation and immune function.
Nutr Cancer
PUBLISHED: 02-02-2013
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Our aim was to investigate whether adding ?-3 polyunsaturated fatty acids (PUFAs) to parenteral nutrition (PN) could reduce inflammation and improve immune function in patients following esophageal cancer surgery. In this pilot study, 60 patients with esophageal cancer were divided into 2 groups (30 patients in each group). All patients had total scores of more than or equal to 3 on the nutritional risk screening (NRS2002) test recommended by the European Society of Parenteral Enteral Nutrition, which showed that all patients had nutritional risk and should receive nutritional support. Both groups received isocaloric and isonitrogenous PN. One group received a ?-3 PUFAs supplement. Key indicators of inflammation [serum procalcitonin (PCT) level and the ratio of CD4(+) to CD8(+) (CD4(+)/CD8(+) ratio)] were determined intraoperatively and 24, 72, and 144 h postoperatively. PCT level was notably lower and CD4(+)/CD8(+) ratio was markedly higher in the ?-3 PUFAs group (P = 0.007 for PCT level and P = 0.012 for CD4(+)/CD8(+) ratio) on postoperative day 6 but not on postoperative days 1 and 3. ?-3 PUFAs supplemented PN can reduce inflammation and improve immune function in patients following esophageal cancer surgery. A larger trial is required to see whether ?-3 PUFAs supplementation of PN improves the clinical outcomes of patients following esophageal cancer surgery.
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Edaravone protects against glutamate-induced PERK/EIF2?/ATF4 integrated stress response and activation of caspase-12.
Brain Res.
PUBLISHED: 01-29-2013
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As a potent novel free radical scavenger, edaravone has been reported to have neuroprotective effects in both animals and humans, although the underlying mechanisms remain unclear. In our study, we generated a culture of almost pure neurons, which were either left untreated or prophylactically treated with edaravone, then exposed to 50 ?M glutamate for 10 min. Flow Cytometry analysis was performed to quantify the percentage of apoptotic cells. Ultrastructural changes in the endoplasmic reticulum were observed by electron microscopy. Immunofluorescence and western blotting for activation of selected related molecules, including PERK (pancreatic ER stress kinase, PERK), eIF2? (eukaryotic initiation factor 2 alpha, eIF2?), activating ATF4 (transcription factor 4, ATF4), and caspase-12 were examined. In Glutamate-treated group, the sequential activation of PERK, eIF2?, ATF4 and caspase-12 could be observed at 2h, and peaked at 24h. However, treatment with edaravone was able to prevent these changes. In addition, the morphology of the endoplasmic reticulum was better preserved and the percentage of apoptotic cells was lower in cells treated with edaravone. In summary, our results indicate that ISR (PERK/eIF2/ATF4 integrated stress response, ISR) plays an important role in glutamate-induced nerve cells death, and that edaravone could protect neurons against glutamate-induced endoplasmic reticulum stress.
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Surgical approach and outcomes for treatment of pulmonary metastases.
Ann Thorac Med
PUBLISHED: 01-26-2013
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To investigate the surgical approach and outcomes, as well as prognostic factors for pulmonary metastasectomy.
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SIRT1 mediates the protective function of Nkx2.5 during stress in cardiomyocytes.
Basic Res. Cardiol.
PUBLISHED: 01-25-2013
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Nkx2.5 plays protective roles in cardiac homeostasis and survival in the postnatal hearts. However, the underlying molecular mechanisms that mediate the protective functions of Nkx2.5 remain unknown. Here, we showed that Nkx2.5 was downregulated in response to various stresses and was required for protection against the stress-induced apoptosis of cardiomyocytes. SIRT1, a member of the sirtuin family of proteins, was found to be a direct transcriptional target of Nkx2.5 and was required for the Nkx2.5-mediated protection of cardiomyocytes from doxorubicin (DOX)-induced apoptosis. Moreover, using chromatin immunoprecipitation assays, we found that Nkx2.5 was able to bind to the SIRT1 promoter and that this binding was significantly decreased in DOX-treated mouse hearts. Furthermore, the cardiac-specific overexpression of SIRT1 decreased the DOX-induced apoptosis of cardiomyocytes in SIRT1 transgenic mouse hearts compared with the hearts of their wild-type littermates. These findings demonstrate that SIRT1 acts as a direct transcriptional target of Nkx2.5 that maintains cardiomyocyte homeostasis and survival.
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Do tumor location and grade affect survival in pT2N0M0 esophageal squamous cell carcinoma?
J. Thorac. Cardiovasc. Surg.
PUBLISHED: 01-21-2013
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The TNM staging system for esophageal cancer in the seventh edition of the AJCC Cancer Staging Manual incorporates tumor grade and location for staging pT2-3N0M0 esophageal squamous cell carcinoma. Patients with pT2N0M0, classified as stage IIA according to the sixth edition of the AJCC Cancer Staging Manual, can now be classified as stage IB, IIA, or IIB. We discuss whether these changes lead to a better prediction of the prognosis of these patients and aimed to find out other factors to forecast patient prognosis.
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Surgical treatment for limited-stage primary small cell cancer of the esophagus.
Ann. Thorac. Surg.
PUBLISHED: 01-18-2013
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Primary small cell cancer of the esophagus (PSCCE) is a rare, aggressive, and highly metastatic disease. Surgical intervention, radiotherapy, and chemotherapy have been used alone or in combination to improve survival. This retrospective study tried to evaluate the significance of surgical procedures for the treatment of limited-stage PSCCE.
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The upregulation of dihydropyrimidine dehydrogenase in liver is involved in acquired resistance to 5-fluorouracil.
Eur. J. Cancer
PUBLISHED: 01-11-2013
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Acquired resistance to 5-fluorouracil (5-FU) is one of the important reasons for failure in 5-FU-based chemotherapy. The upregulation of dihydropyrimidine dehydrogenase (DPD) in tumours was reported as an important factor for acquired 5-FU resistance. The aim of this study is to examine whether intra-hepatic DPD was involved in acquired 5-FU resistance.
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Molecular mechanism of the inhibition effect of Lipoxin A4 on corneal dissolving pathology process.
Int J Ophthalmol
PUBLISHED: 01-10-2013
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Excessive dissolve of corneal tissue induced by MMPs which were activated by cytokins and chemokines will lead to corneal ulcer. The molecular mechanism of Lipoxin A4 (LXA4) on corneal collagen degradation in three dimensions was investigated.
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Association between the XRCC1 Arg399Gln Polymorphism and Risk of Cancer: Evidence from 297 Case-Control Studies.
PLoS ONE
PUBLISHED: 01-01-2013
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The Arg399Gln polymorphism in the X-ray cross-complementing group 1 (XRCC1) had been implicated in cancer susceptibility. The previous published data on the association between XRCC1 Arg399Gln polymorphism and cancer risk remained controversial.
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Nanoparticles in the ocular drug delivery.
Int J Ophthalmol
PUBLISHED: 01-01-2013
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Ocular drug transport barriers pose a challenge for drug delivery comprising the ocular surface epithelium, the tear film and internal barriers of the blood-aqueous and blood-retina barriers. Ocular drug delivery efficiency depends on the barriers and the clearance from the choroidal, conjunctival vessels and lymphatic. Traditional drug administration reduces the clinical efficacy especially for poor water soluble molecules and for the posterior segment of the eye. Nanoparticles (NPs) have been designed to overcome the barriers, increase the drug penetration at the target site and prolong the drug levels by few internals of drug administrations in lower doses without any toxicity compared to the conventional eye drops. With the aid of high specificity and multifunctionality, DNA NPs can be resulted in higher transfection efficiency for gene therapy. NPs could target at cornea, retina and choroid by surficial applications and intravitreal injection. This review is concerned with recent findings and applications of NPs drug delivery systems for the treatment of different eye diseases.
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Epidermal growth factor receptor (EGFR)-RAS signaling pathway in penile squamous cell carcinoma.
PLoS ONE
PUBLISHED: 01-01-2013
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Penile Squamous Cell Carcinoma (SCC) is a rare cancer with poor prognosis and limited response to conventional chemotherapy. The genetic and epigenetic alterations of Epidermal Growth Factor Receptor (EGFR)-RAS-RAF signaling in penile SCC are unclear. This study aims to investigate four key members of this pathway in penile SCC. We examined the expression of EGFR and RAS-association domain family 1 A (RASSF1A) as well as the mutation status of K-RAS and BRAF in 150 cases of penile SCC. EGFR and RASSF1A expression was evaluated by immunohistochemistry. KRAS mutations at codons 12 and 13, and the BRAF mutation at codon 600 were analyzed on DNA isolated from formalin fixed paraffin embedded tissues by direct genomic sequencing. EGFR expression was positive in all specimens, and its over-expression rate was 92%. RASSF1A expression rate was only 3.42%. Significant correlation was not found between the expression of EGFR or RASSF1A and tumor grade, pT stage or lymph node metastases. The detection of KRAS and BRAF mutations analysis was performed in 94 and 83 tumor tissues, respectively. We found KRAS mutation in only one sample and found no BRAF V600E point mutation. In summary, we found over-expression of EGFR in the majority cases of penile SCC, but only rare expression of RASSF1A, rare KRAS mutation, and no BRAF mutation in penile SCC. These data suggest that anti-EGFR agents may be potentially considered as therapeutic options in penile SCC.
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[Interspecific allelopathic effect of different organs aqueous extracts of Betula platyphylla and Larix olgensis on their seed germination and seedling growth].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 10-05-2011
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In this paper, the Betula platyphylla root-, branch-, and foliage aqueous extracts and Larix olgensis root-, branch-, foliage-, and bark aqueous extracts over a range of concentrations 5.0, 12.5, 25.0, 50.0, and 100.0 mg x mL(-1) were used to study their interspecific allelopathic effect on the seed germination and seedling growth of the two tree species. All the L. olgensis organs extracts, except its root extracts at concentration 5.0 mg x mL(-1), had inhibition effect on B. platyphylla seed germination rate, which was 54%, 58%, 59%, and 66% under the effects of L. olgensis foliage-, branch-, bark-, and root extracts, respectively, as compared with the control. With increasing concentration, the inhibition effect of L. olgensis root- and branch extracts increased while that of L. olgensis foliage- and bark extracts decreased. The L. olgensis organs extracts, especially the foliage extracts at concentration 100.0 mg x mL(-1), had strong inhibition effect on B. platyphylla seed radicle- and hypocotyl length growth, with a decrement of 38% and 55% (P < 0.05), respectively. L. olgensis branch- and foliage extracts promoted, but root- and bark extracts inhibited B. platyphylla seedling growth and biomass production. B. platyphylla organs extracts promoted L. olgensis seed germination, root- and branch extracts promoted hypocotyl length growth, but foliage extracts at 50.0 and 100.0 mg x mL(-1) decreased the hypocotyl length growth by 27% and 28% (P < 0.05), respectively. B. platyphylla organs extracts mainly promoted L. olgensis seedling growth, with the height- and collar diameter growth and biomass accumulation at B. platyphylla foliage extracts concentration 5.0 mg x mL(-1) increased by 54%, 60%, and 100% (P < 0.05), respectively. Our results suggested that there existed obvious allelopathic effect between B. platyphylla and L. olgensis, and thus, mixed planting B. platyphylla and L. olgensis could have promotion effects on the growth of the two tree species.
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Controllable synthesis of flake-like Al-doped ZnO nanostructures and its application in inverted organic solar cells.
Nanoscale Res Lett
PUBLISHED: 05-08-2011
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Flake-like Al-doped ZnO (AZO) nanostructures including dense AZO nanorods were obtained via a low-temperature (100°C) hydrothermal process. By doping and varying Al concentrations, the electrical conductivity (?) and morphology of the AZO nanostructures can be readily controlled. The effect of ? and morphology of the AZO nanostructures on the performance of the inverted organic solar cells (IOSCs) was studied. It presents that the optimized power conversion efficiency of the AZO-based IOSCs is improved by approximately 58.7% compared with that of un-doped ZnO-based IOSCs. This is attributed to that the flake-like AZO nanostructures of high ? and tunable morphology not only provide a high-conduction pathway to facilitate electron transport but also lead to a large interfacial area for exciton dissociation and charge collection by electrodes.
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Hand-assisted thoracoscopic surgery for bilateral lung metastasectomy through sternocostal triangle access.
Ann. Thorac. Surg.
PUBLISHED: 03-01-2011
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Complete resection of pulmonary metastases remains the standard of care for selected patients. An open approach causes criticism because of aggressiveness. Without manual palpation the video-assisted thoracic surgery procedure has the high probability of missing occult metastases. Transxiphoid bilateral lung metastasectomy surgery has not been widely adopted. We developed a hand-assisted thoracoscopic surgery for bilateral lung metastasectomy through natural sternocostal triangle access in 2001 to solve the problems mentioned previously.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.